ATE486136T1 - Verwendung von ammonium- und phosphationen zur verbesserung des nachweises von luciferase - Google Patents
Verwendung von ammonium- und phosphationen zur verbesserung des nachweises von luciferaseInfo
- Publication number
- ATE486136T1 ATE486136T1 AT06733050T AT06733050T ATE486136T1 AT E486136 T1 ATE486136 T1 AT E486136T1 AT 06733050 T AT06733050 T AT 06733050T AT 06733050 T AT06733050 T AT 06733050T AT E486136 T1 ATE486136 T1 AT E486136T1
- Authority
- AT
- Austria
- Prior art keywords
- luciferase
- relates
- light signal
- detection
- ammonium
- Prior art date
Links
- 108060001084 Luciferase Proteins 0.000 title abstract 4
- 239000005089 Luciferase Substances 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 abstract 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 abstract 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 abstract 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- -1 ammonium ions Chemical class 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 230000029918 bioluminescence Effects 0.000 abstract 1
- 238000005415 bioluminescence Methods 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68109305P | 2005-05-13 | 2005-05-13 | |
| EP05076165A EP1724359A1 (de) | 2005-05-18 | 2005-05-18 | Luciferase-Assaysystem |
| PCT/NL2006/000246 WO2006121331A1 (en) | 2005-05-13 | 2006-05-12 | Use of ammonium and phosphate ions for improving the detection of luciferase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE486136T1 true ATE486136T1 (de) | 2010-11-15 |
Family
ID=34982311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06733050T ATE486136T1 (de) | 2005-05-13 | 2006-05-12 | Verwendung von ammonium- und phosphationen zur verbesserung des nachweises von luciferase |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US7425422B2 (de) |
| EP (2) | EP1724359A1 (de) |
| JP (1) | JP5254008B2 (de) |
| KR (1) | KR101396691B1 (de) |
| CN (1) | CN101218354B (de) |
| AT (1) | ATE486136T1 (de) |
| AU (1) | AU2006244734B2 (de) |
| CA (1) | CA2608337C (de) |
| DE (1) | DE602006017827D1 (de) |
| WO (1) | WO2006121331A1 (de) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2087132B1 (de) * | 2006-10-24 | 2017-01-25 | Gene Stream Pty Ltd. | Luciferase-signal verstärkende zusammensetzungen |
| AU2007335194B2 (en) | 2006-12-21 | 2014-01-23 | Gene Stream Pty Ltd | Bioluminescent assays utilising secreted luciferases |
| US9399657B2 (en) * | 2007-10-16 | 2016-07-26 | Tianxin Wang | Chemiluminescent methods and reagents for analyte detection |
| EP2419445A2 (de) | 2009-04-16 | 2012-02-22 | Forsyth Dental Infirmary for Children | Neue verfahren zur herstellung eines antikörpers und zusammensetzungen davon |
| JP6075723B2 (ja) * | 2012-08-21 | 2017-02-08 | 栄研化学株式会社 | Ｎ−（２−アセトアミド）イミノ二酢酸を用いる発光増強方法 |
| CN102879343B (zh) * | 2012-09-21 | 2014-10-29 | 重庆医科大学 | 用多波长吸收单通道同步测量多种酶活性的方法 |
| RU2596398C1 (ru) * | 2015-02-25 | 2016-09-10 | Закрытое акционерное общество "ЕВРОГЕН" | Метод и реактивы для детекции активности люциферазы |
| CN104975068B (zh) * | 2015-07-20 | 2016-09-07 | 北京中科紫鑫科技有限责任公司 | 一种检测样品中的荧光素酶活性的方法 |
| CN104946728B (zh) * | 2015-07-20 | 2016-08-17 | 北京中科紫鑫科技有限责任公司 | 一种萤火虫荧光素酶活性的测定方法 |
| CN105277537B (zh) * | 2015-10-12 | 2019-04-16 | 江苏三联生物工程有限公司 | 一种检测酶活性及化学发光反应底物性能的方法 |
| CN108330161A (zh) * | 2018-01-15 | 2018-07-27 | 厦门海荭兴仪器股份有限公司 | 一种热稳定性显著的atp荧光检测试剂 |
| CN111089859A (zh) * | 2018-10-23 | 2020-05-01 | 北京中医药大学 | 新型三磷酸腺苷生物发光测定方法及其用途 |
| CN110274896B (zh) * | 2019-03-15 | 2021-11-30 | 李文杰 | ATP生物荧光lgCB-lgIB标曲法评价液体消毒剂真菌杀灭效果的方法 |
| JP2022535789A (ja) | 2019-05-30 | 2022-08-10 | ４９０ バイオテック， インコーポレイテッド | 細胞におけるｌｕｘ発現と使用の方法 |
| JP7763771B2 (ja) * | 2020-05-12 | 2025-11-04 | ロンザ ヒューストン インコーポレイテッド | アデノ随伴ウイルスを検出するための方法およびキット |
| CN112195218B (zh) * | 2020-08-07 | 2024-10-29 | 苏州优逸兰迪生物科技有限公司 | 基于荧光素酶报告基因的生物活性检测试剂 |
| CN114350626A (zh) * | 2021-12-21 | 2022-04-15 | 合肥巅峰生物科技有限公司 | 一种荧光素酶冻干粉稀释反应液 |
| CN114736949B (zh) * | 2022-03-22 | 2024-03-01 | 药科元(上海)生物技术有限公司 | 一种萤火虫荧光素酶报告基因检测试剂盒 |
| CN115820792B (zh) * | 2022-12-19 | 2024-11-08 | 江苏凯基生物技术股份有限公司 | 一种检测细胞培养过程中支原体污染的试剂组合物、试剂盒及方法 |
| CN120028298B (zh) * | 2024-12-18 | 2025-11-18 | 中船海神医疗科技有限公司 | 一种高灵敏度的atp荧光检测方法及系统 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4950588A (en) * | 1985-07-10 | 1990-08-21 | Molecular Diagnostics, Inc. | Prolonged enhanced chemiluminescence |
| US5283179A (en) * | 1990-09-10 | 1994-02-01 | Promega Corporation | Luciferase assay method |
| NL1010224C2 (nl) * | 1998-09-30 | 2000-03-31 | Packard Biosciene B V | Werkwijze voor het detecteren van ATP. |
| AU2002348287A1 (en) * | 2001-11-16 | 2003-06-10 | Pierce Biotechnology, Inc. | Phosphine-containing formulations for chemiluminescent luciferase assays |
| JP2004089117A (ja) * | 2002-09-03 | 2004-03-25 | Toyo B-Net Co Ltd | ルシフェラーゼ検出試薬キット及び当該キットを使用したルシフェラーゼ検出方法 |
| US7556933B2 (en) * | 2004-10-01 | 2009-07-07 | Luminultra Technologies Ltd. | Reagent system and process for adenosine triphosphate monitoring |
-
2005
- 2005-05-18 EP EP05076165A patent/EP1724359A1/de not_active Withdrawn
-
2006
- 2006-05-12 US US11/433,791 patent/US7425422B2/en active Active
- 2006-05-12 WO PCT/NL2006/000246 patent/WO2006121331A1/en not_active Ceased
- 2006-05-12 CA CA2608337A patent/CA2608337C/en active Active
- 2006-05-12 EP EP06733050A patent/EP1885874B1/de active Active
- 2006-05-12 AU AU2006244734A patent/AU2006244734B2/en active Active
- 2006-05-12 DE DE602006017827T patent/DE602006017827D1/de active Active
- 2006-05-12 CN CN2006800251785A patent/CN101218354B/zh active Active
- 2006-05-12 KR KR1020077029083A patent/KR101396691B1/ko active Active
- 2006-05-12 AT AT06733050T patent/ATE486136T1/de not_active IP Right Cessation
- 2006-05-12 JP JP2008511069A patent/JP5254008B2/ja active Active
-
2008
- 2008-04-08 US US12/099,616 patent/US7951551B2/en active Active
- 2008-09-12 US US12/209,642 patent/US20090047693A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20070054342A1 (en) | 2007-03-08 |
| EP1885874B1 (de) | 2010-10-27 |
| US7951551B2 (en) | 2011-05-31 |
| CA2608337C (en) | 2015-04-07 |
| CN101218354B (zh) | 2011-09-07 |
| CA2608337A1 (en) | 2006-11-16 |
| EP1885874A1 (de) | 2008-02-13 |
| KR20080025061A (ko) | 2008-03-19 |
| US20090053744A1 (en) | 2009-02-26 |
| KR101396691B1 (ko) | 2014-05-21 |
| US20090047693A1 (en) | 2009-02-19 |
| JP2008545378A (ja) | 2008-12-18 |
| EP1724359A1 (de) | 2006-11-22 |
| US7425422B2 (en) | 2008-09-16 |
| AU2006244734B2 (en) | 2011-07-14 |
| WO2006121331A1 (en) | 2006-11-16 |
| CN101218354A (zh) | 2008-07-09 |
| AU2006244734A1 (en) | 2006-11-16 |
| DE602006017827D1 (de) | 2010-12-09 |
| JP5254008B2 (ja) | 2013-08-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |