AR126213A1 - Microorganismo recombinante con expresión regulada de nadh:quinona oxidorreductasa y método para producir o-fosfoserina, cisteína y derivados de la misma que utiliza la misma - Google Patents

Microorganismo recombinante con expresión regulada de nadh:quinona oxidorreductasa y método para producir o-fosfoserina, cisteína y derivados de la misma que utiliza la misma

Info

Publication number
AR126213A1
AR126213A1 ARP220101649A ARP220101649A AR126213A1 AR 126213 A1 AR126213 A1 AR 126213A1 AR P220101649 A ARP220101649 A AR P220101649A AR P220101649 A ARP220101649 A AR P220101649A AR 126213 A1 AR126213 A1 AR 126213A1
Authority
AR
Argentina
Prior art keywords
phosphoserine
same
producing
cysteine
nadh
Prior art date
Application number
ARP220101649A
Other languages
English (en)
Inventor
Hwi Jung
- Park Hye Min Min
Hee Sim
- Lee Jin Nam Jin
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of AR126213A1 publication Critical patent/AR126213A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)
    • C12Y106/99005—NADH dehydrogenase (quinone) (1.6.99.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01065—O-Phosphoserine sulfhydrylase (2.5.1.65)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03003—Phosphoserine phosphatase (3.1.3.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente solicitud se refiere a un microorganismo recombinante, en el que se regula la expresión de NADH:quinona oxidorreductasa, y un método para producir O-fosfoserina, cisteína, y derivados de cisteína que utilizan la misma. Reivindicación 1: Un microorganismo recombinante del género Escherichia que tiene una actividad de NADH:quinona oxidorreductasa potenciada y una capacidad de producción de O-fosfoserina. Reivindicación 10: Un método para producir cisteína o un derivado de la misma, que comprende: a) cultivar un microorganismo que produce O-fosfoserina con una actividad de NADH:quinona oxidorreductasa potenciada en un medio para producir O-fosfoserina o un medio que contiene la misma; y b) hacer reaccionar la O-fosfoserina producida en la etapa a) o un medio que contiene la misma con un sulfuro en la presencia de O-fosfoserina sulfhidrilasa (OPSS) o un microorganismo que expresa la misma.
ARP220101649A 2021-06-23 2022-06-23 Microorganismo recombinante con expresión regulada de nadh:quinona oxidorreductasa y método para producir o-fosfoserina, cisteína y derivados de la misma que utiliza la misma AR126213A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020210081785A KR102654301B1 (ko) 2021-06-23 2021-06-23 NADH:quinone 산화환원효소의 발현이 조절된 재조합 미생물 및 이를 이용한 O-포스포세린, 시스테인 및 이의 유도체의 생산방법

Publications (1)

Publication Number Publication Date
AR126213A1 true AR126213A1 (es) 2023-09-27

Family

ID=84538605

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220101649A AR126213A1 (es) 2021-06-23 2022-06-23 Microorganismo recombinante con expresión regulada de nadh:quinona oxidorreductasa y método para producir o-fosfoserina, cisteína y derivados de la misma que utiliza la misma

Country Status (10)

Country Link
EP (1) EP4361276A1 (es)
JP (1) JP2024522877A (es)
KR (1) KR102654301B1 (es)
CN (1) CN117897489A (es)
AR (1) AR126213A1 (es)
AU (1) AU2022297143A1 (es)
BR (1) BR112023027397A2 (es)
CA (1) CA3223970A1 (es)
MX (1) MX2024000244A (es)
WO (1) WO2022270857A1 (es)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19726083A1 (de) 1997-06-19 1998-12-24 Consortium Elektrochem Ind Mikroorganismen und Verfahren zur fermentativen Herstellung von L-Cystein, L-Cystin, N-Acetyl-Serin oder Thiazolidinderivaten
RU2275425C2 (ru) * 2003-11-03 2006-04-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина и способ получения l-цистеина
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
KR101208267B1 (ko) 2010-10-20 2012-12-04 씨제이제일제당 (주) Ｏ-포스포세린 설피드릴라제 변이체
KR101381048B1 (ko) 2010-10-20 2014-04-14 씨제이제일제당 (주) Ｏ-포스포세린 생산 균주 및 이로부터 생산된 ｏ-포스포세린으로부터 ｌ-시스테인 또는 이의 유도체의 생산방법
KR101547651B1 (ko) 2014-06-03 2015-08-26 씨제이제일제당 주식회사 O-숙시닐호모세린 또는 숙신산의 생산능을 갖는 미생물 및 이를 이용한 숙신산 또는 o-숙시닐호모세린의 생산 방법
KR101677328B1 (ko) 2014-08-12 2016-11-18 씨제이제일제당 (주) O-포스포세린 생산 미생물 및 이를 이용한 o-포스포세린 또는 l-시스테인 생산 방법
KR101694632B1 (ko) * 2015-09-11 2017-01-10 씨제이제일제당 (주) 신규 ｏ-포스포세린 배출 단백질 변이체 및 이를 이용한 ｏ-포스포세린, 시스테인 및 이의 유도체의 생산방법
WO2020032590A1 (en) 2018-08-07 2020-02-13 Cj Cheiljedang Corporation Nucleic acid molecules comprising a variant rpoc coding sequence

Also Published As

Publication number Publication date
CA3223970A1 (en) 2022-12-29
EP4361276A1 (en) 2024-05-01
BR112023027397A2 (pt) 2024-03-12
MX2024000244A (es) 2024-01-31
TW202313963A (zh) 2023-04-01
KR102654301B1 (ko) 2024-04-04
WO2022270857A1 (ko) 2022-12-29
AU2022297143A1 (en) 2024-01-18
CN117897489A (zh) 2024-04-16
KR20220170657A (ko) 2022-12-30
JP2024522877A (ja) 2024-06-21

Similar Documents

Publication Publication Date Title
AR121142A1 (es) Microorganismo que comprende un lyse mutado y método de producción de un l-aminoácido que lo utiliza
ES2585477T3 (es) Células modificadas metabólicamente para la producción de pinosilvina
BR0303461A (pt) Bactéria que produz l-aminoácido pertencente ao gênero escherichia, e, método para a produção de l-aminoácido
PE20100095A1 (es) Microorganismo productor del acido l-glutamico y metodo para producir acido l-glutamico
BRPI0619325B8 (pt) célula hospedeira microbiana recombinante e método para produção de isobutanol
AR068722A1 (es) Metodo para la produccion de metionina, sus derivados o precursores a partir de un proceso fermentativo
AR102049A1 (es) Microorganismo que produce o-fosfoserina y un procedimiento para la producción de o-fosfoserina o l-cisteína por el uso del mismo
CR7686A (es) Suplemento agricola seguro para el ambiente
BR112023005104A2 (pt) Micro-organismo wood-ljungdahl geneticamente modificado, e, método para aumentar a produção de um produto
MX2020007037A (es) Un microorganismo del genero corynebacterium que produce l-aminoacidos y un procedimiento para producir l-aminoacidos mediante el uso del mismo.
BR112019006926A8 (pt) Método para cultivar bactérias que formam esporo, e método para produzir substância útil
AR126213A1 (es) Microorganismo recombinante con expresión regulada de nadh:quinona oxidorreductasa y método para producir o-fosfoserina, cisteína y derivados de la misma que utiliza la misma
PE20200291A1 (es) Glicosidos de esteviol de alta pureza
BR0201520A (pt) Método para produzir uma subst ncia de marcação utilizando um microorganismo
AR078938A1 (es) Caldo de fermentacion de actinomicetos con actividad insecticida
NO20053205L (no) Forbedret fremgangsmate for produksjon av vitamin B12.
AR123450A1 (es) Un microorganismo recombinante para producir ácido l-glutámico y un método para producir ácido l-glutámico utilizando el mismo
PH12021550347A1 (en) MICROORGANISM FOR PRODUCING L-AMINO ACID WITH ENHANCED ACTIVITY OF a-GLUCOSIDASE AND METHOD FOR PRODUCING L-AMINO ACID USING THE SAME
AR120516A1 (es) Microorganismo y método para producir ergotioneína
DE60313631D1 (de) Produktion von zeaxanthin und beta-cryptoxanthin durch phaffia
ZA202206645B (en) Novel promoter and method of producing glutathione using the same
BR112023020933A2 (pt) Organismo geneticamente modificado, gene de retinol desidrogenase (rdh12), vetor de expressão, célula hospedeira, retinol desidrogenase 12 (rdh12), biofilme, biorreator e reator de biofilme, e método para produzir um isoprenoide em um biorreator de biofilme
BR112023014312A2 (pt) Método para produzir 2,4-di-hidroxibutirato (dhb) ou l-treonina com o uso de uma via metabólica microbiana, enzima com atividade de 2-ceto-4-hidroxibutirato (ohb) redutase que catalisa a conversão de 2-ceto-4-hidroxibutirato (ohb) em 2,4-di-hidroxibutirato (dhb) e uso de uma enzima
AR126071A1 (es) Nueva variante de yhhs y método para producir o-fosfoselina, cistina y derivado de cistina usando la misma
BR112023003780A2 (pt) Microrganismo produtor de l-valina e método para produzir l-valina usando o mesmo