ATE429494T1 - Verfahren zur isolierung von mrna aus mit formalin fixiertem und in paraffin eingebettetem gewebe - Google Patents
Verfahren zur isolierung von mrna aus mit formalin fixiertem und in paraffin eingebettetem gewebeInfo
- Publication number
- ATE429494T1 ATE429494T1 AT06807415T AT06807415T ATE429494T1 AT E429494 T1 ATE429494 T1 AT E429494T1 AT 06807415 T AT06807415 T AT 06807415T AT 06807415 T AT06807415 T AT 06807415T AT E429494 T1 ATE429494 T1 AT E429494T1
- Authority
- AT
- Austria
- Prior art keywords
- formalin
- paraffin
- sample
- solution
- fixed
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05077417A EP1777291A1 (de) | 2005-10-20 | 2005-10-20 | Verfahren zur Isolierung von mRNS aus Formalin-fixierten, Paraffin-eingebetteten Gewebeproben |
| PCT/EP2006/067591 WO2007045681A1 (en) | 2005-10-20 | 2006-10-19 | Method for the isolation of mrna from formalin fixed, paraffin-embedded tissue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE429494T1 true ATE429494T1 (de) | 2009-05-15 |
Family
ID=36129752
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06807415T ATE429494T1 (de) | 2005-10-20 | 2006-10-19 | Verfahren zur isolierung von mrna aus mit formalin fixiertem und in paraffin eingebettetem gewebe |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US8188265B2 (de) |
| EP (2) | EP1777291A1 (de) |
| AT (1) | ATE429494T1 (de) |
| AU (1) | AU2006303221B2 (de) |
| CA (1) | CA2626533C (de) |
| CY (1) | CY1115586T1 (de) |
| DE (1) | DE602006006468D1 (de) |
| DK (1) | DK1945764T3 (de) |
| ES (1) | ES2326341T3 (de) |
| HK (1) | HK1119738A1 (de) |
| PT (1) | PT1945764E (de) |
| WO (1) | WO2007045681A1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008043332A (ja) * | 2006-08-17 | 2008-02-28 | Panomics Inc | 組織スライドからの核酸定量 |
| WO2011157678A1 (en) * | 2010-06-14 | 2011-12-22 | Qiagen Gmbh | Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples |
| TWI425977B (zh) | 2010-11-24 | 2014-02-11 | Ind Tech Res Inst | 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組 |
| US9938566B2 (en) | 2014-09-08 | 2018-04-10 | BioSpyder Technologies, Inc. | Profiling expression at transcriptome scale |
| US9957550B2 (en) | 2014-09-08 | 2018-05-01 | BioSpyder Technologies, Inc. | Attenuators |
| JP2017529855A (ja) * | 2014-09-26 | 2017-10-12 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 遺伝子突然変異の検出のための方法およびシステム |
| CN107271239A (zh) * | 2017-07-06 | 2017-10-20 | 浙江大学 | 一种溶解石蜡组织切片的方法及应用 |
| SG11202001088PA (en) | 2017-08-18 | 2020-03-30 | Fluidigm Corp | Methods and kits for extracting nucleic acids from paraffin embedded samples |
| CN115335519A (zh) * | 2020-03-31 | 2022-11-11 | 凯杰有限公司 | 从固定的生物样品纯化核酸 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6469159B1 (en) * | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US6248535B1 (en) * | 1999-12-20 | 2001-06-19 | University Of Southern California | Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens |
-
2005
- 2005-10-20 EP EP05077417A patent/EP1777291A1/de not_active Withdrawn
-
2006
- 2006-10-19 DE DE602006006468T patent/DE602006006468D1/de active Active
- 2006-10-19 EP EP06807415A patent/EP1945764B1/de active Active
- 2006-10-19 DK DK06807415T patent/DK1945764T3/da active
- 2006-10-19 CA CA2626533A patent/CA2626533C/en active Active
- 2006-10-19 US US12/090,767 patent/US8188265B2/en active Active
- 2006-10-19 WO PCT/EP2006/067591 patent/WO2007045681A1/en active Application Filing
- 2006-10-19 ES ES06807415T patent/ES2326341T3/es active Active
- 2006-10-19 AU AU2006303221A patent/AU2006303221B2/en not_active Ceased
- 2006-10-19 AT AT06807415T patent/ATE429494T1/de active
- 2006-10-19 PT PT06807415T patent/PT1945764E/pt unknown
-
2008
- 2008-10-21 HK HK08111595.8A patent/HK1119738A1/xx not_active IP Right Cessation
-
2009
- 2009-07-16 CY CY20091100760T patent/CY1115586T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2326341T3 (es) | 2009-10-07 |
| PT1945764E (pt) | 2009-07-27 |
| DK1945764T3 (da) | 2009-08-17 |
| EP1777291A1 (de) | 2007-04-25 |
| US20090264641A1 (en) | 2009-10-22 |
| AU2006303221B2 (en) | 2011-09-08 |
| HK1119738A1 (en) | 2009-03-13 |
| EP1945764B1 (de) | 2009-04-22 |
| CY1115586T1 (el) | 2017-01-04 |
| DE602006006468D1 (de) | 2009-06-04 |
| WO2007045681A1 (en) | 2007-04-26 |
| CA2626533A1 (en) | 2007-04-26 |
| CA2626533C (en) | 2013-03-19 |
| EP1945764A1 (de) | 2008-07-23 |
| US8188265B2 (en) | 2012-05-29 |
| AU2006303221A1 (en) | 2007-04-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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