AU2001237178A1 - Endometriosis-related markers and uses thereof - Google Patents
Endometriosis-related markers and uses thereofInfo
- Publication number
- AU2001237178A1 AU2001237178A1 AU2001237178A AU3717801A AU2001237178A1 AU 2001237178 A1 AU2001237178 A1 AU 2001237178A1 AU 2001237178 A AU2001237178 A AU 2001237178A AU 3717801 A AU3717801 A AU 3717801A AU 2001237178 A1 AU2001237178 A1 AU 2001237178A1
- Authority
- AU
- Australia
- Prior art keywords
- endometriosis
- group
- seq
- cdnas
- genes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 201000009273 Endometriosis Diseases 0.000 title claims abstract description 345
- 238000000034 method Methods 0.000 claims abstract description 142
- 210000005168 endometrial cell Anatomy 0.000 claims abstract description 92
- 239000000523 sample Substances 0.000 claims abstract description 48
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 16
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 16
- 239000002157 polynucleotide Substances 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 248
- 230000014509 gene expression Effects 0.000 claims description 169
- 239000003550 marker Substances 0.000 claims description 149
- 229920002477 rna polymer Polymers 0.000 claims description 121
- 108020004635 Complementary DNA Proteins 0.000 claims description 105
- 150000007523 nucleic acids Chemical class 0.000 claims description 91
- 102000004169 proteins and genes Human genes 0.000 claims description 87
- 102000039446 nucleic acids Human genes 0.000 claims description 86
- 108020004707 nucleic acids Proteins 0.000 claims description 86
- 239000012634 fragment Substances 0.000 claims description 49
- -1 hUCC1 Proteins 0.000 claims description 48
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 46
- 239000002299 complementary DNA Substances 0.000 claims description 36
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 32
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 31
- 230000003248 secreting effect Effects 0.000 claims description 31
- 230000027758 ovulation cycle Effects 0.000 claims description 28
- 108010069241 Connexin 43 Proteins 0.000 claims description 26
- 210000004027 cell Anatomy 0.000 claims description 26
- 239000012528 membrane Substances 0.000 claims description 25
- 230000035752 proliferative phase Effects 0.000 claims description 25
- 108700036248 MT-RNR1 Proteins 0.000 claims description 24
- 102000016938 Catalase Human genes 0.000 claims description 21
- 108010053835 Catalase Proteins 0.000 claims description 21
- 102000009836 Aconitate hydratase Human genes 0.000 claims description 20
- 108010009924 Aconitate hydratase Proteins 0.000 claims description 20
- 101150071146 COX2 gene Proteins 0.000 claims description 20
- 101001050288 Homo sapiens Transcription factor Jun Proteins 0.000 claims description 19
- 102100023132 Transcription factor Jun Human genes 0.000 claims description 19
- 230000010627 oxidative phosphorylation Effects 0.000 claims description 19
- 102100032533 ADP/ATP translocase 1 Human genes 0.000 claims description 18
- 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 claims description 18
- 101000971171 Homo sapiens Apoptosis regulator Bcl-2 Proteins 0.000 claims description 18
- 230000027455 binding Effects 0.000 claims description 18
- 101710148586 ADP,ATP carrier protein 1 Proteins 0.000 claims description 17
- 101710111394 ADP,ATP carrier protein 1, mitochondrial Proteins 0.000 claims description 17
- 101710102716 ADP/ATP translocase 1 Proteins 0.000 claims description 17
- 102100032510 Heat shock protein HSP 90-beta Human genes 0.000 claims description 17
- 101001016856 Homo sapiens Heat shock protein HSP 90-beta Proteins 0.000 claims description 17
- 101000988090 Leishmania donovani Heat shock protein 83 Proteins 0.000 claims description 17
- LPEPZBJOKDYZAD-UHFFFAOYSA-N flufenamic acid Chemical compound OC(=O)C1=CC=CC=C1NC1=CC=CC(C(F)(F)F)=C1 LPEPZBJOKDYZAD-UHFFFAOYSA-N 0.000 claims description 17
- 102100030907 Aryl hydrocarbon receptor nuclear translocator Human genes 0.000 claims description 16
- 102000006746 NADH Dehydrogenase Human genes 0.000 claims description 16
- 108010086428 NADH Dehydrogenase Proteins 0.000 claims description 16
- 102000003777 Interleukin-1 beta Human genes 0.000 claims description 15
- 108090000193 Interleukin-1 beta Proteins 0.000 claims description 15
- 101000839464 Leishmania braziliensis Heat shock 70 kDa protein Proteins 0.000 claims description 15
- 108090000944 RNA Helicases Proteins 0.000 claims description 15
