ATE236183T1 - Verfahren zur reinigung von nukleinsäuren - Google Patents

Verfahren zur reinigung von nukleinsäuren

Info

Publication number
ATE236183T1
ATE236183T1 AT97938081T AT97938081T ATE236183T1 AT E236183 T1 ATE236183 T1 AT E236183T1 AT 97938081 T AT97938081 T AT 97938081T AT 97938081 T AT97938081 T AT 97938081T AT E236183 T1 ATE236183 T1 AT E236183T1
Authority
AT
Austria
Prior art keywords
nucleic acids
purifying nucleic
producing
avoids
efficient
Prior art date
Application number
AT97938081T
Other languages
English (en)
Inventor
Lee B Bussey
Robert Adamson
Alan Atchley
Original Assignee
Megabios Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24775118&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE236183(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Megabios Corp filed Critical Megabios Corp
Application granted granted Critical
Publication of ATE236183T1 publication Critical patent/ATE236183T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT97938081T 1996-08-01 1997-07-31 Verfahren zur reinigung von nukleinsäuren ATE236183T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/691,090 US6011148A (en) 1996-08-01 1996-08-01 Methods for purifying nucleic acids
PCT/US1997/013493 WO1998005673A1 (en) 1996-08-01 1997-07-31 Methods for purifying nucleic acids

Publications (1)

Publication Number Publication Date
ATE236183T1 true ATE236183T1 (de) 2003-04-15

Family

ID=24775118

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97938081T ATE236183T1 (de) 1996-08-01 1997-07-31 Verfahren zur reinigung von nukleinsäuren

Country Status (9)

Country Link
US (1) US6011148A (de)
EP (1) EP0923592B1 (de)
JP (1) JP3492702B2 (de)
AT (1) ATE236183T1 (de)
AU (1) AU717136B2 (de)
CA (1) CA2261150C (de)
DE (1) DE69720473T2 (de)
DK (1) DK0923592T3 (de)
WO (1) WO1998005673A1 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0775150B1 (de) * 1994-02-07 1999-04-28 QIAGEN GmbH Verfahren zur abreicherung oder entfernung von endotoxinen
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
JP2003505077A (ja) * 1999-07-23 2003-02-12 ジェネンテック・インコーポレーテッド 平行流ろ過法を用いるｒｎａｓｅ−及び有機溶媒−非含有プラスミドｄｎａの精製方法
DE1272627T1 (de) 2000-04-13 2003-05-28 Millipore Corp., Bedford Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration
WO2001085928A1 (en) * 2000-05-10 2001-11-15 Mitsubishi Pharma Corporation Method of preparing virus vector
WO2001094574A2 (en) * 2000-06-07 2001-12-13 Amersham Biosciences Corp Method and apparatus for purifying nucleic acids
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
EP1359228B1 (de) * 2002-04-23 2013-11-27 Accenture Global Services Limited Nukleinsäure authentifikation mittels detektion des streulichtes
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
WO2004060277A2 (en) * 2002-12-23 2004-07-22 Vical Incorporated Process for purification of plasmid dna
US7238522B2 (en) * 2003-05-30 2007-07-03 Advisys, Inc. Devices and methods for biomaterial production
JP2005095003A (ja) * 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
EP2088210A3 (de) * 2003-10-09 2009-11-18 Epigenomics AG Verbesserte Bisulfit-Umwandlung von DNA
US8298758B2 (en) * 2003-12-26 2012-10-30 Prima Meat Packers, Ltd. Method of multiplex microorganism detection
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
BRPI0515418A (pt) 2004-08-16 2008-07-22 Nature Technology Corp método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria
US20060079150A1 (en) * 2004-10-08 2006-04-13 Miva Filoseta Toy for collecting and dispersing toy vehicles
WO2006063355A2 (en) * 2004-12-11 2006-06-15 Cytogenix , Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
AU2007253683B2 (en) * 2006-05-19 2012-12-06 Boston Scientific Scimed, Inc. Method and articles for treatment of stress urinary incontinence
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
JP5714291B2 (ja) * 2010-10-15 2015-05-07 独立行政法人農業・食品産業技術総合研究機構 抗酸菌ｄｎａの抽出精製法
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
EA201591229A1 (ru) 2013-03-14 2016-01-29 Шир Хьюман Дженетик Терапис, Инк. Способы очистки матричной рнк
EP2971033B8 (de) 2013-03-15 2019-07-10 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
EP4279610A3 (de) 2013-03-15 2024-01-03 ModernaTX, Inc. Ribonukleinsäurereinigung
PL2970948T3 (pl) 2013-03-15 2019-06-28 Glaxosmithkline Biologicals Sa Sposoby oczyszczania rna
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
DE102014207774B4 (de) * 2014-04-25 2015-12-31 Robert Bosch Gmbh Verfahren und Vorrichtung zur Aufreinigung von biologischen Molekülen
EP4729528A3 (de) * 2014-04-25 2026-06-24 Translate Bio, Inc. Verfahren zur reinigung von messenger-rna
WO2016180430A1 (en) 2015-05-08 2016-11-17 Curevac Ag Method for producing rna
DK3303583T3 (da) * 2015-05-29 2020-07-13 Curevac Real Estate Gmbh Fremgangsmåde til fremstilling og oprensning af rna, omfattende mindst et trin til tangential-flow-filtrering
EP4101930A1 (de) 2015-09-17 2022-12-14 ModernaTX, Inc. Polynukleotide mit stabilisierendem schwanzbereich
GB201600290D0 (en) 2016-01-07 2016-02-24 Fujifilm Diosynth Biotechnologies Uk Ltd Process
GB201600287D0 (en) * 2016-01-07 2016-02-24 Fujifilm Diosynth Biotechnologies Uk Ltd Process
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
US11174500B2 (en) 2018-08-24 2021-11-16 Translate Bio, Inc. Methods for purification of messenger RNA
EP4329923A1 (de) * 2021-04-30 2024-03-06 Hydranautics Konzentration und diafiltration von oligonukleotiden

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3308932A1 (de) * 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
GB8600582D0 (en) * 1986-01-10 1986-02-19 Ca Minister Nat Defence Purifying biological materials
GB8613476D0 (en) * 1986-06-04 1986-07-09 London Hospital Med Coll Method of sequencing nucleic acids
JP2978518B2 (ja) * 1989-12-06 1999-11-15 東ソー株式会社 ファージｄｎａの精製方法
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA

Also Published As

Publication number Publication date
AU717136B2 (en) 2000-03-16
US6011148A (en) 2000-01-04
JP3492702B2 (ja) 2004-02-03
JP2000500028A (ja) 2000-01-11
WO1998005673A1 (en) 1998-02-12
WO1998005673A8 (en) 1999-12-09
CA2261150C (en) 2004-04-13
CA2261150A1 (en) 1998-02-12
AU4049097A (en) 1998-02-25
EP0923592A1 (de) 1999-06-23
DK0923592T3 (da) 2003-07-21
DE69720473D1 (de) 2003-05-08
DE69720473T2 (de) 2004-05-06
EP0923592B1 (de) 2003-04-02

Similar Documents

Publication Publication Date Title
DE69720473D1 (de) Verfahren zur reinigung von nukleinsäuren
ATE239090T1 (de) Methode zum generieren von einzelsträngigen dna molekülen
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
ATE278785T1 (de) Neues verfahren zur gewinnung und reinigung von fusionsproteinen
YU29796A (sh) Postupak za prečišćavanje plazmida u velikoj razmeri
DE69627698T2 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
BR9707948A (pt) Processo para obter plantas transplastômicas
NO985427D0 (no) Modulatorer for vevsregenerering
ATE356222T1 (de) Massives parallelverfahren zur dekodierung von dna und rna
ATE226960T1 (de) Verfahren zur herstellung von cardiodilatin- fragmenten, hochgereinigte cardiodilatin- fragmente und zwischenprodukte zu deren herstellung
EP1187840A4 (de) Methoden zur reinigung von dns und gereinigte dns
ATE235547T1 (de) Verfahren zur reduzierung der rns-konzentration in einer mischung biologischen materials unter verwendung von diatomeen-erde
DE50012831D1 (de) Verfahren zur verbindung von molekularen substanzen
DE69502313D1 (de) Verfahren zur biologischen Reinigung von Gasströmen
EP1578763A4 (de) Reinigungsverfahren für plasmid-dna
ATE212382T1 (de) Verfahren zur sequenzierung durch oligomerhybridisierung
EP0981535A4 (de) Verfahren zur herstellung und reinigung von nukleinsäuremolekülen
CA2316113A1 (en) Method for nucleic acid purification using iodine
WO1996039501A3 (en) Oligonucleotides specific for human papillomavirus
DE69024392D1 (de) Verfahren zur Reinigung von Aussenmembran-Protein von Haemophilus influenza
DE69331216D1 (de) Planze resistent gegen mindestens zwei viren und dessen preparation
ATE278699T1 (de) Verfahren zur aufreinigung von nukleinsäuren sowie anionenaustauscher zur durchführung dieses verfahrens
BR9709700A (pt) Vetor viral célula composição farmacêtica processo de preparação in situ de moléculas de dna circulares replicativas utilização de um vetor viral processo paa a transferência de ácidos nucleícos para uma e molécula de dna circular replicativa
ATE224907T1 (de) Verfahren zur reinigung von roxithromycin
ES2196157T3 (es) Oligonucleotidos especificos para el virus de la hepatitis c.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties