AU2006247752B2 - Method and device for conducting biochemical or chemical reactions at multiple temperatures - Google Patents

Method and device for conducting biochemical or chemical reactions at multiple temperatures Download PDF

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Publication number
AU2006247752B2
AU2006247752B2 AU2006247752A AU2006247752A AU2006247752B2 AU 2006247752 B2 AU2006247752 B2 AU 2006247752B2 AU 2006247752 A AU2006247752 A AU 2006247752A AU 2006247752 A AU2006247752 A AU 2006247752A AU 2006247752 B2 AU2006247752 B2 AU 2006247752B2
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Australia
Prior art keywords
reaction
nucleic acid
path
electrowetting
droplet
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AU2006247752A
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AU2006247752A1 (en
Inventor
Michael G. Pollack
Alexander D. Shenderov
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Advanced Liquid Logic Inc
Duke University
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Advanced Liquid Logic Inc
Duke University
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1816—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using induction heating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation
    • B01L2300/1872—Infrared light
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
AU2006247752A 2005-05-11 2006-05-10 Method and device for conducting biochemical or chemical reactions at multiple temperatures Active AU2006247752B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US67971405P 2005-05-11 2005-05-11
US60/679,714 2005-05-11
PCT/US2006/018088 WO2006124458A2 (en) 2005-05-11 2006-05-10 Method and device for conducting biochemical or chemical reactions at multiple temperatures

Publications (2)

Publication Number Publication Date
AU2006247752A1 AU2006247752A1 (en) 2006-11-23
AU2006247752B2 true AU2006247752B2 (en) 2012-04-12

Family

ID=37431850

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2006247752A Active AU2006247752B2 (en) 2005-05-11 2006-05-10 Method and device for conducting biochemical or chemical reactions at multiple temperatures

Country Status (8)

Country Link
US (4) US9517469B2 (enExample)
EP (1) EP1885885A4 (enExample)
JP (2) JP2008539759A (enExample)
KR (1) KR101431775B1 (enExample)
CN (1) CN101287845B (enExample)
AU (1) AU2006247752B2 (enExample)
CA (1) CA2606750C (enExample)
WO (1) WO2006124458A2 (enExample)

Families Citing this family (151)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329545B2 (en) * 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
DK1859330T3 (da) 2005-01-28 2012-10-15 Univ Duke Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort
US9517469B2 (en) 2005-05-11 2016-12-13 Advanced Liquid Logic, Inc. Method and device for conducting biochemical or chemical reactions at multiple temperatures
JP2009536313A (ja) 2006-01-11 2009-10-08 レインダンス テクノロジーズ， インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
WO2009140671A2 (en) 2008-05-16 2009-11-19 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
WO2007123908A2 (en) 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US7851184B2 (en) 2006-04-18 2010-12-14 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification method and apparatus
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
JP5156762B2 (ja) 2007-02-09 2013-03-06 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴アクチュエータデバイスおよび磁性ビーズを使用する方法
WO2008101194A2 (en) 2007-02-15 2008-08-21 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
WO2008112856A1 (en) * 2007-03-13 2008-09-18 Advanced Liquid Logic, Inc. Droplet actuator devices, configurations, and methods for improving absorbance detection
EP2126038B1 (en) 2007-03-22 2015-01-07 Advanced Liquid Logic, Inc. Enzymatic assays for a droplet actuator
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
WO2008124846A2 (en) * 2007-04-10 2008-10-16 Advanced Liquid Logic, Inc. Droplet dispensing device and methods
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US8951732B2 (en) * 2007-06-22 2015-02-10 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
US20090017554A1 (en) * 2007-06-28 2009-01-15 Applera Corporation Detection and mixing in a conduit in integrated bioanalysis systems
JP5547071B2 (ja) * 2007-08-09 2014-07-09 セルラ・インコーポレイテッド 関連付け多パラメーター単一細胞測定および残留する生物学的材料の回収のための方法および装置
WO2009029561A2 (en) 2007-08-24 2009-03-05 Advanced Liquid Logic, Inc. Bead manipulations on a droplet actuator
WO2009032863A2 (en) 2007-09-04 2009-03-12 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US8460528B2 (en) * 2007-10-17 2013-06-11 Advanced Liquid Logic Inc. Reagent storage and reconstitution for a droplet actuator
EP2232535A4 (en) * 2007-12-10 2016-04-13 Advanced Liquid Logic Inc DROPLET ACTUATOR CONFIGURATIONS AND METHODS
CA2709928A1 (en) 2007-12-23 2009-07-09 Advanced Liquid Logic, Inc. Droplet actuator configurations and methods of conducting droplet operations
WO2009137415A2 (en) 2008-05-03 2009-11-12 Advanced Liquid Logic, Inc. Reagent and sample preparation, loading, and storage
US20110097763A1 (en) * 2008-05-13 2011-04-28 Advanced Liquid Logic, Inc. Thermal Cycling Method
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
FR2938849B1 (fr) * 2008-11-24 2013-04-05 Commissariat Energie Atomique Procede et dispositif pour l'analyse genetique
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US8846414B2 (en) 2009-09-29 2014-09-30 Advanced Liquid Logic, Inc. Detection of cardiac markers on a droplet actuator
US9005544B2 (en) * 2009-10-15 2015-04-14 The Regents Of The University Of California Digital microfluidic platform for radiochemistry
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011057197A2 (en) * 2009-11-06 2011-05-12 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel electrophoresis and molecular analysis
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
ES2574055T3 (es) 2009-11-25 2016-06-14 Gen9, Inc. Métodos y aparatos para la reducción de errores en el ADN basada en un chip
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ， インコーポレイテッド デジタル検体分析
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US9248450B2 (en) 2010-03-30 2016-02-02 Advanced Liquid Logic, Inc. Droplet operations platform
US10232374B2 (en) 2010-05-05 2019-03-19 Miroculus Inc. Method of processing dried samples using digital microfluidic device
WO2012012090A2 (en) 2010-06-30 2012-01-26 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
EP3705573A1 (en) 2010-11-12 2020-09-09 Gen9, Inc. Methods and devices for nucleic acids synthesis
CN102095770A (zh) * 2010-11-22 2011-06-15 复旦大学 一种基于数字微流控技术的电化学传感器芯片
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US8339711B2 (en) 2011-04-22 2012-12-25 Sharp Kabushiki Kaisha Active matrix device and method of driving the same
EP2707131B1 (en) 2011-05-09 2019-04-24 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
AU2012253595B2 (en) 2011-05-10 2016-10-20 Advanced Liquid Logic, Inc. Enzyme concentration and assays
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
CN103733059B (zh) 2011-07-06 2016-04-06 先进流体逻辑公司 在微滴执行机构上的试剂储存
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
KR20130009504A (ko) 2011-07-15 2013-01-23 삼성전자주식회사 개구 조절 방법 및 개구 조절 소자
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
US8980075B2 (en) * 2011-07-29 2015-03-17 The Texas A & M University System Digital microfluidic platform for actuating and heating individual liquid droplets
DK3594340T3 (da) 2011-08-26 2021-09-20 Gen9 Inc Sammensætninger og fremgangsmåder til samling med høj nøjagtighed af nukleinsyrer
US20130063953A1 (en) * 2011-09-13 2013-03-14 Den-Hua Lee Light-emitting diode structure
WO2013037284A1 (en) * 2011-09-15 2013-03-21 The Chinese University Of Hong Kong Microfluidic platform and method for controlling the same
JP5919710B2 (ja) * 2011-10-03 2016-05-18 セイコーエプソン株式会社 熱サイクル装置
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use
WO2013078216A1 (en) 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Glucose-6-phosphate dehydrogenase assays
KR101903789B1 (ko) 2012-02-17 2018-10-02 리쿠아비스타 비.브이. 전기 습윤 표시 장치 및 이를 구동하는 방법
WO2013132645A1 (ja) 2012-03-09 2013-09-12 独立行政法人産業技術総合研究所 核酸増幅方法
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
AU2013251701A1 (en) 2012-04-24 2014-10-30 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
AU2013280661A1 (en) 2012-06-25 2015-01-22 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
IN2015DN00359A (enExample) 2012-06-27 2015-06-12 Advanced Liquid Logic Inc
US9863913B2 (en) 2012-10-15 2018-01-09 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
EP2912432B1 (en) 2012-10-24 2018-07-04 Genmark Diagnostics Inc. Integrated multiplex target analysis
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
CN102980930B (zh) * 2012-12-17 2014-11-05 江苏科技大学 一种电润湿性电极的制备方法
EP3450984B1 (en) 2013-01-31 2020-10-07 Luminex Corporation Fluid retention plates and analysis cartridges
EP3520895A1 (en) 2013-03-15 2019-08-07 Genmark Diagnostics Inc. Fluid container with cantilevered lance
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015138343A1 (en) * 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
KR102232094B1 (ko) * 2014-04-25 2021-03-25 버클리 라잇츠, 인크. 동일한 미세유체 장치에의 dep 처리 디바이스들 및 제어가능 전기습윤 디바이스들의 제공
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
WO2015188165A1 (en) 2014-06-06 2015-12-10 The Regents Of The University Of California Self-shielded, benchtop chemistry system
CN106536704B (zh) 2014-07-08 2020-03-06 国立研究开发法人产业技术综合研究所 核酸扩增装置、核酸扩增方法以及核酸扩增用芯片
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
AU2015346527A1 (en) 2014-11-11 2017-06-29 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system
SG11201704558QA (en) 2014-12-08 2017-07-28 Berkeley Lights Inc Microfluidic device comprising lateral/vertical transistor structures and process of making and using same
CN107429281B (zh) 2014-12-31 2022-05-27 维斯比医学公司 用于分子诊断测试的装置和方法
CN105845158A (zh) 2015-01-12 2016-08-10 腾讯科技（深圳）有限公司 一种信息处理方法及客户端
RU2712610C2 (ru) 2015-04-03 2020-01-29 Эбботт Лэборетриз Устройства и способы для анализа образца
CN107690582B (zh) 2015-04-03 2023-10-20 雅培制药有限公司 用于样品分析的装置和方法
US9841402B2 (en) * 2015-04-15 2017-12-12 Sharp Life Science (Eu) Limited Multifunction electrode with combined heating and EWOD drive functionality
AU2016250689B2 (en) 2015-04-22 2021-07-08 Berkeley Lights, Inc. Microfluidic cell culture
US10464067B2 (en) 2015-06-05 2019-11-05 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
US10695762B2 (en) 2015-06-05 2020-06-30 Miroculus Inc. Evaporation management in digital microfluidic devices
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
US10987674B2 (en) 2016-04-22 2021-04-27 Visby Medical, Inc. Printed circuit board heater for an amplification module
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
EP3458597B1 (en) * 2016-05-18 2022-09-07 Roche Diagnostics GmbH Quantitative real time pcr amplification using an electrowetting-based device
AU2017271673B2 (en) 2016-05-26 2022-04-14 Berkeley Lights, Inc. Covalently modified surfaces, kits, and methods of preparation and use
WO2018005843A1 (en) * 2016-06-29 2018-01-04 Digital Biosystems High resolution temperature profile creation in a digital microfluidic device
CN109715781A (zh) 2016-08-22 2019-05-03 米罗库鲁斯公司 用于数字微流控设备中的并行液滴控制的反馈系统
CA3036572A1 (en) 2016-09-19 2018-03-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
CA3049416A1 (en) 2016-12-28 2018-07-05 Miroculus Inc. Digital microfluidic devices and methods
WO2018187476A1 (en) 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
CN110809495A (zh) * 2017-04-26 2020-02-18 伯克利之光生命科技公司 使用具有优化电润湿表面的微流体装置的生物处理系统和方法
US10695761B2 (en) 2017-05-30 2020-06-30 Sharp Life Science (Eu) Limited Microfluidic device with multiple temperature zones and enhanced temperature control
CN113967488B (zh) * 2017-06-21 2024-02-06 光投发现有限公司 微流体分析设备
EP3658908B1 (en) 2017-07-24 2025-11-12 Integra Biosciences AG Digital microfluidics systems and methods with integrated plasma collection device
WO2019046860A1 (en) 2017-09-01 2019-03-07 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS OF USE
AU2018364741B2 (en) 2017-11-09 2021-03-25 Visby Medical, Inc. Portable molecular diagnostic device and methods for the detection of target viruses
US20190262829A1 (en) 2018-02-28 2019-08-29 Volta Labs, Inc. Directing Motion of Droplets Using Differential Wetting
WO2019226919A1 (en) 2018-05-23 2019-11-28 Miroculus Inc. Control of evaporation in digital microfluidics
CA3108408A1 (en) 2018-08-06 2020-02-13 Nicoya Lifesciences Inc. Plasmon resonance (pr) system, instrument, cartridge, and methods and configurations thereof
CN116351489A (zh) 2019-01-31 2023-06-30 米罗库鲁斯公司 非结垢组合物以及用于操控和处理包封的微滴的方法
CN114206499B (zh) 2019-04-08 2024-10-08 米罗库鲁斯公司 多盒式数字微流控装置和使用方法
WO2021016614A1 (en) 2019-07-25 2021-01-28 Miroculus Inc. Digital microfluidics devices and methods of use thereof
CA3151817A1 (en) * 2019-08-27 2021-03-04 Volta Labs, Inc. Methods and systems for droplet manipulation
US11946901B2 (en) 2020-01-27 2024-04-02 Nuclera Ltd Method for degassing liquid droplets by electrical actuation at higher temperatures
CN112675798B (zh) * 2020-12-14 2022-11-08 上海天马微电子有限公司 微流体反应装置及微流体反应驱动方法
CN112588332B (zh) * 2020-12-24 2023-02-10 广东奥素液芯微纳科技有限公司 一种微液滴生成方法和生成系统
JP2024511359A (ja) 2021-03-19 2024-03-13 ビージー リサーチ エルティーディー 熱サイクリングのための装置及び関連する方法
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
WO2024124087A1 (en) * 2022-12-08 2024-06-13 Baebies, Inc. Methods for performing rapid polymerase chain reaction (pcr) protocols in microfluidics system
GB202305080D0 (en) * 2023-04-05 2023-05-17 Anglia Ruskin Univ Higher Education Corporation Methods and devices for nucleic acid amplification

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030082081A1 (en) * 2001-10-24 2003-05-01 Commissariat A L'energie Atomique Device for parallel and synchronous injection for sequential injection of different reagents
EP1510254A2 (de) * 2003-08-30 2005-03-02 Roche Diagnostics GmbH Verfahren und Vorrichtung zur Bestimmung von Analyten in einer Flüssigkeit

Family Cites Families (106)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4390403A (en) * 1981-07-24 1983-06-28 Batchelder J Samuel Method and apparatus for dielectrophoretic manipulation of chemical species
FR2543320B1 (fr) * 1983-03-23 1986-01-31 Thomson Csf Dispositif indicateur a commande electrique de deplacement d'un fluide
US5038852A (en) * 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5503803A (en) * 1988-03-28 1996-04-02 Conception Technologies, Inc. Miniaturized biological assembly
US4911782A (en) * 1988-03-28 1990-03-27 Cyto-Fluidics, Inc. Method for forming a miniaturized biological assembly
GB8917963D0 (en) * 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
GB8926269D0 (en) * 1989-11-21 1990-01-10 Dynal As Plasmid
US5181016A (en) * 1991-01-15 1993-01-19 The United States Of America As Represented By The United States Department Of Energy Micro-valve pump light valve display
DE4234086A1 (de) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
US5486337A (en) * 1994-02-18 1996-01-23 General Atomics Device for electrostatic manipulation of droplets
US6130098A (en) * 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
DE19717085C2 (de) * 1997-04-23 1999-06-17 Bruker Daltonik Gmbh Verfahren und Geräte für extrem schnelle DNA-Vervielfachung durch Polymerase-Kettenreaktionen (PCR)
US7214298B2 (en) * 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6063339A (en) * 1998-01-09 2000-05-16 Cartesian Technologies, Inc. Method and apparatus for high-speed dot array dispensing
DE59905737D1 (de) 1998-02-11 2003-07-03 Inst Physikalische Hochtech Ev Miniaturisierter temperaturzonen flussreaktor
FI980874A7 (fi) 1998-04-20 1999-10-21 Wallac Oy Menetelmä ja laite pienten nestemäärien kemiallisen analyysin suorittamiseksi
US6565727B1 (en) * 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6326173B1 (en) * 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
IT1309430B1 (it) 1999-05-18 2002-01-23 Guerrieri Roberto Metodo ed apparato per la manipolazione di particelle per mezzo delladielettroforesi
FR2794039B1 (fr) * 1999-05-27 2002-05-03 Osmooze Sa Dispositif de formation, de deplacement et de diffusion de petites quantites calibrees de liquides
AU3986501A (en) * 2000-02-23 2001-09-03 Zyomyx Inc Chips having elevated sample surfaces
US6924792B1 (en) * 2000-03-10 2005-08-02 Richard V. Jessop Electrowetting and electrostatic screen display systems, colour displays and transmission means
AU2001280796A1 (en) 2000-07-25 2002-02-05 The Regents Of The University Of California Electrowetting-driven micropumping
US7465478B2 (en) * 2000-08-11 2008-12-16 Applied Materials, Inc. Plasma immersion ion implantation process
US6773566B2 (en) * 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US7294503B2 (en) * 2000-09-15 2007-11-13 California Institute Of Technology Microfabricated crossflow devices and methods
US7010391B2 (en) * 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
AU2002307152A1 (en) * 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
FR2831081B1 (fr) * 2001-10-24 2004-09-03 Commissariat Energie Atomique Dispositif d'injection parallelisee et synchronisee pour injections sequentielles de reactifs differents
US7338760B2 (en) * 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
US20040231987A1 (en) * 2001-11-26 2004-11-25 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
AU2002359508A1 (en) * 2001-11-26 2003-06-10 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
DE10162188A1 (de) 2001-12-17 2003-06-18 Sunyx Surface Nanotechnologies Hydrophobe Oberfläche mit einer Vielzahl von Elektroden
EP1462517A1 (en) * 2002-01-08 2004-09-29 Japan Science and Technology Agency Pcr method by electrostatic transportation, hybridization method for electrostatic transportation and devices therefor
US7147763B2 (en) * 2002-04-01 2006-12-12 Palo Alto Research Center Incorporated Apparatus and method for using electrostatic force to cause fluid movement
FR2838561B1 (fr) * 2002-04-12 2004-09-17 Commissariat Energie Atomique Matrice de photodectecteurs, a pixels isoles par des murs, hybridee sur un circuit de lecture
FR2841063B1 (fr) * 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
US7130625B2 (en) * 2002-07-01 2006-10-31 3Com Corporation System and method for a universal wireless access gateway
FR2843048B1 (fr) * 2002-08-01 2004-09-24 Commissariat Energie Atomique Dispositif d'injection et de melange de micro-gouttes liquides.
US20040030820A1 (en) * 2002-08-09 2004-02-12 Ching-I Lan Combinational universal serial USB transmission structure
US7329545B2 (en) * 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US6989234B2 (en) * 2002-09-24 2006-01-24 Duke University Method and apparatus for non-contact electrostatic actuation of droplets
US7547380B2 (en) * 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
GB0304033D0 (en) 2003-02-21 2003-03-26 Imp College Innovations Ltd Apparatus
US7041481B2 (en) * 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20050047696A1 (en) * 2003-08-28 2005-03-03 Serrels Dana M. Apparatus and method for retaining bearings
CA2479452C (en) 2003-08-30 2008-11-04 F.Hoffmann-La Roche Ag Method and device for determining analytes in a liquid