- 102000004409 RNA Helicases Human genes 0.000 claims description 15
- 230000037361 pathway Effects 0.000 claims description 13
- 101710128947 Tricarboxylate transport protein, mitochondrial Proteins 0.000 claims description 12
- 102100036216 Tricarboxylate transport protein, mitochondrial Human genes 0.000 claims description 12
- 238000009396 hybridization Methods 0.000 claims description 12
- 230000002018 overexpression Effects 0.000 claims description 12
- 108020004414 DNA Proteins 0.000 claims description 11
- 108700041152 Endoplasmic Reticulum Chaperone BiP Proteins 0.000 claims description 11
- 102100021451 Endoplasmic reticulum chaperone BiP Human genes 0.000 claims description 11
- 101150112743 HSPA5 gene Proteins 0.000 claims description 11
- 101100111629 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) KAR2 gene Proteins 0.000 claims description 11
- 101150028578 grp78 gene Proteins 0.000 claims description 11
- 238000010839 reverse transcription Methods 0.000 claims description 11
- 230000009452 underexpressoin Effects 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 102000043924 Paralemmin Human genes 0.000 claims description 10
- 108700038311 Paralemmin Proteins 0.000 claims description 10
- 238000002372 labelling Methods 0.000 claims description 10
- 238000003757 reverse transcription PCR Methods 0.000 claims description 10
- 108091060211 Expressed sequence tag Proteins 0.000 claims description 9
- 238000013518 transcription Methods 0.000 claims description 9
- 230000035897 transcription Effects 0.000 claims description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
- 238000000018 DNA microarray Methods 0.000 claims description 7
- 238000000338 in vitro Methods 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 6
- 238000003364 immunohistochemistry Methods 0.000 claims description 6
- 108020004999 messenger RNA Proteins 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- 238000007901 in situ hybridization Methods 0.000 claims description 5
- 238000002965 ELISA Methods 0.000 claims description 4
- 238000002405 diagnostic procedure Methods 0.000 claims description 4
- 238000000684 flow cytometry Methods 0.000 claims description 4
- 238000003365 immunocytochemistry Methods 0.000 claims description 4
- 238000003786 synthesis reaction Methods 0.000 claims description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 3
- 238000010562 histological examination Methods 0.000 claims description 3
- 150000003431 steroids Chemical class 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 230000009149 molecular binding Effects 0.000 claims description 2
- 102100033512 GTP:AMP phosphotransferase AK3, mitochondrial Human genes 0.000 claims 8
- 101000614487 Homo sapiens Adenylate kinase 4, mitochondrial Proteins 0.000 claims 8
- 101000998053 Homo sapiens GTP:AMP phosphotransferase AK3, mitochondrial Proteins 0.000 claims 8
- 102000001045 Connexin 43 Human genes 0.000 claims 7
- 101000617823 Homo sapiens Solute carrier organic anion transporter family member 6A1 Proteins 0.000 claims 7
- 102100021991 Solute carrier organic anion transporter family member 6A1 Human genes 0.000 claims 7
- 101710119418 Superoxide dismutase [Mn] Proteins 0.000 claims 6
- 101710202572 Superoxide dismutase [Mn], mitochondrial Proteins 0.000 claims 6
- 102100032891 Superoxide dismutase [Mn], mitochondrial Human genes 0.000 claims 6
- 101000690445 Caenorhabditis elegans Aryl hydrocarbon receptor nuclear translocator homolog Proteins 0.000 claims 5
- 102100035427 Forkhead box protein O1 Human genes 0.000 claims 5
- 101000793115 Homo sapiens Aryl hydrocarbon receptor nuclear translocator Proteins 0.000 claims 5
- 101000877727 Homo sapiens Forkhead box protein O1 Proteins 0.000 claims 5
- 108091008747 NR2F3 Proteins 0.000 claims 5
- 102100028452 Nitric oxide synthase, endothelial Human genes 0.000 claims 4
- 101710090055 Nitric oxide synthase, endothelial Proteins 0.000 claims 4
- 101001009581 Molluscum contagiosum virus subtype 1 Glutathione peroxidase Proteins 0.000 claims 2
- 101001014955 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Glutathione peroxidase-like peroxiredoxin gpx1 Proteins 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 35
- 201000010099 disease Diseases 0.000 abstract description 34
- 239000013615 primer Substances 0.000 abstract description 13
- 238000002357 laparoscopic surgery Methods 0.000 abstract description 7
- 239000002987 primer (paints) Substances 0.000 abstract description 6
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 90
- 230000035945 sensitivity Effects 0.000 description 78
- 235000018102 proteins Nutrition 0.000 description 66
- 239000000047 product Substances 0.000 description 45
- 238000001574 biopsy Methods 0.000 description 39
- 210000001519 tissue Anatomy 0.000 description 25
- 102000019197 Superoxide Dismutase Human genes 0.000 description 20
- 108010012715 Superoxide dismutase Proteins 0.000 description 20
- 230000007423 decrease Effects 0.000 description 20
- 102100021337 Gap junction alpha-1 protein Human genes 0.000 description 19
- 230000002068 genetic effect Effects 0.000 description 19
- 210000004379 membrane Anatomy 0.000 description 19
- 210000004696 endometrium Anatomy 0.000 description 17
- 230000002357 endometrial effect Effects 0.000 description 16
- 230000000762 glandular Effects 0.000 description 15
- 102000006587 Glutathione peroxidase Human genes 0.000 description 13
- 108700016172 Glutathione peroxidases Proteins 0.000 description 13
- 108010049386 Aryl Hydrocarbon Receptor Nuclear Translocator Proteins 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 12
- 102000008052 Nitric Oxide Synthase Type III Human genes 0.000 description 10
- 108010075520 Nitric Oxide Synthase Type III Proteins 0.000 description 10
- 238000013459 approach Methods 0.000 description 10
- 230000003247 decreasing effect Effects 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 210000003470 mitochondria Anatomy 0.000 description 8
- 102100032742 Histone-lysine N-methyltransferase SETD2 Human genes 0.000 description 7
- 101000654725 Homo sapiens Histone-lysine N-methyltransferase SETD2 Proteins 0.000 description 7
- 238000000636 Northern blotting Methods 0.000 description 7
- 102100028262 U6 snRNA-associated Sm-like protein LSm4 Human genes 0.000 description 7
- 230000002438 mitochondrial effect Effects 0.000 description 7
- 239000003642 reactive oxygen metabolite Substances 0.000 description 7
- 230000001105 regulatory effect Effects 0.000 description 7
- 230000003827 upregulation Effects 0.000 description 7
- 206010021143 Hypoxia Diseases 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 6
- 230000002611 ovarian Effects 0.000 description 6
- 238000007619 statistical method Methods 0.000 description 6
- 102100038280 Prostaglandin G/H synthase 2 Human genes 0.000 description 5
- 230000018199 S phase Effects 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 5
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 5
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 5
- 230000007954 hypoxia Effects 0.000 description 5
- 238000002513 implantation Methods 0.000 description 5
- 208000000509 infertility Diseases 0.000 description 5
- 230000036512 infertility Effects 0.000 description 5
- 230000003902 lesion Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000002773 nucleotide Chemical group 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 230000002062 proliferating effect Effects 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 101100116283 Arabidopsis thaliana DD11 gene Proteins 0.000 description 4
- 102000014654 Aromatase Human genes 0.000 description 4
- 108010078554 Aromatase Proteins 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 108010037462 Cyclooxygenase 2 Proteins 0.000 description 4
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 4
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 4
- 102100034343 Integrase Human genes 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 231100000535 infertility Toxicity 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000036542 oxidative stress Effects 0.000 description 4
- 230000001850 reproductive effect Effects 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 210000002536 stromal cell Anatomy 0.000 description 4
- 108091023043 Alu Element Proteins 0.000 description 3
- 102000010970 Connexin Human genes 0.000 description 3
- 108050001175 Connexin Proteins 0.000 description 3
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 3
- 108050008072 Cytochrome c oxidase subunit IV Proteins 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 101001046870 Homo sapiens Hypoxia-inducible factor 1-alpha Proteins 0.000 description 3
- 102100022875 Hypoxia-inducible factor 1-alpha Human genes 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 108091036066 Three prime untranslated region Proteins 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000002596 correlated effect Effects 0.000 description 3
- 238000001784 detoxification Methods 0.000 description 3
- 230000027721 electron transport chain Effects 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 238000010195 expression analysis Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000003054 hormonal effect Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000002350 laparotomy Methods 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 150000003180 prostaglandins Chemical class 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 238000010200 validation analysis Methods 0.000 description 3
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 108091006112 ATPases Proteins 0.000 description 2
- 208000005641 Adenomyosis Diseases 0.000 description 2
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 2
- 102100032534 Adenosine kinase Human genes 0.000 description 2
- 108020000543 Adenylate kinase Proteins 0.000 description 2
- 101710163595 Chaperone protein DnaK Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 239000003155 DNA primer Substances 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 102000005720 Glutathione transferase Human genes 0.000 description 2
- 108010070675 Glutathione transferase Proteins 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 description 2
- 101710152018 Heat shock cognate 70 kDa protein Proteins 0.000 description 2
- 101000605127 Homo sapiens Prostaglandin G/H synthase 2 Proteins 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 description 2
- 208000037093 Menstruation Disturbances Diseases 0.000 description 2
- 108020005196 Mitochondrial DNA Proteins 0.000 description 2
- HRNLUBSXIHFDHP-UHFFFAOYSA-N N-(2-aminophenyl)-4-[[[4-(3-pyridinyl)-2-pyrimidinyl]amino]methyl]benzamide Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC1=NC=CC(C=2C=NC=CC=2)=N1 HRNLUBSXIHFDHP-UHFFFAOYSA-N 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 101150006573 PAN1 gene Proteins 0.000 description 2
- 208000000450 Pelvic Pain Diseases 0.000 description 2
- 108010033024 Phospholipid Hydroperoxide Glutathione Peroxidase Proteins 0.000 description 2
- 102100023410 Phospholipid hydroperoxide glutathione peroxidase Human genes 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 239000013614 RNA sample Substances 0.000 description 2
- 206010065951 Retrograde menstruation Diseases 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- 230000001594 aberrant effect Effects 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 108091092328 cellular RNA Proteins 0.000 description 2
- 230000004637 cellular stress Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 208000029742 colonic neoplasm Diseases 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000003828 downregulation Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 201000009274 endometriosis of uterus Diseases 0.000 description 2
- 230000006862 enzymatic digestion Effects 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 229960005309 estradiol Drugs 0.000 description 2
- 229930182833 estradiol Natural products 0.000 description 2
- 239000000262 estrogen Substances 0.000 description 2
- 229940011871 estrogen Drugs 0.000 description 2
- 210000003976 gap junction Anatomy 0.000 description 2
- 238000001415 gene therapy Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000001608 morphoregulatory effect Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000004792 oxidative damage Effects 0.000 description 2
- 239000013610 patient sample Substances 0.000 description 2
- 210000003200 peritoneal cavity Anatomy 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 208000010543 22q11.2 deletion syndrome Diseases 0.000 description 1
- YPSXFMHXRZAGTG-UHFFFAOYSA-N 4-methoxy-2-[2-(5-methoxy-2-nitrosophenyl)ethyl]-1-nitrosobenzene Chemical compound COC1=CC=C(N=O)C(CCC=2C(=CC=C(OC)C=2)N=O)=C1 YPSXFMHXRZAGTG-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 108090001079 Adenine Nucleotide Translocator 1 Proteins 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 108020000948 Antisense Oligonucleotides Proteins 0.000 description 1
- 108010063104 Apoptosis Regulatory Proteins Proteins 0.000 description 1
- 102000010565 Apoptosis Regulatory Proteins Human genes 0.000 description 1
- 108010039627 Aprotinin Proteins 0.000 description 1
- 201000004002 Aromatase excess syndrome Diseases 0.000 description 1
- 208000008035 Back Pain Diseases 0.000 description 1
- 101001111189 Bos taurus NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Proteins 0.000 description 1
