ATE384789T1 - Verfahren zur sequenz-saturations-mutagenese (sesam) - Google Patents

Verfahren zur sequenz-saturations-mutagenese (sesam)

Info

Publication number
ATE384789T1
ATE384789T1 AT04787058T AT04787058T ATE384789T1 AT E384789 T1 ATE384789 T1 AT E384789T1 AT 04787058 T AT04787058 T AT 04787058T AT 04787058 T AT04787058 T AT 04787058T AT E384789 T1 ATE384789 T1 AT E384789T1
Authority
AT
Austria
Prior art keywords
strand
terminus
sequence
collection
master sequence
Prior art date
Application number
AT04787058T
Other languages
English (en)
Inventor
Ulrich Schwaneberg
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag filed Critical Basf Ag
Application granted granted Critical
Publication of ATE384789T1 publication Critical patent/ATE384789T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
AT04787058T 2003-10-02 2004-09-30 Verfahren zur sequenz-saturations-mutagenese (sesam) ATE384789T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03022311 2003-10-02

Publications (1)

Publication Number Publication Date
ATE384789T1 true ATE384789T1 (de) 2008-02-15

Family

ID=34429231

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04787058T ATE384789T1 (de) 2003-10-02 2004-09-30 Verfahren zur sequenz-saturations-mutagenese (sesam)

Country Status (9)

Country Link
US (1) US7790374B2 (de)
EP (1) EP1670914B1 (de)
JP (1) JP4299342B2 (de)
CN (1) CN1860227B (de)
AT (1) ATE384789T1 (de)
DE (1) DE602004011546T2 (de)
DK (1) DK1670914T3 (de)
ES (1) ES2297492T3 (de)
WO (1) WO2005035757A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011157573A2 (de) 2010-06-14 2011-12-22 Evonik Röhm Gmbh Ein enzym zur herstellung von methylmalonatsemialdehyd
CN102169118B (zh) * 2011-04-15 2012-01-25 华瑞同康生物技术(深圳)有限公司 血清或尿液中8-氧代脱氧鸟苷的定量检测方法
DE102012017026A1 (de) 2012-08-28 2014-03-06 Forschungszentrum Jülich GmbH Sensor für NADP(H) und Entwicklung von Alkoholdehydrogenasen
CA3048448A1 (en) 2016-12-28 2018-07-05 Invvax, Inc. Influenza vaccines
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
WO2020165137A1 (en) * 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6428955B1 (en) * 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US6238884B1 (en) * 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
GB9600384D0 (en) * 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases
GB9602028D0 (en) * 1996-02-01 1996-04-03 Amersham Int Plc Nucleoside analogues
BRPI0214741B1 (pt) * 2001-12-08 2015-05-12 Seegene Inc Iniciador de controle de anelamento para melhorar a especificidade de anelamento na amplificação de ácido nucléico, Kit, Métodos para amplificar seqüências de ácido nucléico de um DNA e seqüência de ácido nucléico alvo ou uma mistura de ácidos nucléicos, para detectar a complementaridade de DNA ao mRNA diferencialmente expresso em duas ou mais amostras de ácido nucléico, para amplificar rapidamente um fragmento de CDNA alvo, para amplificar uma população de CDNAs de filamento duplo de comprimento completo complementar aos mRNAS, e CDNAs de filamento duplo enriquecidos em 5’ complementares aos mRNAS, para amplificar mais do que uma seqüência de nucleotídeo alvo simultaneamente, para produzir uma impressão digital de DNA de gDNA, e de RNA de uma amostra de mRNA, para identificar segmentos de homologia conservada em uma família de multigene a partir de uma amostra de mRNA, para identificar uma variação de nucleotídeo em um ácido nucléico alvo, para a mutagênese em um ácido nucléico alvo, e, uso do iniciador

Also Published As

Publication number Publication date
EP1670914B1 (de) 2008-01-23
DK1670914T3 (da) 2008-05-13
US7790374B2 (en) 2010-09-07
DE602004011546D1 (de) 2008-03-13
JP4299342B2 (ja) 2009-07-22
DE602004011546T2 (de) 2008-05-29
ES2297492T3 (es) 2008-05-01
CN1860227A (zh) 2006-11-08
CN1860227B (zh) 2012-05-16
EP1670914A1 (de) 2006-06-21
WO2005035757A1 (en) 2005-04-21
JP2007507212A (ja) 2007-03-29
US20060223148A1 (en) 2006-10-05

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