ATE429494T1 - Verfahren zur isolierung von mrna aus mit formalin fixiertem und in paraffin eingebettetem gewebe - Google Patents

Verfahren zur isolierung von mrna aus mit formalin fixiertem und in paraffin eingebettetem gewebe

Info

Publication number
ATE429494T1
ATE429494T1 AT06807415T AT06807415T ATE429494T1 AT E429494 T1 ATE429494 T1 AT E429494T1 AT 06807415 T AT06807415 T AT 06807415T AT 06807415 T AT06807415 T AT 06807415T AT E429494 T1 ATE429494 T1 AT E429494T1
Authority
AT
Austria
Prior art keywords
formalin
paraffin
sample
solution
fixed
Prior art date
Application number
AT06807415T
Other languages
English (en)
Inventor
Costa Rafael Rosell
Roca Miguel Taran
Original Assignee
Pangaea Biotech Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pangaea Biotech Sa filed Critical Pangaea Biotech Sa
Application granted granted Critical
Publication of ATE429494T1 publication Critical patent/ATE429494T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
AT06807415T 2005-10-20 2006-10-19 Verfahren zur isolierung von mrna aus mit formalin fixiertem und in paraffin eingebettetem gewebe ATE429494T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05077417A EP1777291A1 (de) 2005-10-20 2005-10-20 Verfahren zur Isolierung von mRNS aus Formalin-fixierten, Paraffin-eingebetteten Gewebeproben
PCT/EP2006/067591 WO2007045681A1 (en) 2005-10-20 2006-10-19 Method for the isolation of mrna from formalin fixed, paraffin-embedded tissue

Publications (1)

Publication Number Publication Date
ATE429494T1 true ATE429494T1 (de) 2009-05-15

Family

ID=36129752

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06807415T ATE429494T1 (de) 2005-10-20 2006-10-19 Verfahren zur isolierung von mrna aus mit formalin fixiertem und in paraffin eingebettetem gewebe

Country Status (12)

Country Link
US (1) US8188265B2 (de)
EP (2) EP1777291A1 (de)
AT (1) ATE429494T1 (de)
AU (1) AU2006303221B2 (de)
CA (1) CA2626533C (de)
CY (1) CY1115586T1 (de)
DE (1) DE602006006468D1 (de)
DK (1) DK1945764T3 (de)
ES (1) ES2326341T3 (de)
HK (1) HK1119738A1 (de)
PT (1) PT1945764E (de)
WO (1) WO2007045681A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008043332A (ja) * 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量
JP2013531987A (ja) * 2010-06-14 2013-08-15 キアゲン ゲーエムベーハー 固定生物学的試料から生体分子を抽出するためのターゲット細胞または組織を決定するための方法
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
US9957550B2 (en) 2014-09-08 2018-05-01 BioSpyder Technologies, Inc. Attenuators
US9938566B2 (en) 2014-09-08 2018-04-10 BioSpyder Technologies, Inc. Profiling expression at transcriptome scale
AU2015319806A1 (en) * 2014-09-26 2017-04-20 The Regents Of The University Of California Methods and systems for detection of a genetic mutation
CN107271239A (zh) * 2017-07-06 2017-10-20 浙江大学 一种溶解石蜡组织切片的方法及应用
EP3668978A4 (de) 2017-08-18 2021-04-21 Fluidigm Corporation Verfahren und kits zur extraktion von nukleinsäuren aus paraffineingebetteten proben
US20230140574A1 (en) * 2020-03-31 2023-05-04 Qiagen Gmbh Nucleic acid purification from fixed biological samples

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6469159B1 (en) * 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

Also Published As

Publication number Publication date
EP1945764A1 (de) 2008-07-23
ES2326341T3 (es) 2009-10-07
EP1945764B1 (de) 2009-04-22
PT1945764E (pt) 2009-07-27
US20090264641A1 (en) 2009-10-22
DK1945764T3 (da) 2009-08-17
EP1777291A1 (de) 2007-04-25
CA2626533A1 (en) 2007-04-26
AU2006303221B2 (en) 2011-09-08
AU2006303221A1 (en) 2007-04-26
CY1115586T1 (el) 2017-01-04
US8188265B2 (en) 2012-05-29
WO2007045681A1 (en) 2007-04-26
CA2626533C (en) 2013-03-19
DE602006006468D1 (de) 2009-06-04
HK1119738A1 (en) 2009-03-13

Similar Documents

Publication Publication Date Title
ATE429494T1 (de) Verfahren zur isolierung von mrna aus mit formalin fixiertem und in paraffin eingebettetem gewebe
Okello et al. Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues
ATE489482T1 (de) Verfahren zur herstellung von hybridsubstraten mit dna und antikörpern sowie verwendungen davon
WO2009076485A3 (en) Sequencing of nucleic acids
Li et al. Brassinosteroids-induced systemic stress tolerance was associated with increased transcripts of several defence-related genes in the phloem in Cucumis sativus
EP3650559B1 (de) Direkte rna-nanoporensequenzierung mit hilfe eines stammschleifenpolynukleotids
EP1994142A4 (de) Verfahren und zusammensetzungen zur schnellen isolierung kleiner rna-moleküle
Arandia‐Gorostidi et al. Testing the metabolic theory of ecology with marine bacteria: different temperature sensitivity of major phylogenetic groups during the spring phytoplankton bloom
ATE555202T1 (de) Verfahren zur lieferung von rna-interferenz und verwendungen damit
JP2003525033A5 (de)
Liu et al. Diatoms shape the biogeography of heterotrophic prokaryotes in early spring in the Southern Ocean
ATE515488T1 (de) Click-chemie zur herstellung von reportermolekülen
ATE541054T1 (de) Bio-barcode erkennung für zielmoleküle
ATE515573T1 (de) Verfahren zum nachweis der grösse von nukleinsäuren
ATE492813T1 (de) Verfahren zur isolierung von analyten aus einer probe
WO2009002937A3 (en) Methods for isolating long fragment rna from fixed samples
ATE430812T1 (de) Materialien und verfahren zum einfangen von krankheitserregern und zur abtrennung von aurintricarbonsäure aus einer probe
DK1272843T3 (da) Fremgangsmåde til identifikation af regulatorer af protein-AGE-dannelse
ATE533838T1 (de) Verfahren zur verbesserten einführung von dna in bakterienzellen
Libus et al. Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization
ATE541945T1 (de) Nachweis und quantifizierung fäkaler verunreinigungen in umweltproben
DE602007009498D1 (de) Neue fluoreszenzproteine und verfahren zur verwendung davon
Sindelka et al. Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan
WO2006012302A3 (en) Methods for rna fluorescence in situ hybridization
CN109161586B (zh) 一种对rna分子进行绝对定量的高通量测序方法

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1945764

Country of ref document: EP