AR006016A1 - Metodo para determinar h.pylori en muestras fecales y un equipo (kit) para realizar dicho metodo - Google Patents
Metodo para determinar h.pylori en muestras fecales y un equipo (kit) para realizar dicho metodoInfo
- Publication number
- AR006016A1 AR006016A1 ARP970100781A ARP970100781A AR006016A1 AR 006016 A1 AR006016 A1 AR 006016A1 AR P970100781 A ARP970100781 A AR P970100781A AR P970100781 A ARP970100781 A AR P970100781A AR 006016 A1 AR006016 A1 AR 006016A1
- Authority
- AR
- Argentina
- Prior art keywords
- pylori
- complex
- antibody
- kit
- antigen
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56922—Campylobacter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/205—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Campylobacter (G)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se trata de un proceso para determinar H.piloris muestras fecales, que comprende: (a) dispersar la muestra fecal en un diluyente; (b) poner encontacto la muestra fecal en el diluyente, con un primer anticuerpo policlonal para el antígeno deH.p ilori, para formar un complejo del anticuerpo y delantígeno; (c) separar dicha muestra y dicho complejo; (d) exponer el complejo a un segundo anticuerpo policlonal para dicho antígeno y una porción delanticuerpo que reacciona con dicho complejo,esta ndo uno de dichos anticuerpos primero y segundo, vinculado a un vehículo sólido, y estando el otro, marcadacon un agente de detección; y (e)determinar la cantidad del anticuerpo etiquetado y a su vez determinar la presencia delantígeno del H. pilori endicha muestra fecal. También se propone un kit para detectar H. pilori mediante inmunoensayos aplicado sobre muestras fecales.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/647,115 US5716791A (en) | 1996-05-09 | 1996-05-09 | Immunoassay for H. pylori in fecal specimens |
Publications (1)
Publication Number | Publication Date |
---|---|
AR006016A1 true AR006016A1 (es) | 1999-07-21 |
Family
ID=24595765
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP970100781A AR006016A1 (es) | 1996-05-09 | 1997-02-27 | Metodo para determinar h.pylori en muestras fecales y un equipo (kit) para realizar dicho metodo |
Country Status (12)
Country | Link |
---|---|
US (3) | US5716791A (es) |
EP (1) | EP0806667B2 (es) |
JP (1) | JP3043999B2 (es) |
CN (1) | CN1271408C (es) |
AR (1) | AR006016A1 (es) |
AT (1) | ATE211552T1 (es) |
AU (1) | AU698513B2 (es) |
CA (1) | CA2198336C (es) |
DE (2) | DE806667T1 (es) |
ES (1) | ES2132044T5 (es) |
GR (1) | GR990300022T1 (es) |
MX (1) | MX9701877A (es) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5716791A (en) * | 1996-05-09 | 1998-02-10 | Meridian Diagnostics, Inc. | Immunoassay for H. pylori in fecal specimens |
CA2224551A1 (en) * | 1998-02-25 | 1999-08-25 | Alex Hongsheng Chang | A quick in situ diagnostic method for detecting alimentary diseases |
DE69939939D1 (de) | 1998-08-11 | 2009-01-02 | Idec Pharma Corp | Kombinationstherapien gegen b-zell-lymphome beinhaltend die verabreichung von anti-cd20-antikörpern |
JP2002529705A (ja) * | 1998-10-29 | 2002-09-10 | コンネクス・ゲーエムベーハー | 糞便中の耐酸性微生物を検出するための新規方法 |
US8557244B1 (en) | 1999-08-11 | 2013-10-15 | Biogen Idec Inc. | Treatment of aggressive non-Hodgkins lymphoma with anti-CD20 antibody |
DK1232392T3 (da) | 1999-10-12 | 2003-07-28 | Connex Ges Zur Optimierung Von | Forbedret fremgangsmåde til påvisning af syreresistente bakterier af slægten Helicobacter i afføring |
JP3504633B2 (ja) * | 1999-10-29 | 2004-03-08 | わかもと製薬株式会社 | ヘリコバクター・ピロリへの感染を判定する検査方法及び検査試薬 |
EP1227159B1 (en) | 1999-10-29 | 2011-04-20 | Wakamoto Pharmaceutical Co., Ltd. | Monoclonal antibody, hybridoma, immunoassay method and diagnosis kit |
WO2001040801A2 (en) * | 1999-12-03 | 2001-06-07 | Meridian Bioscience, Inc. | Diagnosis and treatment for helicobacter pylori induced colic |
