KR950703061A - Fk506-결합 단백질을 인식하는 모노클로날 항체, fk506-결합 단백질의 농도를 분석하는 방법 및 이를 위한 키트(monoclonal antibody recognizing fk506-binding protein, method for assaying fk506-binding protein level, and kit therefor) - Google Patents

Fk506-결합 단백질을 인식하는 모노클로날 항체, fk506-결합 단백질의 농도를 분석하는 방법 및 이를 위한 키트(monoclonal antibody recognizing fk506-binding protein, method for assaying fk506-binding protein level, and kit therefor) Download PDF

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KR950703061A
KR950703061A KR1019950700510A KR19950700510A KR950703061A KR 950703061 A KR950703061 A KR 950703061A KR 1019950700510 A KR1019950700510 A KR 1019950700510A KR 19950700510 A KR19950700510 A KR 19950700510A KR 950703061 A KR950703061 A KR 950703061A
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binding protein
fkbp
monoclonal antibody
assaying
enzyme
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KR1019950700510A
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KR100243536B1 (ko
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코바야시 마사카주
오츠카 가즈유키
타나카 히로카즈
니와 미네오
Original Assignee
후지야마 아키라
후지사와 야꾸힝 고교 가부시키가이샤(Fujisawa Pharmaceutical Co., Ltd.)
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
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  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
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  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 FK506-결합 단백질내의 항원 결정군을 인식하지만 FK506-결합 단백질과 FK506 사이의 결합을 저해하지는 않는 모노클로날 항체(안티-FKBP항체); 고정화된 안티-FKBP 항체, 효소-표지된 FK506 및 검체중의 FKBP를 반응시키고 효소기질의 발색도를 분석하여 혈장중의 FKBP 농도를 분석하는 방법; 및 분석용 키트에 관한 것이다.
본 발명에 따르면 FK506의 면역억제작용에 영향을 미치는 혈장중의 FKBP 농도를 용이하고 더욱 정확하게 분석할 수 있고, 따라서 개개 환자의 조건에 따라 더욱 적합한 FK506의 최적용량을 결정할 수 있게 된다.

Description

FK506-결합 단백질을 인식하는 모노클로날 항체, FK506-결합 단백질의 농도를 분석하는 방법 및 이를 위한 키트(MONOCLONAL ANTIBODY RECOGNIZING FK506-BINDING PROTEIN, METHOD FOR ASSAYING FK506-BINDING PROTEIN LEVEL, AND KIT THEREFOR)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (5)

  1. FK506-결합 단백질내의 항원 결정군을 인식하지만 FK506-결합 단백질과 FK506 사이의 결합을 저해하지 않는 모노클로날 항체.
  2. 제1항에 있어서, FK506-결합 단백질이 인간 FKBP-12인 모노클로날 항체.
  3. 제1항에 따르는 고정화된 모노클로날 항체를 효소-표지된 FK506 및 검체중의 FK506-결합 단백질과 반응시킴을 특징으로 하여 FK506-결합 단백질의 농도를 분석하는 방법.
  4. 고정화된 FK506-결합 단백질을 효소-표지된 FK506 및 검체중의 FK506-결합 단백질과 반응시킴을 특징으로 하여 FK506-결합 단백질의 농도를 분석하는 방법.
  5. 제1항에 따르는 모노클로날 항체, FK506-결합 단백질 및 효소-표지된 FK506을 포함하는 FK506-결합 단백질 농도 분석용 키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950700510A 1992-08-12 1993-08-11 Fk506-결합 단백질을 인식하는 모노클로날 항체, fk506-결합 단백질의 농도를 분석하는 방법 및 이를 위한 키트 KR100243536B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP21496792 1992-08-12
JP214967/1992 1992-08-12
PCT/JP1993/001136 WO1994004700A1 (en) 1992-08-12 1993-08-11 Monoclonal antibody recognizing fk506-binding protein, method of assaying fk506-binding protein level, and kit thereof

Publications (2)

Publication Number Publication Date
KR950703061A true KR950703061A (ko) 1995-08-23
KR100243536B1 KR100243536B1 (ko) 2000-02-01

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KR1019950700510A KR100243536B1 (ko) 1992-08-12 1993-08-11 Fk506-결합 단백질을 인식하는 모노클로날 항체, fk506-결합 단백질의 농도를 분석하는 방법 및 이를 위한 키트

Country Status (12)

Country Link
US (1) US5576183A (ko)
EP (1) EP0672756B1 (ko)
JP (1) JP3185221B2 (ko)
KR (1) KR100243536B1 (ko)
AT (1) ATE213272T1 (ko)
AU (1) AU667743B2 (ko)
CA (1) CA2142197C (ko)
DE (1) DE69331585T2 (ko)
DK (1) DK0672756T3 (ko)
ES (1) ES2169068T3 (ko)
PT (1) PT672756E (ko)
WO (1) WO1994004700A1 (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3993695A (en) * 1994-12-07 1996-06-26 Fujisawa Pharmaceutical Co., Ltd. Method of measuring the concentration of fk506-binding protein
CA2318435A1 (en) * 1998-01-09 1999-07-15 Human Genome Sciences, Inc. Human fk506 binding proteins
US6492325B1 (en) * 1998-05-22 2002-12-10 Boys Town National Research Hospital Use of α1β1 integrin receptor inhibitors and TGF-β1 inhibitors in the treatment of kidney disease
US7078495B1 (en) 1999-08-03 2006-07-18 Dade Behring Inc. Monoclonal antibodies to tacrolimus and immunoassay methods for tacrolimus
CN1301770A (zh) * 1999-12-29 2001-07-04 复旦大学 一种新的多肽——人fkbp蛋白11和编码这种多肽的多核苷酸
US7186518B2 (en) * 2003-11-21 2007-03-06 Dade Behring Inc. Method and composition useful for determining FK 506
DE602006012033D1 (de) * 2005-04-06 2010-03-18 Abbott Lab Verfahren zur messung immunsuppressiver tacrolimus-, sirolimus- und cyclosporin-a-komplexe in einer blutprobe
KR101167649B1 (ko) 2011-04-20 2012-07-20 한국과학기술원 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 장치

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4894366A (en) * 1984-12-03 1990-01-16 Fujisawa Pharmaceutical Company, Ltd. Tricyclo compounds, a process for their production and a pharmaceutical composition containing the same
WO1993007269A1 (en) * 1991-10-11 1993-04-15 Vertex Pharmaceuticals Incorporated ISOLATION OF AN Mr 52,000 FK506 BINDING PROTEIN AND MOLECULAR CLONING OF A CORRESPONDING HUMAN cDNA

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ATE213272T1 (de) 2002-02-15
CA2142197C (en) 2004-06-22
CA2142197A1 (en) 1994-03-03
EP0672756B1 (en) 2002-02-13
AU667743B2 (en) 1996-04-04
EP0672756A1 (en) 1995-09-20
DE69331585T2 (de) 2002-08-08
ES2169068T3 (es) 2002-07-01
US5576183A (en) 1996-11-19
JP3185221B2 (ja) 2001-07-09
DK0672756T3 (da) 2002-03-25
DE69331585D1 (de) 2002-03-21
WO1994004700A1 (en) 1994-03-03
EP0672756A4 (en) 1996-10-09
PT672756E (pt) 2002-07-31
KR100243536B1 (ko) 2000-02-01
AU4761393A (en) 1994-03-15

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