ATE236183T1 - Verfahren zur reinigung von nukleinsäuren - Google Patents

Verfahren zur reinigung von nukleinsäuren

Info

Publication number
ATE236183T1
ATE236183T1 AT97938081T AT97938081T ATE236183T1 AT E236183 T1 ATE236183 T1 AT E236183T1 AT 97938081 T AT97938081 T AT 97938081T AT 97938081 T AT97938081 T AT 97938081T AT E236183 T1 ATE236183 T1 AT E236183T1
Authority
AT
Austria
Prior art keywords
nucleic acids
purifying nucleic
producing
avoids
efficient
Prior art date
Application number
AT97938081T
Other languages
English (en)
Inventor
Lee B Bussey
Robert Adamson
Alan Atchley
Original Assignee
Megabios Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24775118&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE236183(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Megabios Corp filed Critical Megabios Corp
Application granted granted Critical
Publication of ATE236183T1 publication Critical patent/ATE236183T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT97938081T 1996-08-01 1997-07-31 Verfahren zur reinigung von nukleinsäuren ATE236183T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/691,090 US6011148A (en) 1996-08-01 1996-08-01 Methods for purifying nucleic acids
PCT/US1997/013493 WO1998005673A1 (en) 1996-08-01 1997-07-31 Methods for purifying nucleic acids

Publications (1)

Publication Number Publication Date
ATE236183T1 true ATE236183T1 (de) 2003-04-15

Family

ID=24775118

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97938081T ATE236183T1 (de) 1996-08-01 1997-07-31 Verfahren zur reinigung von nukleinsäuren

Country Status (9)

Country Link
US (1) US6011148A (de)
EP (1) EP0923592B1 (de)
JP (1) JP3492702B2 (de)
AT (1) ATE236183T1 (de)
AU (1) AU717136B2 (de)
CA (1) CA2261150C (de)
DE (1) DE69720473T2 (de)
DK (1) DK0923592T3 (de)
WO (1) WO1998005673A1 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE59507433D1 (de) * 1994-02-07 2000-01-20 Qiagen Gmbh Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
CN1378592A (zh) * 1999-07-23 2002-11-06 杰南技术公司 在无rna酶和有机溶剂的条件下应用切向流过滤来纯化质粒dna的方法
ES2184661T3 (es) * 2000-04-13 2005-09-01 Millipore Corporation Metodo y aparato para la recuperacion de plasmidos que implica ultrafiltracion.
WO2001085928A1 (fr) * 2000-05-10 2001-11-15 Mitsubishi Pharma Corporation Procede de preparation de vecteur de virus
AU2001265394A1 (en) * 2000-06-07 2001-12-17 Amersham Pharmacia Biotech Inc. Method and apparatus for purifying nucleic acids
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
EP1359228B1 (de) * 2002-04-23 2013-11-27 Accenture Global Services Limited Nukleinsäure authentifikation mittels detektion des streulichtes
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
EP1578763B1 (de) * 2002-12-23 2009-01-07 Vical Incorporated Reinigungsverfahren für plasmid-dna
CA2567324C (en) 2003-05-30 2012-01-03 Advisys, Inc. Devices and methods for biomaterial production
JP2005095003A (ja) * 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
JP4571643B2 (ja) * 2003-10-09 2010-10-27 エピゲノミクス アーゲー 改良された重亜硫酸塩によるｄｎａの変換
US8298758B2 (en) * 2003-12-26 2012-10-30 Prima Meat Packers, Ltd. Method of multiplex microorganism detection
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
EA010864B1 (ru) 2004-08-16 2008-12-30 Нейче Текнолоджи Корп. Улучшенные штаммы e.coli для продуцирования плазмидной днк
US20060079150A1 (en) * 2004-10-08 2006-04-13 Miva Filoseta Toy for collecting and dispersing toy vehicles
BRPI0515777A (pt) * 2004-12-11 2008-08-05 Cytogenix Inc biossìntese isenta de células de ácido nucléico de alta qualidade e usos dos mesmos
AU2007253683B2 (en) * 2006-05-19 2012-12-06 Boston Scientific Scimed, Inc. Method and articles for treatment of stress urinary incontinence
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
JP5714291B2 (ja) * 2010-10-15 2015-05-07 独立行政法人農業・食品産業技術総合研究機構 抗酸菌ｄｎａの抽出精製法
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
EA201591229A1 (ru) * 2013-03-14 2016-01-29 Шир Хьюман Дженетик Терапис, Инк. Способы очистки матричной рнк
WO2014152031A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Ribonucleic acid purification
US11155572B2 (en) 2013-03-15 2021-10-26 Glaxosmithkline Biologicals Sa RNA purification methods
WO2014152027A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Manufacturing methods for production of rna transcripts
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
DE102014207774B4 (de) * 2014-04-25 2015-12-31 Robert Bosch Gmbh Verfahren und Vorrichtung zur Aufreinigung von biologischen Molekülen
WO2015164773A1 (en) * 2014-04-25 2015-10-29 Shire Human Genetic Therapies, Inc. Methods for purification of messenger rna
DK3294885T3 (da) 2015-05-08 2020-08-10 Curevac Real Estate Gmbh Fremgangsmåde til at fremstille rna
WO2016193206A1 (en) 2015-05-29 2016-12-08 Curevac Ag A method for producing and purifying rna, comprising at least one step of tangential flow filtration
GB201600287D0 (en) * 2016-01-07 2016-02-24 Fujifilm Diosynth Biotechnologies Uk Ltd Process
GB201600290D0 (en) 2016-01-07 2016-02-24 Fujifilm Diosynth Biotechnologies Uk Ltd Process
US20200318098A1 (en) * 2016-06-24 2020-10-08 Modernatx, Inc. Methods and apparatus for filtration
WO2020041793A1 (en) 2018-08-24 2020-02-27 Translate Bio, Inc. Methods for purification of messenger rna
EP4329923A1 (de) * 2021-04-30 2024-03-06 Hydranautics Konzentration und diafiltration von oligonukleotiden

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3308932A1 (de) * 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
GB8600582D0 (en) * 1986-01-10 1986-02-19 Ca Minister Nat Defence Purifying biological materials
GB8613476D0 (en) * 1986-06-04 1986-07-09 London Hospital Med Coll Method of sequencing nucleic acids
JP2978518B2 (ja) * 1989-12-06 1999-11-15 東ソー株式会社 ファージｄｎａの精製方法
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA

Also Published As

Publication number Publication date
US6011148A (en) 2000-01-04
DK0923592T3 (da) 2003-07-21
CA2261150C (en) 2004-04-13
EP0923592A1 (de) 1999-06-23
WO1998005673A1 (en) 1998-02-12
JP2000500028A (ja) 2000-01-11
AU4049097A (en) 1998-02-25
CA2261150A1 (en) 1998-02-12
DE69720473D1 (de) 2003-05-08
AU717136B2 (en) 2000-03-16
EP0923592B1 (de) 2003-04-02
DE69720473T2 (de) 2004-05-06
WO1998005673A8 (en) 1999-12-09
JP3492702B2 (ja) 2004-02-03

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