AR126213A1 - Microorganismo recombinante con expresión regulada de nadh:quinona oxidorreductasa y método para producir o-fosfoserina, cisteína y derivados de la misma que utiliza la misma - Google Patents
Microorganismo recombinante con expresión regulada de nadh:quinona oxidorreductasa y método para producir o-fosfoserina, cisteína y derivados de la misma que utiliza la mismaInfo
- Publication number
- AR126213A1 AR126213A1 ARP220101649A ARP220101649A AR126213A1 AR 126213 A1 AR126213 A1 AR 126213A1 AR P220101649 A ARP220101649 A AR P220101649A AR P220101649 A ARP220101649 A AR P220101649A AR 126213 A1 AR126213 A1 AR 126213A1
- Authority
- AR
- Argentina
- Prior art keywords
- phosphoserine
- same
- producing
- cysteine
- nadh
- Prior art date
Links
- BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 title abstract 6
- 244000005700 microbiome Species 0.000 title abstract 5
- 101710157142 2-methylene-furan-3-one reductase Proteins 0.000 title abstract 4
- 101710189291 Quinone oxidoreductase Proteins 0.000 title abstract 4
- 102100034576 Quinone oxidoreductase Human genes 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 title abstract 3
- 235000018417 cysteine Nutrition 0.000 title abstract 3
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 title abstract 2
- 230000001105 regulatory effect Effects 0.000 title abstract 2
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 abstract 3
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 3
- 108030001910 O-phosphoserine sulfhydrylases Proteins 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 241000588722 Escherichia Species 0.000 abstract 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 abstract 1
- 150000001944 cysteine derivatives Chemical class 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/12—Methionine; Cysteine; Cystine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)
- C12Y106/99005—NADH dehydrogenase (quinone) (1.6.99.5)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01065—O-Phosphoserine sulfhydrylase (2.5.1.65)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
- C12Y301/03003—Phosphoserine phosphatase (3.1.3.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente solicitud se refiere a un microorganismo recombinante, en el que se regula la expresión de NADH:quinona oxidorreductasa, y un método para producir O-fosfoserina, cisteína, y derivados de cisteína que utilizan la misma. Reivindicación 1: Un microorganismo recombinante del género Escherichia que tiene una actividad de NADH:quinona oxidorreductasa potenciada y una capacidad de producción de O-fosfoserina. Reivindicación 10: Un método para producir cisteína o un derivado de la misma, que comprende: a) cultivar un microorganismo que produce O-fosfoserina con una actividad de NADH:quinona oxidorreductasa potenciada en un medio para producir O-fosfoserina o un medio que contiene la misma; y b) hacer reaccionar la O-fosfoserina producida en la etapa a) o un medio que contiene la misma con un sulfuro en la presencia de O-fosfoserina sulfhidrilasa (OPSS) o un microorganismo que expresa la misma.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020210081785A KR102654301B1 (ko) | 2021-06-23 | 2021-06-23 | NADH:quinone 산화환원효소의 발현이 조절된 재조합 미생물 및 이를 이용한 O-포스포세린, 시스테인 및 이의 유도체의 생산방법 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR126213A1 true AR126213A1 (es) | 2023-09-27 |
Family
ID=84538605
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP220101649A AR126213A1 (es) | 2021-06-23 | 2022-06-23 | Microorganismo recombinante con expresión regulada de nadh:quinona oxidorreductasa y método para producir o-fosfoserina, cisteína y derivados de la misma que utiliza la misma |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP4361276A1 (es) |
JP (1) | JP2024522877A (es) |
KR (1) | KR102654301B1 (es) |
CN (1) | CN117897489A (es) |
AR (1) | AR126213A1 (es) |
AU (1) | AU2022297143A1 (es) |
BR (1) | BR112023027397A2 (es) |
CA (1) | CA3223970A1 (es) |
MX (1) | MX2024000244A (es) |
WO (1) | WO2022270857A1 (es) |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19726083A1 (de) | 1997-06-19 | 1998-12-24 | Consortium Elektrochem Ind | Mikroorganismen und Verfahren zur fermentativen Herstellung von L-Cystein, L-Cystin, N-Acetyl-Serin oder Thiazolidinderivaten |
RU2275425C2 (ru) * | 2003-11-03 | 2006-04-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина и способ получения l-цистеина |
KR100620092B1 (ko) | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
KR101208267B1 (ko) | 2010-10-20 | 2012-12-04 | 씨제이제일제당 (주) | O-포스포세린 설피드릴라제 변이체 |
KR101381048B1 (ko) | 2010-10-20 | 2014-04-14 | 씨제이제일제당 (주) | O-포스포세린 생산 균주 및 이로부터 생산된 o-포스포세린으로부터 l-시스테인 또는 이의 유도체의 생산방법 |
KR101547651B1 (ko) | 2014-06-03 | 2015-08-26 | 씨제이제일제당 주식회사 | O-숙시닐호모세린 또는 숙신산의 생산능을 갖는 미생물 및 이를 이용한 숙신산 또는 o-숙시닐호모세린의 생산 방법 |
KR101677328B1 (ko) | 2014-08-12 | 2016-11-18 | 씨제이제일제당 (주) | O-포스포세린 생산 미생물 및 이를 이용한 o-포스포세린 또는 l-시스테인 생산 방법 |
KR101694632B1 (ko) * | 2015-09-11 | 2017-01-10 | 씨제이제일제당 (주) | 신규 o-포스포세린 배출 단백질 변이체 및 이를 이용한 o-포스포세린, 시스테인 및 이의 유도체의 생산방법 |
WO2020032590A1 (en) | 2018-08-07 | 2020-02-13 | Cj Cheiljedang Corporation | Nucleic acid molecules comprising a variant rpoc coding sequence |
-
2021
- 2021-06-23 KR KR1020210081785A patent/KR102654301B1/ko active IP Right Grant
-
2022
- 2022-06-21 JP JP2023579425A patent/JP2024522877A/ja active Pending
- 2022-06-21 WO PCT/KR2022/008745 patent/WO2022270857A1/ko active Application Filing
- 2022-06-21 EP EP22828704.1A patent/EP4361276A1/en active Pending
- 2022-06-21 MX MX2024000244A patent/MX2024000244A/es unknown
- 2022-06-21 CN CN202280057073.7A patent/CN117897489A/zh active Pending
- 2022-06-21 AU AU2022297143A patent/AU2022297143A1/en active Pending
- 2022-06-21 BR BR112023027397A patent/BR112023027397A2/pt unknown
- 2022-06-21 CA CA3223970A patent/CA3223970A1/en active Pending
- 2022-06-23 AR ARP220101649A patent/AR126213A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
CA3223970A1 (en) | 2022-12-29 |
EP4361276A1 (en) | 2024-05-01 |
BR112023027397A2 (pt) | 2024-03-12 |
MX2024000244A (es) | 2024-01-31 |
TW202313963A (zh) | 2023-04-01 |
KR102654301B1 (ko) | 2024-04-04 |
WO2022270857A1 (ko) | 2022-12-29 |
AU2022297143A1 (en) | 2024-01-18 |
CN117897489A (zh) | 2024-04-16 |
KR20220170657A (ko) | 2022-12-30 |
JP2024522877A (ja) | 2024-06-21 |
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