ATE170980T1 - Bestimmung von analyten durch übertragung von fluoreszenzenergie - Google Patents

Bestimmung von analyten durch übertragung von fluoreszenzenergie

Info

Publication number
ATE170980T1
ATE170980T1 AT91913255T AT91913255T ATE170980T1 AT E170980 T1 ATE170980 T1 AT E170980T1 AT 91913255 T AT91913255 T AT 91913255T AT 91913255 T AT91913255 T AT 91913255T AT E170980 T1 ATE170980 T1 AT E170980T1
Authority
AT
Austria
Prior art keywords
concentration
analytes
fluorochromes
fluorochrome
camp
Prior art date
Application number
AT91913255T
Other languages
English (en)
Inventor
Roger Y Tsien
Susan S Taylor
Stephen R Adams
Ying Ji
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Application granted granted Critical
Publication of ATE170980T1 publication Critical patent/ATE170980T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/80Fluorescent dyes, e.g. rhodamine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Holo Graphy (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT91913255T 1990-07-02 1991-07-01 Bestimmung von analyten durch übertragung von fluoreszenzenergie ATE170980T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US54799090A 1990-07-02 1990-07-02

Publications (1)

Publication Number Publication Date
ATE170980T1 true ATE170980T1 (de) 1998-09-15

Family

ID=24186980

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91913255T ATE170980T1 (de) 1990-07-02 1991-07-01 Bestimmung von analyten durch übertragung von fluoreszenzenergie

Country Status (7)

Country Link
US (1) US5439797A (de)
EP (1) EP0537270B1 (de)
JP (1) JP3208486B2 (de)
AT (1) ATE170980T1 (de)
DE (1) DE69130171T2 (de)
DK (1) DK0537270T3 (de)
WO (1) WO1992000388A1 (de)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9310978D0 (en) * 1993-05-27 1993-07-14 Zeneca Ltd Compounds
US6482655B1 (en) * 1993-07-23 2002-11-19 University Of Utah Research Foundation Immunoassay procedure utilizing fluorogenic tracer antigens
US5861124A (en) * 1993-07-15 1999-01-19 Hamamatsu Photonics Kk Method and apparatus for detecting denaturation of nucleic acid
US6576419B1 (en) 1993-07-23 2003-06-10 University Of Utah Research Foundation Assay procedure using fluorogenic tracers
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US6766183B2 (en) 1995-11-22 2004-07-20 Medtronic Minimed, Inc. Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7825237B2 (en) 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5814449A (en) * 1996-05-28 1998-09-29 University Of Pittsburgh Homogenous affinity assay for quantitative drug and metabolite determination
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
ATE290205T1 (de) 1996-12-12 2005-03-15 Prolume Ltd Vorrichtung und verfahren zum nachweis und identifizieren von infektiösen wirkstoffen
US6197928B1 (en) * 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
WO1998040477A1 (en) * 1997-03-14 1998-09-17 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
FR2764387B1 (fr) * 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
GB9718358D0 (en) * 1997-08-30 1997-11-05 Univ Leeds Chemical modification
FR2768817B1 (fr) 1997-09-19 1999-12-10 Cis Bio Int Methode homogene pour la detection et/ou la determination de l'activite phosphorylante d'un materiel biologique
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AU1366299A (en) * 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
CA2324648C (en) 1998-03-27 2013-02-26 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
GB9904401D0 (en) * 1999-02-25 1999-04-21 Fluorescience Ltd Assay method
DE60015319T2 (de) * 1999-02-25 2005-10-27 Cyclacel Ltd. Test zur gleichzeitigen Messung von verschiedenen enzymischen Aktivitäten
US7166475B2 (en) * 1999-02-26 2007-01-23 Cyclacel Ltd. Compositions and methods for monitoring the modification state of a pair of polypeptides
US6972182B1 (en) * 1999-02-26 2005-12-06 Cyclacel, Ltd. Methods and compositions using coiled binding partners
US6828106B2 (en) * 1999-02-26 2004-12-07 Cyclacel Limited Methods and compositions using coiled binding partners
US6656696B2 (en) 1999-02-26 2003-12-02 Cyclacel Compositions and methods for monitoring the phosphorylation of natural binding partners
US6670144B1 (en) 1999-02-26 2003-12-30 Cyclacel, Ltd. Compositions and methods for monitoring the phosphorylation of natural binding partners
US6699687B1 (en) 1999-05-21 2004-03-02 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US6469154B1 (en) 1999-05-21 2002-10-22 The Regents Of The University Of California Fluorescent protein indicators
US7060793B2 (en) * 1999-05-21 2006-06-13 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
CA2369855A1 (en) * 1999-05-28 2001-01-04 Sangamo Biosciences, Inc. Molecular switches
US6573059B1 (en) 1999-06-04 2003-06-03 Rmf Dictagene S.A. Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements
EP1206699A4 (de) * 1999-07-27 2005-01-19 Univ Utah Res Found Homogenes fluoreszenzverfahren zum bestimmen struktureller modifikationen von biomolekülen
US6358684B1 (en) 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
US7332275B2 (en) 1999-10-13 2008-02-19 Sequenom, Inc. Methods for detecting methylated nucleotides
JP2003527833A (ja) 1999-10-14 2003-09-24 クロンテック・ラボラトリーズ・インコーポレーテッド 花虫類に由来する発色団/蛍光体、およびそれらの使用法
US20040214227A1 (en) * 1999-12-22 2004-10-28 Erik Joly Bioluminescence resonance energy transfer (bret) fusion molecule and method of use
WO2001053479A2 (en) * 2000-01-24 2001-07-26 Sangamo Biosciences, Inc. Molecular switches ii
US7060506B2 (en) 2000-01-31 2006-06-13 Cyclacel, Ltd. Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US6808874B2 (en) 2000-06-13 2004-10-26 Cyclacel Ltd. Methods of monitoring enzyme activity
AU2002251944A1 (en) * 2001-02-15 2002-09-04 Medtronic Minimed, Inc. Polymers functionalized with fluorescent boronate motifs
WO2002093129A2 (en) * 2001-05-15 2002-11-21 University Of Medicine & Dentistry Of New Jersey Methods for analyzing interactions between proteins in live and intact cells
JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
US7045361B2 (en) 2001-09-12 2006-05-16 Medtronic Minimed, Inc. Analyte sensing via acridine-based boronate biosensors
CA2462617A1 (en) * 2001-11-07 2003-05-15 Sensor Technologies Llc Method of identifying energy transfer sensors for analytes
US6972326B2 (en) * 2001-12-03 2005-12-06 Molecular Probes, Inc. Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes
AU2002357322A1 (en) * 2001-12-19 2003-07-09 The University Of Chicago Rapidly maturing fluorescent proteins and methods for using the same
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
CA2484676A1 (en) 2002-05-03 2003-11-13 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof, and related methods
US7195884B2 (en) * 2002-07-19 2007-03-27 Promega Corp. Methods and kits for transferases
US7268878B2 (en) * 2002-08-01 2007-09-11 Sensor Technologies Llc Fluorescence correlation spectroscopy instrument and method of using the same
US7491545B2 (en) * 2002-10-01 2009-02-17 Richard Thompson Excitation ratiometric fluoroscent biosensor for zinc ion at picomolar levels
ATE509946T1 (de) 2002-11-12 2011-06-15 Zakrytoe Aktsionernoe Obschestvo S Evrogens Fluoreszenzproteine und chromoproteine aus nicht- aequorea-hydrozoa-spezies sowie verfahren zur verwendung davon
ATE474922T1 (de) 2002-12-26 2010-08-15 Zakrytoe Aktsionernoe Obschest Fluoreszenzproteine aus copepoda-spezies und verfahren zur verwendung davon
US8426194B2 (en) 2003-01-21 2013-04-23 Ptc Therapeutics, Inc. Methods and agents for screening for compounds capable of modulating VEGF expression
US9068234B2 (en) 2003-01-21 2015-06-30 Ptc Therapeutics, Inc. Methods and agents for screening for compounds capable of modulating gene expression
CA2514184C (en) 2003-01-21 2016-04-12 Ptc Therapeutics, Inc. Methods for identifying compounds that modulate untranslated region-dependent gene expression and methods of using same
EP1613159A4 (de) * 2003-03-27 2007-08-01 Ptc Therapeutics Inc Verfahren zur identifikation von verbindungen mit einer trna-splicenden endonuklease als angriffsort, sowie verwendungen für diese verbindungen als antifungale mittel
EP2363025A1 (de) 2003-03-27 2011-09-07 PTC Therapeutics, Inc. Betrachtung von Enzymen des TRNA-Splice-Wegs zur Identifikation von Antifungalen und/oder Proliferationshemmenden Molekülen
CA2520415A1 (en) * 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. Methods of identifying compounds that target trna splicing endonuclease and uses of said compounds as anti-proliferative agents
WO2005003316A2 (en) * 2003-07-02 2005-01-13 Ptc Therapeutics, Inc. Rna processing protein complexes and uses thereof
US20050095174A1 (en) * 2003-10-31 2005-05-05 Wolf David E. Semipermeable sensors for detecting analyte
US20070072186A1 (en) * 2003-11-17 2007-03-29 Anuradha Mehta Methods and agents for screening for compounds capable of modulating her2 expression
EP1732944B1 (de) * 2004-04-07 2012-09-05 The University of Chicago Monomere, rotfluoreszierende proteine
US7465810B2 (en) * 2004-10-25 2008-12-16 Anaspec, Inc. Reactive 1,3′-crosslinked carbocyanine
WO2007052102A2 (en) * 2005-11-04 2007-05-10 Evrogen, Jsc Modified green fluorescent proteins and methods for using same
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
DK1994149T3 (da) * 2006-01-25 2011-01-17 Evrogen Ip Nye fluorescerende proteiner og fremgangsmåder til anvendelse deraf
WO2008094316A2 (en) * 2006-09-22 2008-08-07 Stowers Institute Of Medical Research Novel branchiostoma derived fluorescent proteins
DE102007017051A1 (de) * 2007-04-11 2008-10-16 Humboldt-Universität Zu Berlin Dual-markierte Fluoreszenzsonden zur Detektion von Proteinen
US8283115B1 (en) 2007-06-20 2012-10-09 Ptc Therapeutics, Inc. Methods of screening for compounds for treating muscular dystrophy using UTRN mRNA translation regulation
US8283116B1 (en) 2007-06-22 2012-10-09 Ptc Therapeutics, Inc. Methods of screening for compounds for treating spinal muscular atrophy using SMN mRNA translation regulation
WO2009059305A2 (en) * 2007-11-01 2009-05-07 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
EP2615111A1 (de) 2012-01-10 2013-07-17 Laboratorios del Dr. Esteve S.A. Verfahren zur Identifizierung von Liganden für Sigma-1-Rezeptoren
CN105062465B (zh) * 2015-07-31 2018-06-15 山东大学 一类环境敏感型的α1-肾上腺素能受体近红外荧光配体及其应用
PT109877A (pt) * 2017-01-26 2018-07-26 Inst Superior Tecnico Método ótico para a medição da concentração de oxigénio em sistemas de combustível.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828981A (en) * 1983-08-24 1989-05-09 Synbiotics Corporation Immunoassays for determining Dirofilaria immitis infection using antiidiotype monoclonal antibody reagents
US4822733A (en) * 1985-05-28 1989-04-18 Amoco Corporation Lifetime-resolved assay procedures
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer

Also Published As

Publication number Publication date
DE69130171D1 (de) 1998-10-15
EP0537270A4 (en) 1996-07-24
DK0537270T3 (da) 1999-06-07
WO1992000388A1 (en) 1992-01-09
EP0537270A1 (de) 1993-04-21
EP0537270B1 (de) 1998-09-09
DE69130171T2 (de) 1999-06-24
JPH05508772A (ja) 1993-12-09
US5439797A (en) 1995-08-08
JP3208486B2 (ja) 2001-09-10

Similar Documents

Publication Publication Date Title
ATE170980T1 (de) Bestimmung von analyten durch übertragung von fluoreszenzenergie
Chang et al. Aurovertin, a fluorescent probe of conformational change in beef heart mitochondrial adenosine triphosphatase
Huber et al. Dual lifetime referencing as applied to a chloride optical sensor
Mayer et al. Dissolved protein fluorescence in two Maine estuaries
Huber et al. Optical sensor for seawater salinity
Kühl et al. Combined imaging of bacteria and oxygen in biofilms
Lakowicz et al. Fluorophores
Vasylevska et al. Indicator-loaded permeation-selective microbeads for use in fiber optic simultaneous sensing of pH and dissolved oxygen
Fábián et al. Strength in numbers: effects of acceptor abundance on FRET efficiency
US6197258B1 (en) Photoluminescent sensors of chemical analytes
DE69716454D1 (de) Nachweisverfahren mit hilfe von fluoreszent-energietransferliganden auf lipidfilmen
Liu et al. An array fluorescent biosensor based on planar waveguide for multi-analyte determination in water samples
DE69840146D1 (de) Fluoreszenzprotein-sensoren zur erkennung von analyten
DE50115064D1 (de) Sensor zur lumineszenz-optischen bestimmung eines analyten
Kirillova et al. Effect of halogenated fluorescent compounds on bioluminescent reactions
Popov et al. Multiwavelength method for measuring concentration of free cytosolic calcium using the fluorescent probe indo-1
Turel et al. Direct UV-LED lifetime pH sensor based on a semi-permeable sol–gel membrane immobilized luminescent Eu3+ chelate complex
Baker et al. Extending the reach of immunoassays to optically dense specimens by using two-photon excited fluorescence polarization
Sipior et al. Phase fluorometric optical carbon dioxide gas sensor for fermentation off‐gas monitoring
Nieva-Gomez et al. Membrane changes in Escherichia coli induced by colicin Ia and agents known to disrupt energy transduction
Huber et al. Nitrate-selective optical sensor applying a lipophilic fluorescent potential-sensitive dye
Strömberg et al. A fluorescence ratiometric detection scheme for ammonium ions based on the solvent sensitive dye MC 540
Thompson et al. Zinc biosensing with multiphoton excitation using carbonic anhydrase and improved fluorophores
US20140273038A1 (en) Bioluminescent metal ion assay
Papkovsky et al. Fibre-optic lifetime-based enzyme biosensor

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties