CN103461395A - Natural bactericide for controlling ginkgo blight and preparation method thereof - Google Patents
Natural bactericide for controlling ginkgo blight and preparation method thereof Download PDFInfo
- Publication number
- CN103461395A CN103461395A CN2013104447255A CN201310444725A CN103461395A CN 103461395 A CN103461395 A CN 103461395A CN 2013104447255 A CN2013104447255 A CN 2013104447255A CN 201310444725 A CN201310444725 A CN 201310444725A CN 103461395 A CN103461395 A CN 103461395A
- Authority
- CN
- China
- Prior art keywords
- ginkgo
- parts
- bactericidal agent
- die back
- grams
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention provides a natural bactericide for controlling ginkgo blight. The natural bactericide is prepared by the following components: equisetum, cassia twig, herba cephalanoploris, caulis polygoni multiflori, coptis, asarum herb, duchesnea indica, fructus polygoni orientalis and composite multi-mineral. The natural bactericide for controlling ginkgo blight is abundant in raw material source and low in cost, is prepared by the pure natural components, has no toxicity and no pollutions, and is capable of effectively controlling ginkgo blight.
Description
Technical field
The invention belongs to the prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent of preventing and treating the ginkgo die back disease and preparation method thereof.
Background technology
The ginkgo die back disease is one of Major Diseases of ginkgo, has seen at present Hebei, Henan, Shaanxi, Shandong, Jiangsu, Kang, Jiangxi, Zhejiang, Guangxi etc. and has economized (autonomous region).Except the harm ginkgo, also endanger the seeds such as Chinese chestnut.Disease occurs on trunk and branch, susceptible after, scab surrounds rapidly limb, often causes whole branch or complete stool death.Ginkgo die back disease germ, after wound is invaded trunk or branch, produces the scab of variable color on smooth bark, and scab is circular or irregular shape, and on coarse bark, the scab edge is not obvious.Scab continues expansion later, and enlargement gradually, the bark longitudinal cracking.Spring is visible many withered yellow excipuliform stromas on the bark of being injured, diameter 1～3mm.When weather is moist, can be from extruding the faint yellow or yellow cirrhiform conidium angle of a rule in the excipuliform stroma.Stroma becomes salmon pink to red sauce after autumn.Gradually form perithecium thereafter.Between sick tree bark and xylem, the fan-shaped subiculum of featheriness being arranged as seen, just is dirty white, is yellowish-brown afterwards.The generation reason of ginkgo seedling damping off it has been generally acknowledged that by chestnut epidemic disease shoot blight bacterium Endothiaparasitica (Murr.) and.et and. and infects and cause. the conidium of pathogen and ascospore all can play dissemination.This bacterium survives the winter on sick branch with mycelium and pycnidium, and when next spring warmth degree gos up, pathogen comes into play.The Yangtze river basin and areas to the south, the Changjiang river late March start to occur symptom to early April, and after late June, scab obviously enlarges, especially the fastest with scab expansion in 7～September.In the Yangtze river basin, the asexual generation of pathogen started to occur in the first tenday period of a month in late April to May.Mitogenetic spore is in borrowing rainwater, insect; Birds are propagated everywhere and can repeatedly infect again.In 10～November, occur burying and be born in pyrenocarpic salmon pink in seat on bark, early December the ascospore maturation.Ascospore is borrowed hearsay to broadcast germ and is invaded from host's wound.Sick subcortical fan-shaped subiculum, have very strong resistance to poor environment, the existence of can surviving the winter.Therefore, this germ is difficult for prevention and control.
For control ginkgo die back disease is normally used, be the chemical pesticide bactericide, because a large amount of problem of environmental pollutions that use chemical pesticide to cause are on the rise.Simultaneously, adopt the chemical pesticide bactericide, make various phytopathogen bacterial classifications produce drug resistance, pesticide resistance, increased the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, to useful biology, can threaten yet.And a lot of chemical pesticides are non-degradables or are difficult to degraded, easily at the animals and plants cylinder accumulation, pollute the enrichment of thing.
Along with the public is more and more higher to the requirement of " pollution-free food ", " pollution-free food ", but also increase gradually to the ecological agricultural chemical demand of pollution-free natural degradation in market.From the development of pesticide science, on pesticide manufacturing is used, " Harmless " of " back to nature " and agricultural chemicals, be the inexorable trend of society and Development of Natural Science, so the new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the purpose of this invention is to provide a kind of environmental protection, control ginkgo die back disease that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent of preventing and treating the ginkgo die back disease provided by the invention, by following component, made: scouring rush, cassia twig, field thistle, Polygonum multiflower knotweed, the coptis, the root of Chinese wild ginger, peppermint, mock-strawberry, fructus polygoni orientalis, compound many ore deposits.
Preferably, by the component of following weight portion, made: scouring rush 4-6 part, cassia twig 4-6 part, field thistle 4-6 part, Polygonum multiflower knotweed 4-6 part, coptis 2-4 part, root of Chinese wild ginger 1-3 part, peppermint 1-3 part, mock-strawberry 1-3 part, fructus polygoni orientalis 0.5-1.5 part, compound many ore deposits 1-3 part.
More preferably, by the component of following weight portion, made: 5 parts of scouring rushes, 5 parts of cassia twig, 5 parts of field thistles, 5 parts of Polygonum multiflower knotweeds, 3 parts of the coptiss, 2 parts of the roots of Chinese wild ginger, 2 parts of peppermints, 2 parts of mock-strawberrys, 1 part of fructus polygoni orientalis, 2 parts, compound many ore deposits.
Wherein, described compound many ore deposits comprise boron, zinc, molybdenum, manganese, copper, iron, and the compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams, surplus is the elements such as carbon, oxygen, nitrogen, phosphorus.
The present invention also provides the preparation method of the natural bactericidal agent of above-mentioned control ginkgo die back disease, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is scouring rush, cassia twig, field thistle, Polygonum multiflower knotweed, the coptis, the root of Chinese wild ginger, peppermint, mock-strawberry, fructus polygoni orientalis;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-80 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-80 ℃ and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo die back disease.
The present invention also provides the application of natural bactericidal agent in control ginkgo die back disease of above-mentioned control ginkgo die back disease, and be specially: the natural bactericidal agent that will prevent and treat the ginkgo die back disease mixes and is the sterilization microbial inoculum with 500-1000 times of water gaging.
Beneficial effect: the natural bactericidal agent raw material sources of control ginkgo die back disease provided by the invention are abundant, with low cost, adopt the component of pure natural to make, Nonpoisonous, non-environmental-pollution not only, and can effectively prevent and treat the ginkgo die back disease.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described concrete material proportion of embodiment, process conditions and result thereof be only for the present invention is described, and should also can not limit the present invention described in detail in claims.
Embodiment 1
The natural bactericidal agent of control ginkgo die back disease, made by the component of following weight portion: 4 parts of scouring rushes, 5 parts of cassia twig, 4 parts of field thistles, 5 parts of Polygonum multiflower knotweeds, 2 parts of the coptiss, 1 part of the root of Chinese wild ginger, 3 parts of peppermints, 3 parts of mock-strawberrys, 1.5 parts of fructus polygoni orientaliss, 2 parts, compound many ore deposits.The compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams.
Its preparation method comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is scouring rush, cassia twig, field thistle, Polygonum multiflower knotweed, the coptis, the root of Chinese wild ginger, peppermint, mock-strawberry, fructus polygoni orientalis;
(2) compound is added to the ethanol water of 8 times of amounts 95%, be heated to 60 ℃ and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times of amounts 95%, is heated to 60 ℃ and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into ethanol and add 1/15 of total amount, adds the water of 2 times of volumes of concentrate, standing 36h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo die back disease.
Embodiment 2
The natural bactericidal agent of control ginkgo die back disease, made by the component of following weight portion: 6 parts of scouring rushes, 6 parts of cassia twig, 6 parts of field thistles, 4 parts of Polygonum multiflower knotweeds, 4 parts of the coptiss, 3 parts of the roots of Chinese wild ginger, 1 part of peppermint, 1 part of mock-strawberry, 0.5 part of fructus polygoni orientalis, 1 part, compound many ore deposits.The compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams.
Its preparation method comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is scouring rush, cassia twig, field thistle, Polygonum multiflower knotweed, the coptis, the root of Chinese wild ginger, peppermint, mock-strawberry, fructus polygoni orientalis;
(2) compound is added to the ethanol water of 5 times of amounts 95%, be heated to 80 ℃ and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times of amounts 95%, is heated to 80 ℃ and decocts 4h;
(4) filter, merging filtrate, be concentrated into ethanol and add 1/20 of total amount, adds the water of 1 times of volume of concentrate, standing 48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo die back disease.
Embodiment 3
The natural bactericidal agent of control ginkgo die back disease, made by the component of following weight portion: 5 parts of scouring rushes, 4 parts of cassia twig, 5 parts of field thistles, 6 parts of Polygonum multiflower knotweeds, 3 parts of the coptiss, 2 parts of the roots of Chinese wild ginger, 2 parts of peppermints, 2 parts of mock-strawberrys, 1 part of fructus polygoni orientalis, 3 parts, compound many ore deposits.The compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams.
Its preparation method comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is scouring rush, cassia twig, field thistle, Polygonum multiflower knotweed, the coptis, the root of Chinese wild ginger, peppermint, mock-strawberry, fructus polygoni orientalis;
(2) compound is added to the ethanol water of 10 times of amounts 95%, be heated to 50 ℃ and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times of amounts 95%, is heated to 50 ℃ and decocts 2h;
(4) filter, merging filtrate, be concentrated into ethanol and add 1/10 of total amount, adds the water of 3 times of volumes of concentrate, standing 24h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo die back disease.
The inhibition activity of the natural bactericidal agent of the control ginkgo die back disease of embodiment 4 mensuration embodiment 1 to 3 to the strawberry pathogen
This experiment is in vitro, measures the natural bactericidal agent of control ginkgo die back disease of embodiment 1 to 3 to the inhibition activity of strawberry pathogen, and method and result are as follows:
1. for the examination bacterial classification
Chestnut epidemic disease shoot blight bacterium Endothiaparasitica (Murr.) and.et and. is commercially available
2.EC50 measure
Utilize mycelial growth rate method and spore germination method to measure the EC50 value of embodiment of the present invention 1-3 natural bactericidal agent to above-mentioned germ, the results are shown in Table 1.
Table 1 embodiment 1 natural bactericidal agent is to the EC50 value for the examination pathogen
Adopt method of regulation in " pesticide field efficacy medicine test criterion ", the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3 is tested, diluted concentration is 500 and 1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment ginkgo die back disease of embodiment 1 to 3, various diseases is respectively treated 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3
Claims (6)
1. a natural bactericidal agent of preventing and treating the ginkgo die back disease, is characterized in that: by following component, made: scouring rush, cassia twig, field thistle, Polygonum multiflower knotweed, the coptis, the root of Chinese wild ginger, peppermint, mock-strawberry, fructus polygoni orientalis, compound many ore deposits.
2. a kind of natural bactericidal agent of preventing and treating the ginkgo die back disease according to claim 1, it is characterized in that: the component by following weight portion is made: scouring rush 4-6 part, cassia twig 4-6 part, field thistle 4-6 part, Polygonum multiflower knotweed 4-6 part, coptis 2-4 part, root of Chinese wild ginger 1-3 part, peppermint 1-3 part, mock-strawberry 1-3 part, fructus polygoni orientalis 0.5-1.5 part, compound many ore deposits 1-3 part.
3. a kind of natural bactericidal agent of preventing and treating the ginkgo die back disease according to claim 1, it is characterized in that: the component by following weight portion is made: 5 parts of scouring rushes, 5 parts of cassia twig, 5 parts of field thistles, 5 parts of Polygonum multiflower knotweeds, 3 parts of the coptiss, 2 parts of the roots of Chinese wild ginger, 2 parts of peppermints, 2 parts of mock-strawberrys, 1 part of fructus polygoni orientalis, 2 parts, compound many ore deposits.
4. a kind of natural bactericidal agent of preventing and treating the ginkgo die back disease according to claim 1, it is characterized in that: described compound many ore deposits comprise boron, zinc, molybdenum, manganese, copper, iron, and the compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams.
5. a preparation method who prevents and treats the natural bactericidal agent of ginkgo die back disease is characterized in that: comprise the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is scouring rush, cassia twig, field thistle, Polygonum multiflower knotweed, the coptis, the root of Chinese wild ginger, peppermint, mock-strawberry, fructus polygoni orientalis;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-80 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-80 ℃ and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo die back disease.
6. the application of the natural bactericidal agent of control ginkgo die back disease in control ginkgo die back disease, it is characterized in that: the natural bactericidal agent that will prevent and treat the ginkgo die back disease mixes and is the sterilization microbial inoculum with 500-1000 times of water gaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310444725.5A CN103461395B (en) | 2013-09-26 | 2013-09-26 | A kind ofly prevent and treat natural bactericidal agent of ginkgo die back disease and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310444725.5A CN103461395B (en) | 2013-09-26 | 2013-09-26 | A kind ofly prevent and treat natural bactericidal agent of ginkgo die back disease and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103461395A true CN103461395A (en) | 2013-12-25 |
| CN103461395B CN103461395B (en) | 2015-08-05 |
Family
ID=49786636
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310444725.5A Active CN103461395B (en) | 2013-09-26 | 2013-09-26 | A kind ofly prevent and treat natural bactericidal agent of ginkgo die back disease and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103461395B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828657A (en) * | 2014-03-17 | 2014-06-04 | 刘备 | Biological prevention and control method for grape gray mold |
| CN107624806A (en) * | 2017-08-26 | 2018-01-26 | 贵州宏海丰林林业科技发展有限公司 | One kind preventing and treating peach gummosis medicament and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829276A (en) * | 2010-02-06 | 2010-09-15 | 泰一和浦（北京）中医药研究院有限公司 | Traditional Chinese medicine combination for treating pneumonoconiosis and preparation method thereof |
| CN101850097A (en) * | 2010-01-19 | 2010-10-06 | 泰一和浦（北京）中医药研究院有限公司 | Chinese medicinal composition for treating medial headache and migraine and preparation method thereof |
| CN102614440A (en) * | 2012-04-27 | 2012-08-01 | 伊彩虹 | Multi-herb antibacterial liquid |
-
2013
- 2013-09-26 CN CN201310444725.5A patent/CN103461395B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101850097A (en) * | 2010-01-19 | 2010-10-06 | 泰一和浦（北京）中医药研究院有限公司 | Chinese medicinal composition for treating medial headache and migraine and preparation method thereof |
| CN101829276A (en) * | 2010-02-06 | 2010-09-15 | 泰一和浦（北京）中医药研究院有限公司 | Traditional Chinese medicine combination for treating pneumonoconiosis and preparation method thereof |
| CN102614440A (en) * | 2012-04-27 | 2012-08-01 | 伊彩虹 | Multi-herb antibacterial liquid |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828657A (en) * | 2014-03-17 | 2014-06-04 | 刘备 | Biological prevention and control method for grape gray mold |
| CN107624806A (en) * | 2017-08-26 | 2018-01-26 | 贵州宏海丰林林业科技发展有限公司 | One kind preventing and treating peach gummosis medicament and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103461395B (en) | 2015-08-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103404541B (en) | Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide | |
| CN104041538B (en) | A kind ofly prevent and treat natural bactericidal agent of strawberry black spot and preparation method thereof | |
| CN104094950B (en) | There is the fungicidal composition of synergism | |
| CN103380790B (en) | Natural bactericide for preventing and curing peach gummosis and preparation method thereof | |
| CN104041547B (en) | A kind of natural bactericidal agent preventing and treating white leaf spot of strawberry and preparation method thereof | |
| CN104012581B (en) | A kind ofly prevent and treat natural bactericidal agent of strawberry zonate spot and preparation method thereof | |
| CN104026168B (en) | A kind of natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry and preparation method thereof | |
| CN106135318A (en) | A kind of Chinese medicine composition and its preparation method and application | |
| CN104996489A (en) | Natural bactericide for preventing and controlling pepper anthracnose | |
| CN103380789B (en) | Natural bactericide for preventing and curing pear scab and preparation method thereof | |
| CN103444729A (en) | Bactericidal pharmaceutical composition and applications thereof | |
| CN103329945A (en) | Natural bactericide for controlling peach tree anthracnose and preparation method | |
| CN104041530A (en) | Natural bactericide for controlling strawberry leaf blight and preparation method thereof | |
| CN102812972A (en) | Plant extract composition for preventing and treating apple tree canker, and preparation method thereof | |
| CN103461401B (en) | A kind ofly prevent and treat natural bactericidal agent of ginkgo dead leaf disease and preparation method thereof | |
| CN103518783B (en) | Natural bactericide for preventing and controlling ginkgo seedling stem rot and preparation method thereof | |
| CN105145641A (en) | Granules containing trichoderma harzianum and chitosan | |
| CN103461397B (en) | A kind of natural pesticide for gingko and preparation method thereof | |
| CN103340218A (en) | Natural bactericide for prevention and treatment of peach brown rot and preparation method thereof | |
| CN103461395B (en) | A kind ofly prevent and treat natural bactericidal agent of ginkgo die back disease and preparation method thereof | |
| CN100411523C (en) | Compound pesticide of triperygium wilfordii alkaloid | |
| CN103461399B (en) | A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof | |
| CN103380788A (en) | Natural pesticide used for nursery stocks and preparation method thereof | |
| CN110622982A (en) | Sterilization composition containing zhongshengmycin | |
| CN102715155B (en) | Artificially synthesized phyllocnistis citrella stainton gyplure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 325200 Wenzhou City, Ruian City, fairy street, the village of the first Village No. 115 Applicant after: Bian Jialin Address before: 212100, No. 4, No. 54, Henderson Pacific Business Center, Dantu New District, Jiangsu, Zhenjiang Applicant before: Bian Jialin |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 212100 ZHENJIANG, JIANGSU PROVINCE TO: 325200 WENZHOU, ZHEJIANG PROVINCE |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 113000 17-2-202, YuLongWan community, Gaowan street, Fushun Economic Development Zone, Fushun City, Liaoning Province Patentee after: Bian Jialin Address before: 325200 No. 115 Tong Tou village, Xian Jiang Street, Ruian, Wenzhou, Zhejiang Patentee before: Bian Jialin |