AR046598A1 - Vectores retrovirales para la administracion de arn de interferencia - Google Patents
Vectores retrovirales para la administracion de arn de interferenciaInfo
- Publication number
- AR046598A1 AR046598A1 ARP040103824A ARP040103824A AR046598A1 AR 046598 A1 AR046598 A1 AR 046598A1 AR P040103824 A ARP040103824 A AR P040103824A AR P040103824 A ARP040103824 A AR P040103824A AR 046598 A1 AR046598 A1 AR 046598A1
- Authority
- AR
- Argentina
- Prior art keywords
- target gene
- retroviral vector
- double stranded
- stranded rna
- antisense
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/867—Retroviral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se proporcionan vectores retrovirales para administrar ARN de interferencia en células así como células infectadas por dichos vectores, las cuales tienen un oncogen como gen objetivo en su genoma. Reivindicación 1: Un vector retroviral para llevar un insecto especifico del gen objetivo en una célula a fin de modificar la expresión de un gen objetivo, que comprende: (a) un promotor; (b) una región poliligante; (c) un insecto especifico del gen objetivo que comprende ARN de doble hebra, en donde dicho ARN de doble hebra comprende una porción sentido que es complementaria a una porción de la hebra antisentido del gen objetivo, y una porción antisentido que es complementaria a la porción sentido, de modo que la porción sentido y la porción antisentido se hibridan, y el ARN de doble hebra se pliega para atrás sobre si mismo. Reivindicación 8: El vector retroviral de acuerdo con la Reivindicación 1, en el cual el vector retroviral es un virus de célula pluripotente murino (MSCV, abreviatura del ingles "Murine Stem Cell Virus"). Reivindicación 9: El vector retroviral de acuerdo con la Reivindicación 1, en el cual el vector retroviral es un Lentivirus modificado en el cual: (a) el promotor de CMV endógeno del Lentivirus ha sido eliminado; y (b) un elemento REV que se une a un elemento de respuesta REV (RRE, abreviatura del ingles "REV response element") es insertado.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US51331303P | 2003-10-22 | 2003-10-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR046598A1 true AR046598A1 (es) | 2005-12-14 |
Family
ID=34549264
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP040103824A AR046598A1 (es) | 2003-10-22 | 2004-10-21 | Vectores retrovirales para la administracion de arn de interferencia |
Country Status (13)
Country | Link |
---|---|
US (2) | US7612195B2 (es) |
EP (1) | EP1678312A1 (es) |
JP (1) | JP2007508842A (es) |
KR (1) | KR20060107519A (es) |
CN (1) | CN1871356B (es) |
AR (1) | AR046598A1 (es) |
AU (2) | AU2004286231B2 (es) |
BR (1) | BRPI0415646A (es) |
CA (1) | CA2542871C (es) |
IL (1) | IL174804A0 (es) |
MX (1) | MXPA06004428A (es) |
TW (2) | TWI350311B (es) |
WO (1) | WO2005042754A1 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006082385A1 (en) * | 2005-02-01 | 2006-08-10 | Medical Research Council | Screening method |
US20090217397A1 (en) * | 2006-06-12 | 2009-08-27 | Patrick Stern | Cre-lox based gene knockdown constructs and methods of use thereof |
US9043994B2 (en) | 2007-03-13 | 2015-06-02 | Massachusetts Institute Of Technology | Cre-lox based gene knockdown constructs and methods of use thereof |
