AR093293A1 - Polinucleotidos, polipeptidos de aciltransferasa y sus metodos de uso - Google Patents

Polinucleotidos, polipeptidos de aciltransferasa y sus metodos de uso

Info

Publication number
AR093293A1
AR093293A1 ARP130103960A ARP130103960A AR093293A1 AR 093293 A1 AR093293 A1 AR 093293A1 AR P130103960 A ARP130103960 A AR P130103960A AR P130103960 A ARP130103960 A AR P130103960A AR 093293 A1 AR093293 A1 AR 093293A1
Authority
AR
Argentina
Prior art keywords
dgat1
application
plants
methods
cells
Prior art date
Application number
ARP130103960A
Other languages
English (en)
Original Assignee
Agresearch Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agresearch Ltd filed Critical Agresearch Ltd
Publication of AR093293A1 publication Critical patent/AR093293A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)

Abstract

La solicitud proporciona una proteína DGAT1 (enzima formadora de triacilglicerol) con variadas propiedades respecto de las proteínas DGAT1 conocidas, en particular proteínas DGAT1 conocidas de plantas. La proteína DGAT1 de la solicitud se puede expresar en las células para aumentar la acumulación de lípidos celulares. La expresión de la proteína DGAT1 de la solicitud en las células produce un mayor nivel de lípidos que cualquiera de diversas otras proteínas DGAT1 de plantas analizadas por los solicitantes. La solicitud proporciona polinucleótidos que codifica la proteína DGAT1 de SEQ ID NO:39, constructos, células, planta, parte de plantas y progenie que comprende los polinucleótidos, y métodos de uso de los polinucleótidos y polipéptido de la solicitud.
ARP130103960A 2012-10-30 2013-10-30 Polinucleotidos, polipeptidos de aciltransferasa y sus metodos de uso AR093293A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261720136P 2012-10-30 2012-10-30

Publications (1)

Publication Number Publication Date
AR093293A1 true AR093293A1 (es) 2015-05-27

Family

ID=50628189

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130103960A AR093293A1 (es) 2012-10-30 2013-10-30 Polinucleotidos, polipeptidos de aciltransferasa y sus metodos de uso

Country Status (11)

Country Link
US (1) US9957519B2 (es)
EP (1) EP2914725A4 (es)
CN (1) CN105121646B (es)
AR (1) AR093293A1 (es)
AU (1) AU2013340444B2 (es)
BR (1) BR112015009303A2 (es)
CA (1) CA2889980A1 (es)
IN (1) IN2015DN03967A (es)
MX (1) MX359167B (es)
NZ (1) NZ706829A (es)
WO (1) WO2014068438A2 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2015DN04034A (es) * 2012-10-30 2015-10-02 Agres Ltd
PL2914726T3 (pl) 2012-10-30 2019-06-28 Agresearch Limited Udoskonalone polinukleotydy acylotransferazy, polipeptydy i sposoby stosowania
US9648813B2 (en) 2012-12-21 2017-05-16 The New Zealand Institute For Plant And Food Research Limited Regulation of gene expression via uORF of GDP-L-galactose phosphorylase (GGP) gene
BR112016030971B1 (pt) 2014-07-07 2022-09-27 Commonwealth Scientific And Industrial Research Organisation Processo para produzir um produto de óleo e processo para produzir um produto de óleo a partir do óleo da planta
AU2017320470B2 (en) 2016-09-02 2023-11-23 Nuseed Global Innovation Ltd Plants with modified traits

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4943674A (en) 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US5753475A (en) 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
US4795855A (en) 1985-11-14 1989-01-03 Joanne Fillatti Transformation and foreign gene expression with woody species
US5750871A (en) 1986-05-29 1998-05-12 Calgene, Inc. Transformation and foreign gene expression in Brassica species
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5187073A (en) 1986-06-30 1993-02-16 The University Of Toledo Process for transforming gramineae and the products thereof
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5639952A (en) 1989-01-05 1997-06-17 Mycogen Plant Science, Inc. Dark and light regulated chlorophyll A/B binding protein promoter-regulatory system
US5086169A (en) 1989-04-20 1992-02-04 The Research Foundation Of State University Of New York Isolated pollen-specific promoter of corn
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
JP3234598B2 (ja) 1990-11-23 2001-12-04 プラント・ジエネテイツク・システムズ・エヌ・ベー 単子葉植物の形質転換方法
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
AU669384B2 (en) 1992-07-09 1996-06-06 Pioneer Hi-Bred International, Inc. Maize pollen-specific polygalacturonase gene
HUT70467A (en) 1992-07-27 1995-10-30 Pioneer Hi Bred Int An improved method of agrobactenium-mediated transformation of cultvred soyhean cells
US5545169A (en) 1993-04-01 1996-08-13 Yarger; Richard J. Laparoscopic delivery device
DK77393D0 (da) 1993-06-29 1993-06-29 Novo Nordisk As Aktivering af enzymer
EP0731632B1 (en) 1993-12-09 2001-11-07 THE TEXAS A&M UNIVERSITY SYSTEM Agrobacterium tumefaciens transformation of musa species
GB9421286D0 (en) 1994-10-21 1994-12-07 Danisco Promoter
US5536653A (en) 1994-11-04 1996-07-16 Monsanto Company Tomato fruit promoters
US5846797A (en) 1995-10-04 1998-12-08 Calgene, Inc. Cotton transformation
GB9606062D0 (en) 1996-03-22 1996-05-22 Zeneca Ltd Promoters
US6127179A (en) 1996-04-17 2000-10-03 Dellapenna; Dean Gene promoter for tomato fruit
DE19644478A1 (de) 1996-10-25 1998-04-30 Basf Ag Blattspezifische Expression von Genen in transgenen Pflanzen
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US5952543A (en) 1997-02-25 1999-09-14 Dna Plant Technology Corporation Genetically transformed pineapple plants and methods for their production
US5968830A (en) 1997-03-28 1999-10-19 Mississippi State University Soybean transformation and regeneration methods
JP2002504819A (ja) 1997-06-05 2002-02-12 カルジーン エルエルシー ジアシルグリセロールトランスフェラーゼタンパク質
US6037522A (en) 1998-06-23 2000-03-14 Rhone-Poulenc Agro Agrobacterium-mediated transformation of monocots
US6344548B1 (en) 1998-06-24 2002-02-05 The Regents Of The University Of California Diacylglycerol o-acyltransferase
US20030074695A1 (en) * 1998-06-24 2003-04-17 Farese Robert V. Plant diacylglycerol O-acyltransferase and uses thereof
CA2331329C (en) 1998-07-02 2011-08-30 Calgene Llc Diacylglycerol acyl transferase proteins
US7135617B2 (en) 1998-07-02 2006-11-14 Calgene Llc Diacylglycerol acyl transferase proteins
US6100077A (en) 1998-10-01 2000-08-08 The Trustees Of Columbia University In The City Of New York Isolation of a gene encoding diacylglycerol acyltransferase
EP1135473A2 (en) * 1998-12-02 2001-09-26 E.I. Dupont De Nemours And Company Sequenzes of a putative plant diacylglycerol acyltransferases
US6342657B1 (en) 1999-05-06 2002-01-29 Rhone-Poulenc Agro Seed specific promoters
US20110214206A1 (en) * 1999-05-06 2011-09-01 La Rosa Thomas J Nucleic acid molecules and other molecules associated with plants
US7560542B2 (en) * 1999-05-07 2009-07-14 Monsanto Technology Llc Nucleic acid molecule SEQ ID NO. 68811 and other molecules associated with plants
US7642346B2 (en) 1999-08-27 2010-01-05 Sembiosys Genetics Inc. Flax seed specific promoters
CA2390819A1 (en) 2000-01-21 2001-07-26 Pioneer Hi-Bred International, Inc. Novel root-preferred promoter elements and methods of use
US6552250B1 (en) * 2000-06-14 2003-04-22 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Diacylglycerol O-acyltransferase
AU2001291656A1 (en) 2000-06-30 2002-01-08 Willem Broekaert Gene silencing vector
CA2430642A1 (en) 2000-12-01 2003-02-20 John B. Ohlrogge Plant seed specific promoters
US20040067506A1 (en) 2000-12-04 2004-04-08 Ben Scheres Novel root specific promoter driving the expression of a novel lrr receptor-like kinase
EP1256629A1 (en) 2001-05-11 2002-11-13 Société des Produits Nestlé S.A. Leaf specifc gene promoter of coffee
EP1543130A4 (en) 2002-07-31 2006-07-12 Monsanto Technology Llc DIACYLGLYCERIN ACYL TRANSFERASE NUCLEIC ACID SEQUENCES AND ASSOCIATED PRODUCTS
US7371928B2 (en) 2002-11-11 2008-05-13 Korea Chungang Educational Foundation Plant seed-specific expression promoter derived from sesame and seed-specific expression vector comprising the promoter
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
EP1528104A1 (en) 2003-11-03 2005-05-04 Biogemma MEG1 endosperm-specific promoters and genes
WO2005100575A2 (en) 2004-04-14 2005-10-27 Bayer Bioscience N.V. Rice pollen-specific promoters and uses thereof
US7273746B2 (en) 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7588931B2 (en) * 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
MY187548A (en) 2005-06-13 2021-09-29 Government Of Malaysia As Represented By The Mini Of Science Tehnology And Innovation Malaysia Leaf-specific chlorophyll a/b binding protein promoter
MX2008010989A (es) * 2006-03-01 2009-01-26 Pionner Hi Bred International Composiciones relacionadas con el locus 6 de rasgo cuantitativo (qtl6) en maiz y metodos de uso.
US8299318B2 (en) 2007-07-05 2012-10-30 Ceres, Inc. Nucleotide sequences and corresponding polypeptides conferring modulated plant characteristics
US8362325B2 (en) 2007-10-03 2013-01-29 Ceres, Inc. Nucleotide sequences and corresponding polypeptides conferring modulated plant characteristics
CA2990650C (en) * 2008-05-23 2021-02-09 Pioneer Hi-Bred International, Inc. Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
CA2778150C (en) 2009-10-30 2022-07-26 Agresearch Limited Modified neutral lipid encapsulating proteins and uses thereof
US8987551B2 (en) * 2009-10-30 2015-03-24 Agresearch Limited Modified oil encapsulating proteins and uses thereof
PL2914726T3 (pl) 2012-10-30 2019-06-28 Agresearch Limited Udoskonalone polinukleotydy acylotransferazy, polipeptydy i sposoby stosowania
IN2015DN04034A (es) 2012-10-30 2015-10-02 Agres Ltd

Also Published As

Publication number Publication date
EP2914725A2 (en) 2015-09-09
WO2014068438A2 (en) 2014-05-08
IN2015DN03967A (es) 2015-10-02
AU2013340444A1 (en) 2015-05-07
MX359167B (es) 2018-09-18
MX2015005270A (es) 2015-07-17
US9957519B2 (en) 2018-05-01
AU2013340444B2 (en) 2019-08-29
BR112015009303A2 (pt) 2017-08-22
CA2889980A1 (en) 2014-05-08
CN105121646B (zh) 2020-07-21
US20150252378A1 (en) 2015-09-10
EP2914725A4 (en) 2016-04-20
WO2014068438A3 (en) 2014-07-24
CN105121646A (zh) 2015-12-02
NZ706829A (en) 2019-04-26

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