CN104041530A - Natural bactericide for controlling strawberry leaf blight and preparation method thereof - Google Patents
Natural bactericide for controlling strawberry leaf blight and preparation method thereof Download PDFInfo
- Publication number
- CN104041530A CN104041530A CN201410235002.9A CN201410235002A CN104041530A CN 104041530 A CN104041530 A CN 104041530A CN 201410235002 A CN201410235002 A CN 201410235002A CN 104041530 A CN104041530 A CN 104041530A
- Authority
- CN
- China
- Prior art keywords
- parts
- bactericidal agent
- strawberry leaves
- natural bactericidal
- leaves rot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A provided natural bactericide for controlling strawberry leaf blight is prepared from dendranthema lavandulifolium, common cnidium fruit, lotus leaf, pinus ponderosa, gleditsia sinensis, artemisia argyi, celastrus angulatus, pogostemon cablin, tetrapanax papyriferus and radix aucklandiae. The natural bactericide for controlling strawberry leaf blight is abundant in raw-material source and low in cost, is prepared by employing pure natural compositions, is non-toxic and free of public hazard, and also is capable of effectively controlling strawberry leaf blight.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent of preventing and treating strawberry leaves rot and preparation method thereof.
Background technology
Strawberry leaves rot claims again purple spot, focal spot disease, and China occurs commonplace, is one of strawberry leaves portion Common Diseases, harm occurs sometimes quite serious.Strawberry leaves rot cause of disease is pineapple strawberry brown patch germ Marssonina potentillae (Desmazieres) Magn, and the perfect stage is that Diplocarpon earliana (Ellet EV.) belongs to Ascomycotina.Germ, discharges ascospore or conidium spring and borrows air to spread propagation, infect morbidity in plant diseased tissues or land on invalid thing and survive the winter with perithecium or pycnidium, and in seedling carries out in spite of illness, long-distance communications.This disease belongs to low temperature properties disease, and autumn and the more weather of rain and dew in early spring are conducive to infect morbidity.The strong morbidity of fertile sufficient seedling is light, and the weak morbidity of fertilizer deficiency seedling is heavy.Between strawberry cultivars, disease resistance is variant, and new star, to reach the kinds such as Na more disease-resistant.
Strawberry leaves rot mainly, in morbidity in season in spring and autumn, is encroached on blade, petiole, carpopodium and calyx.On blade, produce the matt fleck of puce, be expanded into the irregular shape scab of 3-4 millimeters of diameters later, scab central authorities are little with periphery change color, scab has the tendency distributing along vein, when morbidity is heavy, blade face is covered with scab, and the later stage, full leaf was yellowish-brown to crineous, until withered.In the withered part of scab, grow black small grain point, after petiole or carpopodium morbidity, produce the scab that pitchy slightly caves in, sick portion tissue becomes fragile and frangibility.
At present, in vegetables, melon and fruit plantation, for control strawberry leaves rot is normally used, be chemical pesticide bactericide, because a large amount of problem of environmental pollutions that use chemical pesticide to cause are on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classifications produce drug resistances, pesticide resistance, increased the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, to useful biology, can threaten yet.And a lot of chemical pesticides are non-degradables or are difficult to degraded, easily pollute the enrichment of thing at animals and plants cylinder accumulation.
Along with the public is more and more higher to the requirement of " pollution-free food ", " pollution-free food ", also increase gradually to ecological agricultural chemical demand pollution-free, can natural degradation in market.From the development of pesticide science, on pesticide manufacturing is used, " Harmless " of " back to nature " and agricultural chemicals, be the inexorable trend of society and Development of Natural Science, so new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control strawberry leaves rot that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent of preventing and treating strawberry leaves rot provided by the invention, by following component, made: camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant, the banksia rose.
Component by following weight portion is made: camomile 10-20 part, frutus cnidii 8-12 part, lotus leaf 8-12 part, yellow pine 2-4 part, Chinese honey locust 2-4 part, tarragon 2-4 part, Celastrus angulatus 2-4 part, Pogostemon cablin 1-3 part, stem pith of the rice-paper plant 1-3 part, banksia rose 1-3 part.
