AU2010277310B2 - Reverse genetics systems - Google Patents

Reverse genetics systems Download PDF

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AU2010277310B2
AU2010277310B2 AU2010277310A AU2010277310A AU2010277310B2 AU 2010277310 B2 AU2010277310 B2 AU 2010277310B2 AU 2010277310 A AU2010277310 A AU 2010277310A AU 2010277310 A AU2010277310 A AU 2010277310A AU 2010277310 B2 AU2010277310 B2 AU 2010277310B2
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virus
bacterial
construct
influenza
segments
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AU2010277310A1 (en
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Philip Dormitzer
Michael Franti
Peter Mason
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Seqirus UK Ltd
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Seqirus UK Ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
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    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16251—Methods of production or purification of viral material
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/24—Vectors characterised by the absence of particular element, e.g. selectable marker, viral origin of replication
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    • C12N2830/00—Vector systems having a special element relevant for transcription

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  • Engineering & Computer Science (AREA)
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  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Pulmonology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AU2010277310A 2009-07-31 2010-07-30 Reverse genetics systems Active AU2010277310B2 (en)

Priority Applications (1)

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AU2015201871A AU2015201871A1 (en) 2009-07-31 2015-04-14 Reverse genetics systems

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US27315109P 2009-07-31 2009-07-31
US61/273,151 2009-07-31
PCT/IB2010/002137 WO2011012999A1 (en) 2009-07-31 2010-07-30 Reverse genetics systems

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AU2010277310B2 true AU2010277310B2 (en) 2015-01-15

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US (2) US9821052B2 (enExample)
EP (1) EP2459722B1 (enExample)
JP (3) JP2013500712A (enExample)
KR (1) KR20120039047A (enExample)
CN (2) CN102666860B (enExample)
AU (1) AU2010277310B2 (enExample)
CA (1) CA2769673A1 (enExample)
WO (1) WO2011012999A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101323582B1 (ko) * 2011-03-17 2013-10-30 충북대학교 산학협력단 Ｖｅｒｏ 세포 유래의 ｐｏｌⅠ 프로모터 및 이를 포함하는 재조합 벡터
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
ES2628301T3 (es) 2012-03-02 2017-08-02 Seqirus UK Limited Recombinación de virus de gripe
EP2925356A2 (en) 2012-12-03 2015-10-07 Novartis AG Reassortant influenza a viren
AU2014208420A1 (en) * 2013-01-23 2015-07-16 Novartis Ag Influenza virus reassortment
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
US10232031B2 (en) * 2013-03-13 2019-03-19 Seqirus UK Limited Influenza virus reassortment
US10434166B2 (en) 2013-03-15 2019-10-08 University Of Maryland, College Park Methods and compositions for in vivo immune stimulation and antigen production
DE202013005130U1 (de) 2013-06-05 2013-09-10 Novartis Ag Influenza Virus Reassortierung
DE202013005100U1 (de) 2013-06-05 2013-08-26 Novartis Ag Influenza Virus Reassortierung
BR112015030582A2 (pt) 2013-06-06 2017-08-29 Novartis Ag Segmento de hemaglutinina da gripe quimérico e de neuraminidase quimérico, proteína de hemaglutinina quimérica, vírus da gripe rearranjado, métodos para preparação de um vírus da gripe rearranjado e para preparação de uma vacina e sistema de expressão
KR101970428B1 (ko) * 2015-03-27 2019-04-18 카딜라 핼쓰캐어 리미티드 재조합 볼거리 바이러스 제릴린2계 백신
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
CA3080656A1 (en) 2017-10-30 2019-05-09 Baxalta GmbH Environmentally compatible detergents for inactivation of lipid-enveloped viruses
JP7517704B2 (ja) * 2019-06-21 2024-07-17 国立大学法人大阪大学 外来遺伝子を安定的に保持する人工組換えｒｎａウイルスの作製方法
CN110305854B (zh) * 2019-07-18 2023-09-15 山东中医药大学 一种携带荧光素酶的重组h3n2亚型流感病毒、构建方法及应用
MX2022006005A (es) 2019-11-18 2022-10-27 Seqirus Pty Ltd Metodo para producir virus de la influenza reagrupados.
CN114395568A (zh) * 2021-10-29 2022-04-26 扬州大学 一种猪流行性腹泻病毒感染性cDNA克隆及其构建方法与应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004022760A1 (en) * 2002-09-04 2004-03-18 Polymun Scientific Immunbiologische Forschung Gmbh Generation of recombinant influenza virus using baculovirus delivery vector
WO2007044024A2 (en) * 2004-11-19 2007-04-19 Wisconsin Alumni Research Foundation Recombinant influenza vectors with tandem transcription units
CN101302499A (zh) * 2008-06-30 2008-11-12 中国科学院广州生物医药与健康研究院 一种流感病毒疫苗种子株的制备方法
WO2010063804A1 (en) * 2008-12-03 2010-06-10 Avir Green Hills Biotechnology Research Development Trade Ag Novel method for generation of rna virus

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU212924B (en) 1989-05-25 1996-12-30 Chiron Corp Adjuvant formulation comprising a submicron oil droplet emulsion
WO1995011700A1 (en) 1993-10-29 1995-05-04 Pharmos Corp. Submicron emulsions as vaccine adjuvants
DE19612967A1 (de) 1996-04-01 1997-10-02 Behringwerke Ag Verfahren zur Vermehrung von Influenzaviren in Zellkultur, sowie die durch das Verfahren erhältlichen Influenzaviren
DE19612966B4 (de) 1996-04-01 2009-12-10 Novartis Vaccines And Diagnostics Gmbh & Co. Kg MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren
TW570803B (en) 1997-04-09 2004-01-11 Duphar Int Res Influenza vaccine
WO1999050419A2 (en) * 1998-03-31 1999-10-07 University Of Maryland Biotechnology Institute A method for generating nonpathogenic, infectious pancreatic necrosis virus (ipnv) from synthetic rna transcripts
DE69937999T2 (de) 1998-06-12 2009-01-29 Avir Green Hills Biotechnology Research Development Trade Ag Interferon induzierende genetisch veränderte attenuierte viren
AU771110B2 (en) 1998-06-12 2004-03-11 Mount Sinai School Of Medicine Of The City University Of New York, The Novel methods and interferon deficient substrates for the propagation of viruses
GB9916794D0 (en) * 1999-07-16 1999-09-22 Isis Innovation In vitro virus reconstitution
NZ517903A (en) 1999-09-24 2003-10-31 Smithkline Beecham Biolog S One dose intranasal influenza virus vaccine with split influenza viral antigens
DE60142506D1 (de) 2000-03-03 2010-08-19 Chemo Sero Therapeut Res Inst In serumfreier kultur verwendbare zelle, kultursuspension und verfahren zur virusproduktion als impfstoff unter verwendung der zelle
DE60137345D1 (de) 2000-04-28 2009-02-26 St Jude Childrens Res Hospital Dna-transfektionssystem zur erzeugung von infektiösen negativsträngigen rna virus
WO2001090340A2 (en) * 2000-05-21 2001-11-29 University Of North Carolina At Chapel Hill Assembly of large viral genomes and chromosomes from subclones
GB0024089D0 (en) 2000-10-02 2000-11-15 Smithkline Beecham Biolog Novel compounds
CA2438942A1 (en) 2001-02-23 2002-09-26 Glaxosmithkline Biologicals S.A. Influenza vaccine formulations for intradermal delivery
US20040096463A1 (en) 2001-02-23 2004-05-20 Nathalie Garcon Novel vaccine
TWI228420B (en) 2001-05-30 2005-03-01 Smithkline Beecham Pharma Gmbh Novel vaccine composition
UA89631C2 (en) 2004-04-05 2010-02-25 Пфайзер Продактс Инк. Vaccine composition
WO2005113756A1 (en) 2004-05-14 2005-12-01 Glaxosmithkline Biologicals S.A. Method
NZ551640A (en) 2004-05-20 2010-05-28 Id Biomedical Corp Process for the production of an influenza vaccine
CN101189326B (zh) 2004-12-23 2013-06-12 米迪缪尼有限公司 用于病毒增殖的非致瘤性mdck细胞系
AU2005318087B2 (en) 2004-12-24 2011-04-21 Abbott Biologicals B.V. Rescue of influenza virus
FR2884255B1 (fr) 2005-04-11 2010-11-05 Vivalis Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe
CA2613284A1 (en) 2005-06-21 2007-01-04 Medimmune Vaccines, Inc. Methods and compositions for expressing negative-sense viral rna in canine cells
US8703095B2 (en) 2005-07-07 2014-04-22 Sanofi Pasteur S.A. Immuno-adjuvant emulsion
CN101365480B (zh) 2005-11-01 2014-11-05 诺华疫苗和诊断有限两合公司 经由β-丙内酯处理的残留细胞DNA水平降低的细胞衍生病毒疫苗
FR2896162B1 (fr) 2006-01-13 2008-02-15 Sanofi Pasteur Sa Emulsion huile dans eau thermoreversible
AU2007240448B8 (en) 2006-04-19 2014-03-06 Medimmune Llc. Methods and compositions for expressing negative-sense viral RNA in canine cells
CN101522218B (zh) 2006-10-12 2012-09-26 葛兰素史密丝克莱恩生物有限公司 包含水包油乳液佐剂的疫苗
PL2121011T3 (pl) 2006-12-06 2014-10-31 Novartis Ag Szczepionki zawierające antygeny czterech szczepów wirusa grypy
EP2045323A1 (en) 2007-10-05 2009-04-08 Avir Green Hills Biotechnology Research Development Trade Ag Linear expression constructs for production of influenza virus particles
MX2010003644A (es) * 2007-10-08 2010-06-23 Synthetic Genomics Inc Ensamble de acidos nucleicos grandes.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004022760A1 (en) * 2002-09-04 2004-03-18 Polymun Scientific Immunbiologische Forschung Gmbh Generation of recombinant influenza virus using baculovirus delivery vector
WO2007044024A2 (en) * 2004-11-19 2007-04-19 Wisconsin Alumni Research Foundation Recombinant influenza vectors with tandem transcription units
CN101302499A (zh) * 2008-06-30 2008-11-12 中国科学院广州生物医药与健康研究院 一种流感病毒疫苗种子株的制备方法
WO2010063804A1 (en) * 2008-12-03 2010-06-10 Avir Green Hills Biotechnology Research Development Trade Ag Novel method for generation of rna virus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Zhang, X. et . al., Journal of Virology, 2009, vol. 83, No. 18, pages 9296-9303. *

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US10610584B2 (en) 2020-04-07
US20120270321A1 (en) 2012-10-25
US9821052B2 (en) 2017-11-21
JP2016019547A (ja) 2016-02-04
CN104862335A (zh) 2015-08-26
EP2459722A1 (en) 2012-06-06
JP2017189179A (ja) 2017-10-19
KR20120039047A (ko) 2012-04-24
AU2010277310A1 (en) 2012-02-23
US20180236058A1 (en) 2018-08-23
CN102666860A (zh) 2012-09-12
CA2769673A1 (en) 2011-02-03
HK1175814A1 (en) 2013-07-12
JP6666311B2 (ja) 2020-03-13
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WO2011012999A1 (en) 2011-02-03

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