AU2014232314B2 - Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set - Google Patents

Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set Download PDF

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AU2014232314B2
AU2014232314B2 AU2014232314A AU2014232314A AU2014232314B2 AU 2014232314 B2 AU2014232314 B2 AU 2014232314B2 AU 2014232314 A AU2014232314 A AU 2014232314A AU 2014232314 A AU2014232314 A AU 2014232314A AU 2014232314 B2 AU2014232314 B2 AU 2014232314B2
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adaptive immune
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AU2014232314A1 (en
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Harlan S. Robins
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Adaptive Biotechnologies Corp
Fred Hutchinson Cancer Center
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Fred Hutchinson Cancer Center
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Cell Biology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2014232314A 2013-03-15 2014-03-17 Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set Active AU2014232314B2 (en)

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US201361789408P 2013-03-15 2013-03-15
US61/789,408 2013-03-15
PCT/US2013/045994 WO2013188831A1 (en) 2012-06-15 2013-06-14 Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
AUPCT/US2013/045994 2013-06-14
PCT/US2014/030859 WO2014145992A1 (en) 2013-03-15 2014-03-17 Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set

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AU2014232314B2 true AU2014232314B2 (en) 2020-05-14

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US (2) US20160024493A1 (https=)
EP (1) EP2971105B1 (https=)
JP (1) JP6431895B2 (https=)
KR (1) KR20150132479A (https=)
CN (1) CN105452483B (https=)
AU (2) AU2014232314B2 (https=)
CA (1) CA2906218A1 (https=)
IL (1) IL241394B (https=)
SG (2) SG11201506991VA (https=)
WO (1) WO2014145992A1 (https=)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
EP2387627B1 (en) 2009-01-15 2016-03-30 Adaptive Biotechnologies Corporation Adaptive immunity profiling and methods for generation of monoclonal antibodies
DK2823060T3 (en) 2012-03-05 2018-05-28 Adaptive Biotechnologies Corp Determination of associated immune receptor chains from frequency-matched subunits
CA2872468C (en) 2012-05-08 2020-10-27 Adaptive Biotechnologies Corporation Compositions and methods for measuring and calibrating amplification bias in multiplexed pcr reactions
US10150996B2 (en) 2012-10-19 2018-12-11 Adaptive Biotechnologies Corp. Quantification of adaptive immune cell genomes in a complex mixture of cells
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
WO2015134787A2 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10202640B2 (en) * 2014-05-07 2019-02-12 The Board Of Trustees Of The Leland Stanford Junior University Single cell analysis of T cells using high-throughput multiplex amplification and deep sequencing
EP3517625B1 (en) 2014-05-22 2021-01-20 The University of British Columbia Methods for determining lymphocyte receptor chain pairs
CA2966201A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
WO2016086029A1 (en) 2014-11-25 2016-06-02 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
CN104562215B (zh) * 2014-12-29 2017-03-22 北京诺禾致源科技股份有限公司 扩增子文库的构建方法
US10760120B2 (en) * 2015-01-23 2020-09-01 Qiagen Sciences, Llc High multiplex PCR with molecular barcoding
CA2976580A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
EP3262193A2 (en) * 2015-02-26 2018-01-03 The Broad Institute Inc. T cell balance gene expression, compositions of matters and methods of use thereof
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
AU2016253964B2 (en) * 2015-04-27 2022-07-07 Abvitro Llc Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens
WO2017013170A1 (en) * 2015-07-22 2017-01-26 F. Hoffmann-La Roche Ag Identification of antigen epitopes and immune sequences recognizing the antigens
US10539564B2 (en) 2015-07-22 2020-01-21 Roche Sequencing Solutions, Inc. Identification of antigen epitopes and immune sequences recognizing the antigens
GB201516277D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR libraries
GB201516275D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516276D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516265D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516272D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516270D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516269D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516274D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
SG11201803646YA (en) * 2015-11-04 2018-05-30 Atreca Inc Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
EP3423598B1 (en) * 2016-03-01 2024-05-01 Universal Sequencing Technology Corporation Methods and kits for tracking nucleic acid target origin for nucleic acid sequencing
EP3423595A1 (en) 2016-03-03 2019-01-09 CureVac AG Rna analysis by total hydrolysis
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
US11306356B2 (en) 2016-06-01 2022-04-19 Roche Sequencing Solutions, Inc. Immuno-PETE
CN106283201B (zh) * 2016-09-20 2019-08-06 中国医学科学院肿瘤医院 基于高通量测序的tcr多样性检测和文库构建
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP7360324B2 (ja) 2016-12-23 2023-10-12 ビステラ， インコーポレイテッド 結合ポリペプチドおよびそれを作製する方法
CA3050127A1 (en) * 2017-01-12 2018-07-19 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
EP4050113A1 (en) * 2017-01-17 2022-08-31 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
CN110234772B (zh) * 2017-01-31 2024-01-19 路德维格癌症研究所有限公司 增强的免疫细胞受体测序方法
CN111344416A (zh) 2017-09-01 2020-06-26 生命技术公司 用于免疫组库测序的组合物和方法
CN115927566B (zh) * 2017-09-20 2025-02-18 深圳华大智造科技股份有限公司 一种用于Small RNA的测序方法、测序试剂和应用
KR102503130B1 (ko) * 2017-10-27 2023-02-24 더 리전트 오브 더 유니버시티 오브 캘리포니아 내인성 t 세포 수용체의 표적화된 대체
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
CN108374203B (zh) * 2018-03-01 2021-07-23 艾吉泰康生物科技(北京)有限公司 一种用于双向测序的扩增子文库的构建方法
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
WO2020018836A1 (en) 2018-07-18 2020-01-23 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
US20220002802A1 (en) * 2018-07-18 2022-01-06 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
WO2020041702A1 (en) * 2018-08-24 2020-02-27 Swift Biosciences, Inc. Asymmetric targeted amplification methods
EP3966345A4 (en) * 2019-05-10 2023-06-28 The Chinese University of Hong Kong Primers and assays for linking regions using polymerases
EP3980559A1 (en) * 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
US11149322B2 (en) * 2019-06-07 2021-10-19 Chapter Diagnostics, Inc. Methods and compositions for human papillomaviruses and sexually transmitted infections detection, identification and quantification
US20220308061A1 (en) * 2019-09-03 2022-09-29 The Regents Of The University Of California Method to sequence mrna in single cells in parallel with quantification of intracellular phenotype
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
EP4139486A4 (en) * 2020-04-24 2024-10-16 The Board of Trustees of the Leland Stanford Junior University Novel t-cell specificities and uses thereof
US10941453B1 (en) * 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
WO2022055969A1 (en) * 2020-09-08 2022-03-17 Seqwell, Inc. Sequencing oligonucleotides and methods of use thereof
US11111489B1 (en) 2021-01-06 2021-09-07 Think Therapeutics, Inc. Multiplexed testing of lymphocytes for antigen specificity
JP2024512608A (ja) 2021-03-24 2024-03-19 ジェネンテック， インコーポレイテッド Ｔリンパ球における効率的なｔｃｒ遺伝子編集
WO2022207682A1 (en) * 2021-04-01 2022-10-06 F. Hoffmann-La Roche Ag Immune cell counting of sars-cov-2 patients based on immune repertoire sequencing
CN114561454B (zh) * 2022-03-03 2025-11-14 中国人民解放军陆军军医大学 一种基于高通量测序对TCR β进行定量的方法
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing
TWI878779B (zh) * 2022-10-27 2025-04-01 醫華生技股份有限公司 生物微粒富集設備及其微滴產生器
EP4603595A1 (en) * 2024-02-13 2025-08-20 ImmuneDiscover Sweden AB A method for typing the immune genes and the allelic variants thereof

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US6031091A (en) 1987-09-21 2000-02-29 Gen-Probe Incorporated Non-nucleotide linking reagents for nucleotide probes
KR920003787B1 (ko) 1990-03-05 1992-05-14 한국과학기술 연구원 신규 플라스미드 제조 및 그를 함유한 균주를 배양하여 아이 지 에프-1(igf-1)을 생산하는 방법
JP4137996B2 (ja) 1992-05-06 2008-08-20 ジェン−プローブ・インコーポレイテッド 核酸配列増幅方法，組成物及びキット
US5596090A (en) 1992-07-24 1997-01-21 The United States Of America As Represented By The Secretary Of The Navy Antisense oligonucleotides directed against human VCAM-1 RNA
US5571711A (en) 1993-06-17 1996-11-05 Ludwig Institute For Cancer Research Isolated nucleic acid molecules coding for BAGE tumor rejection antigen precursors
ATE272113T1 (de) 1994-02-16 2004-08-15 Crucell Holland Bv Melanoma-assoziierte antigene, epitope davon und impstoffe gegen melanoma
US5821122A (en) 1995-06-07 1998-10-13 Inserm (Institute Nat'l De La Sante Et De La Recherche . .) Isolated nucleic acid molecules, peptides which form complexes with MHC molecule HLA-A2 and uses thereof
JPH0975090A (ja) 1995-09-13 1997-03-25 Kanegafuchi Chem Ind Co Ltd 細胞付着部材
JP5331476B2 (ja) * 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
CN104195227B (zh) * 2008-11-07 2017-04-12 适应生物技术公司 通过序列分析监测状况的方法
CN102459643B (zh) 2009-06-25 2016-06-01 弗雷德哈钦森癌症研究中心 检测获得性免疫的方法
WO2011106738A2 (en) 2010-02-25 2011-09-01 Fred Hutchinson Cancer Research Center Use of tcr clonotypes as biomarkers for disease
EP4219759A3 (en) * 2010-05-06 2023-09-06 Adaptive Biotechnologies Corporation Monitoring health and disease status using clonotype profiles
CN103069006A (zh) * 2010-07-23 2013-04-24 艾索特里克斯遗传实验室有限责任公司 区别表达的胚胎或母源基因组区的鉴定及其用途
WO2012027503A2 (en) 2010-08-24 2012-03-01 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
WO2012083069A2 (en) * 2010-12-15 2012-06-21 The Board Of Trustees Of The Leland Stanford Junior University Measurement and monitoring of cell clonality
WO2012083225A2 (en) 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
KR20190107761A (ko) * 2011-03-09 2019-09-20 셀 시그널링 테크놀러지, 인크. 모노클로날 항체를 생성하는 방법 및 시약
HRP20211595T1 (hr) * 2011-05-24 2022-01-21 BioNTech SE Individualizirana cjepiva protiv raka
DK2823060T3 (en) * 2012-03-05 2018-05-28 Adaptive Biotechnologies Corp Determination of associated immune receptor chains from frequency-matched subunits

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US20160024493A1 (en) 2016-01-28
EP2971105B1 (en) 2018-08-01
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CA2906218A1 (en) 2014-09-18
WO2014145992A1 (en) 2014-09-18
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