AU2012298799B2 - Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell - Google Patents

Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell Download PDF

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AU2012298799B2
AU2012298799B2 AU2012298799A AU2012298799A AU2012298799B2 AU 2012298799 B2 AU2012298799 B2 AU 2012298799B2 AU 2012298799 A AU2012298799 A AU 2012298799A AU 2012298799 A AU2012298799 A AU 2012298799A AU 2012298799 B2 AU2012298799 B2 AU 2012298799B2
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homologous
cellobiohydrolase
seq
gene
mature polypeptide
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AU2012298799A1 (en
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Qiming Jin
Debbie Yaver
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Novozymes Inc
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Novozymes Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0115—Oligoxyloglucan reducing-end-specific cellobiohydrolase (3.2.1.150)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2012298799A 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell Active AU2012298799B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161526809P 2011-08-24 2011-08-24
US61/526,809 2011-08-24
PCT/US2012/052143 WO2013028912A2 (en) 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell

Publications (2)

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AU2012298799A1 AU2012298799A1 (en) 2014-02-13
AU2012298799B2 true AU2012298799B2 (en) 2018-02-01

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US (3) US9493790B2 (cg-RX-API-DMAC7.html)
EP (1) EP2748322A2 (cg-RX-API-DMAC7.html)
CN (2) CN103890180A (cg-RX-API-DMAC7.html)
AU (1) AU2012298799B2 (cg-RX-API-DMAC7.html)
BR (1) BR112014004190A2 (cg-RX-API-DMAC7.html)
IN (1) IN2014CN02136A (cg-RX-API-DMAC7.html)
WO (1) WO2013028912A2 (cg-RX-API-DMAC7.html)

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WO2016012468A1 (en) 2014-07-21 2016-01-28 Novartis Ag Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
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WO2018093752A1 (en) * 2016-11-15 2018-05-24 Danisco Us Inc. Filamentous fungi with improved protein production
CN110168095A (zh) 2016-12-06 2019-08-23 诺维信公司 用于使用工程化酵母菌株从含有木糖的纤维素基质生产乙醇的改善方法
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MX2021000893A (es) 2018-07-25 2021-03-31 Novozymes As Levadura que expresa enzimas para la producción de etanol.
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CN110724643B (zh) * 2019-11-18 2022-07-15 河南省农业科学院植物保护研究所 一株具有防病促生功能的橘灰青霉及其菌剂和应用
CA3158982A1 (en) 2019-12-10 2021-06-17 Monica TASSONE Microorganism for improved pentose fermentation
CA3191025A1 (en) 2020-09-04 2022-03-10 Roberto Nobuyuki Maeda Improved fermenting organism for ethanol production
AU2022288057A1 (en) 2021-06-07 2023-12-21 Novozymes A/S Engineered microorganism for improved ethanol fermentation
CN114561303B (zh) * 2022-02-14 2023-11-17 大连理工大学 一株分泌高性能纤维素酶的里氏木霉工程菌株及其应用
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WO2025088155A1 (en) 2023-10-25 2025-05-01 Novozymes A/S Improved fermenting organism for ethanol production
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