ATE419329T1 - METHOD FOR CONTROLLING POLYPEPTIDE PRODUCTION AND PURIFICATION USING SURFACES ENHANCED LASER DESORPTION/IONIZATION MASS SPECTROMETRY - Google Patents

METHOD FOR CONTROLLING POLYPEPTIDE PRODUCTION AND PURIFICATION USING SURFACES ENHANCED LASER DESORPTION/IONIZATION MASS SPECTROMETRY

Info

Publication number
ATE419329T1
ATE419329T1 AT02792236T AT02792236T ATE419329T1 AT E419329 T1 ATE419329 T1 AT E419329T1 AT 02792236 T AT02792236 T AT 02792236T AT 02792236 T AT02792236 T AT 02792236T AT E419329 T1 ATE419329 T1 AT E419329T1
Authority
AT
Austria
Prior art keywords
laser desorption
purification
ionization mass
enhanced laser
mass spectrometry
Prior art date
Application number
AT02792236T
Other languages
German (de)
Inventor
Egisto Boschetti
Lisa Bradbury
Huw Davies
Lee Lomas
Thang Pham
Vanitha Thulasiraman
Tai-Tung Yip
Original Assignee
Biorad Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biorad Lab Inc filed Critical Biorad Lab Inc
Application granted granted Critical
Publication of ATE419329T1 publication Critical patent/ATE419329T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8411—Intermediate storage of effluent, including condensation on surface
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes

Abstract

The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.
AT02792236T 2001-11-14 2002-11-08 METHOD FOR CONTROLLING POLYPEPTIDE PRODUCTION AND PURIFICATION USING SURFACES ENHANCED LASER DESORPTION/IONIZATION MASS SPECTROMETRY ATE419329T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33560901P 2001-11-14 2001-11-14
US10/124,626 US20030091976A1 (en) 2001-11-14 2002-04-16 Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry

Publications (1)

Publication Number Publication Date
ATE419329T1 true ATE419329T1 (en) 2009-01-15

Family

ID=26822785

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02792236T ATE419329T1 (en) 2001-11-14 2002-11-08 METHOD FOR CONTROLLING POLYPEPTIDE PRODUCTION AND PURIFICATION USING SURFACES ENHANCED LASER DESORPTION/IONIZATION MASS SPECTROMETRY

Country Status (8)

Country Link
US (2) US20030091976A1 (en)
EP (1) EP1448760B1 (en)
JP (1) JP2005509173A (en)
AT (1) ATE419329T1 (en)
AU (1) AU2002357699A1 (en)
CA (1) CA2465214A1 (en)
DE (1) DE60230660D1 (en)
WO (1) WO2003042233A2 (en)

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WO2004009798A2 (en) 2002-07-24 2004-01-29 Ciphergen Biosystems, Inc. Protein interaction difference mapping
US8014952B2 (en) 2002-12-18 2011-09-06 Queen Elizabeth Hospital Serum biomarkers in lung cancer
US20060205010A1 (en) * 2003-04-22 2006-09-14 Catherine Allioux Methods of host cell protein analysis
US7529629B2 (en) * 2003-04-28 2009-05-05 Cerno Bioscience Llc Computational methods and systems for multidimensional analysis
US20050164398A1 (en) * 2004-01-26 2005-07-28 Alexander James N.Iv Method for determining molecular weight of polymers
US7906630B2 (en) * 2004-04-27 2011-03-15 PerkinElmer Heath Sciences, Inc. Method for identifying peptides in a biological sample
GB2430794B (en) * 2004-05-20 2009-10-21 Waters Investments Ltd Method and apparatus for identifying proteins in mixtures
WO2006007429A1 (en) * 2004-06-16 2006-01-19 Ciphergen Biosystems, Inc. Multichemistry fractionation
WO2007013699A1 (en) * 2005-07-29 2007-02-01 Eisai R & D Management Co., Ltd. Method for analysis of compound-binding ability of protein
JP2007108015A (en) * 2005-10-13 2007-04-26 Biologica:Kk Holder for analysis and utilization thereof
KR101618144B1 (en) * 2007-12-31 2016-05-17 박스알타 인코퍼레이티드 Substantially animal protein-free recombinant furin and methods for producing same
JP5286370B2 (en) * 2008-02-29 2013-09-11 アイメック Cellular enzyme-based biosensor
JP5365116B2 (en) * 2008-09-19 2013-12-11 横河電機株式会社 Ion chromatograph measuring apparatus and ion chromatograph measuring method
WO2012031082A2 (en) * 2010-09-02 2012-03-08 University Of The Sciences In Philadelphia System and method for ionization of molecules for mass spectrometry and ion mobility spectrometry
US20140166875A1 (en) 2010-09-02 2014-06-19 Wayne State University Systems and methods for high throughput solvent assisted ionization inlet for mass spectrometry
US8853621B2 (en) 2010-10-25 2014-10-07 Wayne State University Systems and methods extending the laserspray ionization mass spectrometry concept from atmospheric pressure to vacuum
CA2821711C (en) 2010-12-15 2017-10-10 Baxter International Inc. Eluate collection using conductivity gradient
US8506797B2 (en) * 2011-04-10 2013-08-13 Therapeutic Proteins International, LLC Downstream bioprocessing device
CA2905531C (en) 2013-03-15 2023-01-03 Alder Biopharmaceuticals, Inc. Antibody purification and purity monitoring
US9891203B2 (en) * 2013-06-05 2018-02-13 Dh Technologies Development Pte. Ltd. SWATH# data-independent acquisition technology for the detection of host cell protein contaminants in biotherapeutics protein products
TW202204596A (en) * 2014-06-06 2022-02-01 美商健臻公司 Perfusion culturing methods and uses thereof
AU2017251360A1 (en) * 2016-04-14 2018-11-01 Lonza Ltd Compositions and methods for the detection of host cell proteins
CN109118004B (en) * 2018-08-16 2021-09-14 李宏伟 Prediction method for suitable area for engineering construction site selection

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
DE69432791T2 (en) * 1993-05-28 2004-06-03 Baylor College Of Medicine, Houston METHOD AND MASS SPECTROMETER FOR DESORPTION AND IONIZATION OF ANALYZES
US20020151707A1 (en) * 1995-06-07 2002-10-17 Corixa Corp. Immune mediators and related methods
US5993665A (en) * 1996-06-07 1999-11-30 Immunivest Corporation Quantitative cell analysis methods employing magnetic separation
PT827966E (en) * 1996-09-09 2002-05-31 Rmf Dictagene Sa PREPARATION OF PURIFIED (POLY) PEPTIDES
NZ516848A (en) * 1997-06-20 2004-03-26 Ciphergen Biosystems Inc Retentate chromatography apparatus with applications in biology and medicine
US6207370B1 (en) * 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6210910B1 (en) * 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
DE69912444T3 (en) * 1998-08-25 2010-05-06 University Of Washington, Seattle FAST QUANTITATIVE ANALYSIS OF PROTEINS OR PROTEIN FUNCTIONS IN COMPLEX MIXTURES
US6558946B1 (en) * 2000-08-29 2003-05-06 The United States Of America As Represented By The Secretary Of The Army Automated sample processing for identification of microorganisms and proteins

Also Published As

Publication number Publication date
EP1448760A2 (en) 2004-08-25
EP1448760B1 (en) 2008-12-31
US20070059776A1 (en) 2007-03-15
AU2002357699A1 (en) 2003-05-26
CA2465214A1 (en) 2003-05-22
WO2003042233A3 (en) 2004-01-08
JP2005509173A (en) 2005-04-07
DE60230660D1 (en) 2009-02-12
WO2003042233A2 (en) 2003-05-22
US20030091976A1 (en) 2003-05-15
EP1448760A4 (en) 2005-01-05

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