EP2329024A4 (en) * | 2008-08-25 | 2012-02-22 | Inst Basic Med Sciences Pla | ENDOGENOUS SHORT HAIR NADEL RNA AND ITS USE |
US9481895B2 (en) | 2011-09-23 | 2016-11-01 | Ge Healthcare Dharmacon, Inc. | Introduction of modular vector elements during production of a lentivirus |
CN103060374B (zh) * | 2012-10-30 | 2014-05-07 | 中国农业科学院兰州兽医研究所 | 一种基于位点特异性重组的rna干扰载体及其构建方法和应用 |
CN105586360A (zh) * | 2016-03-04 | 2016-05-18 | 武汉科技大学 | 一种同时表达小分子rna和蛋白质的双功能慢病毒表达载体及其制备方法和应用 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5624803A (en) * | 1993-10-14 | 1997-04-29 | The Regents Of The University Of California | In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom |
US6624803B1 (en) * | 1995-10-20 | 2003-09-23 | Wisconsin Alumni Research Foundation | Interface for electronic devices providing improved access for people with disabilities |
CN1429911A (zh) * | 2001-12-30 | 2003-07-16 | 李卫云 | 慢病毒载体系统,产生载体的方法及其应用 |
US20040248296A1 (en) * | 2002-03-20 | 2004-12-09 | Beresford Paul J. | HIV therapeutic |
AU2003268546A1 (en) * | 2002-09-06 | 2004-03-29 | Massachusetts Institute Of Technology | Lentiviral vectors, related reagents, and methods of use thereof |
AU2003297474A1 (en) * | 2002-12-18 | 2004-07-14 | Salk Institute For Biological Studies | Methods of inhibiting gene expression by rna interference |
-
2004
- 2004-10-21 US US10/574,416 patent/US7612195B2/en not_active Expired - Fee Related
- 2004-10-21 EP EP04796001A patent/EP1678312A1/en not_active Withdrawn
- 2004-10-21 MX MXPA06004428A patent/MXPA06004428A/es active IP Right Grant
- 2004-10-21 CA CA2542871A patent/CA2542871C/en not_active Expired - Fee Related
- 2004-10-21 AR ARP040103824A patent/AR046598A1/es unknown
- 2004-10-21 KR KR1020067007790A patent/KR20060107519A/ko active IP Right Grant
- 2004-10-21 AU AU2004286231A patent/AU2004286231B2/en not_active Ceased
- 2004-10-21 JP JP2006536797A patent/JP2007508842A/ja active Pending
- 2004-10-21 CN CN2004800313354A patent/CN1871356B/zh not_active Expired - Fee Related
- 2004-10-21 WO PCT/US2004/034932 patent/WO2005042754A1/en active Application Filing
- 2004-10-21 BR BRPI0415646-3A patent/BRPI0415646A/pt not_active Application Discontinuation
- 2004-10-22 TW TW093132069A patent/TWI350311B/zh not_active IP Right Cessation
- 2004-10-22 TW TW099140518A patent/TW201111508A/zh unknown
-
2006
- 2006-04-05 IL IL174804A patent/IL174804A0/en unknown
-
2009
- 2009-10-07 US US12/575,005 patent/US7884200B2/en not_active Expired - Fee Related
- 2009-12-09 AU AU2009248456A patent/AU2009248456B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2542871A1 (en) | 2005-05-12 |
US7884200B2 (en) | 2011-02-08 |
KR20060107519A (ko) | 2006-10-13 |
AU2009248456B2 (en) | 2011-09-15 |
TWI350311B (en) | 2011-10-11 |
US20060252153A1 (en) | 2006-11-09 |
AU2004286231A1 (en) | 2005-05-12 |
AU2004286231B2 (en) | 2009-09-10 |
BRPI0415646A (pt) | 2006-12-12 |
TW201111508A (en) | 2011-04-01 |
US7612195B2 (en) | 2009-11-03 |
WO2005042754A1 (en) | 2005-05-12 |
CA2542871C (en) | 2010-11-16 |
AU2009248456A1 (en) | 2010-01-07 |
IL174804A0 (en) | 2006-08-20 |
EP1678312A1 (en) | 2006-07-12 |
CN1871356B (zh) | 2011-04-27 |
CN1871356A (zh) | 2006-11-29 |
TW200523362A (en) | 2005-07-16 |
JP2007508842A (ja) | 2007-04-12 |
MXPA06004428A (es) | 2006-07-03 |
US20100028996A1 (en) | 2010-02-04 |
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