KR910006728A - 분석물의 검출 방법 - Google Patents

분석물의 검출 방법 Download PDF

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Publication number
KR910006728A
KR910006728A KR1019900013798A KR900013798A KR910006728A KR 910006728 A KR910006728 A KR 910006728A KR 1019900013798 A KR1019900013798 A KR 1019900013798A KR 900013798 A KR900013798 A KR 900013798A KR 910006728 A KR910006728 A KR 910006728A
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KR
South Korea
Prior art keywords
analytes
analyte
detection
mixture
reactivity
Prior art date
Application number
KR1019900013798A
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English (en)
Other versions
KR920007203B1 (ko
Inventor
바움가르텐 호르스트
그롤 미하엘
쉬탈 페터
Original Assignee
독토르 다움
뵈링거 만하임 게엠베하
독토르 포우쿠베트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 독토르 다움, 뵈링거 만하임 게엠베하, 독토르 포우쿠베트 filed Critical 독토르 다움
Publication of KR910006728A publication Critical patent/KR910006728A/ko
Application granted granted Critical
Publication of KR920007203B1 publication Critical patent/KR920007203B1/ko

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G01N33/541—Double or second antibody, i.e. precipitating antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding

Abstract

내용 없음.

Description

분석물의 검출 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 두개의 항체 19.6.9 및 1.26.18의 다른 혼합물에 대해 얻은(표준화된) 교차 반응성을 도표로 표시한 것이다.
제2도는 두개의 항체 19.6.9 및 9B4.C2의 다른 혼합물에 대해 얻은(표준화된) 교차 반응성을 도표로 표시한 것이다.
제3도는 두개의 항체 8.15.1 및 700-03의 다른 혼합물에 대해 얻은(표준화된) 교차 반응성을 도표로 표시한 것이다.

Claims (3)

  1. 분석물에 특이적으로 결합할 수 있고 다른 교차 반응성을 갖는 최소한 두개의 단클론 항체의 혼합물이 수용체로서 사용되며, 정량될 분석물이 분석물에 대한 수용체의 결합 부위에 대해 알고 있는 양의 라벨링되거나 고정화된 분석물과 경쟁함에 의한 단클론 분석물의 면역학적 검출방법.
  2. 제1항에 있어서, 0.1 내지 10:1 비율의 두개 단클론 항체의 혼합물이 사용되는 방법.
  3. 제1항 또는 제2항에 있어서, 각각의 테스트에 대한 검출 한계 및 허용가능한 한계의 3배 사이인 하나 또는 여러물질과의 교차 반응성을 갖는 단클론 항체가 사용되는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900013798A 1989-09-01 1990-09-01 분석물의 검출 방법 KR920007203B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3929119A DE3929119A1 (de) 1989-09-01 1989-09-01 Verfahren zum nachweis von analyten
DE3929119.7 1989-09-01

Publications (2)

Publication Number Publication Date
KR910006728A true KR910006728A (ko) 1991-04-29
KR920007203B1 KR920007203B1 (ko) 1992-08-27

Family

ID=6388456

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900013798A KR920007203B1 (ko) 1989-09-01 1990-09-01 분석물의 검출 방법

Country Status (20)

Country Link
US (1) US5312763A (ko)
EP (1) EP0415442B1 (ko)
JP (1) JPH0399266A (ko)
KR (1) KR920007203B1 (ko)
AT (1) ATE111226T1 (ko)
AU (1) AU623276B2 (ko)
CA (1) CA2024175A1 (ko)
CS (1) CS411990A2 (ko)
DD (1) DD297516A5 (ko)
DE (2) DE3929119A1 (ko)
FI (1) FI904304A0 (ko)
HU (1) HUT62091A (ko)
IE (1) IE903110A1 (ko)
IL (1) IL95536A (ko)
NO (1) NO903820L (ko)
NZ (1) NZ235106A (ko)
PT (1) PT95176A (ko)
RU (1) RU1799471C (ko)
YU (1) YU164090A (ko)
ZA (1) ZA906951B (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5616503A (en) * 1992-01-17 1997-04-01 Self; Colin H. Determination of haptens
DE19504198A1 (de) * 1995-02-09 1996-08-14 Behringwerke Ag Kompetitiver Immuntest unter Verwendung komplexierter Analytderivate
CN113125697B (zh) * 2019-12-31 2024-03-26 科美博阳诊断技术(上海)有限公司 一种睾酮均相化学发光检测试剂盒及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56145222A (en) * 1980-04-28 1981-11-11 Toshiyuki Hamaoka Improved antibody and its preparation
CH642458A5 (en) * 1980-04-25 1984-04-13 Hoffmann La Roche Immunological method
NL185309C (nl) * 1980-07-28 1990-03-01 Akzo Nv Methode voor het bepalen van antigenen met behulp van twee of meer monoklonale antilichamen alsmede immuunreagens.
SE8006424L (sv) * 1980-09-12 1982-03-13 Jolla Cancer Res Found Sett att bestemma ett antigen i losning
JPS57208458A (en) * 1981-06-18 1982-12-21 Mitsui Toatsu Chem Inc Labeled antibody for immunochemical measurement
US4514505A (en) * 1982-05-21 1985-04-30 The Trustees Of Columbia University In The City Of New York Monoclonal antibody mixtures and use thereof for enhanced sensitivity immunoassays
US4471058A (en) * 1982-07-26 1984-09-11 Board Of Trustees Operating Michigan State University Method for the detection and/or determination of a polyvalent antigen using at least two different monoclonal antibodies
US4772551A (en) * 1985-12-26 1988-09-20 Neogen Corporation Method and test kit for detecting a trichothecene using novel monoclonal antibodies
AU7202887A (en) * 1986-03-26 1987-10-20 Murex Corp. Device and method for determining microbial resistance to antimicrobic agents
DE3723726A1 (de) * 1987-07-17 1989-02-16 Pfeiffer Bioanalytik Kg Dr Verfahren zur herstellung spezifischer, seitendifferenzierender nachweisreagenzien und ihre anwendung
DE3804244A1 (de) * 1987-12-24 1989-07-06 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer immunologisch aktiven substanz

Also Published As

Publication number Publication date
NO903820D0 (no) 1990-08-31
JPH0399266A (ja) 1991-04-24
US5312763A (en) 1994-05-17
DE3929119A1 (de) 1991-03-07
EP0415442A2 (de) 1991-03-06
EP0415442A3 (en) 1991-07-31
EP0415442B1 (de) 1994-09-07
NZ235106A (en) 1991-11-26
IL95536A0 (en) 1991-06-30
ZA906951B (en) 1991-06-26
CA2024175A1 (en) 1991-03-02
DD297516A5 (de) 1992-01-09
AU6205290A (en) 1991-03-07
NO903820L (no) 1991-03-04
HU905685D0 (en) 1991-03-28
KR920007203B1 (ko) 1992-08-27
YU164090A (sh) 1992-12-21
RU1799471C (ru) 1993-02-28
ATE111226T1 (de) 1994-09-15
AU623276B2 (en) 1992-05-07
IE903110A1 (en) 1991-03-13
CS411990A2 (en) 1991-10-15
DE59007046D1 (de) 1994-10-13
IL95536A (en) 1994-05-30
PT95176A (pt) 1991-05-22
FI904304A0 (fi) 1990-08-31
HUT62091A (en) 1993-03-29

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