ATE265040T1 - Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen - Google Patents

Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen

Info

Publication number
ATE265040T1
ATE265040T1 AT00915860T AT00915860T ATE265040T1 AT E265040 T1 ATE265040 T1 AT E265040T1 AT 00915860 T AT00915860 T AT 00915860T AT 00915860 T AT00915860 T AT 00915860T AT E265040 T1 ATE265040 T1 AT E265040T1
Authority
AT
Austria
Prior art keywords
applications
capture probe
devices
purification devices
immobilized capture
Prior art date
Application number
AT00915860T
Other languages
English (en)
Inventor
Christopher P Adams
T Christian Boles
Lawrence Weir
Rahul K Dhanda
Stephen J Kron
Original Assignee
Exact Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exact Sciences Corp filed Critical Exact Sciences Corp
Application granted granted Critical
Publication of ATE265040T1 publication Critical patent/ATE265040T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N2001/4038—Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AT00915860T 1999-02-26 2000-02-25 Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen ATE265040T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12183699P 1999-02-26 1999-02-26
PCT/US2000/004771 WO2000050870A1 (en) 1999-02-26 2000-02-25 Biochemical purification devices with immobilized capture probes and their uses

Publications (1)

Publication Number Publication Date
ATE265040T1 true ATE265040T1 (de) 2004-05-15

Family

ID=22399095

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00915860T ATE265040T1 (de) 1999-02-26 2000-02-25 Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen

Country Status (10)

Country Link
US (2) US20020119480A1 (de)
EP (1) EP1157264B1 (de)
JP (1) JP2002537781A (de)
AT (1) ATE265040T1 (de)
AU (1) AU772213C (de)
CA (1) CA2369083A1 (de)
DE (1) DE60010058T2 (de)
DK (1) DK1157264T3 (de)
ES (1) ES2221841T3 (de)
WO (1) WO2000050870A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2289925A1 (en) 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
AU762049B2 (en) * 1998-06-19 2003-06-19 Exact Sciences Corporation Detection of non-viral organisms with SRP RNA
JP4794052B2 (ja) 1999-04-09 2011-10-12 ジェンザイム・コーポレーション 癌の指標である核酸を検出するための方法
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
WO2004061085A2 (en) 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
WO2004065543A2 (en) * 2003-01-16 2004-08-05 Exact Sciences Corporation Devices and method for recovery of a target molecule from a sample
WO2005047881A2 (en) * 2003-11-05 2005-05-26 Exact Sciences Corporation Repetitive reversed-field affinity electrophoresis and uses therefor
ATE515325T1 (de) * 2003-12-12 2011-07-15 Becton Dickinson Co Verfahren zur befestigung einer membran mittels heissschmelzen ohne klebstoff
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
WO2006032044A2 (en) 2004-09-15 2006-03-23 Microchip Biotechnologies, Inc. Microfluidic devices
US7666593B2 (en) * 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
CA2641271A1 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US7766033B2 (en) * 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US20100056388A1 (en) * 2006-08-21 2010-03-04 Cnvgenes, Inc. Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
US8841116B2 (en) * 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
DE102006050871B4 (de) * 2006-10-27 2011-06-01 Albert-Ludwigs-Universität Freiburg Integriertes mikrofluidisches Bauteil zum Aufreinigen von Analytmolekülen sowie Verfahren zum Aufreinigen
KR20100028526A (ko) 2007-02-05 2010-03-12 마이크로칩 바이오테크놀로지스, 인크. 마이크로유체 및 나노유체 장치, 시스템 및 응용
US8568580B2 (en) 2007-07-24 2013-10-29 Applied Biosystems, Llc Systems and methods for isolating nucleic acids
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
EP2384429A1 (de) 2008-12-31 2011-11-09 Integenx Inc. Instrument mit mikrofluidischem chip
CN102459565A (zh) 2009-06-02 2012-05-16 尹特根埃克斯有限公司 具有隔膜阀的流控设备
EP2438016B1 (de) 2009-06-05 2021-06-02 IntegenX Inc. Universelles probenpräparationssystem und seine verwendung in einem integrierten analysesystem
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
EP2606242A4 (de) 2010-08-20 2016-07-20 Integenx Inc Mikrofluidikvorrichtungen mit mechanisch abgedichteten membranventilen
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
CN105873681B (zh) 2013-11-18 2019-10-11 尹特根埃克斯有限公司 用于样本分析的卡盒和仪器
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
CN113092563B (zh) 2014-10-22 2024-06-07 尹特根埃克斯有限公司 用于样品制备、处理和分析的系统和方法
WO2016193282A1 (en) * 2015-06-01 2016-12-08 Qiagen Gmbh Electrophoresis assisted method for purifying a target nucleic acid using a delayed elution approach
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4829098A (en) * 1986-06-19 1989-05-09 Washington Research Foundation Immobilized biomolecules and method of making same
US5034428A (en) * 1986-06-19 1991-07-23 Board Of Regents Of The University Of Washington Immobilized biomolecules and method of making same
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
US5006473A (en) * 1988-08-09 1991-04-09 Abbott Laboratories Electrophoresis method using vesicles
JPH0347097A (ja) * 1989-07-13 1991-02-28 Hitachi Ltd ハイブリダイゼーション方法、これを用いた遺伝子変異検出方法及びその装置
US5346672A (en) * 1989-11-17 1994-09-13 Gene Tec Corporation Devices for containing biological specimens for thermal processing
US5215882A (en) * 1989-11-30 1993-06-01 Ortho Diagnostic Systems, Inc. Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
US5176966A (en) * 1990-11-19 1993-01-05 Ballard Power Systems Inc. Fuel cell membrane electrode and seal assembly
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5762876A (en) * 1991-03-05 1998-06-09 Molecular Tool, Inc. Automatic genotype determination
US5453382A (en) * 1991-08-05 1995-09-26 Indiana University Foundation Electrochromatographic preconcentration method
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
AU675251B2 (en) * 1993-01-04 1997-01-30 Becton Dickinson & Company Flow-through hybridization assay for oligonucleotide sequences
US5482836A (en) * 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
JPH0751099A (ja) * 1993-08-11 1995-02-28 Toyobo Co Ltd 核酸配列検査方法及び検査装置
ATE266206T1 (de) * 1993-09-17 2004-05-15 Hoffmann La Roche Analysengerät mit einer vorrichtung zur suspension von partikeln und ein verfahren zur durchführung der suspension
EP0754240B1 (de) * 1994-02-07 2003-08-20 Beckman Coulter, Inc. Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse
DE69422604T2 (de) * 1994-03-08 2000-06-08 Zeptosens Ag, Witterwil Vorrichtung und Verfahren, die Bioaffinitätsassay und elektrophoretische Auftrennung kombinieren
KR0113205Y1 (ko) * 1994-05-19 1998-04-17 구자홍 자기기록재생기의 경사포스트 베이스 안착장치
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6045995A (en) * 1994-08-24 2000-04-04 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
JP3337386B2 (ja) * 1995-12-08 2002-10-21 理化学研究所 Ｄｎａシーケンスサンプルの精製及び分離検出系への移行方法並びにそれに用いるプレート
US5856100A (en) * 1995-12-08 1999-01-05 The Institute Of Physical And Chemical Research Method for purification and transfer to separation/detection systems of DNA sequencing samples and plates used therefor
US5804384A (en) * 1996-12-06 1998-09-08 Vysis, Inc. Devices and methods for detecting multiple analytes in samples
DE19702907A1 (de) * 1997-01-28 1998-07-30 Boehringer Mannheim Gmbh Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren
US5932711A (en) * 1997-03-05 1999-08-03 Mosaic Technologies, Inc. Nucleic acid-containing polymerizable complex
DE19751968A1 (de) * 1997-11-24 1999-07-29 Lwg Lausitzer Wasser Gmbh & Co Verfahren und Vorrichtung zur Mikroextraktion mit Hilfe einer porösen Glasfaser
US6387632B2 (en) * 1998-06-11 2002-05-14 Hitachi, Ltd. Polynucleotide separation method and apparatus therefor
IL136379A0 (en) * 2000-05-25 2001-06-14 Gene Bio Applic Ltd Processing chamber

Also Published As

Publication number Publication date
DE60010058T2 (de) 2005-04-28
AU772213C (en) 2004-12-09
WO2000050870A9 (en) 2001-11-22
AU3706300A (en) 2000-09-14
JP2002537781A (ja) 2002-11-12
US20020119480A1 (en) 2002-08-29
AU772213B2 (en) 2004-04-22
ES2221841T3 (es) 2005-01-16
DE60010058D1 (de) 2004-05-27
EP1157264A1 (de) 2001-11-28
US20060088867A1 (en) 2006-04-27
DK1157264T3 (da) 2004-08-16
EP1157264B1 (de) 2004-04-21
CA2369083A1 (en) 2000-08-31
WO2000050870A1 (en) 2000-08-31

Similar Documents

Publication Publication Date Title
ATE265040T1 (de) Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen
EP1411340A3 (de) Biochemische Reinigungsvorrichtungen mit immobilisierten Fang-Sonden und ihre Verwendungszwecke
DK1159453T3 (da) Fremgangsmåde til direkte nukleinsyresekventering
ATE454469T1 (de) Für den nachweis von nukleinsäuren kompetente biologische rohderivate
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
ATE495436T1 (de) Verfahren zur isolierung und markierung von probenmolekülen
DE60136335D1 (de) Parallele genotypisierung mehrerer patientenproben
AU2003291893A1 (en) Biological reagents and methods to verify the efficiency of sample preparation and nucleic acid amplification and/or detection
WO2002042496A3 (en) Methods and devices for characterizing duplex nucleic acid molecules
ATE414096T1 (de) Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen
ATE80666T1 (de) Hybridisierungstest fuer nukleinsaeure.
DE60235005D1 (de) Nachweis der instabilität von mikrosatelliten und
ES2052607T3 (es) Metodos y kits para detectar secuencias de acidos nucleicos.
DE69737628D1 (de) Nukleinsäureprimer und -sonden zur detektion onkogener humaner papillomaviren
DE60108337D1 (de) Methode und testsatz zur detektion von schistosomiasis mittels polymerasekettenreaktion
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
DE69833660D1 (de) Nukleinsäuresonden zur detektion von candida-spezies
WO2001073126A3 (en) Diagnostic devices containing porous material
WO2005054516A3 (en) Peptide nucleic acid probes for analysis of certain staphylococcus species
ATE487938T1 (de) Konzentration und aufreinigung von nukleinsäureproben
ATE90971T1 (de) Methode zur analyse einer spezifischen sequenz von dns oder rns, reagenzien und kits dafuer.
ATE340998T1 (de) Verfahren zur bestimmung von flüchtigen substanzen in lösung
DE50214861D1 (de) Verfahren zum erfassen von nukleinsäuremolekülen mittels mindestens einer einheit zum immobilisieren von nukleinsäuremolekülen und biosensor zum erfassen von nukleinsäuremolekülen
FR2805545B1 (fr) Procede electrochimique de detection d'acides nucleiques
DE69019770D1 (de) Verfahren zur Reinigung, Vervielfachung und zum Nachweis einer Nukleinsäure.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1157264

Country of ref document: EP

REN Ceased due to non-payment of the annual fee