AU2016328645C1 - Compositions and methods for synthesizing 5'-Capped RNAs - Google Patents

Compositions and methods for synthesizing 5'-Capped RNAs Download PDF

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Publication number
AU2016328645C1
AU2016328645C1 AU2016328645A AU2016328645A AU2016328645C1 AU 2016328645 C1 AU2016328645 C1 AU 2016328645C1 AU 2016328645 A AU2016328645 A AU 2016328645A AU 2016328645 A AU2016328645 A AU 2016328645A AU 2016328645 C1 AU2016328645 C1 AU 2016328645C1
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Prior art keywords
template
oligonucleotide primer
initiating
capped oligonucleotide
independently
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AU2016328645B2 (en
AU2016328645A1 (en
Inventor
Richard I. Hogrefe
Alexandre Lebedev
Anton P. Mccaffrey
Dongwon Shin
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Trilink Biotechnologies LLC
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Trilink Biotechnologies LLC
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Priority to AU2023201915A priority patent/AU2023201915B2/en
Priority to AU2023229522A priority patent/AU2023229522B2/en
Priority to AU2025203425A priority patent/AU2025203425A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/13—Nucleic acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/711—Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7115—Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/712—Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/317—Chemical structure of the backbone with an inverted bond, e.g. a cap structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/336—Modified G
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2016328645A 2015-09-21 2016-09-20 Compositions and methods for synthesizing 5'-Capped RNAs Active AU2016328645C1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2021206780A AU2021206780C1 (en) 2015-09-21 2021-07-19 Compositions and methods for synthesizing 5'-Capped RNAs
AU2023201915A AU2023201915B2 (en) 2015-09-21 2023-03-29 Compositions and methods for synthesizing 5'-Capped RNAs
AU2023229522A AU2023229522B2 (en) 2015-09-21 2023-09-13 Compositions and methods for synthesizing 5'-Capped RNAs
AU2025203425A AU2025203425A1 (en) 2015-09-21 2025-05-13 Compositions and methods for synthesizing 5'-Capped RNAs

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562221248P 2015-09-21 2015-09-21
US62/221,248 2015-09-21
PCT/US2016/052670 WO2017053297A1 (en) 2015-09-21 2016-09-20 Compositions and methods for synthesizing 5'-capped rnas

Related Child Applications (1)

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AU2016328645A1 AU2016328645A1 (en) 2018-04-19
AU2016328645B2 AU2016328645B2 (en) 2021-05-13
AU2016328645C1 true AU2016328645C1 (en) 2021-10-28

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AU2016328645A Active AU2016328645C1 (en) 2015-09-21 2016-09-20 Compositions and methods for synthesizing 5'-Capped RNAs
AU2021206780A Active AU2021206780C1 (en) 2015-09-21 2021-07-19 Compositions and methods for synthesizing 5'-Capped RNAs
AU2023201915A Active AU2023201915B2 (en) 2015-09-21 2023-03-29 Compositions and methods for synthesizing 5'-Capped RNAs
AU2023229522A Active AU2023229522B2 (en) 2015-09-21 2023-09-13 Compositions and methods for synthesizing 5'-Capped RNAs
AU2025203425A Pending AU2025203425A1 (en) 2015-09-21 2025-05-13 Compositions and methods for synthesizing 5'-Capped RNAs

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AU2021206780A Active AU2021206780C1 (en) 2015-09-21 2021-07-19 Compositions and methods for synthesizing 5'-Capped RNAs
AU2023201915A Active AU2023201915B2 (en) 2015-09-21 2023-03-29 Compositions and methods for synthesizing 5'-Capped RNAs
AU2023229522A Active AU2023229522B2 (en) 2015-09-21 2023-09-13 Compositions and methods for synthesizing 5'-Capped RNAs
AU2025203425A Pending AU2025203425A1 (en) 2015-09-21 2025-05-13 Compositions and methods for synthesizing 5'-Capped RNAs

Country Status (21)

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US (9) US10913768B2 (https=)
EP (9) EP3352584B1 (https=)
JP (4) JP6814997B2 (https=)
KR (3) KR102500198B1 (https=)
CN (8) CN120842296A (https=)
AU (5) AU2016328645C1 (https=)
CA (2) CA3218662A1 (https=)
CY (1) CY1124306T1 (https=)
DK (6) DK3954225T5 (https=)
ES (5) ES2879686T3 (https=)
FI (5) FI4140491T3 (https=)
HK (1) HK1255236A1 (https=)
HR (1) HRP20211091T1 (https=)
HU (5) HUE064227T2 (https=)
LT (6) LT4424835T (https=)
PL (6) PL4140491T3 (https=)
PT (6) PT4140491T (https=)
RS (1) RS62129B1 (https=)
SI (5) SI3906789T1 (https=)
SM (1) SMT202100396T1 (https=)
WO (1) WO2017053297A1 (https=)

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