TWI237695B (en) * | 1999-12-14 | 2005-08-11 | Joy Biomedical Corp | Helicobacter pylori antigens in blood |
DE10006432A1 (de) * | 2000-02-14 | 2001-08-16 | Ganzimmun Inst Fuer Ganzheitli | Verfahren zum Nachweis von Helicobacter pylori in Stuhl- und Speichelproben |
EP1156331B8 (en) * | 2000-05-18 | 2009-08-19 | Meridian Bioscience, Inc. | Immunoassay for H. Pylori in fecal specimens using genus specific monoclonal antibody |
WO2002014541A1 (en) * | 2000-08-11 | 2002-02-21 | Wakamoto Pharmaceutical Co., Ltd. | Method of examining infection with helicobacter pylori and diagnostic kit |
JP4443117B2 (ja) * | 2001-04-23 | 2010-03-31 | わかもと製薬株式会社 | イムノクロマトグラフィー試験片及び診断キット |
JP4763149B2 (ja) * | 2001-05-10 | 2011-08-31 | わかもと製薬株式会社 | ヘリコバクター・ピロリへの感染を判定する検査方法 |
FI118061B (fi) | 2001-09-24 | 2007-06-15 | Beanor Oy | Menetelmä ja bioanturi analyysiä varten |
FI115166B (fi) | 2001-12-31 | 2005-03-15 | Biofons Oy | Diagnostisia menetelmiä |
DE10219741A1 (de) * | 2002-05-02 | 2003-11-13 | Georg S Wengler | Verfahren zur Vorbehandlung von Stuhlproben |
KR20040046427A (ko) * | 2002-11-27 | 2004-06-05 | 주식회사 비엘에스 | 분변 시료 중의 헬리코박터 피로리의 검출방법 |
WO2009005536A2 (en) * | 2006-11-22 | 2009-01-08 | 3M Innovative Properties Company | Methods of capturing bacterial whole cells and methods of analyzing samples for bacteria |
US8025880B2 (en) * | 2007-03-01 | 2011-09-27 | Helicure Ab | Immunoglobulin against Helicobacter pylori |
US20090263905A1 (en) * | 2008-04-18 | 2009-10-22 | Kim Scheuringer | Detection test assembly for detecting the presence of a substance in a sample |
US9110053B2 (en) * | 2010-08-20 | 2015-08-18 | Agilent Technologies, Inc. | Dried blood spotting paper device and method |
US8541180B1 (en) | 2010-10-11 | 2013-09-24 | Genova Diagnostics, Inc. | Compositions and methods for assessing gastrointestinal health |
CN102565391A (zh) * | 2011-12-23 | 2012-07-11 | 王滔 | 胃黏膜样品中幽门螺杆菌的免疫测定法 |
CN103293305A (zh) * | 2012-03-05 | 2013-09-11 | 美利泰格诊断试剂(嘉兴)有限公司 | 口腔螺旋杆菌感染检测方法及用于检测的唾液试板 |
CN102980884A (zh) * | 2012-11-29 | 2013-03-20 | 江苏创生生物技术有限公司 | 胃黏膜样品中幽门螺旋杆菌的化学发光免疫测定法 |
CN103149067A (zh) * | 2013-02-28 | 2013-06-12 | 苏州和锐医药科技有限公司 | 一种粪便蛋白萃取方法及试剂 |
KR101619862B1 (ko) * | 2014-09-30 | 2016-05-12 | 주식회사 녹십자 | 간접효소면역측정법을 이용하는 인간 Fc 포함 단백질의 역가 측정키트 및 이를 이용한 Fc 포함 단백질의 역가 측정방법 |
DE102015000626A1 (de) * | 2015-01-22 | 2016-07-28 | Kibion Gmbh | Verfahren zum Nachweis von Helicobacter Pylori |
CN109342722A (zh) * | 2018-09-30 | 2019-02-15 | 深圳市鸿美诊断技术有限公司 | 一种用于定量测定粪便中幽门螺杆菌抗原试剂的制备方法 |
Family Cites Families (21)
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AR231590A1 (es) * | 1981-04-29 | 1984-12-28 | Ciba Geigy Ag | Dispositivo de analisis inmunologico y procedimiento para obtenerlo |
DE3785590T2 (de) * | 1986-01-22 | 1993-09-16 | Amoco Corp | Campylobacter-sonde. |
US4942126A (en) * | 1986-08-13 | 1990-07-17 | Allegheny-Singer Research Institute | Method of extracting antigens of bacteria |
EP0281251A3 (en) * | 1987-02-04 | 1988-09-28 | International Immunoassay Laboratories, Inc. | A method for preparing a fecal sample composition for immunoassay testing |
US4923801A (en) * | 1987-04-13 | 1990-05-08 | The University Of Virginia Alumni Patents Foundation | Compositions and methods for the enrichment and isolation of Campylobacter pylori and related organisms from biological specimens and the environment |
US5459041A (en) * | 1988-02-18 | 1995-10-17 | Enteric Research Laboratories, Inc. | Campylobacter pylori antigens and uses thereof for detection of Campylobacter pylori infection |
US4882271A (en) * | 1988-03-10 | 1989-11-21 | Baylor College Of Medicine | Process for preparation of high molecular weight cell-associated protein of campylobacter pylori and use for serological detection of campylobacter pylori infection |
US5017342A (en) * | 1988-06-01 | 1991-05-21 | Ortho Diagnostic Systems Inc. | Device for immunoassay determinations |
