ATE236183T1 - Verfahren zur reinigung von nukleinsäuren - Google Patents

Verfahren zur reinigung von nukleinsäuren

Info

Publication number
ATE236183T1
ATE236183T1 AT97938081T AT97938081T ATE236183T1 AT E236183 T1 ATE236183 T1 AT E236183T1 AT 97938081 T AT97938081 T AT 97938081T AT 97938081 T AT97938081 T AT 97938081T AT E236183 T1 ATE236183 T1 AT E236183T1
Authority
AT
Austria
Prior art keywords
nucleic acids
purifying nucleic
producing
avoids
efficient
Prior art date
Application number
AT97938081T
Other languages
English (en)
Inventor
Lee B Bussey
Robert Adamson
Alan Atchley
Original Assignee
Megabios Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24775118&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE236183(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Megabios Corp filed Critical Megabios Corp
Application granted granted Critical
Publication of ATE236183T1 publication Critical patent/ATE236183T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT97938081T 1996-08-01 1997-07-31 Verfahren zur reinigung von nukleinsäuren ATE236183T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/691,090 US6011148A (en) 1996-08-01 1996-08-01 Methods for purifying nucleic acids
PCT/US1997/013493 WO1998005673A1 (en) 1996-08-01 1997-07-31 Methods for purifying nucleic acids

Publications (1)

Publication Number Publication Date
ATE236183T1 true ATE236183T1 (de) 2003-04-15

Family

ID=24775118

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97938081T ATE236183T1 (de) 1996-08-01 1997-07-31 Verfahren zur reinigung von nukleinsäuren

Country Status (9)

Country Link
US (1) US6011148A (de)
EP (1) EP0923592B1 (de)
JP (1) JP3492702B2 (de)
AT (1) ATE236183T1 (de)
AU (1) AU717136B2 (de)
CA (1) CA2261150C (de)
DE (1) DE69720473T2 (de)
DK (1) DK0923592T3 (de)
WO (1) WO1998005673A1 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2182388C (en) * 1994-02-07 2007-08-07 Metin Colpan Process for producing endotoxin-free or endotoxin-poor nucleic acids and/or oligonucleotides for gene therapy
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
CA2378944A1 (en) 1999-07-23 2001-02-01 Genentech, Inc. Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration
WO2001079486A2 (en) * 2000-04-13 2001-10-25 Millipore Corporation Method and apparatus for plasmid recovery involving ultrafiltration
WO2001085928A1 (en) * 2000-05-10 2001-11-15 Mitsubishi Pharma Corporation Method of preparing virus vector
AU2001265394A1 (en) * 2000-06-07 2001-12-17 Amersham Pharmacia Biotech Inc. Method and apparatus for purifying nucleic acids
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
EP1359228B1 (de) * 2002-04-23 2013-11-27 Accenture Global Services Limited Nukleinsäure authentifikation mittels detektion des streulichtes
WO2004024283A2 (en) * 2002-09-13 2004-03-25 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
JP4723254B2 (ja) * 2002-12-23 2011-07-13 バイカル インコーポレイテッド プラスミドｄｎａを精製するための方法
DK2364769T3 (en) 2003-05-30 2016-04-11 Vgxi Inc Apparatus and methods for biomaterialeproduktion
JP2005095003A (ja) * 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
AU2004282342B2 (en) * 2003-10-09 2009-05-14 Epigenomics Ag Improved bisulfite conversion of DNA
US8298758B2 (en) * 2003-12-26 2012-10-30 Prima Meat Packers, Ltd. Method of multiplex microorganism detection
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
AU2005280356B2 (en) 2004-08-16 2008-09-25 Clague P. Hodgson Improved strains of E. coli for plasmid DNA production
US20060079150A1 (en) * 2004-10-08 2006-04-13 Miva Filoseta Toy for collecting and dispersing toy vehicles
AU2005314431B2 (en) * 2004-12-11 2011-02-17 Cytogenix, Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
AU2007253683B2 (en) * 2006-05-19 2012-12-06 Boston Scientific Scimed, Inc. Method and articles for treatment of stress urinary incontinence
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
JP5714291B2 (ja) * 2010-10-15 2015-05-07 独立行政法人農業・食品産業技術総合研究機構 抗酸菌ｄｎａの抽出精製法
EP2691101A2 (de) 2011-03-31 2014-02-05 Moderna Therapeutics, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
EA201591229A1 (ru) 2013-03-14 2016-01-29 Шир Хьюман Дженетик Терапис, Инк. Способы очистки матричной рнк
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
EP3521429B1 (de) 2013-03-15 2022-05-04 GlaxoSmithKline Biologicals SA Rna-aufreinigungsverfahren
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
EP2971033B8 (de) 2013-03-15 2019-07-10 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
DE102014207774B4 (de) * 2014-04-25 2015-12-31 Robert Bosch Gmbh Verfahren und Vorrichtung zur Aufreinigung von biologischen Molekülen
KR20250005450A (ko) * 2014-04-25 2025-01-09 샤이어 휴먼 지네틱 테라피즈 인크. 메신저 rna 의 정제 방법
EP3294885B1 (de) 2015-05-08 2020-07-01 CureVac Real Estate GmbH Verfahren zur herstellung von rna
DK4108769T3 (da) 2015-05-29 2023-10-09 Curevac Mfg Gmbh Fremgangsmåde til fremstilling og oprensning af rna, omfattende mindst et trin til tangential-flow-filtrering
CA2998370A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a stabilizing tail region
GB201600287D0 (en) * 2016-01-07 2016-02-24 Fujifilm Diosynth Biotechnologies Uk Ltd Process
GB201600290D0 (en) 2016-01-07 2016-02-24 Fujifilm Diosynth Biotechnologies Uk Ltd Process
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
EP3841208A1 (de) 2018-08-24 2021-06-30 Translate Bio, Inc. Verfahren zur reinigung von messenger-rna
EP4329923A1 (de) * 2021-04-30 2024-03-06 Hydranautics Konzentration und diafiltration von oligonukleotiden

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3308932A1 (de) * 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
GB8600582D0 (en) * 1986-01-10 1986-02-19 Ca Minister Nat Defence Purifying biological materials
GB8613476D0 (en) * 1986-06-04 1986-07-09 London Hospital Med Coll Method of sequencing nucleic acids
JP2978518B2 (ja) * 1989-12-06 1999-11-15 東ソー株式会社 ファージｄｎａの精製方法
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA

Also Published As

Publication number Publication date
WO1998005673A1 (en) 1998-02-12
EP0923592A1 (de) 1999-06-23
AU4049097A (en) 1998-02-25
WO1998005673A8 (en) 1999-12-09
JP2000500028A (ja) 2000-01-11
US6011148A (en) 2000-01-04
DE69720473D1 (de) 2003-05-08
CA2261150A1 (en) 1998-02-12
AU717136B2 (en) 2000-03-16
EP0923592B1 (de) 2003-04-02
DE69720473T2 (de) 2004-05-06
DK0923592T3 (da) 2003-07-21
JP3492702B2 (ja) 2004-02-03
CA2261150C (en) 2004-04-13

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