CN1860227B - 用于序列饱和诱变(SeSaM)的方法 - Google Patents
用于序列饱和诱变(SeSaM)的方法 Download PDFInfo
- Publication number
- CN1860227B CN1860227B CN2004800285655A CN200480028565A CN1860227B CN 1860227 B CN1860227 B CN 1860227B CN 2004800285655 A CN2004800285655 A CN 2004800285655A CN 200480028565 A CN200480028565 A CN 200480028565A CN 1860227 B CN1860227 B CN 1860227B
- Authority
- CN
- China
- Prior art keywords
- chain
- polynucleotide sequence
- nucleotide
- double
- produces
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Peptides Or Proteins (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (16)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP03022311.9 | 2003-10-02 | ||
EP03022311 | 2003-10-02 | ||
PCT/EP2004/010911 WO2005035757A1 (en) | 2003-10-02 | 2004-09-30 | A process for sequence saturation mutagenesis (sesam) |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1860227A CN1860227A (zh) | 2006-11-08 |
CN1860227B true CN1860227B (zh) | 2012-05-16 |
Family
ID=34429231
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2004800285655A Expired - Fee Related CN1860227B (zh) | 2003-10-02 | 2004-09-30 | 用于序列饱和诱变(SeSaM)的方法 |
Country Status (9)
Country | Link |
---|---|
US (1) | US7790374B2 (zh) |
EP (1) | EP1670914B1 (zh) |
JP (1) | JP4299342B2 (zh) |
CN (1) | CN1860227B (zh) |
AT (1) | ATE384789T1 (zh) |
DE (1) | DE602004011546T2 (zh) |
DK (1) | DK1670914T3 (zh) |
ES (1) | ES2297492T3 (zh) |
WO (1) | WO2005035757A1 (zh) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011157573A2 (de) | 2010-06-14 | 2011-12-22 | Evonik Röhm Gmbh | Ein enzym zur herstellung von methylmalonatsemialdehyd |
CN102169118B (zh) * | 2011-04-15 | 2012-01-25 | 华瑞同康生物技术(深圳)有限公司 | 血清或尿液中8-氧代脱氧鸟苷的定量检测方法 |
DE102012017026A1 (de) | 2012-08-28 | 2014-03-06 | Forschungszentrum Jülich GmbH | Sensor für NADP(H) und Entwicklung von Alkoholdehydrogenasen |
WO2018126060A1 (en) | 2016-12-28 | 2018-07-05 | Invvax, Inc. | Influenza vaccines |
GB201811810D0 (en) * | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
AU2020223456A1 (en) * | 2019-02-12 | 2021-08-12 | Dna Script | Efficient product cleavage in template-free enzymatic synthesis of polynucleotides. |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6238884B1 (en) * | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6428955B1 (en) * | 1995-03-17 | 2002-08-06 | Sequenom, Inc. | DNA diagnostics based on mass spectrometry |
GB9600384D0 (en) * | 1996-01-09 | 1996-03-13 | Nyfotek As | Dna glycosylases |
GB9602028D0 (en) * | 1996-02-01 | 1996-04-03 | Amersham Int Plc | Nucleoside analogues |
CA2468754C (en) | 2001-12-08 | 2011-11-15 | Seegene, Inc. | Annealing control primer and its uses |
-
2004
- 2004-09-30 EP EP04787058A patent/EP1670914B1/en not_active Not-in-force
- 2004-09-30 WO PCT/EP2004/010911 patent/WO2005035757A1/en active IP Right Grant
- 2004-09-30 DE DE602004011546T patent/DE602004011546T2/de active Active
- 2004-09-30 JP JP2006530045A patent/JP4299342B2/ja not_active Expired - Fee Related
- 2004-09-30 DK DK04787058T patent/DK1670914T3/da active
- 2004-09-30 US US10/573,639 patent/US7790374B2/en not_active Expired - Fee Related
- 2004-09-30 ES ES04787058T patent/ES2297492T3/es active Active
- 2004-09-30 CN CN2004800285655A patent/CN1860227B/zh not_active Expired - Fee Related
- 2004-09-30 AT AT04787058T patent/ATE384789T1/de active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6238884B1 (en) * | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
Non-Patent Citations (2)
Title |
---|
Methods in enzymology 155 0076-6879.Academic press inc.,1987,555-569. * |
Methods in enzymology155 0076-6879.Academic press inc.,1987,555-569. |
Also Published As
Publication number | Publication date |
---|---|
JP2007507212A (ja) | 2007-03-29 |
JP4299342B2 (ja) | 2009-07-22 |
DE602004011546D1 (de) | 2008-03-13 |
EP1670914B1 (en) | 2008-01-23 |
US7790374B2 (en) | 2010-09-07 |
ES2297492T3 (es) | 2008-05-01 |
EP1670914A1 (en) | 2006-06-21 |
ATE384789T1 (de) | 2008-02-15 |
CN1860227A (zh) | 2006-11-08 |
US20060223148A1 (en) | 2006-10-05 |
WO2005035757A1 (en) | 2005-04-21 |
DK1670914T3 (da) | 2008-05-13 |
DE602004011546T2 (de) | 2008-05-29 |
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Legal Events
Date | Code | Title | Description |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: JACOBS UNIVERSITY BREMEN CO., LTD. Owner name: SATURATION MUTAGENESIS-BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: BASF SE Effective date: 20100825 |
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C41 | Transfer of patent application or patent right or utility model | ||
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: LUDWIGSHAFEN, GERMANY TO: BREMEN, GERMANY |
|
TA01 | Transfer of patent application right |
Effective date of registration: 20100825 Address after: Bremen Applicant after: BASF AG Co-applicant after: Bremen Jacobs university Address before: Ludwigshafen, Germany Applicant before: BASF SE |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120516 Termination date: 20190930 |