- 102100021943 C-C motif chemokine 2 Human genes 0.000 description 1
- 101710155857 C-C motif chemokine 2 Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 208000016216 Choristoma Diseases 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 208000000398 DiGeorge Syndrome Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000005171 Dysmenorrhea Diseases 0.000 description 1
- 206010013935 Dysmenorrhoea Diseases 0.000 description 1
- 208000004483 Dyspareunia Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101150099000 EXPA1 gene Proteins 0.000 description 1
- 206010014733 Endometrial cancer Diseases 0.000 description 1
- 206010014759 Endometrial neoplasm Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 102100029095 Exportin-1 Human genes 0.000 description 1
- 102100029091 Exportin-2 Human genes 0.000 description 1
- 101710147878 Exportin-2 Proteins 0.000 description 1
- 101000609762 Gallus gallus Ovalbumin Proteins 0.000 description 1
- 208000034826 Genetic Predisposition to Disease Diseases 0.000 description 1
- 208000032612 Glial tumor Diseases 0.000 description 1
- 206010018338 Glioma Diseases 0.000 description 1
- 102000042092 Glucose transporter family Human genes 0.000 description 1
- 108091052347 Glucose transporter family Proteins 0.000 description 1
- 206010053240 Glycogen storage disease type VI Diseases 0.000 description 1
- NMJREATYWWNIKX-UHFFFAOYSA-N GnRH Chemical compound C1CCC(C(=O)NCC(N)=O)N1C(=O)C(CC(C)C)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)CNC(=O)C(NC(=O)C(CO)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C(CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 NMJREATYWWNIKX-UHFFFAOYSA-N 0.000 description 1
- 108010027992 HSP70 Heat-Shock Proteins Proteins 0.000 description 1
- 102000018932 HSP70 Heat-Shock Proteins Human genes 0.000 description 1
- 239000012981 Hank's balanced salt solution Substances 0.000 description 1
- 101710113864 Heat shock protein 90 Proteins 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 101000725401 Homo sapiens Cytochrome c oxidase subunit 2 Proteins 0.000 description 1
- 101000979748 Homo sapiens Protein NDRG1 Proteins 0.000 description 1
- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000001974 Hyaluronidases Human genes 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- RHGKLRLOHDJJDR-BYPYZUCNSA-N L-citrulline Chemical compound NC(=O)NCCC[C@H]([NH3+])C([O-])=O RHGKLRLOHDJJDR-BYPYZUCNSA-N 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 102100039364 Metalloproteinase inhibitor 1 Human genes 0.000 description 1
- 108010058682 Mitochondrial Proteins Proteins 0.000 description 1
- 102000006404 Mitochondrial Proteins Human genes 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- RHGKLRLOHDJJDR-UHFFFAOYSA-N Ndelta-carbamoyl-DL-ornithine Natural products OC(=O)C(N)CCCNC(N)=O RHGKLRLOHDJJDR-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 108010089430 Phosphoproteins Proteins 0.000 description 1
- 102000007982 Phosphoproteins Human genes 0.000 description 1
- 102100038124 Plasminogen Human genes 0.000 description 1
- 108010051456 Plasminogen Proteins 0.000 description 1
- 108010076039 Polyproteins Proteins 0.000 description 1
- 208000004965 Prostatic Intraepithelial Neoplasia Diseases 0.000 description 1
- 206010071019 Prostatic dysplasia Diseases 0.000 description 1
- 102100024980 Protein NDRG1 Human genes 0.000 description 1
- 108010071563 Proto-Oncogene Proteins c-fos Proteins 0.000 description 1
- 102000007568 Proto-Oncogene Proteins c-fos Human genes 0.000 description 1
- 108091008109 Pseudogenes Proteins 0.000 description 1
- 102000057361 Pseudogenes Human genes 0.000 description 1
- 101100119348 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) EXP1 gene Proteins 0.000 description 1
- 208000007893 Salpingitis Diseases 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 101000857870 Squalus acanthias Gonadoliberin Proteins 0.000 description 1
- 101100269618 Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) aliA gene Proteins 0.000 description 1
- 101710127774 Stress response protein Proteins 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 108010031374 Tissue Inhibitor of Metalloproteinase-1 Proteins 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 201000008346 acute salpingitis Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000000556 agonist Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- SOIFLUNRINLCBN-UHFFFAOYSA-N ammonium thiocyanate Chemical compound [NH4+].[S-]C#N SOIFLUNRINLCBN-UHFFFAOYSA-N 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000000074 antisense oligonucleotide Substances 0.000 description 1
- 238000012230 antisense oligonucleotides Methods 0.000 description 1
- 229960004405 aprotinin Drugs 0.000 description 1
- 101150024767 arnT gene Proteins 0.000 description 1
- 210000003567 ascitic fluid Anatomy 0.000 description 1
- 238000000211 autoradiogram Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000006567 cellular energy metabolism Effects 0.000 description 1
- 230000008614 cellular interaction Effects 0.000 description 1
- 230000004098 cellular respiration Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 229960002173 citrulline Drugs 0.000 description 1
- 235000013477 citrulline Nutrition 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- POZRVZJJTULAOH-LHZXLZLDSA-N danazol Chemical compound C1[C@]2(C)[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=CC2=C1C=NO2 POZRVZJJTULAOH-LHZXLZLDSA-N 0.000 description 1
- 229960000766 danazol Drugs 0.000 description 1
- 238000000326 densiometry Methods 0.000 description 1
- 230000002074 deregulated effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000008482 dysregulation Effects 0.000 description 1
- 201000003914 endometrial carcinoma Diseases 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 108700002148 exportin 1 Proteins 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000012226 gene silencing method Methods 0.000 description 1
- 238000002695 general anesthesia Methods 0.000 description 1
- 108010017007 glucose-regulated proteins Proteins 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 239000000833 heterodimer Substances 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002432 hydroperoxides Chemical class 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 230000010438 iron metabolism Effects 0.000 description 1
- 108700025907 jun Genes Proteins 0.000 description 1
- 108091008792 l-Myc Proteins 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
- 108010052968 leupeptin Proteins 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000002483 medication Methods 0.000 description 1
- 230000002175 menstrual effect Effects 0.000 description 1
- 230000003821 menstrual periods Effects 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000005787 mitochondrial ATP synthesis coupled electron transport Effects 0.000 description 1
- 208000012268 mitochondrial disease Diseases 0.000 description 1
- 230000008811 mitochondrial respiratory chain Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 108010007425 oligomycin sensitivity conferring protein Proteins 0.000 description 1
- 229940127234 oral contraceptive Drugs 0.000 description 1
- 239000003539 oral contraceptive agent Substances 0.000 description 1
- 230000011599 ovarian follicle development Effects 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000008557 oxygen metabolism Effects 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 201000002094 pancreatic adenocarcinoma Diseases 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000002824 peroxisome Anatomy 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000583 progesterone congener Substances 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 208000021046 prostate intraepithelial neoplasia Diseases 0.000 description 1
- 230000009979 protective mechanism Effects 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 108700022487 rRNA Genes Proteins 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 210000005084 renal tissue Anatomy 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000011506 response to oxidative stress Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000009810 tubal ligation Methods 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Endocrinology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Reproductive Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Radar Systems Or Details Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Orthopedics, Nursing, And Contraception (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18506300P | 2000-02-25 | 2000-02-25 | |
| US60185063 | 2000-02-25 | ||
| US22574500P | 2000-08-17 | 2000-08-17 | |
| US60225745 | 2000-08-17 | ||
| PCT/CA2001/000245 WO2001062959A2 (en) | 2000-02-25 | 2001-02-26 | Endometriosis-related markers and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001237178A1 true AU2001237178A1 (en) | 2001-09-03 |
Family
ID=26880752
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001237178A Abandoned AU2001237178A1 (en) | 2000-02-25 | 2001-02-26 | Endometriosis-related markers and uses thereof |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US6777182B2 (de) |
| EP (1) | EP1290218B8 (de) |
| JP (1) | JP2003531580A (de) |
| AT (1) | ATE365227T1 (de) |
| AU (1) | AU2001237178A1 (de) |
| BR (1) | BR0108685A (de) |
| CA (1) | CA2399259A1 (de) |
| DE (2) | DE60129018T2 (de) |
| ES (2) | ES2336817T3 (de) |
| WO (1) | WO2001062959A2 (de) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100414637B1 (ko) * | 2000-02-08 | 2004-01-13 | (주)에스제이바이오메드 | 사람 미토콘드리아 아데닐레이트 키나제 이소자임들에대한 항체와 면역학적 제제 및 심장질환 진단키트 |
| US6884578B2 (en) * | 2000-03-31 | 2005-04-26 | Affymetrix, Inc. | Genes differentially expressed in secretory versus proliferative endometrium |
| US20060014166A1 (en) * | 2004-01-27 | 2006-01-19 | Yossi Cohen | Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of endometriosis |
| AU2003303576A1 (en) * | 2002-05-14 | 2004-07-29 | The Board Of Trustees Of The Leland Stanford Junior University | Endometrial genes in endometrial disorders |
| US20050214836A1 (en) * | 2002-08-30 | 2005-09-29 | Oncotherapy Science, Inc. | Method of diagnosing ovarian endometriosis |
| US20050100967A1 (en) * | 2003-07-11 | 2005-05-12 | Science & Technology Corporation @ Unm | Detection of endometrial pathology |
| US7268117B2 (en) | 2003-07-11 | 2007-09-11 | Praecis Pharmaceuticals Incorporated | Compositions, kits, and methods for identification, assessment, prevention, and therapy of endometriosis |
| AU2004303448A1 (en) * | 2003-12-23 | 2005-07-07 | Mount Sinai Hospital | Methods for detecting markers associated with endometrial disease or phase |
| US20060008876A1 (en) | 2004-07-07 | 2006-01-12 | Shami A S E | ME-5, ME-2, and EPP2: human protein antigens reactive with autoantibodies present in the serum of women suffering from endometriosis |
| US7943324B2 (en) * | 2004-12-22 | 2011-05-17 | The Trustees Of The University Of Pennsylvania | Endometriosis markers |
| KR20080044836A (ko) | 2005-07-15 | 2008-05-21 | 라보라뚜와르 세로노 에스. 에이. | 자궁내막증 치료용 jnk 억제제 |
| BRPI0613042A2 (pt) * | 2005-07-15 | 2010-12-14 | Serono Lab | inibidores de jnk para o tratamento de endometriose |
| US8338111B2 (en) * | 2006-12-22 | 2012-12-25 | The Trustees Of The University Of Pennsylvania | Endometriosis markers |
| US20080306034A1 (en) * | 2007-06-11 | 2008-12-11 | Juneau Biosciences, Llc | Method of Administering a Therapeutic |
| US11287425B2 (en) | 2009-04-22 | 2022-03-29 | Juneau Biosciences, Llc | Genetic markers associated with endometriosis and use thereof |
| US8932993B1 (en) | 2007-06-11 | 2015-01-13 | Juneau Biosciences, LLC. | Method of testing for endometriosis and treatment therefor |
| US20080305967A1 (en) * | 2007-06-11 | 2008-12-11 | Juneau Biosciences, Llc | Genetic Markers Associated with Endometriosis and Use Thereof |
| KR101666228B1 (ko) | 2007-09-28 | 2016-10-13 | 인트렉손 코포레이션 | 생물치료학적 분자를 발현시키기 위한 치료학적 유전자-스위치 작제물 및 생물반응기, 및 이의 용도 |
| AU2010219820A1 (en) | 2009-03-06 | 2011-09-15 | Mochida Pharmaceutical Co., Ltd. | Method for diagnosing endometriosis and diagnostic kit for endometriosis |
| WO2010107734A2 (en) * | 2009-03-16 | 2010-09-23 | Ozgul Muneyyirci-Delale | Methods for detecting endometriosis |
| GB201012662D0 (en) * | 2010-07-28 | 2010-09-15 | Valirx Plc | Method for detecting the presence of a gynaecological growth |
| DE102012002929A1 (de) | 2012-02-14 | 2013-08-14 | Jürgen Lewald | Minimalinvasives Verfahren für die Diagnose und die Therapieverlaufskontrolle der Endometriose |
| GB201302837D0 (en) * | 2013-02-19 | 2013-04-03 | Univ Singapore | Therapeutic Agents |
| US9434991B2 (en) | 2013-03-07 | 2016-09-06 | Juneau Biosciences, LLC. | Method of testing for endometriosis and treatment therefor |
| SG11201700303UA (en) * | 2014-07-17 | 2017-02-27 | Celmatix Inc | Methods and systems for assessing infertility and related pathologies |
| WO2017167716A1 (en) * | 2016-03-30 | 2017-10-05 | Life Technologies As | Improved methods and compositions for nucleic acid isolation |
| WO2017201748A1 (zh) * | 2016-05-27 | 2017-11-30 | 曾启瑞 | 一种检测子宫内膜异位症之方法与其应用 |
| WO2019115829A1 (en) * | 2017-12-15 | 2019-06-20 | Endodiag Pépinière Génopole Entreprises | Method of diagnosing endometriosis |
| CN112470002A (zh) * | 2018-04-20 | 2021-03-09 | 德克萨斯大学体系董事会 | 用于治疗子宫内膜异位症的组合物和方法 |
| JP7191361B2 (ja) * | 2018-08-13 | 2022-12-19 | 学校法人 埼玉医科大学 | 産婦人科疾患の罹患可能性の判定を補助するための方法、産婦人科疾患の罹患可能性を診断するためのデータを収集する方法、及び産婦人科疾患の診断用キット |
| KR102255691B1 (ko) * | 2018-12-14 | 2021-05-26 | 대한민국 | 돼지 발정주기 탐지용 자궁내막 유전자 및 그의 용도 |
| WO2020218465A1 (ja) * | 2019-04-26 | 2020-10-29 | 中外製薬株式会社 | 子宮内膜症の診断方法、病態モニタリング方法及びキット |
| GB202310300D0 (en) | 2023-07-05 | 2023-08-16 | Univ Edinburgh | Endometrosis markers and methods of diagnosing nedometriosis |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5478725A (en) * | 1992-06-12 | 1995-12-26 | University Of Pennsylvania | αv β3 integrin as a predictor of endometriosis |
| DE19824230A1 (de) * | 1998-05-29 | 1999-12-02 | Starzinski Powitz Anna | Neues Endometriose-assoziiertes Gen |
| CA2299436A1 (en) * | 1998-06-04 | 1999-12-09 | Reprogen, Inc. | Use of prothymosin in the diagnosis and treatment of endometriosis |
| US6387629B1 (en) * | 1998-06-04 | 2002-05-14 | Reprogen, Inc. | Use of cathepsin S in the diagnosis and treatment of endometriosis |
| WO2001063250A1 (en) * | 2000-02-24 | 2001-08-30 | Tokyo Electron Limited | Method and apparatus for leak detecting, and apparatus for semiconductor manufacture |
-
2001
- 2001-02-26 EP EP01909382A patent/EP1290218B8/de not_active Expired - Lifetime
- 2001-02-26 US US09/794,928 patent/US6777182B2/en not_active Expired - Fee Related
- 2001-02-26 CA CA002399259A patent/CA2399259A1/en not_active Abandoned
- 2001-02-26 DE DE60129018T patent/DE60129018T2/de not_active Expired - Lifetime
- 2001-02-26 WO PCT/CA2001/000245 patent/WO2001062959A2/en not_active Ceased
- 2001-02-26 ES ES06023011T patent/ES2336817T3/es not_active Expired - Lifetime
- 2001-02-26 AU AU2001237178A patent/AU2001237178A1/en not_active Abandoned
- 2001-02-26 ES ES01909382T patent/ES2287100T3/es not_active Expired - Lifetime
- 2001-02-26 BR BR0108685-5A patent/BR0108685A/pt not_active Application Discontinuation
- 2001-02-26 DE DE60140580T patent/DE60140580D1/de not_active Expired - Lifetime
- 2001-02-26 AT AT01909382T patent/ATE365227T1/de not_active IP Right Cessation
- 2001-02-26 JP JP2001561767A patent/JP2003531580A/ja active Pending
-
2004
- 2004-08-17 US US10/920,152 patent/US8148061B2/en not_active Expired - Fee Related
-
2012
- 2012-02-23 US US13/403,903 patent/US8541173B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001062959A3 (en) | 2003-01-03 |
| EP1290218A2 (de) | 2003-03-12 |
| BR0108685A (pt) | 2003-12-30 |
| US20020127555A1 (en) | 2002-09-12 |
| WO2001062959A2 (en) | 2001-08-30 |
| ATE365227T1 (de) | 2007-07-15 |
| US8541173B2 (en) | 2013-09-24 |
| US20060057584A1 (en) | 2006-03-16 |
| DE60129018T2 (de) | 2008-02-21 |
| US8148061B2 (en) | 2012-04-03 |
| ES2287100T3 (es) | 2007-12-16 |
| EP1290218B1 (de) | 2007-06-20 |
| JP2003531580A (ja) | 2003-10-28 |
| US6777182B2 (en) | 2004-08-17 |
| US20120208869A1 (en) | 2012-08-16 |
| EP1290218B8 (de) | 2007-10-03 |
| ES2336817T3 (es) | 2010-04-16 |
| DE60140580D1 (de) | 2009-12-31 |
| DE60129018D1 (de) | 2007-08-02 |
| CA2399259A1 (en) | 2001-08-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8541173B2 (en) | Endometriosis-related markers and uses thereof | |
| EP2848700B1 (de) | Marker für endometriosetumor | |
| EP1537243B1 (de) | Verfahren zur diagnostik von eierstock endometriose | |
| CN109666743B (zh) | 一种宫颈癌分子标志物及其应用 | |
| US20090087843A1 (en) | Molecular markers | |
| EP1484399A1 (de) | Maus-spermatogenesegene, menschliche mit männlicher sterilität assoziierte gene und diese verwendendes diagnosesystem | |
| EP1801236B1 (de) | Endometriose Marker und Verwendung derselben | |
| EP1682679B1 (de) | Molekularer marker | |
| CN114107461B (zh) | 环状rna标志物及其在诊断和预测复发性流产病理妊娠中的应用 | |
| EP1354060B1 (de) | 5t4 rna in plasma und serum als marker für neoplastische erkrankungen | |
| RU2848546C1 (ru) | Набор олигонуклеотидов для генотипирования полиморфных маркеров хронического эндометрита rs3020450 гена ESR2 в клиническом материале методом полимеразной цепной реакции с детекцией результатов амплификации в режиме реального времени | |
| EP1660676B1 (de) | Diagnose des brustkrebsrisikos | |
| CN114107462B (zh) | 流产标志物及其在诊断和预测复发性流产病理妊娠中的应用 | |
| CN110144352B (zh) | 用于早期诊断骨科疾病的分子标志物 | |
| Gylfason et al. | Quantitative DNA perturbations of p53 in endometriosis: analysis of American and Icelandic cases | |
| KR20180137049A (ko) | 삼중음성 유방암 특이적 단백질분해조절 효소 유전자를 포함하는 삼중음성 유방암 바이오마커 및 이를 이용한 삼중음성 유방암 진단을 위한 정보제공방법 | |
| EP1646728A1 (de) | Gentischer marker für die koronare herzkrankheit | |
| EP1932926A2 (de) | Verfahren zur Diagnostik von Eierstock Endometriose | |
| WO2004031386A1 (ja) | アトピー性皮膚炎の検査方法 | |
| WO2005012564A1 (en) | Genetic marker for coronary artery disease |