AU2015326892B2 - Methods and materials for biosynthesis of mogroside compounds - Google Patents

Methods and materials for biosynthesis of mogroside compounds Download PDF

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AU2015326892B2
AU2015326892B2 AU2015326892A AU2015326892A AU2015326892B2 AU 2015326892 B2 AU2015326892 B2 AU 2015326892B2 AU 2015326892 A AU2015326892 A AU 2015326892A AU 2015326892 A AU2015326892 A AU 2015326892A AU 2015326892 B2 AU2015326892 B2 AU 2015326892B2
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leu
mogroside
seq
glu
polypeptide
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AU2015326892A1 (en
AU2015326892A2 (en
Inventor
Soren DAMKIAER
Iver Klavs Riishede HANSEN
Jorgen Hansen
Jens Houghton-Larsen
Katarzyna KRZYSTANEK
Sathish Kumar
Gary Liu
Yaoquan Liu
Muthuswamy Panchapagesa MURALI
Nina Nicoline Rasmussen
Angelika SEMMLER
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Danstar Ferment AG
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Danstar Ferment AG
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Priority claimed from US14/504,109 external-priority patent/US10011859B2/en
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Publication of AU2015326892A2 publication Critical patent/AU2015326892A2/en
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Priority to AU2022204012A priority Critical patent/AU2022204012C1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/14—Hydrolases (3)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04J—MULTIPLEX COMMUNICATION
    • H04J14/00—Optical multiplex systems
    • H04J14/02—Wavelength-division multiplex systems
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Microbiology (AREA)
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  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Computer Networks & Wireless Communication (AREA)
  • Signal Processing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
AU2015326892A 2014-10-01 2015-09-30 Methods and materials for biosynthesis of mogroside compounds Active AU2015326892B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2022204012A AU2022204012C1 (en) 2014-10-01 2022-06-09 Methods and materials for biosynthesis of mogroside compounds

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US14/504,109 US10011859B2 (en) 2012-12-04 2014-10-01 Methods and materials for biosynthesis of mogroside compounds
US14/504,109 2014-10-01
US201462059136P 2014-10-02 2014-10-02
US62/059,136 2014-10-02
US201462087726P 2014-12-04 2014-12-04
US62/087,726 2014-12-04
US201462090836P 2014-12-11 2014-12-11
US62/090,836 2014-12-11
US201462091895P 2014-12-15 2014-12-15
US62/091,895 2014-12-15
US201562199115P 2015-07-30 2015-07-30
US62/199,115 2015-07-30
PCT/EP2015/072645 WO2016050890A2 (en) 2014-10-01 2015-09-30 Methods and materials for biosynthesis of mogroside compounds

Related Child Applications (1)

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AU2022204012A Division AU2022204012C1 (en) 2014-10-01 2022-06-09 Methods and materials for biosynthesis of mogroside compounds

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AU2015326892A1 AU2015326892A1 (en) 2017-03-30
AU2015326892A2 AU2015326892A2 (en) 2017-05-25
AU2015326892B2 true AU2015326892B2 (en) 2022-03-10

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US (2) US10633685B2 (https=)
EP (1) EP3201315A2 (https=)
JP (1) JP2017529860A (https=)
CN (1) CN107466320B (https=)
AU (2) AU2015326892B2 (https=)
CA (2) CA2963300C (https=)
IL (2) IL251380B (https=)
SG (2) SG10201902813XA (https=)
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Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2972739A1 (en) * 2014-09-11 2016-03-17 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of producing mogrosides and compositions comprising same and uses thereof
CN105801658B (zh) * 2014-12-30 2019-03-19 北京农学院 甜甙及其类似物的制备及作为stat3、erk信号通路靶位点药物在抗肿瘤中的应用
CA3003163A1 (en) 2015-10-29 2017-05-04 Senomyx, Inc. High intensity sweeteners
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
US11060124B2 (en) * 2017-05-03 2021-07-13 Firmenich Incorporated Methods for making high intensity sweeteners
EP3638804A1 (en) 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
CN109402163A (zh) * 2018-09-26 2019-03-01 怀化兴科创生物技术有限公司 新的罗汉果角鲨烯环氧酶基因（Sgsqe）的应用
CN109439676A (zh) * 2018-09-26 2019-03-08 怀化兴科创生物技术有限公司 罗汉果环氧化物水解酶Sgeph5基因的应用
JP2022512931A (ja) * 2018-11-07 2022-02-07 フィルメニッヒ インコーポレイテッド 高甘味度甘味料の製造方法
CA3118467A1 (en) * 2018-11-07 2020-05-14 Firmenich Incorporated Methods for making high intensity sweeteners
EP3877519B1 (en) * 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110066785B (zh) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 一种罗汉果葫芦二烯醇合酶突变体及其构建方法和应用
CN110357936B (zh) * 2019-07-16 2020-10-16 湖南华诚生物资源股份有限公司 半合成罗汉果甜苷v的方法
TWI711385B (zh) * 2019-09-26 2020-12-01 國立臺灣大學 將羅漢果皂苷萃取物生物轉化為賽門苷ｉ之方法
CA3158430A1 (en) * 2019-10-25 2021-04-29 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
WO2021126960A1 (en) * 2019-12-16 2021-06-24 Manus Bio, Inc. Microbial production of mogrol and mogrosides
MX2022011627A (es) * 2020-03-17 2022-12-15 Coca Cola Co Nuevo sistema y métodos de producción de mogrósidos.
CA3191387A1 (en) 2020-09-30 2022-04-07 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
KR20240005708A (ko) * 2021-04-02 2024-01-12 징코 바이오웍스, 인크. 이소프레노이드 및 그의 전구체의 생합성
EP4314272A1 (en) * 2021-04-02 2024-02-07 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN113388590B (zh) * 2021-06-07 2022-02-25 山西农业大学 一种细胞色素P450s的突变体
WO2023278976A2 (en) 2021-06-29 2023-01-05 Firmenich Incorporated Methods for making high intensity sweeteners
CN114854722B (zh) * 2022-05-11 2024-01-09 成都普睿法药物研发有限公司 一种季也蒙念珠菌外切β-1,3-葡聚糖酶及其突变体及应用
NL2032683B1 (en) * 2022-07-18 2024-01-26 Sestina Bio Llc Bioproduction of isoprenoids
US20240229055A9 (en) * 2022-09-19 2024-07-11 Elo Life Systems Mogroside compositions and methods of producing same
CN115896136B (zh) * 2022-12-22 2025-12-16 云南农业大学 与葫芦二烯醇羟化相关的细胞色素p450基因及其编码产物
WO2024218244A1 (en) 2023-04-20 2024-10-24 Dsm Ip Assets B.V. Strains and methods for the production of mogrosides
CN117417949B (zh) * 2023-10-19 2024-12-20 云南农业大学 雪胆细胞色素氧化酶HcCYP81Q58转基因酵母工程菌及其应用
WO2025144795A1 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds
CN117887599A (zh) * 2024-01-11 2024-04-16 桂林莱茵合成生物技术有限公司 一种从头合成罗汉果甜苷的工程酵母的构建方法
US20250290110A1 (en) * 2024-03-18 2025-09-18 Elo Life Systems Mogroside compositions and methods of producing same
CN118185998A (zh) * 2024-04-17 2024-06-14 上海吉罗恩生物科技有限公司 一种表达系统及其使用方法、用途
CN118995457A (zh) * 2024-08-15 2024-11-22 北京化工大学 生产罗汉果甜苷的酿酒酵母工程菌及其构建方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014086842A1 (en) * 2012-12-04 2014-06-12 Evolva Sa Methods and materials for biosynthesis of mogroside compounds

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399321B1 (en) 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US7569389B2 (en) 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2437573C2 (ru) 2005-11-23 2011-12-27 Дзе Кока-Кола Компани Композиция интенсивного подсластителя с кальцием и подслащенные ею композиции
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
WO2008062165A2 (en) 2006-11-22 2008-05-29 The University Of York Monoterpenoid modifying enzymes
US20100132073A1 (en) 2006-12-01 2010-05-27 The University Of York Sesquiterpenoid modifying enzymes
CA2725175A1 (en) * 2008-05-23 2009-11-26 Cibus Oils, Llc Production of squalene using yeast
GB0904639D0 (en) 2009-03-18 2009-04-29 Univ York Assay
EP2575432B1 (en) 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
BR112014012543A2 (pt) * 2011-11-23 2017-06-13 Evolva Sa métodos e materiais para síntese enzimática de compostos de mogrosídeo
EP2885420B1 (en) 2012-08-17 2018-12-05 Evolva SA Increased production of terpenes and terpenoids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014086842A1 (en) * 2012-12-04 2014-06-12 Evolva Sa Methods and materials for biosynthesis of mogroside compounds

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EP3201315A2 (en) 2017-08-09
US11091787B2 (en) 2021-08-17
AU2015326892A1 (en) 2017-03-30
CN107466320B (zh) 2021-11-05
IL251380B (en) 2020-09-30
US10633685B2 (en) 2020-04-28
CA2963300A1 (en) 2016-04-07
JP2017529860A (ja) 2017-10-12
AU2022204012B2 (en) 2024-06-27
IL276781A (en) 2020-10-29
CN107466320A (zh) 2017-12-12
SG10201902813XA (en) 2019-04-29
AU2022204012C1 (en) 2024-09-05
SG11201702123SA (en) 2017-04-27
WO2016050890A3 (en) 2016-06-30
US20170247735A1 (en) 2017-08-31
US20200325517A1 (en) 2020-10-15
WO2016050890A2 (en) 2016-04-07
AU2015326892A2 (en) 2017-05-25
CA3232630A1 (en) 2016-04-07
AU2022204012A1 (en) 2022-06-30
IL251380A0 (en) 2017-05-29
CA2963300C (en) 2024-04-30

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