AU2013204758B2 - Humanized rodents that express heavy chains containing VL domains - Google Patents

Humanized rodents that express heavy chains containing VL domains Download PDF

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Publication number
AU2013204758B2
AU2013204758B2 AU2013204758A AU2013204758A AU2013204758B2 AU 2013204758 B2 AU2013204758 B2 AU 2013204758B2 AU 2013204758 A AU2013204758 A AU 2013204758A AU 2013204758 A AU2013204758 A AU 2013204758A AU 2013204758 B2 AU2013204758 B2 AU 2013204758B2
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AU
Australia
Prior art keywords
human
mouse
gene
immunoglobulin
gene segments
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AU2013204758A
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AU2013204758A1 (en
Inventor
Cagan Gurer
Lynn Macdonald
Karolina A. Meagher
Andrew J. Murphy
Sean Stevens
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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Priority to AU2015246154A priority Critical patent/AU2015246154B2/en
Priority to AU2017251848A priority patent/AU2017251848B2/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C07K16/462—Igs containing a variable region (Fv) from one specie and a constant region (Fc) from another
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24046—Adamalysin (3.4.24.46)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/53—Hinge
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds
    • C07K2318/10—Immunoglobulin or domain(s) thereof as scaffolds for inserted non-Ig peptide sequences, e.g. for vaccination purposes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Steroid Compounds (AREA)
  • Housing For Livestock And Birds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Central Heating Systems (AREA)
AU2013204758A 2012-02-01 2013-02-01 Humanized rodents that express heavy chains containing VL domains Active AU2013204758B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2015246154A AU2015246154B2 (en) 2012-02-01 2015-10-23 Humanized rodents that express heavy chains containing VL domains
AU2017251848A AU2017251848B2 (en) 2012-02-01 2017-10-27 Humanized rodents that express heavy chains containing VL domains

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261593463P 2012-02-01 2012-02-01
US61/593,463 2012-02-01
US201261677538P 2012-07-31 2012-07-31
US61/677,538 2012-07-31
PCT/US2013/024295 WO2013116609A1 (en) 2012-02-01 2013-02-01 Humanized rodents that express heavy chains containing vl domains

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2015246154A Division AU2015246154B2 (en) 2012-02-01 2015-10-23 Humanized rodents that express heavy chains containing VL domains

Publications (2)

Publication Number Publication Date
AU2013204758A1 AU2013204758A1 (en) 2013-08-15
AU2013204758B2 true AU2013204758B2 (en) 2015-07-23

Family

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Family Applications (3)

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AU2013204758A Active AU2013204758B2 (en) 2012-02-01 2013-02-01 Humanized rodents that express heavy chains containing VL domains
AU2015246154A Active AU2015246154B2 (en) 2012-02-01 2015-10-23 Humanized rodents that express heavy chains containing VL domains
AU2017251848A Active AU2017251848B2 (en) 2012-02-01 2017-10-27 Humanized rodents that express heavy chains containing VL domains

Family Applications After (2)

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AU2015246154A Active AU2015246154B2 (en) 2012-02-01 2015-10-23 Humanized rodents that express heavy chains containing VL domains
AU2017251848A Active AU2017251848B2 (en) 2012-02-01 2017-10-27 Humanized rodents that express heavy chains containing VL domains

Country Status (26)

Country Link
US (3) US20130212719A1 (enExample)
EP (3) EP3597038B1 (enExample)
JP (6) JP2015505477A (enExample)
KR (2) KR20140123983A (enExample)
CN (2) CN106117364B (enExample)
AU (3) AU2013204758B2 (enExample)
BR (1) BR112014018843B1 (enExample)
CA (1) CA2863175A1 (enExample)
CY (2) CY1122419T1 (enExample)
DK (2) DK2809150T3 (enExample)
ES (2) ES2870703T3 (enExample)
HR (2) HRP20192218T1 (enExample)
HU (2) HUE055610T2 (enExample)
IL (3) IL233827A0 (enExample)
LT (2) LT2809150T (enExample)
ME (1) ME03611B (enExample)
MX (2) MX373286B (enExample)
NZ (5) NZ793447A (enExample)
PL (2) PL2809150T3 (enExample)
PT (2) PT3597038T (enExample)
RS (2) RS59699B1 (enExample)
RU (2) RU2770016C2 (enExample)
SG (3) SG10201606256TA (enExample)
SI (2) SI2809150T1 (enExample)
SM (2) SMT202100401T1 (enExample)
WO (1) WO2013116609A1 (enExample)

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CN116848254B (zh) 2020-12-16 2026-03-17 瑞泽恩制药公司 表达人源化Fcα受体的小鼠
CN114645042A (zh) * 2020-12-18 2022-06-21 中国食品药品检定研究院 一种构建具有表达荧光素酶和红色荧光蛋白的重组巨噬细胞的转基因小鼠的方法
CN114196626A (zh) * 2022-02-16 2022-03-18 北京国卫生物科技有限公司 脐带间充质干细胞及其分离培养扩增方法
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HK1202771A1 (en) 2015-10-09
JP2021137030A (ja) 2021-09-16
SG11201404477YA (en) 2014-08-28
PL3597038T3 (pl) 2021-11-08
NZ627977A (en) 2016-06-24
RU2770016C2 (ru) 2022-04-14
NZ719228A (en) 2022-08-26
HRP20192218T1 (hr) 2020-03-06
JP7525579B2 (ja) 2024-07-30
CY1124361T1 (el) 2022-07-22
JP6942757B2 (ja) 2021-09-29
US20210368751A1 (en) 2021-12-02
LT3597038T (lt) 2021-05-25
JP2018075041A (ja) 2018-05-17
CN104202971B (zh) 2016-07-06
IL261280B (en) 2019-08-29
KR20190006029A (ko) 2019-01-16
EP3912465A1 (en) 2021-11-24
IL268675A (en) 2019-10-31
AU2015246154A1 (en) 2015-11-12
RU2014133653A (ru) 2016-03-20
PT2809150T (pt) 2019-12-09
SMT202100401T1 (it) 2021-09-14
ES2753774T3 (es) 2020-04-14
PL2809150T3 (pl) 2020-04-30
JP2023014242A (ja) 2023-01-26
CA2863175A1 (en) 2013-08-08
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CN106117364A (zh) 2016-11-16
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DK2809150T3 (da) 2019-12-09
SG10201606256TA (en) 2016-09-29
BR112014018843A2 (pt) 2017-07-04
EP2809150B1 (en) 2019-09-18
AU2015246154B2 (en) 2017-11-23
RU2664185C2 (ru) 2018-08-15
BR112014018843A8 (pt) 2019-01-29
BR112014018843B1 (pt) 2024-02-20
MX2019002517A (es) 2019-06-17
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JP2023168590A (ja) 2023-11-24
RU2018122695A (ru) 2019-03-06
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ME03611B (me) 2020-07-20
US20130212719A1 (en) 2013-08-15
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JP2015505477A (ja) 2015-02-23
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HUE055610T2 (hu) 2021-12-28
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PT3597038T (pt) 2021-05-10
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US20170094955A1 (en) 2017-04-06
SI3597038T1 (sl) 2021-07-30
CN106117364B (zh) 2021-08-31
HUE046744T2 (hu) 2020-03-30
EP3597038A1 (en) 2020-01-22
CN104202971A (zh) 2014-12-10
WO2013116609A1 (en) 2013-08-08
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MX373286B (es) 2020-05-20
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HRP20210987T1 (hr) 2021-09-17
LT2809150T (lt) 2019-10-25
NZ734504A (en) 2022-10-28
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