AR000215A1 - Proceso mejorado para detectar polimorfismos entredos muestras de ácidos nucleicos individuales - Google Patents

Proceso mejorado para detectar polimorfismos entredos muestras de ácidos nucleicos individuales

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Publication number
AR000215A1
AR000215A1 AR33440995A AR33440995A AR000215A1 AR 000215 A1 AR000215 A1 AR 000215A1 AR 33440995 A AR33440995 A AR 33440995A AR 33440995 A AR33440995 A AR 33440995A AR 000215 A1 AR000215 A1 AR 000215A1
Authority
AR
Argentina
Prior art keywords
compound
primers
nucleic acid
improved process
acid samples
Prior art date
Application number
AR33440995A
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English (en)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR000215A1 publication Critical patent/AR000215A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se presentan compuestos microsatelitales utilizados como iniciadores autoanclantes para la exploración de genomas con el fin de detectarpolimorfismos genéticos. Los iniciadores microsatelitales compuestos preferidos son iniciadores dinucleotídicos compuestos perfectos, en los que los paresdinucleotídicos adyacentes contienen un nucleótido común que está en fase a través de la unión del compuesto. En una realización práctica, estosiniciadores compuestos en fase han sido adaptados a otros métodos conocidos de idintificación, incluyendo variaciones de Iso ensayos AFLP.
AR33440995A 1994-11-28 1995-11-28 Proceso mejorado para detectar polimorfismos entredos muestras de ácidos nucleicos individuales AR000215A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US34645694A 1994-11-28 1994-11-28

Publications (1)

Publication Number Publication Date
AR000215A1 true AR000215A1 (es) 1997-05-21

Family

ID=23359478

Family Applications (1)

Application Number Title Priority Date Filing Date
AR33440995A AR000215A1 (es) 1994-11-28 1995-11-28 Proceso mejorado para detectar polimorfismos entredos muestras de ácidos nucleicos individuales

Country Status (10)

Country Link
US (1) US5955276A (es)
EP (1) EP0804618B1 (es)
JP (1) JPH10509594A (es)
AR (1) AR000215A1 (es)
AU (1) AU704660B2 (es)
DE (1) DE69507646T2 (es)
IL (1) IL116135A0 (es)
NZ (1) NZ298236A (es)
WO (1) WO1996017082A2 (es)
ZA (1) ZA9510097B (es)

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DE19525284A1 (de) * 1995-06-28 1997-01-02 Inst Pflanzengenetik & Kultur Mikrosatellitenmarker für Pflanzen der Spezies Triticum aestivum sowie des Tribus Triticeae und ihre Verwendung
US6218119B1 (en) 1996-01-16 2001-04-17 Keygene, N. V. Amplification of simple sequence repeats
KR20010005544A (ko) * 1997-03-21 2001-01-15 그레그 펄쓰 Ｖｎｔｒ 대립 유전자의 추출 및 이용방법
EP0970246A1 (en) * 1997-03-21 2000-01-12 Greg Firth Extraction and utilisation of vntr alleles
ATE545710T1 (de) 1997-04-01 2012-03-15 Illumina Cambridge Ltd Verfahren zur vervielfältigung von nukleinsäuren
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
AU3747099A (en) 1998-04-16 1999-11-01 Case Western Reserve University Method for finding genetic markers of somaclonal variation
AR021815A1 (es) 1998-09-18 2002-08-07 Univ Nebraska Lincoln Analisis de alta resolucion de genomas.
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
WO2000023620A1 (en) * 1998-10-16 2000-04-27 Keygene N.V. Method for the generation of dna fingerprints
WO2000028081A2 (en) * 1998-11-09 2000-05-18 Methexis N.V. Restricted amplicon analysis
AU1696800A (en) * 1998-12-04 2000-06-26 Keygene N.V. Array and method for analysing nucleic acid sequences
DE19911130A1 (de) 1999-03-12 2000-09-21 Hager Joerg Verfahren zur Identifikation chromosomaler Regionen und Gene
US7166432B2 (en) 1999-03-12 2007-01-23 Integragen Compositions and methods for genetic analysis
EP1173612B1 (en) * 1999-04-09 2004-09-29 Keygene N.V. METHOD FOR THE DETECTION AND/OR ANALYSIS, BY MEANS OF PRIMER EXTENSION TECHNIQUES, OF SINGLE NUCLEOTIDE POLYMORPHISMS IN RESTRICTION FRAGMENTS, IN PARTICULAR IN AMPLIFIED RESTRICTION FRAGMENTS GENERATED USING AFLP$m(3)
US6420117B1 (en) 1999-09-14 2002-07-16 The University Of Georgia Research Foundation, Inc. Miniature inverted repeat transposable elements and methods of use
EP1130114A1 (en) * 2000-03-03 2001-09-05 Dr. van Haeringen Laboratorium B.V. Universal variable fragments
US20040014056A1 (en) * 2000-05-02 2004-01-22 Jorg Hager Identification of genetic markers
EP1158056A1 (en) * 2000-05-15 2001-11-28 Keygene N.V. Microsatellite-AFLP
EP1282729A2 (en) * 2000-05-15 2003-02-12 Keygene N.V. Microsatellite-aflp
EP1170379A1 (en) * 2000-06-30 2002-01-09 Centre National de Genotypage Sample generation for genotyping by mass spectrometry
US7695901B2 (en) * 2000-07-26 2010-04-13 North Carolina State University Identification of poinsettia cultivars
FR2822711B1 (fr) * 2001-03-28 2003-06-13 Oreal Dispositif de traitement comportant une enveloppe definissant une cavite dans laquelle peut-etre engagee une partie du corps
US7176303B2 (en) * 2003-11-06 2007-02-13 Isis Pharmaceuticals, Inc. Modulation of STAT5 expression
US20040110142A1 (en) * 2002-12-09 2004-06-10 Isis Pharmaceuticals Inc. Modulation of AAC-11 expression
US7115370B2 (en) * 2002-06-05 2006-10-03 Capital Genomix, Inc. Combinatorial oligonucleotide PCR
JPWO2004044243A1 (ja) * 2002-11-13 2006-03-09 株式会社日立ハイテクノロジーズ 核酸の増幅方法及びその装置
EP1687323A4 (en) * 2003-08-08 2009-08-12 Life Technologies Corp METHODS AND COMPOSITIONS FOR DIRECT CLONING OF NUCLEIC ACID MOLECULES
KR100645445B1 (ko) 2004-09-09 2006-11-15 강원대학교산학협력단 식물체의 다형성 검출용 마커의 제조방법
EP2292788B1 (en) 2005-06-23 2012-05-09 Keygene N.V. Strategies for high throughput identification and detection of polymorphisms
DK1929039T4 (en) 2005-09-29 2014-02-17 Keygene Nv High throughput-screening af mutageniserede populationer
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
EP3404114B1 (en) 2005-12-22 2021-05-05 Keygene N.V. Method for high-throughput aflp-based polymorphism detection
ES2301342B1 (es) * 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "metodo de analisis de acidos nucleicos".
EP3239304B1 (en) * 2006-04-04 2020-08-19 Keygene N.V. High throughput detection of molecular markers based on aflp and high troughput sequencing
DE102007010311A1 (de) * 2007-02-23 2008-08-28 Thines, Marco, Dr. Organismusspezifisches hybridisierbares Nucleinsäuremolekül
US20140053294A1 (en) * 2010-11-19 2014-02-20 The University Of Queensland Pongamia Genetic Markers and Method of Use
CN110172525A (zh) * 2019-06-26 2019-08-27 广西壮族自治区林业科学研究院 林木差异表达基因ssr引物组及多态性ssr标记开发方法
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US5206137A (en) * 1988-09-08 1993-04-27 Lifecodes Corporation Compositions and methods useful for genetic analysis
DE3834636A1 (de) * 1988-10-11 1990-04-19 Max Planck Gesellschaft Verfahren zur analyse von laengenpolymorphismen in dna-bereichen
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
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Also Published As

Publication number Publication date
WO1996017082A3 (en) 1996-08-08
JPH10509594A (ja) 1998-09-22
IL116135A0 (en) 1996-01-31
AU4367496A (en) 1996-06-19
EP0804618B1 (en) 1999-01-27
ZA9510097B (en) 1997-05-28
NZ298236A (en) 1999-01-28
DE69507646T2 (de) 1999-09-16
DE69507646D1 (de) 1999-03-11
AU704660B2 (en) 1999-04-29
EP0804618A2 (en) 1997-11-05
WO1996017082A2 (en) 1996-06-06
US5955276A (en) 1999-09-21

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