ES2066739A1 - Inmunoensayo para analitos en muestras empleando agentes alquilantes. - Google Patents
Inmunoensayo para analitos en muestras empleando agentes alquilantes.Info
- Publication number
- ES2066739A1 ES2066739A1 ES09401086A ES9401086A ES2066739A1 ES 2066739 A1 ES2066739 A1 ES 2066739A1 ES 09401086 A ES09401086 A ES 09401086A ES 9401086 A ES9401086 A ES 9401086A ES 2066739 A1 ES2066739 A1 ES 2066739A1
- Authority
- ES
- Spain
- Prior art keywords
- immunoassay
- analytes
- samples
- alkylating agents
- alkylating agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
Abstract
SE HA DESARROLLADO UN METODO Y UN EQUIPO PARA INMUNOENSAYO PERFECCIONADO, QUE CONSTA DE UN METODO Y EQUIPO DE PRETRATAMIENTO DE LA MUESTRA, EMPLEANDO UN AGENTE ALQUILANTE QUE MODIFICA LOS ANTICUERPOS DE LA MUESTRA PARA CONVERTIR LOS ANTICUERPOS EN INCAPACES DE INTERFERIR CON LA DETECCION DEL ANALITO. ANTES DE EFECTUAR EL INMUNOENSAYO, EL AGENTE ALQUILANTE SE INACTIVA DE FORMA QUE NO MODIFICA LOS ANTICUERPOS REACTIVOS EMPLEADOS EN EL INMUNOENSAYO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/065,019 US5484706A (en) | 1993-05-19 | 1993-05-19 | Immunoassay for analytes in samples using alkylating agents |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2066739A1 true ES2066739A1 (es) | 1995-03-01 |
ES2066739B1 ES2066739B1 (es) | 1995-11-01 |
Family
ID=22059810
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09401086A Expired - Fee Related ES2066739B1 (es) | 1993-05-19 | 1994-05-18 | Inmunoensayo para analitos en muestras empleando agentes alquilantes. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5484706A (es) |
JP (1) | JP3335760B2 (es) |
CN (1) | CN1082663C (es) |
CA (1) | CA2123069C (es) |
DE (1) | DE4416144A1 (es) |
ES (1) | ES2066739B1 (es) |
FR (1) | FR2705463B1 (es) |
GB (1) | GB2278195B (es) |
IT (1) | IT1274006B (es) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9403215D0 (en) * | 1994-02-19 | 1994-04-13 | Kodak Ltd | Chemical modification of antibodies and antigens |
US5478729A (en) * | 1994-04-28 | 1995-12-26 | Syntex (U.S.A.) Inc. | Immunoassay for homocysteine |
US5773234A (en) * | 1995-08-07 | 1998-06-30 | Quidel Corporation | Method and device for chlamydia detection |
US8263415B2 (en) * | 2006-09-14 | 2012-09-11 | Ge Healthcare Bio-Sciences Ab | Method of determining analyte concentration |
EP3470840B1 (en) * | 2014-06-12 | 2021-03-03 | Hyglos Invest GmbH | Unmasking endotoxins in solution |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0148166A2 (en) * | 1984-01-03 | 1985-07-10 | Georgetown University | Breast cancer diagnostic blood test |
US4536478A (en) * | 1984-04-05 | 1985-08-20 | Seragan Diagnostics, Inc. | Method for reducing non-specific interferences in agglutination immunoassays |
US4703001A (en) * | 1985-10-23 | 1987-10-27 | Synbiotics, Corporation | Immunoassay for the detection of serum analytes using pH dependent chastropic acids |
WO1989005975A1 (en) * | 1987-12-16 | 1989-06-29 | Microgenics Corporation | Serum pretreatment in vitamin b12 assay |
EP0599417A2 (en) * | 1992-11-25 | 1994-06-01 | Akzo Nobel N.V. | Method to dissociate an immunocomplex and immunoassay using it |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2047889A (en) * | 1979-04-02 | 1980-12-03 | Research Corp | Detection of antibodies to chlormydia trachomatis having an immunofluorescence test |
US4276050A (en) * | 1980-01-10 | 1981-06-30 | Abbott Laboratories | Method for systemic endotoxin detection |
US4427782A (en) * | 1981-03-03 | 1984-01-24 | Caldwell Harlan D | Isolation of principal outer membrane protein and antigen of Chlamydia trachomatis |
US4497899A (en) * | 1982-04-12 | 1985-02-05 | Abbott Laboratories | Immunoassay for Chlamydia trachomatis antigens |
