AR092113A1 - METODO DE USAR a-AMILASA DE ASPERGILLUS CLAVATUS E ISOAMILASA PARA LA SACARIFICACION - Google Patents

METODO DE USAR a-AMILASA DE ASPERGILLUS CLAVATUS E ISOAMILASA PARA LA SACARIFICACION

Info

Publication number
AR092113A1
AR092113A1 ARP130102869A ARP130102869A AR092113A1 AR 092113 A1 AR092113 A1 AR 092113A1 AR P130102869 A ARP130102869 A AR P130102869A AR P130102869 A ARP130102869 A AR P130102869A AR 092113 A1 AR092113 A1 AR 092113A1
Authority
AR
Argentina
Prior art keywords
amylase
saccharification
composition
acamy1
isoamylase
Prior art date
Application number
ARP130102869A
Other languages
English (en)
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of AR092113A1 publication Critical patent/AR092113A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385—Concentrates of non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/246—Isoamylase (3.2.1.68)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01068—Isoamylase (3.2.1.68)
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/40—Specific cleaning or washing processes
    • C11D2111/44—Multi-step processes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Emergency Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Detergent Compositions (AREA)
  • Fodder In General (AREA)
  • Washing And Drying Of Tableware (AREA)
  • Accessory Of Washing/Drying Machine, Commercial Washing/Drying Machine, Other Washing/Drying Machine (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

Se proporciona a-amilasa fúngica de Aspergillus clavatus (AcAmy1). La AcAmy1 tiene un pH óptimo de 4,5 y puede operar a 30 - 75ºC, lo que permite usar la enzima en combinación con una glucoamilasa y una isoamilasa en una reacción de sacarificación. Esto obvia la necesidad de realizar una reacción de sacarificación en forma de un proceso discontinuo, donde se deben reajustar el pH y la temperatura para el uso óptimo de la a-amilasa o glucoamilasa. La AcAmy1 también cataliza la sacarificación de los sustratos de almidón a una composición de oligosacárico enriquecida significativamente en DP2 y (DP1 + DP2) en comparación con los productos de la sacarificación catalizados por una a-amilasa de Aspergillus kawachii. Esto facilita, por ejemplo, la utilización de la composición de oligosacáridos por parte de un organismo fermentador en un proceso de sacarificación y fermentación simultáneas. Método para producir una composición alimenticia. Composición para eliminar manchas de almidón.
ARP130102869A 2012-08-16 2013-08-13 METODO DE USAR a-AMILASA DE ASPERGILLUS CLAVATUS E ISOAMILASA PARA LA SACARIFICACION AR092113A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261683965P 2012-08-16 2012-08-16

Publications (1)

Publication Number Publication Date
AR092113A1 true AR092113A1 (es) 2015-03-25

Family

ID=48986283

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130102869A AR092113A1 (es) 2012-08-16 2013-08-13 METODO DE USAR a-AMILASA DE ASPERGILLUS CLAVATUS E ISOAMILASA PARA LA SACARIFICACION

Country Status (9)

Country Link
US (1) US20150232901A1 (es)
EP (1) EP2885417A1 (es)
JP (1) JP2015534456A (es)
CN (1) CN104640994A (es)
AR (1) AR092113A1 (es)
BR (1) BR112015003071A2 (es)
CA (1) CA2878616A1 (es)
MX (1) MX2015001823A (es)
WO (1) WO2014028436A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2878988A1 (en) * 2012-08-16 2014-02-20 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
WO2014099416A1 (en) * 2012-12-20 2014-06-26 Danisco Us Inc. Method of using alpha-amylase from aspergillus terreus and isoamylase for saccharification
EP3310910A1 (en) * 2015-06-18 2018-04-25 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
BR112018012643A2 (pt) * 2015-12-21 2018-12-04 C Koops Bart enzimas e métodos de conversão de amido granular aprimorada
WO2017112635A1 (en) * 2015-12-21 2017-06-29 Danisco Us Inc Improved granular starch conversion enzymes and methods
EP3272767B1 (en) * 2016-07-21 2020-11-25 Fornia BioSolutions, Inc. G24 glucoamylase compositions and methods
WO2018017105A1 (en) * 2016-07-21 2018-01-25 Fornia Biosolutions, Inc. G24 glucoamylase compositions and methods
JP7008453B2 (ja) * 2016-12-26 2022-01-25 キリンホールディングス株式会社 ビールテイスト発酵アルコール飲料およびその製造方法
JP7115830B2 (ja) * 2017-09-29 2022-08-09 群栄化学工業株式会社 麦汁もしくは麦芽エキス、又は醸造酒の製造方法
CN111220604B (zh) * 2019-12-06 2022-03-15 东北农业大学 一种肉制品中总淀粉含量的测定方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5162210A (en) * 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
JPH05236958A (ja) * 1992-02-28 1993-09-17 Amano Pharmaceut Co Ltd 新規イソアミラーゼ及びその製造法
AU1434299A (en) * 1997-11-26 1999-06-16 Novo Nordisk A/S Thermostable glucoamylase
KR100764528B1 (ko) * 1998-07-15 2007-10-09 노보자임스 에이/에스 글루코아밀라제 변이체
US20060075522A1 (en) * 2004-07-31 2006-04-06 Jaclyn Cleveland Genes and uses for plant improvement
CN101128580B (zh) * 2004-12-22 2016-08-24 诺维信公司 用于淀粉加工的酶
AU2005319074B2 (en) * 2004-12-22 2011-03-24 Novozymes A/S Enzymes for starch processing
BRPI0808513A2 (pt) * 2007-03-09 2014-08-19 Danisco Us Inc Genencor Div Variantes de alfa-amilase de espécies de bacillus alcalifílico, composições compreendendo variantes de alfa-amilase e métodos de uso
CA2689629A1 (en) * 2007-05-30 2008-12-18 Danisco Us Inc. Improved variants of the bacillus licheniformis alpha-amylase
DK2222842T3 (en) * 2007-11-20 2015-01-19 Danisco Us Inc Glucoamylasevarianter with changed properties
US20090205075A1 (en) * 2008-01-30 2009-08-13 Stacy Miles Use of plastid transit peptides derived from glaucocystophytes
MX349630B (es) * 2010-01-04 2017-08-07 Novozymes As Alfa-amilasas.

Also Published As

Publication number Publication date
MX2015001823A (es) 2015-05-07
WO2014028436A1 (en) 2014-02-20
EP2885417A1 (en) 2015-06-24
CN104640994A (zh) 2015-05-20
CA2878616A1 (en) 2014-02-20
US20150232901A1 (en) 2015-08-20
BR112015003071A2 (pt) 2019-09-24
JP2015534456A (ja) 2015-12-03

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