CN103404541B - Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide - Google Patents
Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide Download PDFInfo
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- CN103404541B CN103404541B CN201310267831.0A CN201310267831A CN103404541B CN 103404541 B CN103404541 B CN 103404541B CN 201310267831 A CN201310267831 A CN 201310267831A CN 103404541 B CN103404541 B CN 103404541B
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Abstract
The invention provides a natural bactericide for preventing and treating strawberry anthracnose. The natural bactericide is prepared from the following components: eucalyptus robusta, folium artemisiae argyi, Chinese fevervine herb, acorus calamus, rhizoma coptidis, fruit of Rangoon creeper, radix isatidis, radix paeoniae alba, mint and garlic. The natural bactericide for preventing and treating strawberry anthracnose is rich in raw material sources and low in cost, and is prepared from pure natural components, so that the natural bactericide is non-toxic and pollution-free, and can be used for effectively preventing and treating the strawberry anthracnose.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent preventing and treating Strawberry anthracnose and preparation method thereof.
Background technology
Strawberry anthracnose is one of Major Diseases of Strawberry seedling.Strawberry anthracnose mainly occurs in nursery stage (stolon pumping phase) and field planting initial stage, and fruiting period seldom occurs.Its main harm stolon, petiole, blade, stipule, petal, calyx and fruit.Obvious characteristic after catching an illness be strawberry strain leaf be injured can cause local scab and complete stool wilt withered.Stolon, petiole, blade are caught an illness, and the black spindle of initial generation diameter 3 ~ 7 millimeters or oval ulcer shape scab, slightly cave in; When stolon and petiolar Spot expansion become annular ring, scab is wilted withered with upper part, the visible flesh pink clayey in disease portion sorus during humidity height.This disease except cause local scab except, also easily cause susceptible variety especially strawberry rice shoot wilt withered in flakes; When maternal plant phyllopodium and the morbidity of shortening stem position, initial 1 ~ 2 expanded leaves dehydration is sagging, and dusk or cloudy day recover normal, and along with aggravation, then complete stool is withered.Although do not occur that lobus cardiacus is downgraded and yellow symptom, observe if get cross section, withered diseased plant root cap portion, visible from outside to inside brown stain occurring, and the non-variable color of vascular bundle.Berry is injured, and produce subcircular scab, light brown is to crineous, and soft rotten shape also caves in, and the later stage also can grow flesh pink clayey sorus.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for preventing fungi normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control Strawberry anthracnose that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating Strawberry anthracnose provided by the invention, is made up of following component: eucalyptus robusta, tarragon, Chinese Fevervine Herb, calamus, the coptis, the fruit of Rangoon creeper, cloves, the root of herbaceous peony, peppermint, garlic.
Be made up of the component of following weight portion: eucalyptus robusta 4-6 part, tarragon 5-7 part, Chinese Fevervine Herb 4-6 part, calamus 4-6 part, coptis 2-4 part, fruit of Rangoon creeper 1-3 part, cloves 1-3 part, root of herbaceous peony 1-3 part, peppermint 0.5-1.5 part, garlic 0.5-1.5 part.
Be made up of the component of following weight portion: eucalyptus robusta 5 parts, tarragon 6 parts, Chinese Fevervine Herb 5 parts, calamus 5 parts, the coptis 3 parts, the fruit of Rangoon creeper 2 parts, cloves 2 parts, the root of herbaceous peony 2 parts, peppermint 1 part, 1 part, garlic.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control Strawberry anthracnose, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is eucalyptus robusta, tarragon, Chinese Fevervine Herb, calamus, the coptis, the fruit of Rangoon creeper, cloves, the root of herbaceous peony, peppermint and garlic;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Strawberry anthracnose.
Present invention also offers the application of natural bactericidal agent in control Strawberry anthracnose of above-mentioned control Strawberry anthracnose, be specially: the natural bactericidal agent of control Strawberry anthracnose is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control Strawberry anthracnose provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and effectively can prevent and treat Strawberry anthracnose.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control Strawberry anthracnose, is made up of the component of following weight portion: eucalyptus robusta 4 parts, tarragon 6 parts, Chinese Fevervine Herb 4 parts, calamus 5 parts, the coptis 2 parts, the fruit of Rangoon creeper 1 part, cloves 3 parts, the root of herbaceous peony 3 parts, peppermint 1.5 parts, 0.5 part, garlic.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is eucalyptus robusta, tarragon, Chinese Fevervine Herb, calamus, the coptis, the fruit of Rangoon creeper, cloves, the root of herbaceous peony, peppermint and garlic;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Strawberry anthracnose.
Embodiment 2
The natural bactericidal agent of control Strawberry anthracnose, is made up of the component of following weight portion: eucalyptus robusta 6 parts, tarragon 5 parts, Chinese Fevervine Herb 6 parts, calamus 4 parts, the coptis 4 parts, the fruit of Rangoon creeper 3 parts, cloves 1 part, the root of herbaceous peony 1 part, peppermint 0.5 part, 1.5 parts, garlic.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is eucalyptus robusta, tarragon, Chinese Fevervine Herb, calamus, the coptis, the fruit of Rangoon creeper, cloves, the root of herbaceous peony, peppermint and garlic;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 80 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 80 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Strawberry anthracnose.
Embodiment 3
The natural bactericidal agent of control Strawberry anthracnose, is made up of the component of following weight portion: eucalyptus robusta 5 parts, tarragon 7 parts, Chinese Fevervine Herb 5 parts, calamus 6 parts, the coptis 3 parts, the fruit of Rangoon creeper 2 parts, cloves 2 parts, the root of herbaceous peony 2 parts, peppermint 1 part, 1 part, garlic.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is eucalyptus robusta, tarragon, Chinese Fevervine Herb, calamus, the coptis, the fruit of Rangoon creeper, cloves, the root of herbaceous peony, peppermint and garlic;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Strawberry anthracnose.
Embodiment 4 measures the natural bactericidal agent of the control Strawberry anthracnose of embodiment 1 to 3 to the inhibit activities of strawberry pathogen
This experiment, in vitro, measures the natural bactericidal agent of control Strawberry anthracnose of embodiment 1 to 3 to the inhibit activities of strawberry pathogen, method and result as follows:
1. for examination bacterial classification
Strawberry colletotrichum gloeosporioides Penz (Colletotrichum gloeosporioides) is commercially available
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of above-mentioned germ, by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment Strawberry anthracnose of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3
Claims (4)
1. prevent and treat a natural bactericidal agent for Strawberry anthracnose, it is characterized in that: be made up of the component of following weight portion: eucalyptus robusta 4-6 part, tarragon 5-7 part, Chinese Fevervine Herb 4-6 part, calamus 4-6 part, coptis 2-4 part, fruit of Rangoon creeper 1-3 part, cloves 1-3 part, root of herbaceous peony 1-3 part, peppermint 0.5-1.5 part, garlic 0.5-1.5 part.
2. a kind of natural bactericidal agent preventing and treating Strawberry anthracnose according to claim 1, is characterized in that: be made up of the component of following weight portion: eucalyptus robusta 5 parts, tarragon 6 parts, Chinese Fevervine Herb 5 parts, calamus 5 parts, the coptis 3 parts, the fruit of Rangoon creeper 2 parts, cloves 2 parts, the root of herbaceous peony 2 parts, peppermint 1 part, 1 part, garlic.
3. a preparation method for the natural bactericidal agent of the control Strawberry anthracnose described in claim 1 or 2, is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is eucalyptus robusta, tarragon, Chinese Fevervine Herb, calamus, the coptis, the fruit of Rangoon creeper, cloves, the root of herbaceous peony, peppermint and garlic;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Strawberry anthracnose.
4. the application of natural bactericidal agent in control Strawberry anthracnose of the control Strawberry anthracnose described in claim 1 or 2, is characterized in that: mixed with 500-1000 times of water gaging by the natural bactericidal agent of control Strawberry anthracnose and be bactericide.
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Families Citing this family (16)
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| CN104642400B (en) * | 2015-01-23 | 2017-11-17 | 广西大学 | The purposes of southern biennial wormwood extract preventing and treating pseudo-ginseng anthracnose |
| CN104839238A (en) * | 2015-04-02 | 2015-08-19 | 广西大学 | Natural bactericide for preventing and treating anthracnose of edible lilies |
| CN105028530A (en) * | 2015-06-02 | 2015-11-11 | 张亚南 | Botanical pesticide for strawberry production |
| CN104938536A (en) * | 2015-06-04 | 2015-09-30 | 胡凡营 | Plant-derived biopesticide and preparation method thereof |
| CN104982469A (en) * | 2015-06-26 | 2015-10-21 | 安徽禾众农业科技有限公司 | Method for controlling strawberry anthracnose |
| CN105028519A (en) * | 2015-06-26 | 2015-11-11 | 安徽禾众农业科技有限公司 | Bacteriostatic agent for prevention and treatment of strawberry anthracnose and preparation method and application thereof |
| CN105053083A (en) * | 2015-07-15 | 2015-11-18 | 刘晓 | Drug for preventing and treating michelia figo anthracnose |
| CN105053075A (en) * | 2015-07-20 | 2015-11-18 | 句容市后白镇解氏家庭农场 | Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide |
| CN104996492A (en) * | 2015-07-20 | 2015-10-28 | 句容市后白镇解氏家庭农场 | Natural bactericidal agent for preventing and controlling loquat canker, and preparation method thereof |
| CN105493997A (en) * | 2015-12-04 | 2016-04-20 | 安徽省康之源农业科技有限公司 | Nuisanceless strawberry anthracnose prevention and treatment method |
| CN106070373A (en) * | 2016-06-02 | 2016-11-09 | 蚌埠市燕山草莓专业合作社 | A kind of Strawberry seedling anthrax control agent |
| CN105994451A (en) * | 2016-07-29 | 2016-10-12 | 广西素安生态农业有限公司 | Chinese herbal medicine control agent for common diseases of strawberries and preparation method thereof |
| CN106472592A (en) * | 2016-10-13 | 2017-03-08 | 合肥润雨农业科技有限公司 | A kind of anthrax pesticide control of Flos Osmanthi Fragrantis |
| CN106577852A (en) * | 2016-11-14 | 2017-04-26 | 广西素安生态农业有限公司 | Chinese herbal medicinal bactericide for crops, and preparation method thereof |
| CN107047658A (en) * | 2017-05-03 | 2017-08-18 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating safron anthracnose |
| CN106973961A (en) * | 2017-05-03 | 2017-07-25 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating safron anthracnose and its application |
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| CN101209062A (en) * | 2006-12-30 | 2008-07-02 | 中国医学科学院药用植物研究所 | Compound plant source agricultural fungicide and preparation thereof |
| CN102369956A (en) * | 2011-10-18 | 2012-03-14 | 浙江农林大学 | Plant essential oil containing agricultural fungicide and sterilization applications thereof |
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