ATE419329T1 - Verfahren zur kontrolle von polypeptidproduktion und -aufreinigung mit oberflächen verstärkter laserdesorption/ionisations massenspektrometrie - Google Patents
Verfahren zur kontrolle von polypeptidproduktion und -aufreinigung mit oberflächen verstärkter laserdesorption/ionisations massenspektrometrieInfo
- Publication number
- ATE419329T1 ATE419329T1 AT02792236T AT02792236T ATE419329T1 AT E419329 T1 ATE419329 T1 AT E419329T1 AT 02792236 T AT02792236 T AT 02792236T AT 02792236 T AT02792236 T AT 02792236T AT E419329 T1 ATE419329 T1 AT E419329T1
- Authority
- AT
- Austria
- Prior art keywords
- laser desorption
- purification
- ionization mass
- enhanced laser
- mass spectrometry
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0409—Sample holders or containers
- H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/84—Preparation of the fraction to be distributed
- G01N2030/8411—Intermediate storage of effluent, including condensation on surface
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33560901P | 2001-11-14 | 2001-11-14 | |
US10/124,626 US20030091976A1 (en) | 2001-11-14 | 2002-04-16 | Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE419329T1 true ATE419329T1 (de) | 2009-01-15 |
Family
ID=26822785
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02792236T ATE419329T1 (de) | 2001-11-14 | 2002-11-08 | Verfahren zur kontrolle von polypeptidproduktion und -aufreinigung mit oberflächen verstärkter laserdesorption/ionisations massenspektrometrie |
Country Status (8)
Country | Link |
---|---|
US (2) | US20030091976A1 (de) |
EP (1) | EP1448760B1 (de) |
JP (1) | JP2005509173A (de) |
AT (1) | ATE419329T1 (de) |
AU (1) | AU2002357699A1 (de) |
CA (1) | CA2465214A1 (de) |
DE (1) | DE60230660D1 (de) |
WO (1) | WO2003042233A2 (de) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003256806A1 (en) | 2002-07-24 | 2004-02-09 | Ciphergen Biosystems, Inc. | Protein interaction difference mapping |
WO2004061410A2 (en) * | 2002-12-18 | 2004-07-22 | Ciphergen Biosystems, Inc. | Serum biomarkers in lung cancer |
EP1616180A4 (de) * | 2003-04-22 | 2006-07-19 | Ciphergen Biosystems Inc | Analyseverfahren für wirtszellproteine |
US7529629B2 (en) * | 2003-04-28 | 2009-05-05 | Cerno Bioscience Llc | Computational methods and systems for multidimensional analysis |
US20050164398A1 (en) * | 2004-01-26 | 2005-07-28 | Alexander James N.Iv | Method for determining molecular weight of polymers |
US7906630B2 (en) * | 2004-04-27 | 2011-03-15 | PerkinElmer Heath Sciences, Inc. | Method for identifying peptides in a biological sample |
JP5008564B2 (ja) * | 2004-05-20 | 2012-08-22 | ウオーターズ・テクノロジーズ・コーポレイシヨン | 混合物中のタンパク質を同定する方法および装置 |
EP1781778A4 (de) * | 2004-06-16 | 2007-09-12 | Ciphergen Biosystems Inc | Multichemiefraktionierung |
JP4659831B2 (ja) * | 2005-07-29 | 2011-03-30 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | 化合物に対するタンパク質の結合力を解析する方法 |
JP2007108015A (ja) * | 2005-10-13 | 2007-04-26 | Biologica:Kk | 分析用の保持体及びその利用 |
KR101618144B1 (ko) * | 2007-12-31 | 2016-05-17 | 박스알타 인코퍼레이티드 | 실질적으로 동물 단백질-부재인 재조합 푸린 및 이의 제조 방법 |
EP2255188B1 (de) * | 2008-02-29 | 2016-08-24 | Imec | Biosensor zum nachweis von neurotransmittern |
JP5365116B2 (ja) * | 2008-09-19 | 2013-12-11 | 横河電機株式会社 | イオンクロマトグラフ測定装置およびイオンクロマトグラフ測定方法 |
US20140166875A1 (en) | 2010-09-02 | 2014-06-19 | Wayne State University | Systems and methods for high throughput solvent assisted ionization inlet for mass spectrometry |
US9552973B2 (en) * | 2010-09-02 | 2017-01-24 | University Of The Sciences In Philadelphia | System and method for ionization of molecules for mass spectrometry and ion mobility spectrometry |
WO2012058248A2 (en) | 2010-10-25 | 2012-05-03 | Wayne State University | Systems and methods extending the laserspray ionization mass spectrometry concept from atmospheric pressure to vacuum |
WO2012082933A1 (en) | 2010-12-15 | 2012-06-21 | Baxter International, Inc. | Eluate collection using conductivity gradient |
US8506797B2 (en) * | 2011-04-10 | 2013-08-13 | Therapeutic Proteins International, LLC | Downstream bioprocessing device |
EP2970865B1 (de) | 2013-03-15 | 2020-09-02 | Alder Biopharmaceuticals, Inc. | Antikörperreinigung und -reinheitsüberwachung |
US9891203B2 (en) * | 2013-06-05 | 2018-02-13 | Dh Technologies Development Pte. Ltd. | SWATH# data-independent acquisition technology for the detection of host cell protein contaminants in biotherapeutics protein products |
TW202204596A (zh) * | 2014-06-06 | 2022-02-01 | 美商健臻公司 | 灌注培養方法及其用途 |
EP3443348A1 (de) * | 2016-04-14 | 2019-02-20 | Lonza Ltd | Zusammensetzungen und verfahren zur detektion von wirtszellenproteinen |
CN109118004B (zh) * | 2018-08-16 | 2021-09-14 | 李宏伟 | 一种工程构筑选址适宜区预测方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2201077T3 (es) * | 1993-05-28 | 2004-03-16 | Baylor College Of Medicine | Metodo y espectrometro de masas para la desorcion e ionizacion de analitos. |
US20020151707A1 (en) * | 1995-06-07 | 2002-10-17 | Corixa Corp. | Immune mediators and related methods |
AU3374597A (en) * | 1996-06-07 | 1998-01-05 | Immunivest Corporation | Magnetic separation employing external and internal gradients |
ES2164297T3 (es) * | 1996-09-09 | 2002-02-16 | Rmf Dictagene Sa | Preparacion de (poli)peptidos purificados. |
NZ516848A (en) * | 1997-06-20 | 2004-03-26 | Ciphergen Biosystems Inc | Retentate chromatography apparatus with applications in biology and medicine |
US6207370B1 (en) * | 1997-09-02 | 2001-03-27 | Sequenom, Inc. | Diagnostics based on mass spectrometric detection of translated target polypeptides |
US6210910B1 (en) * | 1998-03-02 | 2001-04-03 | Trustees Of Tufts College | Optical fiber biosensor array comprising cell populations confined to microcavities |
JP3345401B2 (ja) * | 1998-08-25 | 2002-11-18 | ユニバーシティ オブ ワシントン | 複合した混合物中のタンパク質またはタンパク質機能の迅速定量分析 |
US6558946B1 (en) * | 2000-08-29 | 2003-05-06 | The United States Of America As Represented By The Secretary Of The Army | Automated sample processing for identification of microorganisms and proteins |
-
2002
- 2002-04-16 US US10/124,626 patent/US20030091976A1/en not_active Abandoned
- 2002-11-08 AT AT02792236T patent/ATE419329T1/de not_active IP Right Cessation
- 2002-11-08 DE DE60230660T patent/DE60230660D1/de not_active Expired - Fee Related
- 2002-11-08 WO PCT/US2002/035971 patent/WO2003042233A2/en active Application Filing
- 2002-11-08 JP JP2003544068A patent/JP2005509173A/ja active Pending
- 2002-11-08 EP EP02792236A patent/EP1448760B1/de not_active Expired - Lifetime
- 2002-11-08 AU AU2002357699A patent/AU2002357699A1/en not_active Abandoned
- 2002-11-08 CA CA002465214A patent/CA2465214A1/en not_active Abandoned
-
2006
- 2006-08-03 US US11/499,898 patent/US20070059776A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU2002357699A1 (en) | 2003-05-26 |
WO2003042233A3 (en) | 2004-01-08 |
CA2465214A1 (en) | 2003-05-22 |
US20030091976A1 (en) | 2003-05-15 |
US20070059776A1 (en) | 2007-03-15 |
WO2003042233A2 (en) | 2003-05-22 |
JP2005509173A (ja) | 2005-04-07 |
EP1448760B1 (de) | 2008-12-31 |
EP1448760A4 (de) | 2005-01-05 |
DE60230660D1 (de) | 2009-02-12 |
EP1448760A2 (de) | 2004-08-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |