DE60108950D1 - Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration - Google Patents

Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration

Info

Publication number
DE60108950D1
DE60108950D1 DE60108950T DE60108950T DE60108950D1 DE 60108950 D1 DE60108950 D1 DE 60108950D1 DE 60108950 T DE60108950 T DE 60108950T DE 60108950 T DE60108950 T DE 60108950T DE 60108950 D1 DE60108950 D1 DE 60108950D1
Authority
DE
Germany
Prior art keywords
ultrafiltration
plasmid recovery
plasmid
recovery
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60108950T
Other languages
English (en)
Other versions
DE60108950T2 (de
Inventor
Thacher Leonard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22727950&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60108950(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Millipore Corp filed Critical Millipore Corp
Publication of DE60108950D1 publication Critical patent/DE60108950D1/de
Application granted granted Critical
Publication of DE60108950T2 publication Critical patent/DE60108950T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/145—Ultrafiltration
    • B01D61/146—Ultrafiltration comprising multiple ultrafiltration steps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/149—Multistep processes comprising different kinds of membrane processes selected from ultrafiltration or microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/16—Feed pretreatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/04—Specific process operations in the feed stream; Feed pretreatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Water Supply & Treatment (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60108950T 2000-04-13 2001-03-29 Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration Expired - Lifetime DE60108950T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19707800P 2000-04-13 2000-04-13
US197078P 2000-04-13
PCT/US2001/010316 WO2001079486A2 (en) 2000-04-13 2001-03-29 Method and apparatus for plasmid recovery involving ultrafiltration

Publications (2)

Publication Number Publication Date
DE60108950D1 true DE60108950D1 (de) 2005-03-24
DE60108950T2 DE60108950T2 (de) 2006-02-23

Family

ID=22727950

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60108950T Expired - Lifetime DE60108950T2 (de) 2000-04-13 2001-03-29 Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration
DE1272627T Pending DE1272627T1 (de) 2000-04-13 2001-03-29 Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE1272627T Pending DE1272627T1 (de) 2000-04-13 2001-03-29 Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration

Country Status (6)

Country Link
US (2) US6759233B2 (de)
EP (1) EP1272627B1 (de)
JP (1) JP4150187B2 (de)
DE (2) DE60108950T2 (de)
ES (1) ES2184661T3 (de)
WO (1) WO2001079486A2 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001094573A2 (en) * 2000-06-02 2001-12-13 Pall Corporation Processing of plasmid-containing fluids
US7074333B2 (en) * 2001-01-19 2006-07-11 Millipore Corporation Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
US20030202909A1 (en) * 2002-04-29 2003-10-30 Genetix Limited Vacuum manifold and uses thereof
DE10238630A1 (de) * 2002-08-19 2004-03-04 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung biologischer Makromoleküle sowie Vorrichtung zur Durchführung dieses Verfahrens
US20040214180A1 (en) * 2003-04-28 2004-10-28 Hironori Kobayashi Method and kit for determining quantity of protein
JP4076978B2 (ja) * 2003-11-20 2008-04-16 ミリポア・コーポレイション プラスミドｄｎａ清浄化
EP1789194B1 (de) 2004-07-27 2010-03-24 Protedyne Corporation Verfahren und vorrichtung zum anlegen eines druckdifferentialsan einer eine vielzahl von mulden aufweisenden platte
US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
US8747669B1 (en) 2005-12-29 2014-06-10 Spf Innovations, Llc Method and apparatus for the filtration of biological samples
US7384549B2 (en) * 2005-12-29 2008-06-10 Spf Innovations, Llc Method and apparatus for the filtration of biological solutions
WO2011156734A2 (en) 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
US10883100B2 (en) 2011-03-29 2021-01-05 Phynexus, Inc Devices and methods for plasmid purification
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
US10597652B2 (en) * 2011-03-29 2020-03-24 Phynexus, Inc. Methods and devices for nucleic acid purification
JP5823031B2 (ja) * 2011-06-10 2015-11-25 日立化成株式会社 小胞捕捉デバイスおよびそれを用いるための方法
DE212013000295U1 (de) 2013-05-06 2016-02-02 Hitachi Chemical Co. America, Ltd. Vorrichtungen zum Einfangen von Zielmolekülen
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
EP3218521B1 (de) 2014-11-12 2019-12-25 Hitachi Chemical Co., Ltd. Verfahren zur diagnose von organverletzung
DE112016003948T5 (de) 2015-08-31 2018-05-09 City Of Sapporo Molekulare verfahren zum beurteilen einer urothelialen erkrankung
US20210172004A1 (en) * 2018-04-13 2021-06-10 National Agricultural Genotyping Center Universal lactic acid bacteria quantification kit for fermentation monitoring
WO2019239514A1 (ja) * 2018-06-13 2019-12-19 日立化成株式会社 細胞溶解装置および細胞溶解方法
WO2021198281A1 (en) 2020-03-31 2021-10-07 Richter-Helm Biologics Gmbh & Co. Kg Methods for producing plasmid dna
WO2024013278A1 (en) * 2022-07-13 2024-01-18 Dna Script Method of purifying solution comprising polynucleotide

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734192A (en) 1982-07-01 1988-03-29 Millipore Corporation Multiwell membrane filtration apparatus
US4948564A (en) 1986-10-28 1990-08-14 Costar Corporation Multi-well filter strip and composite assemblies
US5108704A (en) 1988-09-16 1992-04-28 W. R. Grace & Co.-Conn. Microfiltration apparatus with radially spaced nozzles
WO1992013963A1 (en) 1991-01-30 1992-08-20 Hyman Edward D Method for preparation of closed circular dna
US5116496A (en) 1991-03-19 1992-05-26 Minnesota Mining And Manufacturing Company Membrane-containing wells for microtitration and microfiltration
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5856100A (en) 1995-12-08 1999-01-05 The Institute Of Physical And Chemical Research Method for purification and transfer to separation/detection systems of DNA sequencing samples and plates used therefor
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US5665247A (en) 1996-09-16 1997-09-09 Whatman Inc. Process for sealing microplates utilizing a thin polymeric film
AU9527098A (en) * 1997-10-23 1999-05-17 Nicholas A. D. Burke Labelling of polymers and sequencing of nucleic acids
WO2000066723A1 (en) 1999-05-04 2000-11-09 Millipore Corporation Method of ultrafiltration

Also Published As

Publication number Publication date
JP4150187B2 (ja) 2008-09-17
JP2003530854A (ja) 2003-10-21
EP1272627A2 (de) 2003-01-08
US6759233B2 (en) 2004-07-06
US20030203453A1 (en) 2003-10-30
WO2001079486A2 (en) 2001-10-25
WO2001079486A3 (en) 2002-06-13
ES2184661T3 (es) 2005-09-01
DE60108950T2 (de) 2006-02-23
DE1272627T1 (de) 2003-05-28
ES2184661T1 (es) 2003-04-16
US7297534B2 (en) 2007-11-20
US20020001836A1 (en) 2002-01-03
EP1272627B1 (de) 2005-02-16

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