ATE338127T1 - Ribonukleaseresistente rna präparation und seine verwendung - Google Patents

Ribonukleaseresistente rna präparation und seine verwendung

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Publication number
ATE338127T1
ATE338127T1 AT97933547T AT97933547T ATE338127T1 AT E338127 T1 ATE338127 T1 AT E338127T1 AT 97933547 T AT97933547 T AT 97933547T AT 97933547 T AT97933547 T AT 97933547T AT E338127 T1 ATE338127 T1 AT E338127T1
Authority
AT
Austria
Prior art keywords
rna preparation
ribonuclease resistant
resistant rna
ribonuclease
nucleic acids
Prior art date
Application number
AT97933547T
Other languages
German (de)
English (en)
Inventor
Dwight B Dubois
Matthew M Winkler
Brittan L Pasloske
Original Assignee
Ambion Inc
Cenetron Diagnostics Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/675,153 external-priority patent/US5677124A/en
Application filed by Ambion Inc, Cenetron Diagnostics Llc filed Critical Ambion Inc
Application granted granted Critical
Publication of ATE338127T1 publication Critical patent/ATE338127T1/de

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    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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AT97933547T 1996-07-03 1997-07-02 Ribonukleaseresistente rna präparation und seine verwendung ATE338127T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2114596P 1996-07-03 1996-07-03
US08/675,153 US5677124A (en) 1996-07-03 1996-07-03 Ribonuclease resistant viral RNA standards
US08/881,571 US5939262A (en) 1996-07-03 1997-06-24 Ribonuclease resistant RNA preparation and utilization

Publications (1)

Publication Number Publication Date
ATE338127T1 true ATE338127T1 (de) 2006-09-15

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ID=26694321

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97933547T ATE338127T1 (de) 1996-07-03 1997-07-02 Ribonukleaseresistente rna präparation und seine verwendung

Country Status (8)