US5571674A (en) * | 1989-09-18 | 1996-11-05 | The Trustees Of Columbia University In The City Of New York | DNA oligomers for use in detection of campylobacter pylori and methods of using such DNA oligomers |
AU644121B2 (en) * | 1989-12-04 | 1993-12-02 | Auspharm International Limited | Rapid in vitro test for helicobacter pylori using saliva |
JP2988635B2 (ja) * | 1990-09-18 | 1999-12-13 | 塩野義製薬株式会社 | ヒトIgEに対するモノクローナル抗体 |
US5262156A (en) * | 1991-08-12 | 1993-11-16 | Hycor Biomedical, Inc. | Antigenic compositions and their use for the detection of Helicobacter pylori |
DK0638175T3 (da) * | 1992-04-29 | 1999-04-06 | Auspharm Int Ltd | In vitro-test for Helicobacter pylori |
US5420014A (en) * | 1992-04-30 | 1995-05-30 | Auspharm International Ltd. | Rapid in vitro test for helicobacter pylori using saliva |
US5403924A (en) * | 1992-10-13 | 1995-04-04 | Vanderbilt University | Taga gene and methods for detecting predisposition to peptic ulceration |
EP0666987A4 (en) * | 1992-10-30 | 1997-08-27 | T Cell Diagnostics Inc | EVALUATION OF THE TOTAL QUANTITY OF A MOLECULE IN A SAMPLE AND METHODS BASED ON SAME. |
GB9313437D0 (en) † | 1993-06-30 | 1993-08-11 | Smith Andrew W | Pathogenicity sequences in helicobacter pylori |
US5486361A (en) * | 1993-10-25 | 1996-01-23 | The United States Of America As Represented By The Department Of Health And Human Services | Hybridomas and monoclonal antibodies that speifically bind to GPIB on platelets and inhibit the binding of thrombin to platelets |
US5498528A (en) * | 1994-06-10 | 1996-03-12 | King; Wing | Detection of helicobacter pylori |
US5610060A (en) * | 1994-06-24 | 1997-03-11 | The United States Of America As Represented By The Department Of Health And Human Services | Isolated Helicobacter hepaticus |
US5716791A (en) * | 1996-05-09 | 1998-02-10 | Meridian Diagnostics, Inc. | Immunoassay for H. pylori in fecal specimens |
-
1996
- 1996-05-09 US US08/647,115 patent/US5716791A/en not_active Ceased
-
1997
- 1997-02-24 CA CA002198336A patent/CA2198336C/en not_active Expired - Lifetime
- 1997-02-27 AR ARP970100781A patent/AR006016A1/es active IP Right Grant
- 1997-02-28 AU AU15029/97A patent/AU698513B2/en not_active Expired
- 1997-03-12 MX MX9701877A patent/MX9701877A/es unknown
- 1997-03-12 CN CN97103112.6A patent/CN1271408C/zh not_active Expired - Lifetime
- 1997-03-17 JP JP9062637A patent/JP3043999B2/ja not_active Expired - Fee Related
- 1997-05-08 EP EP97303147A patent/EP0806667B2/en not_active Expired - Lifetime
- 1997-05-08 DE DE0806667T patent/DE806667T1/de active Pending
- 1997-05-08 DE DE69709434T patent/DE69709434T3/de not_active Expired - Lifetime
- 1997-05-08 AT AT97303147T patent/ATE211552T1/de not_active IP Right Cessation
- 1997-05-08 ES ES97303147T patent/ES2132044T5/es not_active Expired - Lifetime
- 1997-07-21 US US08/897,732 patent/US5871942A/en not_active Expired - Lifetime
-
1999
- 1999-01-01 GR GR990300022T patent/GR990300022T1/el unknown
-
2000
- 2000-02-08 US US09/500,365 patent/USRE38088E1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
USRE38088E1 (en) | 2003-04-22 |
MX9701877A (es) | 1998-04-30 |
ES2132044T3 (es) | 2002-03-16 |
US5871942A (en) | 1999-02-16 |
DE806667T1 (de) | 1999-10-07 |
JPH1010128A (ja) | 1998-01-16 |
AU698513B2 (en) | 1998-10-29 |
DE69709434T3 (de) | 2009-01-02 |
DE69709434D1 (de) | 2002-02-07 |
EP0806667B1 (en) | 2002-01-02 |
ES2132044T1 (es) | 1999-08-16 |
US5716791A (en) | 1998-02-10 |
EP0806667B2 (en) | 2008-07-09 |
CA2198336C (en) | 2002-06-18 |
CA2198336A1 (en) | 1997-11-10 |
CN1271408C (zh) | 2006-08-23 |
CN1165299A (zh) | 1997-11-19 |
ES2132044T5 (es) | 2008-12-01 |
AU1502997A (en) | 1997-11-13 |
DE69709434T2 (de) | 2002-09-19 |
GR990300022T1 (en) | 1999-07-30 |
EP0806667A1 (en) | 1997-11-12 |
ATE211552T1 (de) | 2002-01-15 |
JP3043999B2 (ja) | 2000-05-22 |
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Legal Events
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