Component by following weight portion is made: 15 parts of camomiles, 10 parts of frutus cnidiis, 10 parts, lotus leaf, 3 parts of yellow pines, 3 parts of Chinese honey locusts, 3 parts of tarragons, 3 parts of Celastrus angulatus, 2 parts of Pogostemon cablin, 2 parts of the stem piths of the rice-paper plant, 2 parts of the banksia rose.
The present invention also provides the preparation method of the natural bactericidal agent of above-mentioned control strawberry leaves rot, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-70 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-70 ℃ and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
The present invention also provides the application of the natural bactericidal agent of above-mentioned control strawberry leaves rot, is specially: the natural bactericidal agent of control strawberry leaves rot is mixed and is sterilization microbial inoculum with 500-1000 times of water gaging.
Beneficial effect: the natural bactericidal agent raw material sources of control strawberry leaves rot provided by the invention are abundant, with low cost, adopts the component of pure natural to make, Nonpoisonous, non-environmental-pollution not only, and can effectively prevent and treat strawberry leaves rot.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described concrete material proportion of embodiment, process conditions and result thereof be only for the present invention is described, and should also can not limit the present invention described in detail in claims.
Embodiment 1
The natural bactericidal agent of control strawberry leaves rot, is made by the component of following weight portion: 15 parts of camomiles, 10 parts of frutus cnidiis, 10 parts, lotus leaf, 3 parts of yellow pines, 3 parts of Chinese honey locusts, 3 parts of tarragons, 3 parts of Celastrus angulatus, 2 parts of Pogostemon cablin, 2 parts of the stem piths of the rice-paper plant, 2 parts of the banksia rose
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added to the ethanol water of 8 times of amounts 95%, be heated to 60 ℃ and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times of amounts 95%, is heated to 60 ℃ and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/15 of total amount, adds the water of 2 times of volumes of concentrate, standing 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
Embodiment 2
The natural bactericidal agent of control strawberry leaves rot, is made by the component of following weight portion: 20 parts of camomiles, 8 parts of frutus cnidiis, 8 parts, lotus leaf, 2 parts of yellow pines, 4 parts of Chinese honey locusts, 4 parts of tarragons, 4 parts of Celastrus angulatus, 3 parts of Pogostemon cablin, 3 parts of the stem piths of the rice-paper plant, 1 part of the banksia rose.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added to the ethanol water of 5 times of amounts 95%, be heated to 70 ℃ and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times of amounts 95%, is heated to 70 ℃ and decocts 4h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/20 of total amount, adds the water of 1 times of volume of concentrate, standing 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
Embodiment 3
The natural bactericidal agent of control strawberry leaves rot, is made by the component of following weight portion: 10 parts of camomiles, 12 parts of frutus cnidiis, 12 parts, lotus leaf, 4 parts of yellow pines, 2 parts of Chinese honey locusts, 2 parts of tarragons, 2 parts of Celastrus angulatus, 1 part of Pogostemon cablin, 1 part of the stem pith of the rice-paper plant, 3 parts of the banksia rose.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added to the ethanol water of 10 times of amounts 95%, be heated to 50 ℃ and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times of amounts 95%, is heated to 50 ℃ and decocts 2h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/10 of total amount, adds the water of 3 times of volumes of concentrate, standing 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
The natural bactericidal agent of the control strawberry leaves rot of embodiment 4 mensuration embodiment 1 to 3 is active to the inhibition of strawberry pathogen
This experiment is in vitro, and the natural bactericidal agent of the control strawberry leaves rot of mensuration embodiment 1 to 3 is active to the inhibition of strawberry pathogen, and method and result are as follows:
1. for examination bacterial classification
Pineapple strawberry brown patch germ Marssonina potentillae (Desmazieres) Magn is commercially available.
2. Toxicity Determination
Utilize mycelial growth rate method and spore germination method to measure the virulence of the embodiment of the present invention 1 natural bactericidal agent to pineapple strawberry brown patch germ Marssonina potentillae (Desmazieres) Magn, by following formula, calculate inhibiting rate:
3. experimental result
With growth rate method, measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for stipulating in < < pesticide field efficacy medicine test criterion > >, field efficacy to the natural bactericidal agent of the embodiment of the present invention 1 to 3 is tested, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
The natural bactericidal agent treatment strawberry leaves rot that adopts embodiment 1 to 3, various diseases is respectively treated 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry leaves rot of table 3 embodiment 1 to 3
Claims (5)
1. a natural bactericidal agent of preventing and treating strawberry leaves rot, is characterized in that: by following component, made: camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant, the banksia rose.
2. a kind of natural bactericidal agent of preventing and treating strawberry leaves rot according to claim 1, is characterized in that: the component by following weight portion is made: camomile 5-15 part, frutus cnidii 5-15 part, lotus leaf 8-12 part, yellow pine 8-12 part, Chinese honey locust 2-4 part, tarragon 2-4 part, Celastrus angulatus 2-4 part, Pogostemon cablin 1-3 part, stem pith of the rice-paper plant 1-3 part, banksia rose 1-3 part.
3. a kind of natural bactericidal agent of preventing and treating strawberry leaves rot according to claim 1, is characterized in that: the component by following weight portion is made: 10 parts of camomiles, 10 parts of frutus cnidiis, 10 parts, lotus leaf, 10 parts of yellow pines, 3 parts of Chinese honey locusts, 3 parts of tarragons, 3 parts of Celastrus angulatus, 2 parts of Pogostemon cablin, 2 parts of the stem piths of the rice-paper plant, 2 parts of the banksia rose.
4. a preparation method who prevents and treats the natural bactericidal agent of strawberry leaves rot, is characterized in that: comprise the following steps:
Raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
Add 5-10 doubly to measure 95% ethanol water compound, be heated to 50-70 ℃ and decoct 2-4h;
Filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-70 ℃ and decocts 2-4h;
Filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
Filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
5. the application of the natural bactericidal agent of control strawberry leaves rot in control strawberry leaves rot, is characterized in that: the natural bactericidal agent of control strawberry leaves rot is mixed and is sterilization microbial inoculum with 500-1000 times of water gaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410235002.9A CN104041530B (en) | 2014-05-29 | 2014-05-29 | A kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410235002.9A CN104041530B (en) | 2014-05-29 | 2014-05-29 | A kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104041530A true CN104041530A (en) | 2014-09-17 |
| CN104041530B CN104041530B (en) | 2016-01-20 |
Family
ID=51495399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410235002.9A Active CN104041530B (en) | 2014-05-29 | 2014-05-29 | A kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104041530B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010423A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Strawberry leaf blight prevention and treatment method |
| CN105028530A (en) * | 2015-06-02 | 2015-11-11 | 张亚南 | Botanical pesticide for strawberry production |
| CN105053082A (en) * | 2015-07-15 | 2015-11-18 | 刘晓 | Drug for preventing and treating michelia figo leaf blight |
| CN105994451A (en) * | 2016-07-29 | 2016-10-12 | 广西素安生态农业有限公司 | Chinese herbal medicine control agent for common diseases of strawberries and preparation method thereof |
| CN106070206A (en) * | 2016-06-14 | 2016-11-09 | 重庆鸿钜农业科技发展有限公司 | A kind of compositions preventing and treating lawn pinta in summer |
| CN106577719A (en) * | 2016-11-24 | 2017-04-26 | 广西大学 | Bactericide composite for preventing and controlling gingko leaf blight |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1452869A (en) * | 2002-04-26 | 2003-11-05 | 郭立卿 | Prepn of wide-spectrum biopesticide of Chinese herbal medicine |
| KR20090047563A (en) * | 2007-10-30 | 2009-05-13 | 전라남도 | Botanical pesticide from chrysanthemum spp. extract with insecticidal soap to control of strawberry pest |
| CN101869122A (en) * | 2010-06-01 | 2010-10-27 | 刘志慧 | Garlicin plant growth promoting solution with insect disinfestation and sterilization functions |
-
2014
- 2014-05-29 CN CN201410235002.9A patent/CN104041530B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1452869A (en) * | 2002-04-26 | 2003-11-05 | 郭立卿 | Prepn of wide-spectrum biopesticide of Chinese herbal medicine |
| KR20090047563A (en) * | 2007-10-30 | 2009-05-13 | 전라남도 | Botanical pesticide from chrysanthemum spp. extract with insecticidal soap to control of strawberry pest |
| CN101869122A (en) * | 2010-06-01 | 2010-10-27 | 刘志慧 | Garlicin plant growth promoting solution with insect disinfestation and sterilization functions |
Non-Patent Citations (2)
| Title |
|---|
| 余何森 等: "赣北山区草莓母株稀植春繁育苗技术", 《现代农村科技》 * |
| 无: "草莓册子文案", 《无》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028530A (en) * | 2015-06-02 | 2015-11-11 | 张亚南 | Botanical pesticide for strawberry production |
| CN105010423A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Strawberry leaf blight prevention and treatment method |
| CN105053082A (en) * | 2015-07-15 | 2015-11-18 | 刘晓 | Drug for preventing and treating michelia figo leaf blight |
| CN106070206A (en) * | 2016-06-14 | 2016-11-09 | 重庆鸿钜农业科技发展有限公司 | A kind of compositions preventing and treating lawn pinta in summer |
| CN105994451A (en) * | 2016-07-29 | 2016-10-12 | 广西素安生态农业有限公司 | Chinese herbal medicine control agent for common diseases of strawberries and preparation method thereof |
| CN106577719A (en) * | 2016-11-24 | 2017-04-26 | 广西大学 | Bactericide composite for preventing and controlling gingko leaf blight |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104041530B (en) | 2016-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104041547B (en) | A kind of natural bactericidal agent preventing and treating white leaf spot of strawberry and preparation method thereof | |
| CN104041538B (en) | A kind ofly prevent and treat natural bactericidal agent of strawberry black spot and preparation method thereof | |
| CN104041530B (en) | A kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof | |
| CN103404541B (en) | Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide | |
| CN103380790B (en) | Natural bactericide for preventing and curing peach gummosis and preparation method thereof | |
| CN103329945B (en) | Natural bactericide for controlling peach tree anthracnose and preparation method | |
| CN104012581B (en) | A kind ofly prevent and treat natural bactericidal agent of strawberry zonate spot and preparation method thereof | |
| CN104026168B (en) | A kind of natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry and preparation method thereof | |
| CN103380789B (en) | Natural bactericide for preventing and curing pear scab and preparation method thereof | |
| Ma et al. | The effects and health risk assessment of cauliflower co-cropping with Sedum alfredii in cadmium contaminated vegetable field | |
| CN104996489A (en) | Natural bactericide for preventing and controlling pepper anthracnose | |
| CN106259575A (en) | A kind of plant liquid extract and plant protecting liquid | |
| CN105379781A (en) | Natural bactericide for preventing and treating Anoectochilus roxburghii southern blight and preparation method of natural bactericide | |
| CN103518783B (en) | Natural bactericide for preventing and controlling ginkgo seedling stem rot and preparation method thereof | |
| CN105248478A (en) | Natural bactericide for preventing and treating loquat brown spot and preparation method thereof | |
| CN103461401B (en) | A kind ofly prevent and treat natural bactericidal agent of ginkgo dead leaf disease and preparation method thereof | |
| CN103461397B (en) | A kind of natural pesticide for gingko and preparation method thereof | |
| CN103461399B (en) | A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof | |
| CN103380788A (en) | Natural pesticide used for nursery stocks and preparation method thereof | |
| CN106665623A (en) | Sterilization composition containing picarbutrazox and application thereof | |
| CN103461395B (en) | A kind ofly prevent and treat natural bactericidal agent of ginkgo die back disease and preparation method thereof | |
| CN109430264B (en) | Bactericide composition containing terpene alcohol and chitosan, and preparation method and application thereof | |
| CN107041388A (en) | A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight and preparation method and application | |
| CN105454315A (en) | Pollution-free bactericide for preventing brown patch of myrica rubra and preparation method of pollution-free bactericide | |
| CN105379782A (en) | Natural bactericide for preventing and treating purple perilla root rot and preparation method of natural bactericide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |