AU2009281969A1 - Micro-RNA-based compositions and methods for the diagnosis, prognosis and treatment of multiple myeloma - Google Patents
Micro-RNA-based compositions and methods for the diagnosis, prognosis and treatment of multiple myeloma Download PDFInfo
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- AU2009281969A1 AU2009281969A1 AU2009281969A AU2009281969A AU2009281969A1 AU 2009281969 A1 AU2009281969 A1 AU 2009281969A1 AU 2009281969 A AU2009281969 A AU 2009281969A AU 2009281969 A AU2009281969 A AU 2009281969A AU 2009281969 A1 AU2009281969 A1 AU 2009281969A1
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| PCT/US2009/053586 WO2010019694A1 (en) | 2008-08-12 | 2009-08-12 | Micro-rna-based compositions and methods for the diagnosis, prognosis and treatment of multiple myeloma |
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| WO (1) | WO2010019694A1 (enExample) |
Families Citing this family (39)
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| CN102533966B (zh) | 2005-08-01 | 2014-03-12 | 俄亥俄州立大学研究基金会 | 用于乳腺癌的诊断、预后和治疗的基于MicroRNA的方法和组合物 |
| EP2796554A3 (en) | 2005-09-12 | 2014-12-10 | The Ohio State University Research Foundation | Compositions for use in treating BCL2-associated cancers |
| WO2007081720A2 (en) | 2006-01-05 | 2007-07-19 | The Ohio State University Research Foundation | Microrna-based methods and compositions for the diagnosis, prognosis and treatment of lung cancer |
| CN101384273B (zh) | 2006-01-05 | 2013-07-10 | 俄亥俄州立大学研究基金会 | 胰腺内分泌和腺泡肿瘤中的微小rna表达异常 |
| CN103642900B (zh) | 2006-01-05 | 2016-04-13 | 俄亥俄州立大学研究基金会 | 用于诊断和治疗实体癌的基于微小rna的方法和组合物 |
| CN101448958A (zh) | 2006-03-20 | 2009-06-03 | 俄亥俄州立大学研究基金会 | 人巨核细胞生成期间的微小rna指纹 |
| EP2455492B1 (en) | 2006-07-13 | 2013-11-20 | The Ohio State University Research Foundation | Micro-RNA-based methods and compositions for the diagnosis and treatment of colon related diseases |
| EP2061907B1 (en) | 2006-09-19 | 2011-11-23 | The Ohio State University Research Foundation | Tcl1 expression in chronic lymphocytic leukemia (cll) regulated by mir-29 and mir-181 |
| EP2087135B8 (en) | 2006-11-01 | 2013-07-24 | The Ohio State University Research Foundation | Microrna expression signature for predicting survival and metastases in hepatocellular carcinoma |
| WO2008094545A2 (en) | 2007-01-31 | 2008-08-07 | The Ohio State University Research Foundation | Mic orna-based methods and compositions for the treatment of acute myeloid leukemia |
| WO2008153987A2 (en) | 2007-06-08 | 2008-12-18 | The Government Of The United States Of America As Represented By The Secretary Of Department Of Health And Human Services | Methods for determining hepatocellular carcinoma subtype and detecting hepatic cancer stem cells |
| AU2008266014B2 (en) | 2007-06-15 | 2013-06-06 | The Ohio State University Research Foundation | Oncogenic ALL-1 fusion proteins for targeting drosha-mediated microRNA processing |
| WO2009018303A2 (en) | 2007-07-31 | 2009-02-05 | The Ohio State University Research Foundation | Methods for reverting methylation by targeting dnmt3a and dnmt3b |
| ES2627059T3 (es) | 2007-08-03 | 2017-07-26 | The Ohio State University Research Foundation | Regiones ultraconservadas que codifican ARNnc |
| ES2562078T3 (es) | 2007-08-22 | 2016-03-02 | The Ohio State University Research Foundation | Métodos y composiciones para inducir la desregulación de la fosforilación de EphA7 y ERK en leucemias agudas humanas |
| WO2009055773A2 (en) | 2007-10-26 | 2009-04-30 | The Ohio State University Research Foundation | Methods for identifying fragile histidine triad (fhit) interaction and uses thereof |
| WO2009152300A1 (en) | 2008-06-11 | 2009-12-17 | The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services | Use of mir-26 family as a predictive marker of hepatocellular carcinoma and responsiveness to therapy |
| CA2781547A1 (en) | 2009-11-23 | 2011-05-26 | The Ohio State University | Materials and methods useful for affecting tumor cell growth, migration and invasion |
| CN102241773B (zh) * | 2010-05-13 | 2014-05-14 | 四川大学 | 抗骨髓瘤细胞多克隆抗体及其制备方法 |
| WO2012065049A1 (en) | 2010-11-12 | 2012-05-18 | The Ohio State University Research Foundation | Materials and methods related to microrna-21, mismatch repair, and colorectal cancer |
| AU2011329066B2 (en) | 2010-11-15 | 2017-03-09 | The Ohio State University Research Foundation | Controlled release mucoadhesive systems |
| GB2486424A (en) * | 2010-12-13 | 2012-06-20 | Univ Sussex | Markers for plasma cell disorders |
| WO2012122239A1 (en) | 2011-03-07 | 2012-09-13 | The Ohio State University | MUTATOR ACTIVITY INDUCED BY MICRORNA-155 (miR-155) LINKS INFLAMMATION AND CANCER |
