AU2010322233B2 - Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms - Google Patents

Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms Download PDF

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Publication number
AU2010322233B2
AU2010322233B2 AU2010322233A AU2010322233A AU2010322233B2 AU 2010322233 B2 AU2010322233 B2 AU 2010322233B2 AU 2010322233 A AU2010322233 A AU 2010322233A AU 2010322233 A AU2010322233 A AU 2010322233A AU 2010322233 B2 AU2010322233 B2 AU 2010322233B2
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AU
Australia
Prior art keywords
hemoglobin
antibody
variants
glycated
binding
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Ceased
Application number
AU2010322233A
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English (en)
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AU2010322233A1 (en
Inventor
Benedicte Jardin
Roger Walker
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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Publication of AU2010322233A1 publication Critical patent/AU2010322233A1/en
Application granted granted Critical
Publication of AU2010322233B2 publication Critical patent/AU2010322233B2/en
Priority to AU2017200733A priority Critical patent/AU2017200733B2/en
Priority to AU2019200070A priority patent/AU2019200070B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2010322233A 2009-11-18 2010-11-09 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms Ceased AU2010322233B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2017200733A AU2017200733B2 (en) 2009-11-18 2017-02-03 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms
AU2019200070A AU2019200070B2 (en) 2009-11-18 2019-01-07 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US26248809P 2009-11-18 2009-11-18
US61/262,488 2009-11-18
US12/941,738 US8603828B2 (en) 2009-11-18 2010-11-08 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms
US12/941,738 2010-11-08
PCT/US2010/055952 WO2011062803A2 (en) 2009-11-18 2010-11-09 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2017200733A Division AU2017200733B2 (en) 2009-11-18 2017-02-03 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms

Publications (2)

Publication Number Publication Date
AU2010322233A1 AU2010322233A1 (en) 2012-06-21
AU2010322233B2 true AU2010322233B2 (en) 2016-11-03

Family

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Family Applications (3)

Application Number Title Priority Date Filing Date
AU2010322233A Ceased AU2010322233B2 (en) 2009-11-18 2010-11-09 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms
AU2017200733A Ceased AU2017200733B2 (en) 2009-11-18 2017-02-03 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms
AU2019200070A Ceased AU2019200070B2 (en) 2009-11-18 2019-01-07 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2017200733A Ceased AU2017200733B2 (en) 2009-11-18 2017-02-03 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms
AU2019200070A Ceased AU2019200070B2 (en) 2009-11-18 2019-01-07 Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms

Country Status (8)

Country Link
US (5) US8603828B2 (enExample)
EP (3) EP2502080B1 (enExample)
JP (2) JP6139886B2 (enExample)
AU (3) AU2010322233B2 (enExample)
CA (1) CA2781255C (enExample)
ES (1) ES2665958T3 (enExample)
PT (1) PT3043182T (enExample)
WO (1) WO2011062803A2 (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8603828B2 (en) 2009-11-18 2013-12-10 Bio-Rad Laboratories, Inc. Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
WO2015099123A1 (ja) * 2013-12-26 2015-07-02 京セラ株式会社 センサ
EP3404413B1 (en) 2014-03-20 2020-01-01 Bio-Rad Laboratories, Inc. Glycated protein assay
US10288628B2 (en) 2014-04-11 2019-05-14 Siemens Healthcare Diagnostics Inc. Spectroscopic methods for the detection of glycated hemoglobin
US20160116489A1 (en) * 2014-10-23 2016-04-28 Biomedomics, Inc. Lateral flow immunoassay method of simultaneously detecting hemoglobin s, hemoglobin c, and hemoglobin a in newborns, infants, children, and adults
WO2016109378A1 (en) * 2014-12-29 2016-07-07 North Carolina State University Multiplexed diagnostic to recognize concentrations of related proteins and peptides
CA2975301C (en) 2015-02-09 2025-02-04 Slingshot Biosciences, Inc. Hydrogel particles exhibiting tunable optical characteristics and methods of use
JP6492856B2 (ja) * 2015-03-25 2019-04-03 東ソー株式会社 安定型糖化ヘモグロビンＡ１ｃの定量方法
EP3365012A1 (en) * 2015-10-21 2018-08-29 Qoolabs, Inc. Camelid hemoglobin antibodies and methods of use
US10670615B2 (en) 2015-12-04 2020-06-02 Polymer Technology Systems, Inc. Systems and methods for interference correction from hemoglobin variants
DE112016006444T5 (de) 2016-02-17 2018-11-29 Denso Corporation Luftkonditioniereinheit für ein Fahrzeug
US12345718B2 (en) * 2016-11-04 2025-07-01 Université Paris Est Créteil Val De Marne Method for determining the haemoglobin content of an erythroid cell
US10317413B2 (en) 2016-11-14 2019-06-11 The Board Of Regents Of The University Of Texas System Method and kit for detection of anti-zika virus antibodies
AU2018386295A1 (en) * 2017-12-15 2020-07-02 Bluebird Bio, Inc. Sickled beta globin antibodies
AU2019301750A1 (en) 2018-07-12 2021-01-28 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
CN112740037A (zh) * 2018-09-28 2021-04-30 积水医疗株式会社 糖化血红蛋白(％)测定方法
JP2022504225A (ja) 2018-10-05 2022-01-13 ボード オブ リージェンツ，ザ ユニバーシティ オブ テキサス システム 固相でのｎ末端ペプチド捕捉および解放の方法
EP3942059A4 (en) * 2019-03-04 2022-04-20 Siemens Healthcare Diagnostics, Inc. STABILIZATION OF THE SURFACE POLYSACCHARID AREA OF A DRIED REAGENT
US20200284807A1 (en) 2019-03-05 2020-09-10 Victor Manneh Saturation binding ratiometric assay
CN115004009A (zh) 2020-01-24 2022-09-02 弹弓生物科学公司 用于细胞样校准颗粒的组合物和方法
CA3177834A1 (en) 2020-05-04 2021-11-11 Jeffrey Kim Compositions and methods for passive optical barcoding for multiplexed assays
KR20240116721A (ko) 2021-10-29 2024-07-30 슬링샷 바이오사이언시즈 인코포레이티드 피더 세포 및 합성 항원 제시 세포로서의 하이드로겔 입자
WO2023195348A1 (ja) * 2022-04-04 2023-10-12 デンカ株式会社 抗ヒトヘモグロビンα鎖モノクローナル抗体又はその抗原結合性断片、ヒトヘモグロビン及び／又は糖化ヒトヘモグロビンを検出する方法、ヒトヘモグロビン及び／又は糖化ヒトヘモグロビンの検出キット、並びに、ペプチド
WO2023196898A1 (en) * 2022-04-07 2023-10-12 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Beta globin mimetic peptides and their use
JP2025516304A (ja) 2022-05-05 2025-05-27 スリングショット バイオサイエンシーズ， インコーポレイテッド 血液学のための赤血球模倣物としての作出された粒子およびそれらを含有する組成物
AU2023280490A1 (en) 2022-06-02 2024-12-05 Slingshot Biosciences, Inc. Apoptotic cell mimic
WO2024092161A2 (en) 2022-10-26 2024-05-02 Slingshot Biosciences, Inc. Size-tunable synthetic particles with tunable optical properties and methods for using the same for immune cell activation
WO2025049609A1 (en) 2023-08-29 2025-03-06 Slingshot Biosciences, Inc. Cd34 stem cell mimics

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010021914A2 (en) * 2008-08-21 2010-02-25 Bio-Rad Laboratories, Inc. Calibration surface method for determination of analyte ratios

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2632478A1 (de) 1975-07-23 1977-02-24 Coulter Electronics Verfahren zur erfassung und trennung von antigenen und antikoerperchen im blut und in anderen proben
US4752583A (en) * 1984-11-29 1988-06-21 The United States Of America As Represented By The United States Department Of Energy Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof
US5428754A (en) 1988-03-23 1995-06-27 3Dlabs Ltd Computer system with clock shared between processors executing separate instruction streams
US5922854A (en) * 1991-06-14 1999-07-13 Kumar; Ramesh Purifying Human Hemogloblin from transgenic pig red cells and plasmids containing pig globin nucleic acids
ES2182826T3 (es) * 1992-03-04 2003-03-16 Abbott Lab Determinacion de la hemoglobina glicosilada por reduccion de la intensidad de la fluorescencia.
US6043043A (en) * 1993-04-02 2000-03-28 Bayer Corporation Method for the determination of hemoglobin adducts
US6951716B2 (en) * 1995-03-13 2005-10-04 Mark Hechinger Anti-platelet immunoglobulin bead positive control
US6933106B2 (en) * 1995-03-13 2005-08-23 Mark Hechinger Platelet immunoglobulin bead suspension and flow cytometry
WO1999013907A2 (en) * 1997-09-19 1999-03-25 Serex, Inc. Methods to improve immunogenicity of antigens and specificity of antibodies
JP2000516345A (ja) * 1997-11-18 2000-12-05 バイオ―ラッド ラボラトリーズ インコーポレイテッド 固相として磁性粒子を用いる多重フロー免疫検定
US7271009B1 (en) * 1997-11-18 2007-09-18 Bio-Rad Laboratories, Inc. Multi-analyte diagnostic test for thyroid disorders
JP4318859B2 (ja) * 1997-12-04 2009-08-26 ジーイー・ヘルスケア・ユーケイ・リミテッド 複合アッセイ方法
US20020115116A1 (en) * 2001-02-22 2002-08-22 Yong Song Multiplex protein interaction determinations using glutathione-GST binding
ATE453668T1 (de) * 2001-04-26 2010-01-15 Dako Denmark As Pan-spezifischer monoklonaler antikörper der gegen glycosiliertes hämoglobin gerichtet ist
US20040101874A1 (en) * 2002-04-12 2004-05-27 Mitokor Inc. Targets for therapeutic intervention identified in the mitochondrial proteome
US7326573B2 (en) * 2003-01-10 2008-02-05 Beckman Coulter, Inc. Assay procedures and apparatus
WO2004074452A2 (en) * 2003-02-19 2004-09-02 The Regents Of The University Of California Multiplex mapping of protein interactions
US20040214243A1 (en) * 2003-04-25 2004-10-28 Beckman Coulter, Inc. Differential determination of hemoglobins
US7150995B2 (en) * 2004-01-16 2006-12-19 Metrika, Inc. Methods and systems for point of care bodily fluid analysis
US20080108144A1 (en) * 2004-05-27 2008-05-08 Alam Muhammad A Method for the Rapid Analysis of Polypeptides
JP2006052480A (ja) 2004-08-10 2006-02-23 Nisshinbo Ind Inc セルロース系繊維含有布帛の加工方法及びセルロース系繊維含有布帛
GB0502068D0 (en) * 2005-02-01 2005-03-09 King S College London Screening method
US20080166769A1 (en) 2005-06-03 2008-07-10 Tadayuki Uno High-Copy-Number, High-Expression Vector Having Methionine Aminopeptidase Gene
US7521244B2 (en) * 2006-10-26 2009-04-21 Bionostics, Inc. Standard reference solutions
WO2009067421A1 (en) * 2007-11-20 2009-05-28 Siemens Heathcare Diagnostics Inc. Methods for the detection of glycated hemoglobin
WO2011003182A1 (en) * 2009-07-07 2011-01-13 University Of Victoria Innovation And Development Corporation Methods for early detection of blood disorders
US8603828B2 (en) 2009-11-18 2013-12-10 Bio-Rad Laboratories, Inc. Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010021914A2 (en) * 2008-08-21 2010-02-25 Bio-Rad Laboratories, Inc. Calibration surface method for determination of analyte ratios

Also Published As

Publication number Publication date
PT3043182T (pt) 2018-04-23
AU2019200070A1 (en) 2019-01-31
US8603828B2 (en) 2013-12-10
JP6139886B2 (ja) 2017-05-31
EP3301452A1 (en) 2018-04-04
AU2010322233A1 (en) 2012-06-21
CA2781255C (en) 2017-08-01
EP2502080A2 (en) 2012-09-26
US20160068594A1 (en) 2016-03-10
EP3301452B1 (en) 2020-01-08
JP2013511717A (ja) 2013-04-04
US20190018023A1 (en) 2019-01-17
EP2502080B1 (en) 2016-04-13
EP3043182A1 (en) 2016-07-13
EP3043182B1 (en) 2018-01-17
AU2017200733B2 (en) 2018-10-11
ES2665958T3 (es) 2018-04-30
EP2502080A4 (en) 2013-03-27
US20140073532A1 (en) 2014-03-13
US11262366B2 (en) 2022-03-01
JP2016028254A (ja) 2016-02-25
US10088488B2 (en) 2018-10-02
US9213034B2 (en) 2015-12-15
US9605060B2 (en) 2017-03-28
AU2017200733A1 (en) 2017-02-23
CA2781255A1 (en) 2011-05-26
WO2011062803A3 (en) 2011-07-28
AU2019200070B2 (en) 2020-07-16
WO2011062803A2 (en) 2011-05-26
US20110117670A1 (en) 2011-05-19
US20170176462A1 (en) 2017-06-22

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