AU2009318813B2 - Methods, devices and kits for detecting or monitoring acute kidney injury - Google Patents
Methods, devices and kits for detecting or monitoring acute kidney injury Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/34—Genitourinary disorders
- G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| US11671308P | 2008-11-21 | 2008-11-21 | |
| US61/116,713 | 2008-11-21 | ||
| PCT/IB2009/055299 WO2010058378A1 (en) | 2008-11-21 | 2009-11-23 | Methods, devices and kits for detecting or monitoring acute kidney injury |
Publications (2)
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| AU2009318813A1 AU2009318813A1 (en) | 2011-07-14 |
| AU2009318813B2 true AU2009318813B2 (en) | 2016-11-24 |
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| AU2009318813A Ceased AU2009318813B2 (en) | 2008-11-21 | 2009-11-23 | Methods, devices and kits for detecting or monitoring acute kidney injury |
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| WO (1) | WO2010058378A1 (cg-RX-API-DMAC7.html) |
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| PL2035835T3 (pl) | 2006-05-30 | 2012-05-31 | Antibodyshop As | Sposoby szybkiej oceny ciężkości urazu |
| US8313919B2 (en) | 2007-03-21 | 2012-11-20 | Bioporto Diagnostics A/S | Diagnostic test for renal injury |
| US8846036B2 (en) | 2007-10-19 | 2014-09-30 | Abbott Laboratories | Antibodies that bind to mammalian NGAL and uses thereof |
| US8394606B2 (en) * | 2008-11-05 | 2013-03-12 | Abbott Laboratories | Neutrophil gelatinase-associated lipocalin (NGAL) protein isoforms enriched from urine and recombinant chinese hamster ovary (CHO) cells and related compositions, antibodies, and methods of enrichment, analysis and use |
| EP2568291A1 (en) * | 2011-09-07 | 2013-03-13 | Roche Diagnostics GmbH | L-FABP based diagnosis of kidney injury after an acute event or after a surgical intervention |
| KR20140076571A (ko) * | 2011-09-22 | 2014-06-20 | 유니버시다드 데 로스 안데스 | 급성 신장 손상의 조기 모니터링, 진단 및/또는 예후 방법 |
| RU2679913C2 (ru) * | 2012-10-02 | 2019-02-14 | Сфинготек Гмбх | Способ диагностики или мониторинга почечной функции или диагностики почечной дисфункции |
| CN102928606B (zh) * | 2012-11-16 | 2015-10-07 | 武汉明德生物科技股份有限公司 | 多抗体标记的降钙素原快速检测试剂盒 |
| CN104871004A (zh) * | 2012-12-20 | 2015-08-26 | 诺华股份有限公司 | 急性肾损伤 |
| US9488663B2 (en) | 2013-03-14 | 2016-11-08 | Abbott Point Of Care Inc. | Electrochemical methods and devices for amending urine samples for immunosensor detection |
| US9651547B2 (en) | 2013-03-14 | 2017-05-16 | Abbott Point Of Care Inc. | Electrochemical methods and devices for amending urine samples for immunosensor detection |
| EP3933384A1 (en) | 2013-03-15 | 2022-01-05 | Hycor Biomedical, LLC | Device and associated method for performing luminescence and fluorescence measurements of a sample |
| RU2566709C2 (ru) * | 2013-12-16 | 2015-10-27 | Владимир Борисович Бородулин | Способ ранней диагностики хронической болезни почек |
| EP3224625B1 (en) | 2014-11-25 | 2020-07-29 | F. Hoffmann-La Roche AG | Biomarkers of fast progression of chronic kidney disease |
| CN104479015A (zh) * | 2014-12-04 | 2015-04-01 | 东南大学 | 针对ngal表位的纳米抗体及其应用 |
| CN105911274A (zh) * | 2016-06-12 | 2016-08-31 | 吉林大学 | 一种同步定量检测不同分子形式人中性粒细胞脂质运载蛋白免疫层析装置及其制备方法 |
| CN108802402A (zh) * | 2018-06-15 | 2018-11-13 | 重庆大学 | 一种快速鉴别呼吸道感染类型的试剂盒及其应用 |
| SE547206C2 (en) * | 2024-01-18 | 2025-05-27 | P & M Venge Ab | Urinary biomarker of kidney function |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004088276A2 (en) * | 2003-03-27 | 2004-10-14 | Children's Hospital Medical Center | A method and kit for detecting the early onset of renal tubular cell injury |
| WO2009062520A1 (en) * | 2007-11-15 | 2009-05-22 | Bioporto Diagnostics A/S | Diagnostic use of individual molecular forms of a biomarker |
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| SE9401351D0 (sv) | 1994-04-21 | 1994-04-21 | Venge | A method for diagnosis |
| WO2002031507A2 (en) * | 2000-10-13 | 2002-04-18 | Children's Medical Center Corporation | Non-invasive enzyme screen for tissue remodelling-associated conditions |
| US7056702B2 (en) * | 2002-12-16 | 2006-06-06 | Kimberly Clark Co | Detecting lipocalin |
| CN1791797A (zh) * | 2003-03-27 | 2006-06-21 | 儿童医院医疗中心 | 用于检测肾小管细胞损伤的早发的方法和试剂盒 |
| CN101010001A (zh) * | 2004-05-06 | 2007-08-01 | 哥伦比亚大学受托人 | Ngal用于减少和改善局部缺血和肾中毒损伤 |
| AU2005240190C1 (en) * | 2004-05-06 | 2012-01-19 | Children's Hospital Medical Center | NGAL for reduction and amelioration of ischemic and nephrotoxic injuries |
| US20050272101A1 (en) | 2004-06-07 | 2005-12-08 | Prasad Devarajan | Method for the early detection of renal injury |
| PL1831699T3 (pl) * | 2004-12-20 | 2010-04-30 | Antibodyshop As | Oznaczanie lipokaliny neutrofilowej związanej z żelatynazą (NGAL) jako markera diagnostycznego dla zaburzeń nerek |
| US20070037232A1 (en) | 2005-03-31 | 2007-02-15 | Barasch Jonathan M | Detection of NGAL in chronic renal disease |
| US20080090304A1 (en) | 2006-10-13 | 2008-04-17 | Barasch Jonathan Matthew | Diagnosis and monitoring of chronic renal disease using ngal |
| CN101421622A (zh) * | 2006-02-17 | 2009-04-29 | 儿童医学中心公司 | 作为癌症生物标志物的游离ngal |
| CA2702611A1 (en) * | 2007-10-19 | 2009-04-23 | Abbott Laboratories | Immunoassays and kits for the detection of ngal |
| US20090123970A1 (en) * | 2007-10-19 | 2009-05-14 | Abbott Laboratories | Glycosylated mammalian ngal and use thereof |
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- 2009-11-23 MY MYPI2011002257A patent/MY162697A/en unknown
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004088276A2 (en) * | 2003-03-27 | 2004-10-14 | Children's Hospital Medical Center | A method and kit for detecting the early onset of renal tubular cell injury |
| WO2009062520A1 (en) * | 2007-11-15 | 2009-05-22 | Bioporto Diagnostics A/S | Diagnostic use of individual molecular forms of a biomarker |
Non-Patent Citations (2)
| Title |
|---|
| BENNETT, M., ET AL., Clinical Journal-American Society of Nephrology, 2008, vol. 3, no. 3, pages 665-673. * |
| MISHRA, J., ET AL., The Lancet, 2005, vol. 365, no. 9466, pages 1231-1238 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3141904A1 (en) | 2017-03-15 |
| CN102292637B (zh) | 2015-08-05 |
| KR101939964B1 (ko) | 2019-01-18 |
| CA2744434A1 (en) | 2010-05-27 |
| WO2010058378A1 (en) | 2010-05-27 |
| KR20110103965A (ko) | 2011-09-21 |
| RU2011125320A (ru) | 2012-12-27 |
| IL212988A (en) | 2014-08-31 |
| US20110287455A1 (en) | 2011-11-24 |
| CA2744434C (en) | 2017-10-03 |
| EP3141904B1 (en) | 2019-01-02 |
| JP2012509477A (ja) | 2012-04-19 |
| AU2009318813A1 (en) | 2011-07-14 |
| ES2718731T3 (es) | 2019-07-04 |
| RU2519722C2 (ru) | 2014-06-20 |
| IL212988A0 (en) | 2011-07-31 |
| US9476880B2 (en) | 2016-10-25 |
| KR20190006614A (ko) | 2019-01-18 |
| JP5438770B2 (ja) | 2014-03-12 |
| CN102292637A (zh) | 2011-12-21 |
| EP2362943A1 (en) | 2011-09-07 |
| MY162697A (en) | 2017-07-14 |
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