DE60230660D1 - Verfahren zur kontrolle von polypeptidproduktion udesorption/ionisations massenspektrometrie - Google Patents

Verfahren zur kontrolle von polypeptidproduktion udesorption/ionisations massenspektrometrie

Info

Publication number
DE60230660D1
DE60230660D1 DE60230660T DE60230660T DE60230660D1 DE 60230660 D1 DE60230660 D1 DE 60230660D1 DE 60230660 T DE60230660 T DE 60230660T DE 60230660 T DE60230660 T DE 60230660T DE 60230660 D1 DE60230660 D1 DE 60230660D1
Authority
DE
Germany
Prior art keywords
ionization mass
udesorption
samples taken
mass spectrometry
polypeptide production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60230660T
Other languages
English (en)
Inventor
Egisto Boschetti
Lisa Bradbury
Huw Davies
Lee Lomas
Thang T Pham
Vanitha Thulasiraman
Tai-Tung Yip
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Application granted granted Critical
Publication of DE60230660D1 publication Critical patent/DE60230660D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8411—Intermediate storage of effluent, including condensation on surface
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60230660T 2001-11-14 2002-11-08 Verfahren zur kontrolle von polypeptidproduktion udesorption/ionisations massenspektrometrie Expired - Fee Related DE60230660D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33560901P 2001-11-14 2001-11-14
US10/124,626 US20030091976A1 (en) 2001-11-14 2002-04-16 Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry
PCT/US2002/035971 WO2003042233A2 (en) 2001-11-14 2002-11-08 Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry

Publications (1)

Publication Number Publication Date
DE60230660D1 true DE60230660D1 (de) 2009-02-12

Family

ID=26822785

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60230660T Expired - Fee Related DE60230660D1 (de) 2001-11-14 2002-11-08 Verfahren zur kontrolle von polypeptidproduktion udesorption/ionisations massenspektrometrie

Country Status (8)

Country Link
US (2) US20030091976A1 (de)
EP (1) EP1448760B1 (de)
JP (1) JP2005509173A (de)
AT (1) ATE419329T1 (de)
AU (1) AU2002357699A1 (de)
CA (1) CA2465214A1 (de)
DE (1) DE60230660D1 (de)
WO (1) WO2003042233A2 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60326864D1 (de) * 2002-07-24 2009-05-07 Bio Rad Laboratories Proteininteraktionsdifferenzabbildung
EP1573044A4 (de) 2002-12-18 2006-07-05 Ciphergen Biosystems Inc Serum-biomarker bei lungenkrebs
EP1616180A4 (de) * 2003-04-22 2006-07-19 Ciphergen Biosystems Inc Analyseverfahren für wirtszellproteine
US7529629B2 (en) * 2003-04-28 2009-05-05 Cerno Bioscience Llc Computational methods and systems for multidimensional analysis
US20050164398A1 (en) * 2004-01-26 2005-07-28 Alexander James N.Iv Method for determining molecular weight of polymers
US7906630B2 (en) * 2004-04-27 2011-03-15 PerkinElmer Heath Sciences, Inc. Method for identifying peptides in a biological sample
DE112005001166B4 (de) * 2004-05-20 2014-10-09 Waters Technologies Corp. (N.D.Ges.D. Staates Delaware) Verfahren und Vorrichtung zum Identifizieren von Proteinen in Gemischen
JP2008503725A (ja) * 2004-06-16 2008-02-07 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド マルチケミストリ分画
US8889423B2 (en) * 2005-07-29 2014-11-18 Eisai R & D Management Co., Ltd. Method for analysis of compound-binding ability of protein
JP2007108015A (ja) * 2005-10-13 2007-04-26 Biologica:Kk 分析用の保持体及びその利用
ES2735173T3 (es) 2007-12-31 2019-12-16 Baxalta GmbH Furina recombinante sustancialmente libre de proteína animal y métodos para producir la misma
JP5286370B2 (ja) * 2008-02-29 2013-09-11 アイメック 細胞酵素ベースのバイオセンサ
JP5365116B2 (ja) * 2008-09-19 2013-12-11 横河電機株式会社 イオンクロマトグラフ測定装置およびイオンクロマトグラフ測定方法
US9552973B2 (en) * 2010-09-02 2017-01-24 University Of The Sciences In Philadelphia System and method for ionization of molecules for mass spectrometry and ion mobility spectrometry
US20140166875A1 (en) 2010-09-02 2014-06-19 Wayne State University Systems and methods for high throughput solvent assisted ionization inlet for mass spectrometry
WO2012058248A2 (en) 2010-10-25 2012-05-03 Wayne State University Systems and methods extending the laserspray ionization mass spectrometry concept from atmospheric pressure to vacuum
US9488625B2 (en) 2010-12-15 2016-11-08 Baxalta GmbH Purification of factor VIII using a conductivity gradient
US8506797B2 (en) * 2011-04-10 2013-08-13 Therapeutic Proteins International, LLC Downstream bioprocessing device
EP2970865B1 (de) * 2013-03-15 2020-09-02 Alder Biopharmaceuticals, Inc. Antikörperreinigung und -reinheitsüberwachung
WO2014195783A1 (en) * 2013-06-05 2014-12-11 Dh Technologies Development Pte. Ltd. Swathtm data-independent acquisition technology for the detection of host cell protein contaminants in biotherapeutics protein products
TW202204596A (zh) * 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
CN109564217A (zh) * 2016-04-14 2019-04-02 隆扎有限公司 用于检测宿主细胞蛋白的组合物和方法
CN109118004B (zh) * 2018-08-16 2021-09-14 李宏伟 一种工程构筑选址适宜区预测方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2163426C (en) * 1993-05-28 2005-11-01 T. William Hutchens Method and apparatus for desorption and ionization of analytes
US20020151707A1 (en) * 1995-06-07 2002-10-17 Corixa Corp. Immune mediators and related methods
US5993665A (en) * 1996-06-07 1999-11-30 Immunivest Corporation Quantitative cell analysis methods employing magnetic separation
ATE209213T1 (de) * 1996-09-09 2001-12-15 Rmf Dictagene Sa Herstellung von gereinigten (poly)peptiden
NZ516848A (en) * 1997-06-20 2004-03-26 Ciphergen Biosystems Inc Retentate chromatography apparatus with applications in biology and medicine
US6207370B1 (en) * 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6210910B1 (en) * 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
WO2000011208A1 (en) * 1998-08-25 2000-03-02 University Of Washington Rapid quantitative analysis of proteins or protein function in complex mixtures
US6558946B1 (en) * 2000-08-29 2003-05-06 The United States Of America As Represented By The Secretary Of The Army Automated sample processing for identification of microorganisms and proteins

Also Published As

Publication number Publication date
CA2465214A1 (en) 2003-05-22
US20030091976A1 (en) 2003-05-15
WO2003042233A2 (en) 2003-05-22
US20070059776A1 (en) 2007-03-15
JP2005509173A (ja) 2005-04-07
EP1448760A2 (de) 2004-08-25
EP1448760A4 (de) 2005-01-05
EP1448760B1 (de) 2008-12-31
AU2002357699A1 (en) 2003-05-26
WO2003042233A3 (en) 2004-01-08
ATE419329T1 (de) 2009-01-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee