IN2015DN03967A - - Google Patents

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Publication number
IN2015DN03967A
IN2015DN03967A IN3967DEN2015A IN2015DN03967A IN 2015DN03967 A IN2015DN03967 A IN 2015DN03967A IN 3967DEN2015 A IN3967DEN2015 A IN 3967DEN2015A IN 2015DN03967 A IN2015DN03967 A IN 2015DN03967A
Authority
IN
India
Prior art keywords
dgat1
novel
cells
polynucleotides
protein
Prior art date
Application number
Inventor
Nicholas John Roberts
Amy Christina Curran
Somrutai Winichayakul
Marissa Roldan
Richard William Scott
Original Assignee
Agres Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agres Ltd filed Critical Agres Ltd
Publication of IN2015DN03967A publication Critical patent/IN2015DN03967A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides a novel DGAT1 protein with improved properties over known DGAT proteins particularly known DGAT1 proteins from plants. The novel DGAT1 protein of the invention can be expressed in cells to increase cellular lipid accumulation. Expression of the DGAT1 protein of the invention in cells results in a higher level of lipid than any of several other plant DGAT1 proteins tested by the applicants. The invention provides polynucleotides encoding the novel DGAT1 protein of SEQ ID NO:39 constructs cells plant plant parts and progeny comprising the polynucleotides and methods of use of the polynucleotides and polypeptides of the invention.
IN3967DEN2015 2012-10-30 2013-10-22 IN2015DN03967A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261720136P 2012-10-30 2012-10-30
PCT/IB2013/059525 WO2014068438A2 (en) 2012-10-30 2013-10-22 Novel acyltransferase polynucleotides, polypeptides, and methods of use

Publications (1)

Publication Number Publication Date
IN2015DN03967A true IN2015DN03967A (en) 2015-10-02

Family

ID=50628189

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3967DEN2015 IN2015DN03967A (en) 2012-10-30 2013-10-22

Country Status (11)

Country Link
US (1) US9957519B2 (en)
EP (1) EP2914725A4 (en)
CN (1) CN105121646B (en)
AR (1) AR093293A1 (en)
AU (1) AU2013340444B2 (en)
BR (1) BR112015009303A2 (en)
CA (1) CA2889980A1 (en)
IN (1) IN2015DN03967A (en)
MX (1) MX359167B (en)
NZ (1) NZ706829A (en)
WO (1) WO2014068438A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105473718A (en) * 2012-10-30 2016-04-06 农业研究有限公司 Enhanced acyltransferase polynucleotides, polypeptides, and methods of use
WO2014068439A2 (en) 2012-10-30 2014-05-08 Agresearch Limited Improved acyltransferase polynucleotides, polypeptides, and methods of use
AU2013365731B2 (en) 2012-12-21 2018-11-22 The New Zealand Institute For Plant And Food Research Limited Regulation of gene expression
JP6882160B2 (en) 2014-07-07 2021-06-02 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション The process of manufacturing industrial products from plant lipids
BR112019004530A2 (en) 2016-09-02 2019-10-22 Commw Scient Ind Res Org plants with modified traits

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CN105473718A (en) 2012-10-30 2016-04-06 农业研究有限公司 Enhanced acyltransferase polynucleotides, polypeptides, and methods of use

Also Published As

Publication number Publication date
MX2015005270A (en) 2015-07-17
CN105121646B (en) 2020-07-21
CN105121646A (en) 2015-12-02
US20150252378A1 (en) 2015-09-10
AU2013340444B2 (en) 2019-08-29
CA2889980A1 (en) 2014-05-08
EP2914725A4 (en) 2016-04-20
US9957519B2 (en) 2018-05-01
EP2914725A2 (en) 2015-09-09
WO2014068438A2 (en) 2014-05-08
BR112015009303A2 (en) 2017-08-22
AR093293A1 (en) 2015-05-27
NZ706829A (en) 2019-04-26
WO2014068438A3 (en) 2014-07-24
MX359167B (en) 2018-09-18
AU2013340444A1 (en) 2015-05-07

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