EP0981535A4 - Verfahren zur herstellung und reinigung von nukleinsäuremolekülen - Google Patents

Verfahren zur herstellung und reinigung von nukleinsäuremolekülen

Info

Publication number
EP0981535A4
EP0981535A4 EP98922202A EP98922202A EP0981535A4 EP 0981535 A4 EP0981535 A4 EP 0981535A4 EP 98922202 A EP98922202 A EP 98922202A EP 98922202 A EP98922202 A EP 98922202A EP 0981535 A4 EP0981535 A4 EP 0981535A4
Authority
EP
European Patent Office
Prior art keywords
purification
production
methods
nucleic acid
acid molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98922202A
Other languages
English (en)
French (fr)
Other versions
EP0981535A1 (de
Inventor
Christian E Gruber
Joel A Jessee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Inc
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Publication of EP0981535A1 publication Critical patent/EP0981535A1/de
Publication of EP0981535A4 publication Critical patent/EP0981535A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
EP98922202A 1997-05-12 1998-05-12 Verfahren zur herstellung und reinigung von nukleinsäuremolekülen Withdrawn EP0981535A4 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US4621997P 1997-05-12 1997-05-12
US46219P 1997-05-12
PCT/US1998/009586 WO1998051699A1 (en) 1997-05-12 1998-05-12 Methods for production and purification of nucleic acid molecules
CA002287525A CA2287525A1 (en) 1997-05-12 1999-11-12 Methods for production and purification of nucleic acid molecules

Publications (2)

Publication Number Publication Date
EP0981535A1 EP0981535A1 (de) 2000-03-01
EP0981535A4 true EP0981535A4 (de) 2000-11-29

Family

ID=25681276

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98922202A Withdrawn EP0981535A4 (de) 1997-05-12 1998-05-12 Verfahren zur herstellung und reinigung von nukleinsäuremolekülen

Country Status (4)

Country Link
EP (1) EP0981535A4 (de)
JP (1) JP2002502237A (de)
CA (1) CA2287525A1 (de)
WO (1) WO1998051699A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122304B2 (en) * 1997-05-12 2006-10-17 Whatman, Inc. Methods for the storage and synthesis of nucleic acids using a solid support
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
WO2001066802A1 (en) * 2000-03-09 2001-09-13 Genetag Technology, Inc. SYSTEMS AND METHODS TO QUANTIFY AND AMPLIFY BOTH SIGNALING AND PROBES FOR cDNA CHIPS AND GENES EXPRESSION MICROARRAYS
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
JP2004511211A (ja) 2000-05-26 2004-04-15 インヴィトロジェン　コーポレーション 耐熱性逆転写酵素およびその使用
DE10120798B4 (de) * 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
EP2325303B1 (de) 2002-09-13 2013-11-06 Life Technologies Corporation Wärmestabile reverse Transkriptasen und Verwendungen davon
US20060064247A1 (en) * 2004-07-14 2006-03-23 Shao-Min Yuan Methods and systems for in silico experimental design and for providing a biotechnology product to a customer
EP2581447A1 (de) * 2011-10-12 2013-04-17 Royal College of Surgeons in Ireland Selektive Isolierung eines Boten-RNA-Moleküls mit seinen daran gebundenen artverwandten Mikro-RNA-Molekülen
JP2014176303A (ja) 2013-03-13 2014-09-25 Seiko Epson Corp ｃＤＮＡの合成方法

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012063A1 (en) * 1988-06-01 1989-12-14 The United States Of America, As Represented By Th Sequencing dna; a modification of the polymerase chain reaction
WO1990006044A2 (en) * 1988-11-21 1990-06-14 Dynal As PROCESS FOR PRODUCING cDNA
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
WO1991018114A1 (en) * 1990-05-22 1991-11-28 Medical Research Council Polynucleotide amplification
EP0622457A1 (de) * 1992-07-21 1994-11-02 Sumitomo Electric Industries, Ltd. Methoden zur trennung zellspezifischer cdna und herstellung von entsprechenden genbanken
EP0645449A1 (de) * 1993-09-24 1995-03-29 Roche Diagnostics GmbH Verfahren zur spezifischen Klonierung von Nukleinsäuren
EP0735144A1 (de) * 1995-03-28 1996-10-02 Research Development Corporation Of Japan Verfahren zur Molekular-Indexierung (Kategorisieren) von exprimierten Genen unter Verwendung von Restriktionsenzymen
EP0761822A2 (de) * 1995-09-12 1997-03-12 The Johns Hopkins University School Of Medicine Reihenanalyse-Verfahren der Genexpression
WO1998014619A1 (en) * 1996-10-03 1998-04-09 Incyte Pharmaceuticals, Inc. Methods for generating and analyzing transcript markers
EP0872559A1 (de) * 1995-11-09 1998-10-21 Shionogi & Co., Ltd. Verfahren zur gleichzeitigen erkennung von polymeren

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5726022A (en) * 1996-11-18 1998-03-10 Lifespan Biosciences, Inc. Subtractive hybridization and capture methods and kits for differential isolation of nucleic acids including disease-associated sequences

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012063A1 (en) * 1988-06-01 1989-12-14 The United States Of America, As Represented By Th Sequencing dna; a modification of the polymerase chain reaction
WO1990006044A2 (en) * 1988-11-21 1990-06-14 Dynal As PROCESS FOR PRODUCING cDNA
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
WO1991018114A1 (en) * 1990-05-22 1991-11-28 Medical Research Council Polynucleotide amplification
EP0622457A1 (de) * 1992-07-21 1994-11-02 Sumitomo Electric Industries, Ltd. Methoden zur trennung zellspezifischer cdna und herstellung von entsprechenden genbanken
EP0645449A1 (de) * 1993-09-24 1995-03-29 Roche Diagnostics GmbH Verfahren zur spezifischen Klonierung von Nukleinsäuren
EP0735144A1 (de) * 1995-03-28 1996-10-02 Research Development Corporation Of Japan Verfahren zur Molekular-Indexierung (Kategorisieren) von exprimierten Genen unter Verwendung von Restriktionsenzymen
EP0761822A2 (de) * 1995-09-12 1997-03-12 The Johns Hopkins University School Of Medicine Reihenanalyse-Verfahren der Genexpression
EP0872559A1 (de) * 1995-11-09 1998-10-21 Shionogi & Co., Ltd. Verfahren zur gleichzeitigen erkennung von polymeren
WO1998014619A1 (en) * 1996-10-03 1998-04-09 Incyte Pharmaceuticals, Inc. Methods for generating and analyzing transcript markers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO9851699A1 *

Also Published As

Publication number Publication date
JP2002502237A (ja) 2002-01-22
EP0981535A1 (de) 2000-03-01
WO1998051699A1 (en) 1998-11-19
CA2287525A1 (en) 2001-05-12

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