Country Link
US (5) US5939262A (enExample)
EP (1) EP0910643B1 (enExample)
JP (2) JP4315471B2 (enExample)
AT (1) ATE338127T1 (enExample)
AU (1) AU729601B2 (enExample)
CA (1) CA2258485C (enExample)
DE (1) DE69736598T2 (enExample)
WO (1) WO1998000547A1 (enExample)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
DE19737442C2 (de) * 1997-08-22 1999-06-24 Olfert Landt Tib Molbiol Synth Proteinumhüllte Polyribonukleinsäuren, ein Verfahren zu ihrer Herstellung und ihre Verwendung
AU9319398A (en) * 1997-09-19 1999-04-05 Sequitur, Inc. Sense mrna therapy
EP1892299A3 (en) * 1997-09-19 2011-07-20 Life Technologies Corporation Sense mRNA therapy
US7767422B2 (en) * 1998-09-22 2010-08-03 Oncomedx, Inc. Detection of 5T4 RNA in plasma and serum
US6777210B1 (en) 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
DE19956568A1 (de) * 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
KR20000065690A (ko) * 1999-04-08 2000-11-15 박종구 반응 특이성 및 안정성을 개선시킨 안티센스 올리고 뉴클레오타이드, 안티센스 dna 및 그 제조방법
US7829693B2 (en) * 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
EP1268766B1 (en) * 2000-03-31 2005-11-30 Cambridge Antibody Technology Limited Improvements to ribosome display
DE60144027D1 (de) * 2000-08-28 2011-03-24 Oncomedx Inc 5t4 rna in plasma und serum als marker für neoplastische erkrankungen
GB0021303D0 (en) * 2000-08-30 2000-10-18 Medinnova Sf Use
RU2189396C2 (ru) * 2000-10-27 2002-09-20 Федеральное государственное учреждение науки и здравоохранения Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им. акад. И.Н.Блохиной Способ обнаружения и дифференциации рнк энтеровирусов
US8546143B2 (en) 2001-01-09 2013-10-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US20020172972A1 (en) * 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
AU2002351221A1 (en) * 2001-12-06 2003-06-23 The Aaron Diamond Aids Research Center Highly-sensitive genomic assays employing chimeric bacteriophage standards
WO2003064605A2 (en) 2002-01-28 2003-08-07 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
EP2455467B1 (en) 2002-02-01 2016-10-05 Life Technologies Corporation Double-stranded oligonucleotides
WO2003103605A2 (en) * 2002-06-07 2003-12-18 Large Scale Biology Corporation Flexible vaccine assembly and vaccine delivery platform
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
WO2004085611A2 (en) * 2003-03-21 2004-10-07 The Research Foundation Of State University Of New York Model for mutually exclusive domain folding molecular switch
BRPI0410780A (pt) * 2003-05-20 2006-06-27 Investigen Inc sistema para detectar polinucleotìdeos
EP2371835A1 (en) 2003-07-03 2011-10-05 The Trustees Of The University Of Pennsylvania Inhibition of syk kinase expression
JP4435787B2 (ja) * 2003-12-16 2010-03-24 キアジェン ノース アメリカン ホールディングス,インコーポレイティド タンパク質を変性させるための処方物および方法
WO2005097817A2 (en) 2004-04-05 2005-10-20 Alnylam Pharmaceuticals, Inc. Process and reagents for oligonucleotide synthesis and purification
US7626014B2 (en) 2004-04-27 2009-12-01 Alnylam Pharmaceuticals Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety
EP3034510A1 (en) * 2004-04-30 2016-06-22 Alnylam Pharmaceuticals Inc. Oligonucleotides comprising a c5-modified pyrimidine
EP1789553B1 (en) * 2004-06-30 2014-03-26 Alnylam Pharmaceuticals Inc. Oligonucleotides comprising a non-phosphate backbone linkage
CA2574088C (en) 2004-07-21 2013-09-17 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a modified or non-natural nucleobase
WO2006112872A2 (en) 2004-08-04 2006-10-26 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase
US7309589B2 (en) 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
AU2005335203A1 (en) * 2004-09-08 2007-02-15 The United States Of America As Represented By The Secretary Of Health And Human Services, Nih Compositions and methods for the detection of HIV-1/HIV-2 infection
WO2006045591A2 (en) * 2004-10-25 2006-05-04 Devgen N.V. Method and constructs for delivering double stranded rna to pest organisms
AU2005305012C1 (en) 2004-11-05 2012-07-19 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
AU2006270063A1 (en) * 2005-07-15 2007-01-25 Applied Biosystems, Llc. Methods for RNA profiling
US8021839B2 (en) * 2006-02-24 2011-09-20 Investigen, Inc. Methods and compositions for detecting polynucleotides
US20100099149A1 (en) 2006-10-06 2010-04-22 Dna Genotek Inc. Stabilizing compositions and methods for extraction of ribonucleic acid
WO2008051817A1 (en) * 2006-10-20 2008-05-02 Novartis Ag Compositions and method for monitoring bcr-abl expression
US20080096193A1 (en) * 2006-10-24 2008-04-24 Charles Robert Bupp Methods and compositions for detecting polynucleotides
CN101173252B (zh) * 2006-10-31 2011-09-21 杭州市疾病预防控制中心 披甲rna及其制备方法
US7771947B2 (en) * 2007-02-23 2010-08-10 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
EP2268801A4 (en) * 2008-03-14 2011-12-28 Us Gov Health & Human Serv COMPOSITIONS AND METHODS RELATED TO A HUMAN BOCAVIRUS
US8324149B2 (en) * 2008-11-18 2012-12-04 The Board Of Trustees Of The Leland Stanford Junior University Encapsidation of heterologous entities into virus-like particles
US20100129902A1 (en) 2008-11-24 2010-05-27 Erhard Ralf Schoenbrunner Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR)
EP2955233A1 (en) * 2008-12-19 2015-12-16 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor
ES2605303T3 (es) * 2010-07-29 2017-03-13 F. Hoffmann-La Roche Ag Detección cualitativa y cuantitativa de ácidos nucleicos microbianos
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
ES2640522T3 (es) 2010-07-29 2017-11-03 F. Hoffmann-La Roche Ag Ácidos nucleicos de control para múltiples parámetros
CA2802563C (en) 2010-07-29 2018-08-28 F. Hoffmann-La Roche Ag Preparation of nucleic acids from different types of biological fluid samples
JP6121591B2 (ja) * 2010-07-29 2017-04-26 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 微生物核酸の定性的および定量的検出
EP3578205A1 (en) 2010-08-06 2019-12-11 ModernaTX, Inc. A pharmaceutical formulation comprising engineered nucleic acids and medical use thereof
EP2426222A1 (en) 2010-09-07 2012-03-07 F. Hoffmann-La Roche AG Generic buffer for amplification
CN103429606A (zh) 2010-10-01 2013-12-04 现代治疗公司 设计核酸及其使用方法
EP2465945A1 (en) 2010-12-17 2012-06-20 F. Hoffmann-La Roche AG Generic matrix for control nucleic acids
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
EP2721140B1 (en) 2011-06-19 2016-11-23 Abogen, Inc. Devices, solutions and methods for sample collection
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
KR20190099538A (ko) 2011-10-03 2019-08-27 모더나 세라퓨틱스, 인코포레이티드 변형된 뉴클레오사이드, 뉴클레오타이드, 및 핵산, 및 이들의 용도
EP4144378A1 (en) 2011-12-16 2023-03-08 ModernaTX, Inc. Modified nucleoside, nucleotide, and nucleic acid compositions
SG10201504415YA (en) 2012-03-13 2015-07-30 Emd Millipore Corp Use of centrifugation-aided infection to increase virus titer
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
AU2013243954A1 (en) 2012-04-02 2014-10-30 Moderna Therapeutics, Inc. Modified polynucleotides for the production of cosmetic proteins and peptides
DK2922554T3 (en) 2012-11-26 2022-05-23 Modernatx Inc Terminalt modificeret rna
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
CN105378060B (zh) 2013-03-13 2019-12-10 经纬生物科技有限公司 非复制型转导颗粒和基于转导颗粒的报告系统
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
US10023626B2 (en) 2013-09-30 2018-07-17 Modernatx, Inc. Polynucleotides encoding immune modulating polypeptides
BR112016007255A2 (pt) 2013-10-03 2017-09-12 Moderna Therapeutics Inc polinucleotídeos que codificam receptor de lipoproteína de baixa densidade
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
US20160355896A1 (en) * 2014-02-10 2016-12-08 Amptec Gmbh Incoated rna
PT3981437T (pt) 2014-04-23 2025-01-15 Modernatx Inc Vacinas de ácidos nucleicos
GB201412316D0 (en) 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
US11015217B2 (en) 2015-05-29 2021-05-25 Roche Molecular Systems, Inc. Method of inactivating microbes by citraconic anhydride
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells
WO2017072303A1 (en) 2015-10-28 2017-05-04 Life Science Inkubator Gmbh Use of vlp for the detection of nucleic acids
US10689689B2 (en) * 2015-12-28 2020-06-23 Roche Molecular Systems, Inc. Generic method for the stabilization of specific RNA
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
EP3299471B1 (en) 2016-09-23 2019-10-23 Roche Diagniostics GmbH Methods for determining the amount of a nucleic acid of interest in an unprocessed sample
US10422012B2 (en) 2016-10-10 2019-09-24 Roche Molecular Systems, Inc. Devices comprising bacteriophage PHI6 internal control compositions
US11021763B2 (en) 2017-10-03 2021-06-01 Abbott Molecular Inc. Assay for detecting hepatitis C virus (HCV)
CA3068498A1 (en) 2017-10-03 2019-04-11 Abbott Molecular Inc. Assay for detecting human immunodeficiency virus (hiv)
US11015228B2 (en) 2018-01-09 2021-05-25 Abbott Molecular Inc. Assay for detecting Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, and Mycoplasma genitalium
CN115896243A (zh) * 2022-11-25 2023-04-04 上海厦维医学检验实验室有限公司 基于混合酶体系的超灵敏反转录方法及其应用

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4866167A (en) * 1985-03-01 1989-09-12 Biotechnica Diagnostics, Inc. Detection of human oral cells by nucleic acid hybridization
CA1288073C (en) * 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US5091309A (en) * 1986-01-16 1992-02-25 Washington University Sindbis virus vectors
GB8608850D0 (en) * 1986-04-11 1986-05-14 Diatech Ltd Packaging system
EP0278667B1 (en) * 1987-02-09 1994-07-20 Mycogen Plant Science, Inc. Hybrid RNA virus
US5316931A (en) * 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5166057A (en) * 1989-08-28 1992-11-24 The Mount Sinai School Of Medicine Of The City University Of New York Recombinant negative strand rna virus expression-systems
SE9003978D0 (sv) * 1990-12-13 1990-12-13 Henrik Garoff Dna expressionssystem baserade paa ett virus replikon
ES2171398T3 (es) * 1991-08-01 2002-09-16 Large Scale Biology Corp Acidos nucleicos viricos vegetales recombinantes.
ATE257860T1 (de) * 1991-08-02 2004-01-15 Biomerieux Bv Quantifikation von nukleinsäuren
DE69333529T2 (de) * 1992-03-31 2004-10-14 Kanebo, Ltd. Pflanzenvirus-vektor, plasmid, methode der expression fremder gene und methode zur gewinnung obiger genprodukte
US5443969A (en) * 1992-10-29 1995-08-22 Rutgers University RNA packaging system
GB9311386D0 (en) * 1993-06-02 1993-07-21 Pna Diagnostics As Nucleic acid analogue assay procedures
US5614413A (en) * 1993-07-01 1997-03-25 The Uab Research Foundation Encapsidated recombinant poliovirus nucleic acid and methods of making and using same
CA2125344A1 (en) * 1993-07-01 1995-01-02 Casey D. Morrow Encapsidated recombinant poliovirus nucleic acid and methods of making and using same
JP3594598B2 (ja) * 1993-07-09 2004-12-02 アクゾ・ノベル・エヌ・ベー 改良された核酸定量方法
WO1995014109A1 (en) * 1993-11-19 1995-05-26 U.S. Department Of The Army High through-put quantitative polymerase chain reaction for hiv clinical specimens
GB2285445A (en) * 1993-12-06 1995-07-12 Pna Diagnostics As Protecting nucleic acids and methods of analysis
US5824507A (en) * 1994-05-20 1998-10-20 Genelabs Technologies, Inc. Hepatitis G virus and molecular cloning thereof
WO1995034684A1 (en) * 1994-06-10 1995-12-21 Georgetown University Recombinant virus and related method of quantitating same
US6613749B1 (en) * 1994-07-26 2003-09-02 Imperial College Innovations Limited Papovavirus pseudocapsids and use thereof for exogenous material transfer
US5716821A (en) * 1994-09-30 1998-02-10 Uab Research Foundation Prevention and treatment of respiratory tract disease
US5736388A (en) * 1994-12-30 1998-04-07 Chada; Sunil Bacteriophage-mediated gene transfer systems capable of transfecting eukaryotic cells
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
AU4657400A (en) 1999-04-20 2000-11-02 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Novel diagnostic standards for virus detection and quantification

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CA2258485A1 (en) 1998-01-08
US20060257889A1 (en) 2006-11-16
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CA2258485C (en) 2013-01-29
DE69736598T2 (de) 2007-08-09
US7033749B2 (en) 2006-04-25
US20020192689A1 (en) 2002-12-19
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