ATE356221T1 - Sequenzierung durch ligation kodierter adapter - Google Patents

Sequenzierung durch ligation kodierter adapter

Info

Publication number
ATE356221T1
ATE356221T1 AT97929757T AT97929757T ATE356221T1 AT E356221 T1 ATE356221 T1 AT E356221T1 AT 97929757 T AT97929757 T AT 97929757T AT 97929757 T AT97929757 T AT 97929757T AT E356221 T1 ATE356221 T1 AT E356221T1
Authority
AT
Austria
Prior art keywords
encoded
ligation
protruding strands
ligated
target polynucleotide
Prior art date
Application number
AT97929757T
Other languages
German (de)
English (en)
Inventor
Glenn Albrecht
Sydney Brenner
David H Lloyd
Robert B Dubridge
Michael C Pallas
Original Assignee
Solexa Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/659,453 external-priority patent/US5846719A/en
Application filed by Solexa Inc filed Critical Solexa Inc
Application granted granted Critical
Publication of ATE356221T1 publication Critical patent/ATE356221T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
AT97929757T 1996-06-06 1997-06-02 Sequenzierung durch ligation kodierter adapter ATE356221T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/659,453 US5846719A (en) 1994-10-13 1996-06-06 Oligonucleotide tags for sorting and identification
US68958796A 1996-08-12 1996-08-12

Publications (1)

Publication Number Publication Date
ATE356221T1 true ATE356221T1 (de) 2007-03-15

Family

ID=27097828

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97929757T ATE356221T1 (de) 1996-06-06 1997-06-02 Sequenzierung durch ligation kodierter adapter

Country Status (12)

Country Link
EP (1) EP0923650B1 (enExample)
JP (1) JP4124377B2 (enExample)
CN (1) CN1195872C (enExample)
AT (1) ATE356221T1 (enExample)
AU (1) AU733782B2 (enExample)
CA (1) CA2256700A1 (enExample)
CZ (1) CZ397998A3 (enExample)
DE (1) DE69737450T2 (enExample)
HU (1) HUP0003944A3 (enExample)
NO (1) NO985698L (enExample)
PL (1) PL331513A1 (enExample)
WO (1) WO1997046704A1 (enExample)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
AU718357B2 (en) * 1995-06-07 2000-04-13 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US5888737A (en) * 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
EP0985142A4 (en) 1997-05-23 2006-09-13 Lynx Therapeutics Inc SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES
US6607878B2 (en) * 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
JP3944996B2 (ja) 1998-03-05 2007-07-18 株式会社日立製作所 Dnaプローブアレー
WO1999067641A2 (en) 1998-06-24 1999-12-29 Illumina, Inc. Decoding of array sensors with microspheres
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
WO2000039333A1 (en) * 1998-12-23 2000-07-06 Jones Elizabeth Louise Sequencing method using magnifying tags
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP1190092A2 (en) 1999-04-06 2002-03-27 Yale University Fixed address analysis of sequence tags
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
WO2000075373A2 (en) 1999-05-20 2000-12-14 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
WO2001012855A2 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
US7604996B1 (en) 1999-08-18 2009-10-20 Illumina, Inc. Compositions and methods for preparing oligonucleotide solutions
DK1259643T3 (da) 2000-02-07 2009-02-23 Illumina Inc Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming
AU2001238067B2 (en) 2000-02-07 2007-01-25 Illumina, Inc. Nucleic acid detection methods using universal priming
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7361488B2 (en) 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
AU3838901A (en) 2000-02-16 2001-08-27 Illumina Inc Parallel genotyping of multiple patient samples
US7291460B2 (en) * 2002-05-31 2007-11-06 Verenium Corporation Multiplexed systems for nucleic acid sequencing
CA2513985C (en) 2003-01-21 2012-05-29 Illumina Inc. Chemical reaction monitor
ES2396245T3 (es) * 2003-01-29 2013-02-20 454 Life Sciences Corporation Método de amplificación y secuenciamiento de ácidos nucleicos
GB0308852D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
WO2005010184A1 (ja) * 2003-07-25 2005-02-03 Takara Bio Inc. 変異の検出方法
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
KR20070112785A (ko) 2005-02-01 2007-11-27 에이젠코트 바이오사이언스 코오포레이션 비드-기초 서열화를 위한 시약, 방법, 및 라이브러리
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
SG169356A1 (en) * 2006-02-08 2011-03-30 Illumina Cambridge Ltd Method for sequencing a polynucleotide template
WO2007097443A1 (ja) * 2006-02-20 2007-08-30 National University Corporation Hokkaido University Dna塩基配列を決定する方法
WO2007121489A2 (en) * 2006-04-19 2007-10-25 Applera Corporation Reagents, methods, and libraries for gel-free bead-based sequencing
CN101495656B (zh) 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8845880B2 (en) 2010-12-22 2014-09-30 Genia Technologies, Inc. Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
EP3425063B9 (en) 2011-12-22 2023-10-04 President and Fellows of Harvard College Methods for analyte detection
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
CN102766689B (zh) * 2012-04-17 2015-07-22 盛司潼 一种增加测序读长的测序方法
LT2841601T (lt) * 2012-04-24 2019-07-10 Gen9, Inc. Nukleorūgščių rūšiavimo būdai ir multipleksinis preparatyvinis in vitro klonavimas
CA2873176C (en) * 2012-05-10 2024-02-27 The General Hospital Corporation Methods for determining a nucleotide sequence
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9494554B2 (en) 2012-06-15 2016-11-15 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
RU2756865C2 (ru) 2013-06-04 2021-10-06 Президент Энд Фэллоуз Оф Харвард Коллидж Направляемая рнк регуляция транскрипции
EP3052645B1 (en) * 2013-09-30 2019-01-09 Qiagen GmbH Dna-adapter-molecules for the preparation of dna-libraries and method for producing them and use
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
JP6461943B2 (ja) 2013-10-23 2019-01-30 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアを備えた高速分子検知
KR102320074B1 (ko) * 2014-01-28 2021-11-03 란자테크 뉴질랜드 리미티드 재조합 미생물의 생산 방법
WO2015117040A1 (en) * 2014-01-31 2015-08-06 Swift Biosciences, Inc. Improved methods for processing dna substrates
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP3842544A1 (en) * 2014-09-05 2021-06-30 QIAGEN GmbH Preparation of adapter-ligated amplicons
DK3192869T3 (da) * 2014-09-12 2019-05-20 Mgi Tech Co Ltd Isoleret oligonukleotid og anvendelse deraf i nukleinsyresekvensering
WO2016058517A1 (en) * 2014-10-14 2016-04-21 Bgi Shenzhen Co., Limited Mate pair library construction
WO2016058136A1 (zh) * 2014-10-14 2016-04-21 深圳华大基因科技有限公司 一种接头元件和使用其构建测序文库的方法
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
CN106435744B (zh) * 2015-08-13 2022-02-01 生捷科技控股公司 使用y-接头和消失限制位点的文库构建
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
EP3390658B1 (en) 2015-12-16 2022-08-03 Standard BioTools Inc. High-level multiplex amplification
US11680253B2 (en) 2016-03-10 2023-06-20 The Board Of Trustees Of The Leland Stanford Junior University Transposase-mediated imaging of the accessible genome
AU2017257624B2 (en) 2016-04-25 2023-05-25 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
AU2017295717B2 (en) * 2016-07-15 2021-06-24 The Regents Of The University Of California Methods of producing nucleic acid libraries
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
CN109983125B (zh) 2016-08-31 2024-06-04 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
US10550425B2 (en) 2016-11-14 2020-02-04 Agilent Technologies, Inc. Composition and method for improving signal-to-noise in hybridization to oligonucleotide arrays
WO2018102064A1 (en) 2016-11-30 2018-06-07 Microsoft Technology Licensing, Llc. Dna random access storage system via ligation
US11185568B2 (en) 2017-04-14 2021-11-30 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
CN107299151B (zh) * 2017-08-28 2020-06-09 华中农业大学 用于鉴别稻米脂肪酸品质的dna分子标记及其应用
US11584929B2 (en) 2018-01-12 2023-02-21 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
CN119020348A (zh) 2018-02-05 2024-11-26 斯坦福大学托管董事会 用于单细胞和集合细胞的多重测量的系统和方法
JP7537748B2 (ja) 2018-06-06 2024-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5650274A (en) * 1993-06-25 1997-07-22 Hitachi, Ltd. DNA analyzing method
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
WO1996041011A1 (en) * 1995-06-07 1996-12-19 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification

Also Published As

Publication number Publication date
DE69737450D1 (de) 2007-04-19
JP4124377B2 (ja) 2008-07-23
NO985698D0 (no) 1998-12-04
EP0923650A1 (en) 1999-06-23
HK1021206A1 (en) 2000-06-02
JP2000515006A (ja) 2000-11-14
EP0923650B1 (en) 2007-03-07
AU3374097A (en) 1998-01-05
CN1230226A (zh) 1999-09-29
WO1997046704A1 (en) 1997-12-11
DE69737450T2 (de) 2007-11-29
CA2256700A1 (en) 1997-12-11
NO985698L (no) 1999-02-08
CZ397998A3 (cs) 1999-07-14
AU733782B2 (en) 2001-05-24
PL331513A1 (en) 1999-07-19
CN1195872C (zh) 2005-04-06
HUP0003944A3 (en) 2003-08-28
HUP0003944A2 (en) 2001-03-28

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