ATE442445T1 - Rhamnose-induzierbares expressionssystem - Google Patents

Rhamnose-induzierbares expressionssystem

Info

Publication number
ATE442445T1
ATE442445T1 AT07013756T AT07013756T ATE442445T1 AT E442445 T1 ATE442445 T1 AT E442445T1 AT 07013756 T AT07013756 T AT 07013756T AT 07013756 T AT07013756 T AT 07013756T AT E442445 T1 ATE442445 T1 AT E442445T1
Authority
AT
Austria
Prior art keywords
rhamnose
inducable
expression system
interest
host cell
Prior art date
Application number
AT07013756T
Other languages
German (de)
English (en)
Inventor
Michael R Slater
Ethan Edward Strauss
Keith V Wood
James Robert Hartnett
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/678,961 external-priority patent/US8293503B2/en
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE442445T1 publication Critical patent/ATE442445T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
    • G16B50/30—Data warehousing; Computing architectures
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Databases & Information Systems (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioethics (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Optical Communication System (AREA)
AT07013756T 2003-10-03 2004-09-29 Rhamnose-induzierbares expressionssystem ATE442445T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/678,961 US8293503B2 (en) 2003-10-03 2003-10-03 Vectors for directional cloning
US10/702,228 US20050074785A1 (en) 2003-10-03 2003-11-05 Vectors for directional cloning

Publications (1)

Publication Number Publication Date
ATE442445T1 true ATE442445T1 (de) 2009-09-15

Family

ID=34971637

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07013756T ATE442445T1 (de) 2003-10-03 2004-09-29 Rhamnose-induzierbares expressionssystem

Country Status (6)

Country Link
US (1) US20070212762A1 (OSRAM)
EP (2) EP1847611B1 (OSRAM)
JP (1) JP5089984B2 (OSRAM)
AT (1) ATE442445T1 (OSRAM)
CA (1) CA2541177A1 (OSRAM)
WO (1) WO2005087932A2 (OSRAM)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
WO2006122354A1 (en) * 2005-05-17 2006-11-23 Ozgene Pty Ltd Sequential cloning system
AU2008212907A1 (en) * 2007-02-05 2008-08-14 Philipps-Universitat Marburg Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases
JPWO2009025209A1 (ja) 2007-08-21 2010-11-25 国立大学法人北海道大学 組換えｄｎａの調製方法
WO2009079212A2 (en) 2007-12-03 2009-06-25 Carnegie Mellon University Linked peptide fluorogenic biosensors
CA2716905A1 (en) 2008-02-29 2009-09-03 Xbrane Bioscience Aktiebolag Expression system for proteins
US20140363853A1 (en) * 2012-01-13 2014-12-11 Momenta Pharmaceuticals. Inc, Nucleotide cloning methods
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
WO2014151282A1 (en) 2013-03-15 2014-09-25 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
WO2014142955A1 (en) * 2013-03-15 2014-09-18 Carnegie Mellon University Linked peptide fluorogenic biosensors
CN104450919A (zh) * 2014-12-12 2015-03-25 南华大学 通过插入部分摇摆寡核苷酸检测基因突变耐受能力的方法
JPWO2021241593A1 (OSRAM) * 2020-05-26 2021-12-02

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4403036A (en) * 1980-12-02 1983-09-06 University Of Iowa Research Foundation Genetic reagents for generating plasmids containing multiple copies of DNA segments
US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
US4784949A (en) * 1984-04-19 1988-11-15 Cetus Corporation Universal dominant selectable marker cassette
US4952496A (en) * 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5693489A (en) * 1984-03-30 1997-12-02 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
EP0178863A1 (en) * 1984-10-15 1986-04-23 Schering Corporation Novel expression systems utilizing bacteriophage T7 promoters and gene sequences
US5200333A (en) * 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
EP0248678B1 (en) * 1986-06-06 1994-11-02 New England Biolabs, Inc. Method for cloning restriction modification system
US5506126A (en) * 1988-02-25 1996-04-09 The General Hospital Corporation Rapid immunoselection cloning method
EP0773294A3 (en) * 1989-07-28 1997-09-17 Us Health Powerful system for targeted genetic cloning
US5122457A (en) * 1989-10-19 1992-06-16 Schering Corporation Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase
EP0437100B1 (en) * 1990-01-11 1995-12-13 New England Biolabs, Inc. SfiI restriction endonuclease and methylase genes and Method for their cloning.
US5179015A (en) * 1990-07-23 1993-01-12 New England Biolabs, Inc. Heterospecific modification as a means to clone restriction genes
CA2095624C (en) * 1990-11-09 2000-06-13 Rafael D. Camerini-Otero Method of targeting dna
US5196330A (en) * 1991-06-03 1993-03-23 New England Biolabs, Inc. Type ii restriction endonuclease, pme i, obtainable from pseudomonas mendocina and a process for producing the same
US5342782A (en) * 1992-02-24 1994-08-30 Washington University Tightly regulated expression system for eucaryotic cells
BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US6521425B2 (en) * 1997-03-05 2003-02-18 New England Biolabs, Inc. Screening and use of reagents which block or activate intein splicing utilizing natural or homologous exteins
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
US5391487A (en) * 1993-08-13 1995-02-21 Promega Corporation Restriction endonuclease SgfI from Streptomyces griseoruber
US5498535A (en) * 1994-05-24 1996-03-12 New England Biolabs, Inc. Method for direct cloning of nuclease genes in E. coli
DE19514310A1 (de) * 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
EP0842283A1 (en) * 1995-07-21 1998-05-20 THE UNITED STATES GOVERNMENT as represented by THE DEPARTMENT OF HEALTH AND HUMAN SERVICES RecA-ASSISTED CLONING OF DNA
US5830690A (en) * 1995-12-29 1998-11-03 Council Of Scientific & Indus. Res. & Dept. Of Biotech. Process for producing polypeptides
US5663067A (en) * 1996-07-11 1997-09-02 New England Biolabs, Inc. Method for cloning and producing the SapI restriction endonuclease in E. coli
DE19653498C1 (de) * 1996-12-20 1998-07-23 Wolf M Prof Dr Bertling Klonierungsvektor-System mit Fusionssequenz
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6248569B1 (en) * 1997-11-10 2001-06-19 Brookhaven Science Associates Method for introducing unidirectional nested deletions
US5945288A (en) * 1997-11-24 1999-08-31 New England Biolabs, Inc. Method for cloning and producing the PmeI restriction endonuclease
US6579682B1 (en) * 1998-03-10 2003-06-17 The Regents Of University Of California Methods and tools for identifying compounds which modulate atherosclerosis by impacting LDL-proteoglycan binding
WO2000012687A1 (en) * 1998-08-28 2000-03-09 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6096523A (en) * 1998-11-04 2000-08-01 University Of Georgia Research Foundation Transformation vector system
US6184000B1 (en) * 1999-07-23 2001-02-06 The United States Of America As Represented By The Secretary Of Agriculture System for the sequential, directional cloning of multiple DNA sequences
US6479280B1 (en) * 1999-09-24 2002-11-12 Vlaams Interuniversitair Institutuut Voor Biotechnologie Vzw Recombinant phages capable of entering host cells via specific interaction with an artificial receptor
WO2001055369A1 (en) * 2000-01-28 2001-08-02 The Scripps Research Institute Synthetic internal ribosome entry sites and methods of identifying same
US6908751B2 (en) * 2000-04-26 2005-06-21 Zymogenetics, Inc. Methods for enhancing the expression of a protein of interest by recombinant host cells
US20020086310A1 (en) * 2000-09-01 2002-07-04 Frank Fan Identification of targets of antimicrobial compounds
US6800453B2 (en) * 2001-01-23 2004-10-05 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
FR2824844B1 (fr) * 2001-05-18 2003-09-19 Genoway Procede de clonage par double selection et vecteurs pour ce procede
US7094579B2 (en) * 2002-02-13 2006-08-22 Xoma Technology Ltd. Eukaryotic signal sequences for prokaryotic expression
EP1576140A4 (en) * 2002-09-26 2007-08-08 Kosan Biosciences Inc ARTIFICIAL GENES
DE50307835D1 (de) * 2002-12-02 2007-09-13 Basf Ag L-rhamnose-induzierbare expressionssysteme
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning

Also Published As

Publication number Publication date
EP1685247A2 (en) 2006-08-02
US20070212762A1 (en) 2007-09-13
EP1847611B1 (en) 2009-09-09
JP5089984B2 (ja) 2012-12-05
EP1847611A3 (en) 2007-10-31
CA2541177A1 (en) 2005-09-22
JP2007508012A (ja) 2007-04-05
EP1685247B1 (en) 2009-11-11
EP1847611A2 (en) 2007-10-24
WO2005087932A3 (en) 2006-03-02
WO2005087932A2 (en) 2005-09-22

Similar Documents

Publication Publication Date Title
ATE442445T1 (de) Rhamnose-induzierbares expressionssystem
DK2048236T3 (da) Genekspressionsteknik
DK1042461T3 (da) Ekspressionskloning i filiforme svampe
MY169472A (en) Refolding of recombinant proteins
DK1944318T3 (da) Multifunktionelle cytokiner
SG134283A1 (en) Biomarkers for liver fibrotic injury
ATE348881T1 (de) Thermostabiles taq polymerase fragment
ATE454469T1 (de) Für den nachweis von nukleinsäuren kompetente biologische rohderivate
DK1697523T3 (da) Ekspressionsvektor til antibiotikafri ekspression
DE602004029598D1 (de) Minimale dna sequenz, die als chromatin-isolator wirkt, und deren verwendung für die protein-expression
DE602004026912D1 (de) Formulierungen und verfahren zum denaturieren von proteinen
WO2005047881A3 (en) Repetitive reversed-field affinity electrophoresis and uses therefor
DE60041243D1 (de) Gebrauchsverfaheren eines neuen lysyloxidase-verwandten proteins
NO20070757L (no) Ny sekvens for forbedring av ekspresjon av nukleinsyre(r)
ATE469984T1 (de) Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
DE60333486D1 (de) Fluoreszenzproteine aus copepoda-spezies und verfahren zur verwendung davon
ATE488591T1 (de) Neuer l-lysin-induzierbarer promotor
ATE539163T1 (de) Auf der verwendung von mva beruhende impfstoffe
DE60041042D1 (de) Chlamydia antigene und deren korrespondierende dna fragmente und verwendungen davon
DK1630232T3 (da) Optimerede nukleotidsekvenser kodende for sgp130
DE60136022D1 (de) Mittel und Verfahren zur Expression von homologen und heterologen Proteinen in Rhodococcus
ATE546534T1 (de) Expression system für rekombinante proteine
DK2186898T3 (da) Blomstrings-induktion
DE60225046D1 (de) Produktion eukaryontischer proteine und nukleinsäuremoleküle in c. elegans
ATE547427T1 (de) Verfahren zur rekombinanten herstellung von ribonukleoproteinen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties