AU2016227607B2 - Cell culture method for mesenchymal stem cells - Google Patents

Cell culture method for mesenchymal stem cells Download PDF

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Publication number
AU2016227607B2
AU2016227607B2 AU2016227607A AU2016227607A AU2016227607B2 AU 2016227607 B2 AU2016227607 B2 AU 2016227607B2 AU 2016227607 A AU2016227607 A AU 2016227607A AU 2016227607 A AU2016227607 A AU 2016227607A AU 2016227607 B2 AU2016227607 B2 AU 2016227607B2
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cell culture
pdgf
culture medium
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AU2016227607A1 (en
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Paul Simmons
Colby SUIRE
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Mesoblast International SARL
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Mesoblast International SARL
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AU2016227607A 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells Active AU2016227607B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2021254538A AU2021254538B2 (en) 2015-03-04 2021-10-19 Cell culture method for mesenchymal stem cells

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2015900752A AU2015900752A0 (en) 2015-03-04 Cell culture method
AU2015900752 2015-03-04
AU2015900777 2015-03-05
AU2015900777A AU2015900777A0 (en) 2015-03-05 Cell culture method
PCT/EP2016/054640 WO2016139340A1 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Related Child Applications (1)

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AU2021254538A Division AU2021254538B2 (en) 2015-03-04 2021-10-19 Cell culture method for mesenchymal stem cells

Publications (2)

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AU2016227607A1 AU2016227607A1 (en) 2017-09-07
AU2016227607B2 true AU2016227607B2 (en) 2021-09-30

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AU2016227607A Active AU2016227607B2 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells
AU2021254538A Active AU2021254538B2 (en) 2015-03-04 2021-10-19 Cell culture method for mesenchymal stem cells

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AU2021254538A Active AU2021254538B2 (en) 2015-03-04 2021-10-19 Cell culture method for mesenchymal stem cells

Country Status (10)

Country Link
US (4) US20180037867A1 (OSRAM)
EP (2) EP4008775A1 (OSRAM)
JP (3) JP6885870B2 (OSRAM)
KR (1) KR102635212B1 (OSRAM)
CN (2) CN113957043A (OSRAM)
AU (2) AU2016227607B2 (OSRAM)
BR (1) BR112017018885A2 (OSRAM)
CA (1) CA2977578C (OSRAM)
SG (1) SG11201706886PA (OSRAM)
WO (1) WO2016139340A1 (OSRAM)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3584310B1 (en) * 2008-09-16 2024-04-17 Mayo Foundation for Medical Education and Research Compositions containing platelet contents
EP4663241A2 (en) 2010-10-08 2025-12-17 Mesoblast International Sàrl Enhanced msc preparations
EP3143130B1 (en) 2014-05-16 2024-03-06 Mayo Foundation for Medical Education and Research Cell culture media compositions for primary cells
EP4008775A1 (en) 2015-03-04 2022-06-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells
WO2019102268A1 (en) 2017-11-22 2019-05-31 Mesoblast International Sarl Cellular compositions and methods of treatment i
CN108251358B (zh) * 2017-12-15 2021-10-26 广东药科大学 一种相同供体来源人间充质干细胞的多批次原代分离方法
SG11202009393QA (en) * 2018-03-28 2020-10-29 Pluristem Ltd Methods for expanding adherent stromal cells and cells obtained thereby
CN108624554B (zh) * 2018-05-14 2021-09-14 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
US11364323B2 (en) 2018-09-17 2022-06-21 Rejuvablast LLC Combination grafts for tissue repair or regeneration applications
JPWO2020067443A1 (ja) * 2018-09-27 2021-08-30 テルモ株式会社 体細胞のシート化方法
KR20230022242A (ko) * 2020-06-11 2023-02-14 메조블라스트 인터내셔널 에스에이알엘 중간엽 계통 전구세포 또는 줄기세포의 3차원 배양
US20240150724A1 (en) * 2021-03-10 2024-05-09 Terasaki Institute For Biomedical Innovation Production of heme for cell-based meat products
CN117529234A (zh) * 2021-03-10 2024-02-06 寺崎生物医学创新研究所 生产产品例如动物源性产品的方法和系统
AU2023389703A1 (en) 2022-12-09 2025-07-10 Mesoblast International Sarl Conditioned media and use of the same
AU2023389700A1 (en) 2022-12-09 2025-07-10 Mesoblast International Sarl Method of treating heart failure in subjects with persistent inflammation
CN120603931A (zh) 2022-12-09 2025-09-05 迈索布拉斯特国际有限公司 预先许可的组合物和细胞培养方法
CN120641115A (zh) 2022-12-09 2025-09-12 迈索布拉斯特国际有限公司 用细胞组合物治疗炎症的方法
WO2024225602A1 (ko) * 2023-04-24 2024-10-31 파미셀 주식회사 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법
WO2025046491A1 (en) 2023-08-28 2025-03-06 Mesoblast International Sàrl Third line treatment for gvhd
WO2025062365A1 (en) 2023-09-22 2025-03-27 Mesoblast International Sárl Mesenchymal lineage precursor or stem cells (mlpscs) for use in methods of treating congenital heart disease

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008020815A1 (en) * 2006-08-15 2008-02-21 Agency For Science, Technology And Research Mesenchymal stem cell conditioned medium
JP2008148643A (ja) * 2006-12-19 2008-07-03 Olympus Corp 無血清培地および幹細胞の培養方法
US20090232777A1 (en) * 2006-03-01 2009-09-17 Evy Lundgren-Akerlund Expansion and Differentiation of Mesenchymal Stem Cells
US20100261274A1 (en) * 2007-09-25 2010-10-14 Vodyanyk Maksym A Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
WO2011159359A2 (en) * 2010-06-17 2011-12-22 Stemrd, Inc. Serum-free chemically defined cell culture medium
US20120177614A1 (en) * 2011-01-12 2012-07-12 Kido Tsuneo Culture method to obtain and maintain a pure or enriched population of mammalian neural stem cells and/or neural/progenitor cells that are prone to differentiate into oligodendrocyte-lineage cells in vitro
EP2545928A1 (en) * 2010-03-10 2013-01-16 Two Cells Co., Ltd Cell preparation containing mesenchymal stem cells, and method for producing same

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
EP0598778B1 (de) 1991-08-12 2001-11-14 Karl Storz GmbH & Co. KG Hochfrequenzchirurgiegenerator zum geregelten schneiden und koagulieren
US5814464A (en) 1994-10-07 1998-09-29 Regeneron Pharma Nucleic acids encoding TIE-2 ligand-2
US6204053B1 (en) 1994-11-08 2001-03-20 Diacrin, Inc. Porcine cortical cells and their use in treatment of neurological deficits due to neurodegenerative diseases
US6277372B1 (en) 1994-11-08 2001-08-21 Diacrin, Inc. Porcine neural cells and their use in treatment of neurological deficits due to neurodegenerative diseases
WO1996031598A1 (en) 1995-04-06 1996-10-10 Regeneron Pharmaceuticals, Inc. Tie-2 ligands, methods of making and uses thereof
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
US5968829A (en) 1997-09-05 1999-10-19 Cytotherapeutics, Inc. Human CNS neural stem cells
AU758073B2 (en) * 1998-11-09 2003-03-13 Consorzio Per La Gestione Del Centro Di Biotecnologie Avanzate Serum free medium for chondrocyte-like cells
AU2003901668A0 (en) 2003-03-28 2003-05-01 Medvet Science Pty. Ltd. Non-haemopoietic precursor cells
WO2001028574A2 (en) 1999-10-20 2001-04-26 Stem Cells, Inc. Methods for inducing in vivo proliferation and migration of transplanted progenitor cells in the brain
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
WO2002064755A2 (en) 2001-02-14 2002-08-22 Anthrogenesis Corporation Post-partum mammalian placenta, its use and placental stem cells therefrom
AU2002243980B2 (en) 2001-02-14 2007-08-23 Robert J. Hariri Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells
EP1444326A4 (en) 2001-08-24 2006-06-28 Advanced Cell Tech Inc SCREENING ASSAYS FOR IDENTIFICATION OF AGENTS INDUCING DIFFERENTIATION, AND PRODUCTION OF CELLS DIFFERENTIATED FOR CELL THERAPY
KR20110036114A (ko) 2002-02-13 2011-04-06 안트로제네시스 코포레이션 산후 포유류 태반으로부터 유래한 배아-유사 줄기 세포와 그 세포를 사용한 용도 및 치료방법
AU2003273573A1 (en) 2002-05-28 2003-12-19 Becton, Dickinson And Company Expansion and transdifferentiation of human acinar cells
WO2003100026A2 (en) 2002-05-28 2003-12-04 Novocell, Inc. Methods, compositions, and growth and differentiation factors for insulin-producing cells
US8491883B2 (en) 2003-06-27 2013-07-23 Advanced Technologies And Regenerative Medicine, Llc Treatment of amyotrophic lateral sclerosis using umbilical derived cells
AU2004252568B2 (en) 2003-06-27 2011-06-30 Ethicon, Incorporated Regeneration and repair of neural tissue using postpartum-derived cells
KR100840979B1 (ko) 2004-02-26 2008-06-24 리라이언스 라이프 사이언시스 프라이빗. 리미티드 각막 윤부에서 유도된 다능성 배아 유사 줄기 세포, 분리방법 및 이들의 용도
WO2006071773A2 (en) 2004-12-23 2006-07-06 Ethicon Incoporated Treatment of osteochondral diseases using postpartum-derived cells and products thereof