| US8945829B2 (en) * | 2011-03-22 | 2015-02-03 | Cornell University | Distinguishing benign and malignant indeterminate thyroid lesions |
| JP2014511677A (ja) * | 2011-03-22 | 2014-05-19 | コーネル・ユニバーシティー | 鑑別困難な良性甲状腺病変と悪性甲状腺病変との識別法 |
| AU2012323924A1 (en) | 2011-10-14 | 2014-05-29 | The Ohio State University | Methods and materials related to ovarian cancer |
| SG11201402610QA (en) | 2011-12-09 | 2014-10-30 | Metavention Inc | Therapeutic neuromodulation of the hepatic system |
| AU2012352265B2 (en) | 2011-12-13 | 2017-02-16 | Ohio State Innovation Foundation | Methods and compositions related to miR-21 and miR-29a, exosome inhibition, and cancer metastasis |
| EP2804960A4 (en) | 2012-01-20 | 2015-08-19 | Univ Ohio State | BREAST CANCER BIOMARK SIGNATURES FOR INVASIVITY AND FORECAST |
| US10047401B2 (en) | 2012-08-20 | 2018-08-14 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Expression protein-coding and noncoding genes as prognostic classifiers in early stage lung cancer |
| US9944991B2 (en) | 2012-11-02 | 2018-04-17 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for diagnosis, prognosis and treatment of hematological malignancies |
| WO2017091865A1 (en) * | 2015-12-03 | 2017-06-08 | Alfred Health | Monitoring treatment or progression of myeloma |
| CN105603087B (zh) * | 2016-02-01 | 2019-03-01 | 中国医学科学院血液病医院（血液学研究所） | 检测多发性骨髓瘤克隆进化的基因探针组合物及试剂盒 |
| EP3481403B1 (en) * | 2016-07-06 | 2022-02-09 | Youhealth Biotech, Limited | Solid tumor methylation markers and uses thereof |
| CN110223733B (zh) * | 2019-04-22 | 2022-02-01 | 福建医科大学附属第一医院 | 一种多发性骨髓瘤预后基因的筛查方法 |
| BR112021021686A2 (pt) * | 2019-05-03 | 2022-03-22 | Dicerna Pharmaceuticals Inc | Moléculas inibidoras de ácido nucleico de fita dupla com fitas senso curtas |
| US20210002698A1 (en) * | 2019-07-02 | 2021-01-07 | Quark Biosciences Taiwan, Inc. | miRNA Receptivity Analysis of the Endometrium |
| CN110564852A (zh) * | 2019-08-06 | 2019-12-13 | 中国医学科学院血液病医院(中国医学科学院血液学研究所) | 与人类多发性骨髓瘤相关的miRNAs表达图谱模型、构建方法及应用 |
| CN113186163A (zh) * | 2021-01-18 | 2021-07-30 | 南昌五元生物科技有限公司 | 一种基于p53突变筛选肿瘤类器官的培养方法 |
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| EP2295604B1 (en) * | 2004-02-09 | 2015-04-08 | Thomas Jefferson University | Diagnosis and treatment of cancers with microRNA located in or near cancer-associated chromosomal features |
| AU2005250432B2 (en) * | 2004-05-28 | 2011-09-15 | Asuragen, Inc. | Methods and compositions involving microRNA |
| CA2857881A1 (en) * | 2004-11-12 | 2006-12-28 | Asuragen, Inc. | Methods and compositions involving mirna and mirna inhibitor molecules |
| US20060185027A1 (en) * | 2004-12-23 | 2006-08-17 | David Bartel | Systems and methods for identifying miRNA targets and for altering miRNA and target expression |
| US8071559B2 (en) * | 2005-05-02 | 2011-12-06 | Cold Spring Harbor Laboratory | Compositions and methods for cancer diagnosis and treatment |
| CN101448958A (zh) * | 2006-03-20 | 2009-06-03 | 俄亥俄州立大学研究基金会 | 人巨核细胞生成期间的微小rna指纹 |
| EP2455492B1 (en) * | 2006-07-13 | 2013-11-20 | The Ohio State University Research Foundation | Micro-RNA-based methods and compositions for the diagnosis and treatment of colon related diseases |
| WO2008036776A2 (en) * | 2006-09-19 | 2008-03-27 | Asuragen, Inc. | Mir-15, mir-26, mir -31,mir -145, mir-147, mir-188, mir-215, mir-216 mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention |
| AU2008220438A1 (en) * | 2007-02-27 | 2008-09-04 | Rosetta Genomics Ltd. | Composition and methods for modulating cell proliferation and cell death |
| US8097427B2 (en) * | 2007-03-16 | 2012-01-17 | Covalx Ag | Direct mass spectrometric analysis of drug candidates targeting protein complexes |
| ES2936256T3 (es) * | 2008-02-01 | 2023-03-15 | Massachusetts Gen Hospital | Uso de microvesículas en el diagnóstico, y pronóstico de enfermedades y afecciones médicas |
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- 2009-08-12 JP JP2011523144A patent/JP2012500389A/ja active Pending
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- 2009-08-12 US US13/058,662 patent/US20110152357A1/en not_active Abandoned
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- 2009-08-12 AU AU2009281969A patent/AU2009281969A1/en not_active Abandoned
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Also Published As
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| JP2012500389A (ja) | 2012-01-05 |
| WO2010019694A1 (en) | 2010-02-18 |
| US20110152357A1 (en) | 2011-06-23 |
| CA2734179A1 (en) | 2010-02-18 |
| CN102149401A (zh) | 2011-08-10 |
| EP2323677A4 (en) | 2013-01-09 |
| EP2323677A1 (en) | 2011-05-25 |
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