DK1695094T3 (da) * 2003-10-24 2013-09-16 Adhesives Res Inc Disintegrerende film til diagnostiske indretninger
ATE434131T1 (de) 2003-11-17 2009-07-15 Koninkl Philips Electronics Nv System zur handhabung einer fluidmenge
CA2553025A1 (en) * 2004-01-14 2005-07-28 Luminex Corporation Methods and systems for dynamic range expansion
FR2866493B1 (fr) * 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
CN2697102Y (zh) * 2004-04-01 2005-05-04 中国人民解放军基因工程研究所 用于pcr扩增仪的液体流动反应恒温箱
FR2872438B1 (fr) 2004-07-01 2006-09-15 Commissariat Energie Atomique Dispositif de deplacement et de traitement de volumes de liquide
US7693666B2 (en) * 2004-07-07 2010-04-06 Rensselaer Polytechnic Institute Method, system, and program product for controlling chemical reactions in a digital microfluidic system
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
WO2006059269A2 (en) * 2004-12-01 2006-06-08 Koninklijke Philips Electronics N.V. Electronic device having logic circuitry and method for designing logic circuitry
DE102004059280B4 (de) * 2004-12-09 2007-08-16 Dräger Safety AG & Co. KGaA Elektrochemischer Gassensor
FR2879946B1 (fr) 2004-12-23 2007-02-09 Commissariat Energie Atomique Dispositif de dispense de gouttes
US7458661B2 (en) * 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
DK1859330T3 (da) 2005-01-28 2012-10-15 Univ Duke Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort
FR2884437B1 (fr) 2005-04-19 2007-07-20 Commissariat Energie Atomique Dispositif et procede microfluidique de transfert de matiere entre deux phases immiscibles.
US9517469B2 (en) 2005-05-11 2016-12-13 Advanced Liquid Logic, Inc. Method and device for conducting biochemical or chemical reactions at multiple temperatures
JP4547301B2 (ja) * 2005-05-13 2010-09-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス及び分析システム
EP1919618A2 (en) 2005-05-21 2008-05-14 Core-Microsolutions, Inc. Mitigation of biomolecular adsorption with hydrophilic polymer additives
JP4500733B2 (ja) 2005-05-30 2010-07-14 株式会社日立ハイテクノロジーズ 化学分析装置
JP2006329904A (ja) 2005-05-30 2006-12-07 Hitachi High-Technologies Corp 液体搬送デバイス及び分析システム
EP1890815A1 (en) 2005-06-16 2008-02-27 Core-Microsolutions, Inc. Biosensor detection by means of droplet driving, agitation, and evaporation
EP1899048B1 (fr) 2005-07-01 2008-12-17 Commissariat A L'energie Atomique Revetement de surface hydrophobe et a faible hysteresis de mouillage, procede de depot d'un tel revetement, micro-composant et utilisation
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
CN101268355A (zh) 2005-09-21 2008-09-17 卢米尼克斯股份有限公司 图像数据处理的方法和系统
US7344679B2 (en) 2005-10-14 2008-03-18 International Business Machines Corporation Method and apparatus for point of care osmolarity testing
US8304253B2 (en) 2005-10-22 2012-11-06 Advanced Liquid Logic Inc Droplet extraction from a liquid column for on-chip microfluidics
WO2007103859A2 (en) 2006-03-03 2007-09-13 Luminex Corporation Methods, products, and kits for identifying an analyte in a sample
US8492168B2 (en) 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
US8637317B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
US8613889B2 (en) 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
US7815871B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
US8470606B2 (en) 2006-04-18 2013-06-25 Duke University Manipulation of beads in droplets and methods for splitting droplets
US8685754B2 (en) 2006-04-18 2014-04-01 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for immunoassays and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
WO2007123908A2 (en) 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US7816121B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7851184B2 (en) 2006-04-18 2010-12-14 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification method and apparatus
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US7763471B2 (en) 2006-04-18 2010-07-27 Advanced Liquid Logic, Inc. Method of electrowetting droplet operations for protein crystallization
CA2680061C (en) 2006-04-18 2015-10-13 Duke University Droplet-based biochemistry
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
WO2008051310A2 (en) 2006-05-09 2008-05-02 Advanced Liquid Logic, Inc. Droplet manipulation systems
US7822510B2 (en) 2006-05-09 2010-10-26 Advanced Liquid Logic, Inc. Systems, methods, and products for graphically illustrating and controlling a droplet actuator
US8041463B2 (en) 2006-05-09 2011-10-18 Advanced Liquid Logic, Inc. Modular droplet actuator drive
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US9266076B2 (en) 2006-11-02 2016-02-23 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
US8338166B2 (en) * 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
US8093062B2 (en) 2007-03-22 2012-01-10 Theodore Winger Enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil
JP5592355B2 (ja) 2008-05-13 2014-09-17 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴アクチュエータ装置、システム、および方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030082081A1 (en) * 2001-10-24 2003-05-01 Commissariat A L'energie Atomique Device for parallel and synchronous injection for sequential injection of different reagents
EP1510254A2 (de) * 2003-08-30 2005-03-02 Roche Diagnostics GmbH Verfahren und Vorrichtung zur Bestimmung von Analyten in einer Flüssigkeit

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VINET, F. et al., Microelectronic Engineering, 2002, vol. 61-62, pages 41-47 *

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