EP1831355A2 (en) 2004-12-23 2007-09-12 Ethicon, Inc. Soft tissue repair and regeneration using postpartum-derived cells and cell products
PL1831356T3 (pl) 2004-12-23 2017-07-31 DePuy Synthes Products, Inc. Komórki poporodowe pochodzące z tkanki pępowinowej i sposoby ich wytwarzania i stosowania
JP5425400B2 (ja) 2004-12-23 2014-02-26 エシコン・インコーポレイテッド 産褥由来細胞を用いた脳卒中および他の急性神経変性障害の治療
EP1943334A1 (en) 2005-09-02 2008-07-16 Agency for Science, Technology and Research Method of deriving progenitor cell line
KR101107835B1 (ko) 2006-01-13 2012-02-09 가부시키가이샤 투셀 동물 세포의 무혈청 배양에 사용하기 위한 배양 배지 첨가물, 키트 및 이의 용도
PL2120977T3 (pl) 2007-02-12 2013-12-31 Anthrogenesis Corp Leczenie chorób zapalnych z zastosowaniem łożyskowych komórek macierzystych
WO2008151390A1 (en) 2007-06-15 2008-12-18 Australian Stem Cell Centre Ltd Differentiation of human embryonic stem cells
KR101224588B1 (ko) * 2008-01-24 2013-01-22 삼성전자주식회사 멀티포인트 스트록을 감지하기 위한 ｕｉ 제공방법 및 이를적용한 멀티미디어 기기
US20110110888A1 (en) 2008-06-27 2011-05-12 Hai-Qing Xian Scaffold Coated and/or Impregnated with at Least One Bioactive Agent for Tissue Repair and Other Medical Applications
US20100055078A1 (en) * 2008-08-15 2010-03-04 The Government of the U.S.A, as represented by the Department of Veterans Affairs Methods of making a transplantable bone repair system using multipotent stem cells
US20110212523A1 (en) 2008-11-11 2011-09-01 Yukio Kato Differentiation-inducing culture medium additive and use thereof
EP3187581A1 (en) 2008-12-23 2017-07-05 BOCO Silicon Valley, Inc. Target populations of oligodendrocyte precursor cells and methods of making and using same
US10894944B2 (en) 2009-04-10 2021-01-19 Monash University Cell culture media
KR101523040B1 (ko) * 2012-06-26 2015-05-26 라정찬 고농도의 줄기세포 제조방법
WO2014082685A1 (en) * 2012-11-30 2014-06-05 Swiss Stem Cell Foundation Serum-free medium for human mesenchymal stem cells
CN103396984B (zh) * 2013-07-25 2016-02-10 宗兆文 一种利用皮肤来源的祖细胞制备组织工程脊髓的方法
EP4008775A1 (en) 2015-03-04 2022-06-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090232777A1 (en) * 2006-03-01 2009-09-17 Evy Lundgren-Akerlund Expansion and Differentiation of Mesenchymal Stem Cells
WO2008020815A1 (en) * 2006-08-15 2008-02-21 Agency For Science, Technology And Research Mesenchymal stem cell conditioned medium
JP2008148643A (ja) * 2006-12-19 2008-07-03 Olympus Corp 無血清培地および幹細胞の培養方法
US20100261274A1 (en) * 2007-09-25 2010-10-14 Vodyanyk Maksym A Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
EP2545928A1 (en) * 2010-03-10 2013-01-16 Two Cells Co., Ltd Cell preparation containing mesenchymal stem cells, and method for producing same
WO2011159359A2 (en) * 2010-06-17 2011-12-22 Stemrd, Inc. Serum-free chemically defined cell culture medium
US20120177614A1 (en) * 2011-01-12 2012-07-12 Kido Tsuneo Culture method to obtain and maintain a pure or enriched population of mammalian neural stem cells and/or neural/progenitor cells that are prone to differentiate into oligodendrocyte-lineage cells in vitro

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JP7410899B2 (ja) 2024-01-10
AU2016227607A1 (en) 2017-09-07
US20240191200A1 (en) 2024-06-13
US20250129337A1 (en) 2025-04-24
CN107567494A (zh) 2018-01-09
KR20170120627A (ko) 2017-10-31
AU2021254538A1 (en) 2021-11-18
SG11201706886PA (en) 2017-09-28
WO2016139340A1 (en) 2016-09-09
JP6885870B2 (ja) 2021-06-16
BR112017018885A2 (pt) 2018-04-17
CA2977578A1 (en) 2016-09-09
EP3265554B1 (en) 2021-11-17
JP2024001179A (ja) 2024-01-09
CN113957043A (zh) 2022-01-21
CA2977578C (en) 2024-09-10
KR102635212B1 (ko) 2024-02-13
JP2018506986A (ja) 2018-03-15
US20180037867A1 (en) 2018-02-08
EP3265554A1 (en) 2018-01-10
JP2021090463A (ja) 2021-06-17
EP4008775A1 (en) 2022-06-08
US20200040303A1 (en) 2020-02-06
US11685899B2 (en) 2023-06-27
US12215352B2 (en) 2025-02-04
AU2021254538B2 (en) 2024-01-18

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