ATE176278T1 - Gerät und methoden zur elektroporation und elektrofusion - Google Patents

Gerät und methoden zur elektroporation und elektrofusion

Info

Publication number
ATE176278T1
ATE176278T1 AT92916662T AT92916662T ATE176278T1 AT E176278 T1 ATE176278 T1 AT E176278T1 AT 92916662 T AT92916662 T AT 92916662T AT 92916662 T AT92916662 T AT 92916662T AT E176278 T1 ATE176278 T1 AT E176278T1
Authority
AT
Austria
Prior art keywords
cells
type
pores
electrofusion
electroporation
Prior art date
Application number
AT92916662T
Other languages
English (en)
Inventor
Robert E Schmukler
Original Assignee
Robert E Schmukler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert E Schmukler filed Critical Robert E Schmukler
Application granted granted Critical
Publication of ATE176278T1 publication Critical patent/ATE176278T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Electromagnetism (AREA)
  • Biophysics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Branch Pipes, Bends, And The Like (AREA)
  • Lining Or Joining Of Plastics Or The Like (AREA)
  • Vaporization, Distillation, Condensation, Sublimation, And Cold Traps (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
AT92916662T 1991-07-22 1992-07-21 Gerät und methoden zur elektroporation und elektrofusion ATE176278T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/733,854 US5173158A (en) 1991-07-22 1991-07-22 Apparatus and methods for electroporation and electrofusion

Publications (1)

Publication Number Publication Date
ATE176278T1 true ATE176278T1 (de) 1999-02-15

Family

ID=24949376

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92916662T ATE176278T1 (de) 1991-07-22 1992-07-21 Gerät und methoden zur elektroporation und elektrofusion

Country Status (7)

Country Link
US (2) US5173158A (de)
EP (1) EP0596003B1 (de)
AT (1) ATE176278T1 (de)
AU (1) AU2378092A (de)
CA (1) CA2113907C (de)
DE (1) DE69228309T2 (de)
WO (1) WO1993002178A1 (de)

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CA2113907A1 (en) 1993-02-04
EP0596003B1 (de) 1999-01-27
WO1993002178A1 (en) 1993-02-04
DE69228309T2 (de) 1999-09-16
CA2113907C (en) 2002-12-24
AU2378092A (en) 1993-02-23
US5173158A (en) 1992-12-22
DE69228309D1 (de) 1999-03-11
EP0596003A4 (en) 1995-12-06
US5283194A (en) 1994-02-01

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