AU2001267752B2 - Improved capture and detection of target nucleic acid in dipstick assays - Google Patents
Improved capture and detection of target nucleic acid in dipstick assays Download PDFInfo
- Publication number
- AU2001267752B2 AU2001267752B2 AU2001267752A AU2001267752A AU2001267752B2 AU 2001267752 B2 AU2001267752 B2 AU 2001267752B2 AU 2001267752 A AU2001267752 A AU 2001267752A AU 2001267752 A AU2001267752 A AU 2001267752A AU 2001267752 B2 AU2001267752 B2 AU 2001267752B2
- Authority
- AU
- Australia
- Prior art keywords
- probe
- nucleic acid
- capture
- target nucleic
- sample solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 414
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 413
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 410
- 238000001514 detection method Methods 0.000 title claims abstract description 313
- 238000003556 assay Methods 0.000 title claims abstract description 24
- 239000000523 sample Substances 0.000 claims abstract description 704
- 239000012488 sample solution Substances 0.000 claims abstract description 188
- 230000027455 binding Effects 0.000 claims abstract description 37
- 238000012360 testing method Methods 0.000 claims abstract description 24
- 238000009396 hybridization Methods 0.000 claims description 114
- 238000000034 method Methods 0.000 claims description 57
- 230000002708 enhancing effect Effects 0.000 claims description 39
- 239000002773 nucleotide Substances 0.000 claims description 19
- 125000003729 nucleotide group Chemical group 0.000 claims description 19
- 239000002253 acid Substances 0.000 claims description 10
- 101100313377 Caenorhabditis elegans stip-1 gene Proteins 0.000 claims 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims 1
- 101100313382 Dictyostelium discoideum stip-2 gene Proteins 0.000 claims 1
- 101100516335 Rattus norvegicus Necab1 gene Proteins 0.000 claims 1
- 101150059016 TFIP11 gene Proteins 0.000 claims 1
- 235000012308 Tagetes Nutrition 0.000 claims 1
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- 230000035945 sensitivity Effects 0.000 abstract description 27
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 34
- 239000003446 ligand Substances 0.000 description 28
- 108020004414 DNA Proteins 0.000 description 25
- 241000606153 Chlamydia trachomatis Species 0.000 description 21
- 230000001745 anti-biotin effect Effects 0.000 description 21
- 229940038705 chlamydia trachomatis Drugs 0.000 description 21
- 239000000975 dye Substances 0.000 description 19
- 229960002685 biotin Drugs 0.000 description 17
- 235000020958 biotin Nutrition 0.000 description 17
- 239000011616 biotin Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 10
- 239000000020 Nitrocellulose Substances 0.000 description 9
- 229920001220 nitrocellulos Polymers 0.000 description 9
- 230000003993 interaction Effects 0.000 description 8
- 102000053602 DNA Human genes 0.000 description 5
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 5
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
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- 238000006243 chemical reaction Methods 0.000 description 4
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- 239000013612 plasmid Substances 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 108090001008 Avidin Proteins 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
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- UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
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- 238000003199 nucleic acid amplification method Methods 0.000 description 2
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- 235000008476 powdered milk Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
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- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 208000019802 Sexually transmitted disease Diseases 0.000 description 1
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 1
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
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- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
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- 239000004753 textile Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/94—Development
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Farming Of Fish And Shellfish (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0016814.6A GB0016814D0 (en) | 2000-07-07 | 2000-07-07 | Improved dipstick assays (3) |
| GB0016814.6 | 2000-07-07 | ||
| PCT/GB2001/003024 WO2002004668A2 (en) | 2000-07-07 | 2001-07-06 | Improved capture and detection of target nucleic acid in dipstick assays |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2001267752A1 AU2001267752A1 (en) | 2002-04-18 |
| AU2001267752B2 true AU2001267752B2 (en) | 2007-05-24 |
Family
ID=9895291
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001267752A Ceased AU2001267752B2 (en) | 2000-07-07 | 2001-07-06 | Improved capture and detection of target nucleic acid in dipstick assays |
| AU6775201A Pending AU6775201A (en) | 2000-07-07 | 2001-07-06 | Improved capture and detection of target nucleic acid in dipstick assays |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU6775201A Pending AU6775201A (en) | 2000-07-07 | 2001-07-06 | Improved capture and detection of target nucleic acid in dipstick assays |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US7186508B2 (enExample) |
| EP (1) | EP1301628B1 (enExample) |
| JP (1) | JP2004502464A (enExample) |
| CN (1) | CN1498275A (enExample) |
| AT (1) | ATE486137T1 (enExample) |
| AU (2) | AU2001267752B2 (enExample) |
| DE (1) | DE60143355D1 (enExample) |
| GB (1) | GB0016814D0 (enExample) |
| TW (1) | TWI281501B (enExample) |
| WO (1) | WO2002004668A2 (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0016833D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (2) |
| GB0016814D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (3) |
| GB0016813D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (4) |
| GB0016836D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (1) |
| GB0025245D0 (en) * | 2000-10-14 | 2000-11-29 | Lee Helen | Multiple target detection |
| US20090047673A1 (en) * | 2006-08-22 | 2009-02-19 | Cary Robert B | Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
| US8980561B1 (en) | 2006-08-22 | 2015-03-17 | Los Alamos National Security, Llc. | Nucleic acid detection system and method for detecting influenza |
| GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
| WO2009137059A1 (en) | 2008-05-05 | 2009-11-12 | Los Alamos National Security, Llc | Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control |
| US20110160090A1 (en) * | 2008-05-05 | 2011-06-30 | Los Alamos National Laboratory | Nanocrystal-Based Lateral Flow Microarrays and Low-Voltage Signal Detection Systems |
| WO2012070618A1 (ja) | 2010-11-24 | 2012-05-31 | 株式会社カネカ | 増幅核酸検出方法及び検出デバイス |
| CA2856304C (en) | 2011-04-20 | 2017-05-16 | Mesa Tech International, Inc. | Oscillating amplification reaction for nucleic acids |
| WO2013129457A1 (ja) * | 2012-02-27 | 2013-09-06 | 東レ株式会社 | 核酸の検出方法 |
| US9783844B2 (en) * | 2012-04-27 | 2017-10-10 | Kaneka Corporation | Method for amplifying nucleic acid and method for detecting amplified nucleic acid |
| WO2015076356A1 (ja) | 2013-11-22 | 2015-05-28 | 株式会社カネカ | 短鎖ｒｎａの検出方法 |
| WO2015119035A1 (ja) * | 2014-02-05 | 2015-08-13 | 扶桑薬品工業株式会社 | マスクオリゴヌクレオチドを用いた核酸の検出、定量方法及びそのデバイス |
| WO2016170181A1 (en) * | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for hybridizing a nucleic acid molecule |
| JP6830443B2 (ja) | 2015-04-24 | 2021-02-17 | メサ バイオテック，インク． | 流体検査用カセット |
| WO2016170182A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
| WO2016170179A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
| CN108026581A (zh) | 2015-06-19 | 2018-05-11 | 剑桥企业有限公司 | 感染性疾病的诊断与治疗 |
| WO2022067023A1 (en) * | 2020-09-24 | 2022-03-31 | Mayo Foundation For Medical Education And Research | Screening platforms |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0318245A2 (en) * | 1987-11-24 | 1989-05-31 | Gen-Probe Incorporated | Means and method for enhancing nucleic acid hybridization |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4358535A (en) | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
| FI843529A0 (fi) | 1983-01-10 | 1984-09-10 | Gen Probe Inc | Foerfarande foer detektering, identifiering och kvantitering av organismer och virus. |
| GB8509367D0 (en) * | 1985-04-12 | 1985-05-15 | Amersham Int Plc | Nucleic acid hybridisation |
| US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| US5310650A (en) * | 1986-09-29 | 1994-05-10 | Abbott Laboratoires | Method and device for improved reaction kinetics in nucleic acid hybridizations |
| IT1224253B (it) | 1988-04-08 | 1990-09-26 | Sclavo Spa | Oligonucleotidi sintetici utili per la determinazione di chlamydia trachomatis in un campione biologico |
| US5217862A (en) * | 1989-05-24 | 1993-06-08 | Amoco Corporation | Method for the detection of Neisseria gonorrhoeae |
| US5130209A (en) | 1989-11-09 | 1992-07-14 | Allied-Signal Inc. | Arc sprayed continuously reinforced aluminum base composites and method |
| IT1244706B (it) | 1991-02-07 | 1994-08-08 | Sclavo Spa | Plasmide pctd isolato da chlamydia trachomatis serotipo d, suoi geni e proteine da essi codificate; plasmidi ricombinanti per l'espressionedi detti geni in sistemi eterologhi come proteine ricombinanti di fusione, preparazione di dette proteine ricombinanti e loro uso nella formulazione di vaccini e/o diagnostici |
| EP0705426A4 (en) * | 1993-06-09 | 1998-07-08 | Quidel Corp | SPECIFIC ONE-STEP TITRATIONS OF AN ANTIGEN |
| US5681697A (en) * | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| WO1995027081A1 (en) | 1994-03-31 | 1995-10-12 | E.I. Du Pont De Nemours And Company | A method for detection of nucleic acid fragments |
| US5656427A (en) | 1994-08-29 | 1997-08-12 | Gen-Probe Incorporated | Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid |
| US5514551A (en) | 1994-10-14 | 1996-05-07 | Gen-Probe Incorporated | Compositions for the detection of Chlamydia trachomatis |
| US5747248A (en) * | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| US5622827A (en) * | 1995-02-07 | 1997-04-22 | Gen-Probe Incorporated | Amplification primers and nucleic acid probes targeted to coccidioides immitis nucleic acid |
| US20020197614A1 (en) * | 1997-05-16 | 2002-12-26 | Mosaic Technologies, Inc. | Electrophoretic analysis of target molecules using adapter molecules |
| US6232067B1 (en) | 1998-08-17 | 2001-05-15 | The Perkin-Elmer Corporation | Adapter directed expression analysis |
| US6821770B1 (en) * | 1999-05-03 | 2004-11-23 | Gen-Probe Incorporated | Polynucleotide matrix-based method of identifying microorganisms |
| GB0016814D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (3) |
-
2000
- 2000-07-07 GB GBGB0016814.6A patent/GB0016814D0/en not_active Ceased
-
2001
- 2001-07-06 AT AT01945538T patent/ATE486137T1/de not_active IP Right Cessation
- 2001-07-06 AU AU2001267752A patent/AU2001267752B2/en not_active Ceased
- 2001-07-06 US US10/332,134 patent/US7186508B2/en not_active Expired - Lifetime
- 2001-07-06 DE DE60143355T patent/DE60143355D1/de not_active Expired - Lifetime
- 2001-07-06 EP EP01945538A patent/EP1301628B1/en not_active Expired - Lifetime
- 2001-07-06 TW TW090116569A patent/TWI281501B/zh not_active IP Right Cessation
- 2001-07-06 WO PCT/GB2001/003024 patent/WO2002004668A2/en not_active Ceased
- 2001-07-06 AU AU6775201A patent/AU6775201A/xx active Pending
- 2001-07-06 JP JP2002509521A patent/JP2004502464A/ja active Pending
- 2001-07-06 CN CNA018153054A patent/CN1498275A/zh active Pending
-
2007
- 2007-01-19 US US11/624,861 patent/US7867706B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0318245A2 (en) * | 1987-11-24 | 1989-05-31 | Gen-Probe Incorporated | Means and method for enhancing nucleic acid hybridization |
Also Published As
| Publication number | Publication date |
|---|---|
| AU6775201A (en) | 2002-01-21 |
| GB0016814D0 (en) | 2000-08-30 |
| ATE486137T1 (de) | 2010-11-15 |
| US7867706B2 (en) | 2011-01-11 |
| US20070190548A1 (en) | 2007-08-16 |
| EP1301628A2 (en) | 2003-04-16 |
| US20060094005A1 (en) | 2006-05-04 |
| TWI281501B (en) | 2007-05-21 |
| CN1498275A (zh) | 2004-05-19 |
| JP2004502464A (ja) | 2004-01-29 |
| EP1301628B1 (en) | 2010-10-27 |
| WO2002004668A2 (en) | 2002-01-17 |
| WO2002004668A3 (en) | 2003-02-06 |
| US7186508B2 (en) | 2007-03-06 |
| DE60143355D1 (de) | 2010-12-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC1 | Assignment before grant (sect. 113) |
Owner name: DIAGNOSTICS FOR THE REAL WORLD, LTD. Free format text: FORMER APPLICANT(S): LEE, HELEN |
|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |