ATE258598T1 - Verfahren zur proteinrückfaltung durch verwendung zwitterionischer wirkstoffe von niedrigem molekulargewicht - Google Patents

Verfahren zur proteinrückfaltung durch verwendung zwitterionischer wirkstoffe von niedrigem molekulargewicht

Info

Publication number
ATE258598T1
ATE258598T1 AT98943346T AT98943346T ATE258598T1 AT E258598 T1 ATE258598 T1 AT E258598T1 AT 98943346 T AT98943346 T AT 98943346T AT 98943346 T AT98943346 T AT 98943346T AT E258598 T1 ATE258598 T1 AT E258598T1
Authority
AT
Austria
Prior art keywords
molecular weight
low molecular
protein refolding
refolding method
zwitterionic agents
Prior art date
Application number
AT98943346T
Other languages
English (en)
Inventor
Steven M Vicik
Original Assignee
Inst Genetics Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Genetics Llc filed Critical Inst Genetics Llc
Application granted granted Critical
Publication of ATE258598T1 publication Critical patent/ATE258598T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT98943346T 1997-10-08 1998-08-24 Verfahren zur proteinrückfaltung durch verwendung zwitterionischer wirkstoffe von niedrigem molekulargewicht ATE258598T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/946,839 US20020052026A1 (en) 1997-10-08 1997-10-08 Methods of refolding proteins
PCT/US1998/017499 WO1999018196A1 (en) 1997-10-08 1998-08-24 Methods of refolding proteins by use of zwitterionic low molecular weight agents

Publications (1)

Publication Number Publication Date
ATE258598T1 true ATE258598T1 (de) 2004-02-15

Family

ID=25485053

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98943346T ATE258598T1 (de) 1997-10-08 1998-08-24 Verfahren zur proteinrückfaltung durch verwendung zwitterionischer wirkstoffe von niedrigem molekulargewicht

Country Status (8)

Country Link
US (5) US20020052026A1 (de)
EP (1) EP1021528B1 (de)
JP (1) JP2001519149A (de)
AT (1) ATE258598T1 (de)
AU (1) AU757692B2 (de)
CA (1) CA2305559A1 (de)
DE (1) DE69821376D1 (de)
WO (1) WO1999018196A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19755960C1 (de) * 1997-12-16 1998-11-26 Hoechst Ag Verfahren zum Aufschluß von biologischem Material
FR2801219A1 (fr) * 2000-09-18 2001-05-25 Pf Medicament Procede de preparation de proteine recombinante renaturee en presence de detergent
EP1314739A1 (de) * 2001-11-22 2003-05-28 Bayer Ag Verfahren zur Renaturierung rekombinanter, Disulfid-enthaltender Proteine bei hohen Proteinkonzentrationen in Gegenwart von Aminen
US8143210B2 (en) 2002-02-14 2012-03-27 The Board Of Trustees Of The Leland Stanford Junior University Enzyme treatment of foodstuffs for celiac sprue
EP2364718B1 (de) 2002-02-14 2016-07-06 The Board of Trustees of the Leland Stanford Junior University Enzymbehandlung von Nahrungsmitteln für Zöliakie
JP4401618B2 (ja) 2002-04-15 2010-01-20 キヤノン株式会社 記録装置、及び、バッファ管理方法
SI21273A (sl) * 2002-07-31 2004-02-29 LEK farmacevtska dru�ba d.d. Priprava inkluzijskih teles z visokim deležem pravilno zvitega prekurzorja heterolognega proteina
EP1563300B1 (de) 2002-11-20 2012-04-18 The Board Of Trustees Of The Leland Stanford Junior University Diagnoseverfahren für sprue/zöliakie
EP1449848A1 (de) * 2003-02-20 2004-08-25 GBF German Research Centre for Biotechnology Verfahren zur Herstellung von Proteinen aus der Cystine-Knoten-Familie
US7579313B2 (en) 2003-11-18 2009-08-25 The Board Of Trustees Of The Leland Stanford Junior University Transglutaminase inhibitors and methods of use thereof
GB0604938D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Proteins, nucleic acids and medicaments
GB0604964D0 (en) * 2006-03-11 2006-04-19 Renovo Ltd Protein folding
GB0604966D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Medicaments and proteins
CA2680590C (en) * 2007-03-16 2017-10-17 Jonathan David Gass Combination enzyme therapy for digestion of dietary gluten
US8148105B2 (en) * 2007-03-16 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Scaleable manufacturing process for cysteine endoprotease B, isoform 2
CA2692585A1 (en) * 2007-07-03 2010-01-04 Amgen Inc. Measurement of protein using inclusion body dry weight
EA020621B1 (ru) 2009-06-22 2014-12-30 Амген Инк. Рефолдинг белков с использованием химически контролируемого окислительно-восстановительного состояния
JP2012531428A (ja) 2009-06-25 2012-12-10 アムジエン・インコーポレーテツド 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス
US20140148387A1 (en) 2010-09-01 2014-05-29 Masahiro Nagaya Freeze-dried recombinant human bone morphogenetic protein-2 preparation
US9630916B2 (en) 2014-08-19 2017-04-25 Wisconsin Alumni Research Foundation Organocatalysts of oxidative protein folding
WO2017040363A1 (en) 2015-09-02 2017-03-09 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds
JOP20210162A1 (ar) * 2018-12-27 2023-01-30 Astellas Pharma Inc جسيم نانوي، وعامل تباين للتصوير بالرنين المغناطيسي يشتمل على الجسيم النانوي، ومركب ترابطي ثنائي القطب
WO2021050009A2 (en) * 2019-09-13 2021-03-18 National Science And Technology Development Agency Composition of renaturation buffer solution for dimeric proteins and method of renaturation dimeric proteins using the composition thereof
CN119684427A (zh) * 2024-12-25 2025-03-25 无锡傲锐东源生物科技有限公司 一种重组bmp4蛋白复性液及重组bmp4蛋白的制备方法

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU597924B2 (en) * 1985-12-11 1990-06-14 Natinco Nv Solubilization of protein aggregates
ZA874681B (en) 1986-07-01 1988-04-27 Genetics Inst Novel osteoinductive factors
US5187076A (en) 1986-07-01 1993-02-16 Genetics Institute, Inc. DNA sequences encoding BMP-6 proteins
US5013649A (en) 1986-07-01 1991-05-07 Genetics Institute, Inc. DNA sequences encoding osteoinductive products
US5106748A (en) 1986-07-01 1992-04-21 Genetics Institute, Inc. Dna sequences encoding 5 proteins
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
ATE209251T1 (de) 1990-05-16 2001-12-15 Genetics Inst Knochen- und knorpel-bildung hervorrufende proteine
KR100255415B1 (ko) 1991-06-25 2000-05-01 브루스 엠. 에이센 비엠피-9 조성물
DE69327645T2 (de) 1992-02-12 2000-09-07 Biopharm Gesellschaft Zur Biotechnologischen Entwicklung Von Pharmaka Mbh Dna-sequenzen kodierend für neuartige wachstums-/differentierungsfaktoren
DE4208568A1 (de) 1992-03-18 1993-09-23 Zimmer Ag Verfahren und vorrichtung zur herstellung synthetischer endlosfilamente
IL106278A0 (en) 1992-07-13 1993-11-15 Sumitomo Metal Ind Bone formation-inducing protein
EP0679163A4 (de) 1993-01-12 1997-07-16 Univ Johns Hopkins Med Wachstums-differentierungsfaktor-3.
EP0690871A4 (de) 1993-01-12 1999-10-20 Univ Johns Hopkins Med Wachstums-differenzierungsfaktor-5
WO1994015966A1 (en) 1993-01-12 1994-07-21 Johns Hopkins University School Of Medicine Growth differentiation factor-9
ES2201076T3 (es) 1993-03-19 2004-03-16 The Johns Hopkins University School Of Medicine Factor-8 de diferenciacion del crecimiento.
JPH09501305A (ja) 1993-05-12 1997-02-10 ジェネティックス・インスティテュート・インコーポレイテッド Ｂｍｐ−１０組成物
EP0698094B1 (de) 1993-05-12 2004-02-04 Genetics Institute, LLC Bmp-11 zusammensetzungen
WO1995001802A1 (en) 1993-07-09 1995-01-19 The Johns Hopkins University School Of Medicine Growth differentiation factor-7
WO1995001801A1 (en) 1993-07-09 1995-01-19 The Johns Hopkins University School Of Medicine Growth differentiation factor-6
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
CA2171546A1 (en) 1993-10-08 1995-04-20 Se-Jin Lee Growth differentiation factor-10
US5399677A (en) * 1993-12-07 1995-03-21 Genetics Institute, Inc. Mutants of bone morphogenetic proteins
ES2255059T3 (es) 1993-12-07 2006-06-16 Genetics Institute, Llc Bmp-12, bmp-13 y composiciones suyas inductoras de tendon.
FR2714906B1 (fr) 1994-01-12 1996-02-02 Commissariat Energie Atomique Agents de solubilisation de biomolécules constitués par des zwitterions non détergents et leurs utilisations.
JPH07250688A (ja) 1994-01-28 1995-10-03 Sagami Chem Res Center ＴＧＦ−βスーパーファミリー蛋白質をコードするヒト新規ｃＤＮＡ
DK0776337T3 (da) 1994-07-08 2005-12-12 Univ Johns Hopkins Med Vækstdifferentieringsfaktor-11
US5929213A (en) 1994-07-13 1999-07-27 The Johns Hopkins University School Of Medicine Antibodies to growth differentiation factor-12
US5635372A (en) 1995-05-18 1997-06-03 Genetics Institute, Inc. BMP-15 compositions

Also Published As

Publication number Publication date
AU9116798A (en) 1999-04-27
US20020052026A1 (en) 2002-05-02
US6593106B1 (en) 2003-07-15
AU757692B2 (en) 2003-03-06
WO1999018196A1 (en) 1999-04-15
US6593107B1 (en) 2003-07-15
EP1021528B1 (de) 2004-01-28
US6632635B1 (en) 2003-10-14
EP1021528A1 (de) 2000-07-26
JP2001519149A (ja) 2001-10-23
US6596511B1 (en) 2003-07-22
DE69821376D1 (de) 2004-03-04
CA2305559A1 (en) 1999-04-15

Similar Documents

Publication Publication Date Title
ATE258598T1 (de) Verfahren zur proteinrückfaltung durch verwendung zwitterionischer wirkstoffe von niedrigem molekulargewicht
ATE449186T1 (de) Zusammensetzungen und verfahren zur selektiven nukleinsäureisolierung
BR9915679A (pt) Composições e métodos para aumentar a mineralização óssea
DE69825521D1 (de) Verfahren zur Herstellung von Silicon-in-Wasser-Emulsionen
DE50303749D1 (de) Verfahren zur herstellung von phthalocyaninpigmentzubereitungen
DE69835366D1 (de) Verfahren zur herstellung von perfluorvinylethersulfonsäure-derivaten
BR9509665A (pt) Composto composições e métodos
DE59809115D1 (de) Verfahren zur Herstellung von Polysiloxan-Polycarbonat-Blockcokondensaten
DE50112802D1 (de) Peptide, deren herstellung und verwendung zur bindung von immunglobulinen
EA199900643A1 (ru) Сульфамидные ингибиторы металлопротеаз
ATE263836T1 (de) Chimere proteine, welche borrelia polypeptide beinhalten, verwendungen dafür
ATE220558T1 (de) Verbessertes verfahren zur stabilisierung von proteinen
DE69822251D1 (de) Verfahren zur Herstellung von mikrobieller Transglutaminase
ATE207205T1 (de) Verfahren zumm assay von endotoxin-konzentration
DE602004032345D1 (de) Reagenzien und verfahren zur bildung von disulfid-bindungen und glykosylierung von proteinen
DE69909208D1 (de) Verfahren zur Herstellung von Alkylhalogensilanen
DE69918605D1 (de) Verfahren zur Herstellung von Kaliumoxonat
EA200000522A1 (ru) 5-htагонисты
SE9602822D0 (sv) New receptor
ATE280774T1 (de) Verfahren und zusammensetzungen zur raschen aufreinigung von proteasomen und verfahren zur verwendung entsprechender komponenten
AR022150A1 (es) Acidos nucleicos derivados de monocitos y composiciones y metodos relacionados
DE59807115D1 (de) Verfahren zur Herstellung von Alkoxysilanen
DE59811148D1 (de) Verfahren zur Herstellung von Methylendi(phenylamin)
WO2002012541A3 (en) Novel motor proteins and methods for their use
DE59802620D1 (de) Verfahren zur Herstellung von trans-(R,R)-Actinol

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties