DE1272627T1 - Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration - Google Patents

Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration

Info

Publication number
DE1272627T1
DE1272627T1 DE1272627T DE01922922T DE1272627T1 DE 1272627 T1 DE1272627 T1 DE 1272627T1 DE 1272627 T DE1272627 T DE 1272627T DE 01922922 T DE01922922 T DE 01922922T DE 1272627 T1 DE1272627 T1 DE 1272627T1
Authority
DE
Germany
Prior art keywords
ultrafiltration
plasmid
aid
determination
plasmid determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE1272627T
Other languages
English (en)
Inventor
Thacher Leonard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22727950&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE1272627(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Millipore Corp filed Critical Millipore Corp
Publication of DE1272627T1 publication Critical patent/DE1272627T1/de
Pending legal-status Critical Current

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14Ultrafiltration; Microfiltration
    • B01D61/18Apparatus therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14Ultrafiltration; Microfiltration
    • B01D61/145Ultrafiltration
    • B01D61/146Ultrafiltration comprising multiple ultrafiltration steps
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14Ultrafiltration; Microfiltration
    • B01D61/149Multistep processes comprising different kinds of membrane processes selected from ultrafiltration or microfiltration
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14Ultrafiltration; Microfiltration
    • B01D61/16Feed pretreatment
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2311/00Details relating to membrane separation process operations and control
    • B01D2311/04Specific process operations in the feed stream; Feed pretreatment
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Water Supply & Treatment (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE1272627T 2000-04-13 2001-03-29 Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration Pending DE1272627T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19707800P 2000-04-13 2000-04-13
PCT/US2001/010316 WO2001079486A2 (en) 2000-04-13 2001-03-29 Method and apparatus for plasmid recovery involving ultrafiltration

Publications (1)

Publication Number Publication Date
DE1272627T1 true DE1272627T1 (de) 2003-05-28

Family

ID=22727950

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60108950T Expired - Lifetime DE60108950T2 (de) 2000-04-13 2001-03-29 Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration
DE1272627T Pending DE1272627T1 (de) 2000-04-13 2001-03-29 Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60108950T Expired - Lifetime DE60108950T2 (de) 2000-04-13 2001-03-29 Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration

Country Status (6)

Country Link
US (2) US6759233B2 (de)
EP (1) EP1272627B1 (de)
JP (1) JP4150187B2 (de)
DE (2) DE60108950T2 (de)
ES (1) ES2184661T3 (de)
WO (1) WO2001079486A2 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1290158B1 (de) * 2000-06-02 2006-12-06 Pall Corporation Behandlung von plasmid-haltigen lösungen
US7074333B2 (en) 2001-01-19 2006-07-11 Millipore Corporation Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
US20030202909A1 (en) * 2002-04-29 2003-10-30 Genetix Limited Vacuum manifold and uses thereof
DE10238630A1 (de) * 2002-08-19 2004-03-04 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung biologischer Makromoleküle sowie Vorrichtung zur Durchführung dieses Verfahrens
US20040214180A1 (en) * 2003-04-28 2004-10-28 Hironori Kobayashi Method and kit for determining quantity of protein
JP4076978B2 (ja) 2003-11-20 2008-04-16 ミリポア・コーポレイション プラスミドdna清浄化
US7700369B2 (en) 2004-07-27 2010-04-20 Protedyne Corporation Method and apparatus for applying a pressure differential to a multi-well plate
US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
US8747669B1 (en) 2005-12-29 2014-06-10 Spf Innovations, Llc Method and apparatus for the filtration of biological samples
US7510654B2 (en) * 2005-12-29 2009-03-31 Spf Innovations, Llc Method and apparatus for the filtration of biological samples
WO2011156734A2 (en) 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
US10597652B2 (en) * 2011-03-29 2020-03-24 Phynexus, Inc. Methods and devices for nucleic acid purification
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
US10883100B2 (en) 2011-03-29 2021-01-05 Phynexus, Inc Devices and methods for plasmid purification
JP5823031B2 (ja) 2011-06-10 2015-11-25 日立化成株式会社 小胞捕捉デバイスおよびそれを用いるための方法
US9662649B2 (en) 2013-05-06 2017-05-30 Hitachi Chemical Company America, Ltd. Devices and methods for capturing target molecules
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
JP6631852B2 (ja) 2014-11-12 2020-01-15 日立化成株式会社 臓器障害を診断するための方法および装置
US11028443B2 (en) 2015-08-31 2021-06-08 Showa Denko Materials Co., Ltd. Molecular methods for assessing urothelial disease
BR112020020866A2 (pt) * 2018-04-13 2021-01-19 National Agricultural Genotyping Center Sonda de qpcr; composição de oligonucleotídeos de dna; método para detecção de quantificação de bactérias lactobacillus, pediococcus e weissella, ou uma ou alguma subcombinação das mesmas, em uma amostra de interesse, suspeita por conter ácido nucleico de lab; método de quantificação de bactérias lactobacillus, pediococcus e weissella, ou um ou uma subcombinação dos mesmos, em um meio biológico; método de preparo de dna de uma amostra de interesse para uma análise de pcr; aparelho de filtração; kit; e kit para obter uma extração de ácido nucleico de uma amostra que contém ácido nucleico de lab
WO2019239514A1 (ja) * 2018-06-13 2019-12-19 日立化成株式会社 細胞溶解装置および細胞溶解方法
WO2021198281A1 (en) 2020-03-31 2021-10-07 Richter-Helm Biologics Gmbh & Co. Kg Methods for producing plasmid dna
WO2024013278A1 (en) * 2022-07-13 2024-01-18 Dna Script Method of purifying solution comprising polynucleotide

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734192A (en) 1982-07-01 1988-03-29 Millipore Corporation Multiwell membrane filtration apparatus
US4948564A (en) 1986-10-28 1990-08-14 Costar Corporation Multi-well filter strip and composite assemblies
US5108704A (en) 1988-09-16 1992-04-28 W. R. Grace & Co.-Conn. Microfiltration apparatus with radially spaced nozzles
WO1992013963A1 (en) 1991-01-30 1992-08-20 Hyman Edward D Method for preparation of closed circular dna
US5116496A (en) 1991-03-19 1992-05-26 Minnesota Mining And Manufacturing Company Membrane-containing wells for microtitration and microfiltration
DE4139664A1 (de) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
CA2192262C (en) 1995-12-08 2011-03-15 Yoshihide Hayashizaki Method for purification and transfer to separation/detection systems of dna sequencing samples and plates used therefor
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US5665247A (en) 1996-09-16 1997-09-09 Whatman Inc. Process for sealing microplates utilizing a thin polymeric film
EP1025263A2 (de) * 1997-10-23 2000-08-09 GUILLET, James Edwin Markierung von polymeren und sequenzierung von nuleinsäuren
WO2000066723A1 (en) 1999-05-04 2000-11-09 Millipore Corporation Method of ultrafiltration

Also Published As

Publication number Publication date
ES2184661T1 (es) 2003-04-16
US20030203453A1 (en) 2003-10-30
US20020001836A1 (en) 2002-01-03
WO2001079486A3 (en) 2002-06-13
DE60108950T2 (de) 2006-02-23
DE60108950D1 (de) 2005-03-24
WO2001079486A2 (en) 2001-10-25
EP1272627A2 (de) 2003-01-08
US6759233B2 (en) 2004-07-06
EP1272627B1 (de) 2005-02-16
JP4150187B2 (ja) 2008-09-17
JP2003530854A (ja) 2003-10-21
US7297534B2 (en) 2007-11-20
ES2184661T3 (es) 2005-09-01

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