CA2287525A1 - Methods for production and purification of nucleic acid molecules - Google Patents

Methods for production and purification of nucleic acid molecules Download PDF

Info

Publication number
CA2287525A1
CA2287525A1 CA002287525A CA2287525A CA2287525A1 CA 2287525 A1 CA2287525 A1 CA 2287525A1 CA 002287525 A CA002287525 A CA 002287525A CA 2287525 A CA2287525 A CA 2287525A CA 2287525 A1 CA2287525 A1 CA 2287525A1
Authority
CA
Canada
Prior art keywords
nucleic acid
molecule
primer
beta
alpha
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002287525A
Other languages
English (en)
French (fr)
Inventor
Christian E. Gruber
Joel A. Jessee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to PCT/US1998/009586 priority Critical patent/WO1998051699A1/en
Priority to JP54938098A priority patent/JP2002502237A/ja
Priority to EP98922202A priority patent/EP0981535A4/de
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Priority to CA002287525A priority patent/CA2287525A1/en
Priority to US09/725,897 priority patent/US7122304B2/en
Publication of CA2287525A1 publication Critical patent/CA2287525A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002287525A 1997-05-12 1999-11-12 Methods for production and purification of nucleic acid molecules Abandoned CA2287525A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
PCT/US1998/009586 WO1998051699A1 (en) 1997-05-12 1998-05-12 Methods for production and purification of nucleic acid molecules
JP54938098A JP2002502237A (ja) 1997-05-12 1998-05-12 核酸分子の生成および精製のための方法
EP98922202A EP0981535A4 (de) 1997-05-12 1998-05-12 Verfahren zur herstellung und reinigung von nukleinsäuremolekülen
CA002287525A CA2287525A1 (en) 1997-05-12 1999-11-12 Methods for production and purification of nucleic acid molecules
US09/725,897 US7122304B2 (en) 1997-05-12 2000-11-30 Methods for the storage and synthesis of nucleic acids using a solid support

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4621997P 1997-05-12 1997-05-12
CA002287525A CA2287525A1 (en) 1997-05-12 1999-11-12 Methods for production and purification of nucleic acid molecules

Publications (1)

Publication Number Publication Date
CA2287525A1 true CA2287525A1 (en) 2001-05-12

Family

ID=25681276

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002287525A Abandoned CA2287525A1 (en) 1997-05-12 1999-11-12 Methods for production and purification of nucleic acid molecules

Country Status (4)

Country Link
EP (1) EP0981535A4 (de)
JP (1) JP2002502237A (de)
CA (1) CA2287525A1 (de)
WO (1) WO1998051699A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122304B2 (en) * 1997-05-12 2006-10-17 Whatman, Inc. Methods for the storage and synthesis of nucleic acids using a solid support
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
WO2001066802A1 (en) * 2000-03-09 2001-09-13 Genetag Technology, Inc. SYSTEMS AND METHODS TO QUANTIFY AND AMPLIFY BOTH SIGNALING AND PROBES FOR cDNA CHIPS AND GENES EXPRESSION MICROARRAYS
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
US20040209276A1 (en) 2002-09-13 2004-10-21 Smith Michael D. Thermostable reverse transcriptases and uses thereof
NZ522500A (en) 2000-05-26 2004-10-29 Invitrogen Corp Thermostable reverse transcriptases and uses thereof
DE10120798B4 (de) * 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
WO2006019892A2 (en) * 2004-07-14 2006-02-23 Invitrogen Corporation Methods and systems for in silico experimental design and for providing a biotechnology product to a customer
EP2581447A1 (de) * 2011-10-12 2013-04-17 Royal College of Surgeons in Ireland Selektive Isolierung eines Boten-RNA-Moleküls mit seinen daran gebundenen artverwandten Mikro-RNA-Molekülen
JP2014176303A (ja) 2013-03-13 2014-09-25 Seiko Epson Corp ｃＤＮＡの合成方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
CA2003500C (en) * 1988-11-21 2000-01-18 Erik Hornes Process for producing cdna
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
GB9011454D0 (en) * 1990-05-22 1990-07-11 Medical Res Council Polynucleotide amplification
JP3315439B2 (ja) * 1992-07-21 2002-08-19 住友電気工業株式会社 細胞特異的ｃＤＮＡの分離法及びライブラリー作製法
DE4332463A1 (de) * 1993-09-24 1995-03-30 Boehringer Mannheim Gmbh Verfahren zur spezifischen Klonierung von Nukleinsäuren
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5707807A (en) * 1995-03-28 1998-01-13 Research Development Corporation Of Japan Molecular indexing for expressed gene analysis
US5866330A (en) * 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
WO1997017466A1 (fr) * 1995-11-09 1997-05-15 Shionogi & Co., Ltd. Procede de detection simultanee de polygenes
AU4753397A (en) * 1996-10-03 1998-04-24 Incyte Pharmaceuticals, Inc. Methods for generating and analyzing transcript markers
US5726022A (en) * 1996-11-18 1998-03-10 Lifespan Biosciences, Inc. Subtractive hybridization and capture methods and kits for differential isolation of nucleic acids including disease-associated sequences

Also Published As

Publication number Publication date
EP0981535A4 (de) 2000-11-29
JP2002502237A (ja) 2002-01-22
EP0981535A1 (de) 2000-03-01
WO1998051699A1 (en) 1998-11-19

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Legal Events

Date Code Title Description
FZDE Discontinued