FI69639C (fi) * | 1982-07-02 | 1986-03-10 | Orion Yhtymae Oy | Preparat foer anvaendning vid klamydia-diagnostik |
US4617264A (en) * | 1983-11-04 | 1986-10-14 | Syntex (U.S.A.) Inc. | Pretreatment method and composition |
US4663291A (en) * | 1984-07-06 | 1987-05-05 | Becton, Dickinson And Company | Method for solubilizing microbial protein obtained from Chlamydia trachomatis |
US4766065A (en) * | 1984-08-23 | 1988-08-23 | Becton Dickinson And Company | Detection of cell membrane protein |
GB8427006D0 (en) * | 1984-10-25 | 1984-11-28 | Iq Bio Ltd | Determination of chlamydia trachomatis |
EP0280560B1 (en) * | 1987-02-27 | 1994-05-11 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Membrane structure coated with low pI protein and methods of making and using |
US4916057A (en) * | 1988-02-29 | 1990-04-10 | Kallestad Diagnostics, Inc. | Chlamydia assay employing base treatment |
US5075221A (en) * | 1988-10-07 | 1991-12-24 | Eastman Kodak Company | Stabilized extraction composition containing a sulfhydryl-containing reducing agent and its use in chlamydial and gonococcal determinations |
US4978632A (en) * | 1988-12-15 | 1990-12-18 | Kallestad Diagnostics, Inc. | Method of pre-treating samples in peroxidase-catalyzed enzyme assays |
US5219890A (en) * | 1989-07-11 | 1993-06-15 | Wave Energy Systems, Inc. | Odorless Mycobactericidal compositions |
US5187066A (en) * | 1990-02-14 | 1993-02-16 | Syntex (U.S.A.) Inc. | Methods for detecting amphiphilic antigens |
US5187107A (en) * | 1991-06-27 | 1993-02-16 | Bio-Rad Laboratories, Inc. | B12 enzyme imunoassay and sample pretreatment |
-
1993
- 1993-05-19 US US08/065,019 patent/US5484706A/en not_active Expired - Fee Related
-
1994
- 1994-05-06 CA CA002123069A patent/CA2123069C/en not_active Expired - Fee Related
- 1994-05-06 DE DE4416144A patent/DE4416144A1/de not_active Ceased
- 1994-05-16 FR FR9405945A patent/FR2705463B1/fr not_active Expired - Fee Related
- 1994-05-18 ES ES09401086A patent/ES2066739B1/es not_active Expired - Fee Related
- 1994-05-18 IT ITTO940399A patent/IT1274006B/it active IP Right Grant
- 1994-05-18 CN CN94105532A patent/CN1082663C/zh not_active Expired - Fee Related
- 1994-05-18 JP JP10370594A patent/JP3335760B2/ja not_active Expired - Fee Related
- 1994-05-19 GB GB9410054A patent/GB2278195B/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0148166A2 (en) * | 1984-01-03 | 1985-07-10 | Georgetown University | Breast cancer diagnostic blood test |
US4536478A (en) * | 1984-04-05 | 1985-08-20 | Seragan Diagnostics, Inc. | Method for reducing non-specific interferences in agglutination immunoassays |
US4703001A (en) * | 1985-10-23 | 1987-10-27 | Synbiotics, Corporation | Immunoassay for the detection of serum analytes using pH dependent chastropic acids |
WO1989005975A1 (en) * | 1987-12-16 | 1989-06-29 | Microgenics Corporation | Serum pretreatment in vitamin b12 assay |
EP0599417A2 (en) * | 1992-11-25 | 1994-06-01 | Akzo Nobel N.V. | Method to dissociate an immunocomplex and immunoassay using it |
Also Published As
Publication number | Publication date |
---|---|
DE4416144A1 (de) | 1994-11-24 |
US5484706A (en) | 1996-01-16 |
CA2123069A1 (en) | 1994-11-20 |
JP3335760B2 (ja) | 2002-10-21 |
FR2705463B1 (fr) | 1997-04-11 |
CN1082663C (zh) | 2002-04-10 |
GB2278195A (en) | 1994-11-23 |
GB9410054D0 (en) | 1994-07-06 |
ES2066739B1 (es) | 1995-11-01 |
JPH06347460A (ja) | 1994-12-22 |
GB2278195B (en) | 1997-03-05 |
CN1104773A (zh) | 1995-07-05 |
CA2123069C (en) | 2006-07-04 |
ITTO940399A0 (it) | 1994-05-18 |
FR2705463A1 (fr) | 1994-11-25 |
IT1274006B (it) | 1997-07-14 |
ITTO940399A1 (it) | 1995-11-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PC2A | Transfer of patent | ||
FD1A | Patent lapsed |
Effective date: 20070519 |