CN103380789B - Natural bactericide for preventing and curing pear scab and preparation method thereof - Google Patents
Natural bactericide for preventing and curing pear scab and preparation method thereof Download PDFInfo
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- CN103380789B CN103380789B CN201310270687.6A CN201310270687A CN103380789B CN 103380789 B CN103380789 B CN 103380789B CN 201310270687 A CN201310270687 A CN 201310270687A CN 103380789 B CN103380789 B CN 103380789B
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Abstract
The invention provides a natural bactericide for preventing and curing pear scab. The natural bactericide is composed of following components: dryopteris crasirhitom nakai, tarragon, hairyvein agrimony, betel nut, coptis, alchornea davidii, Chinese gall, prunella vulgaris, cordate houttuynia and garlic. The natural bactericide for preventing and curing pear scab has advantages of wide raw material source and low cost, is composed of pure natural components, not only is non-toxic and non-harmful, but also can effectively prevent and cure pear scab.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent preventing and treating pear scab and preparation method thereof.
Background technology
Pear scab is one of Major Diseases of pear tree.Pear scab is mainly caused harm Pears, leaf stalk, fruit, carpopodium, bud and young sprout.Site of pathological change produces black mustiness thing, and this is the principal character of this disease.Scab is circular, faint yellow, produces black mould above rear, and then suberification gradually, depression be full of cracks, deformed fruit produces depression spot, upper long black mould.After blade is injured, first along vein or offshoot, circular or not whole rule shape scab occur at leaf back, produce the mould layer of black, when falling ill serious, blade spreads all over black mould, and scab place, leaf front chlorisis turns yellow and causes leaf abscission; After young sprout is injured, how in base portion morbidity, scab subcircular or oblong, the mould layer of upper long black, later stage scab depression, periphery or face checking are scab shape.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for preventing fungi normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control pear scab that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating pear scab provided by the invention, is made up of following component: rhizome of cyrtomium, tarragon, hairyvein agrimony, betel nut, the coptis, mountain waste of flax, gall nut, selfheal, cordate houttuynia, garlic.
Be made up of the component of following weight portion: rhizome of cyrtomium 4-6 part, tarragon 4-6 part, hairyvein agrimony 4-6 part, betel nut 4-6 part, coptis 2-4 part, mountain waste of flax 1-3 part, gall nut 1-3 part, selfheal 1-3 part, cordate houttuynia 0.5-1.5 part, garlic 1-3 part.
Be made up of the component of following weight portion: rhizome of cyrtomium 5 parts, tarragon 5 parts, hairyvein agrimony 5 parts, betel nut 5 parts, the coptis 3 parts, mountain waste of flax 2 parts, gall nut 2 parts, selfheal 2 parts, cordate houttuynia 1 part, 2 parts, garlic.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control pear scab, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is rhizome of cyrtomium, tarragon, hairyvein agrimony, betel nut, the coptis, mountain waste of flax, gall nut, selfheal, cordate houttuynia and garlic;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of pear scab.
Present invention also offers the application of natural bactericidal agent in control pear scab of above-mentioned control pear scab, be specially: the natural bactericidal agent of control pear scab is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control pear scab provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and effectively can prevent and treat pear scab.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control pear scab, is made up of the component of following weight portion: rhizome of cyrtomium 4 parts, tarragon 5 parts, hairyvein agrimony 4 parts, betel nut 5 parts, the coptis 2 parts, mountain waste of flax 1 part, gall nut 3 parts, selfheal 3 parts, cordate houttuynia 1.5 parts, 2 parts, garlic.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is rhizome of cyrtomium, tarragon, hairyvein agrimony, betel nut, the coptis, mountain waste of flax, gall nut, selfheal, cordate houttuynia and garlic;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of pear scab.
Embodiment 2
The natural bactericidal agent of control pear scab, is made up of the component of following weight portion: rhizome of cyrtomium 6 parts, tarragon 6 parts, hairyvein agrimony 6 parts, betel nut 4 parts, the coptis 4 parts, mountain waste of flax 3 parts, gall nut 1 part, selfheal 1 part, cordate houttuynia 0.5 part, 1 part, garlic.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is rhizome of cyrtomium, tarragon, hairyvein agrimony, betel nut, the coptis, mountain waste of flax, gall nut, selfheal, cordate houttuynia and garlic;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 80 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 80 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of pear scab.
Embodiment 3
The natural bactericidal agent of control pear scab, is made up of the component of following weight portion: rhizome of cyrtomium 5 parts, tarragon 4 parts, hairyvein agrimony 5 parts, betel nut 6 parts, the coptis 3 parts, mountain waste of flax 2 parts, gall nut 2 parts, selfheal 2 parts, cordate houttuynia 1 part, 3 parts, garlic.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is rhizome of cyrtomium, tarragon, hairyvein agrimony, betel nut, the coptis, mountain waste of flax, gall nut, selfheal, cordate houttuynia and garlic;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of pear scab.
Embodiment 4 measures the natural bactericidal agent of the control pear scab of embodiment 1 to 3 to the inhibit activities of strawberry pathogen
This experiment, in vitro, measures the natural bactericidal agent of control pear scab of embodiment 1 to 3 to the inhibit activities of strawberry pathogen, method and result as follows:
1. for examination bacterial classification
Pears black star spore (Fusicladium nashico-la Tanaka et Yamanoto) is commercially available
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of above-mentioned germ, by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment pear scab of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3
Claims (4)
1. prevent and treat a natural bactericidal agent for pear scab, it is characterized in that: be made up of the component of following weight portion: rhizome of cyrtomium 4-6 part, tarragon 4-6 part, hairyvein agrimony 4-6 part, betel nut 4-6 part, coptis 2-4 part, mountain waste of flax 1-3 part, gall nut 1-3 part, selfheal 1-3 part, cordate houttuynia 0.5-1.5 part, garlic 1-3 part;
The preparation method of this bactericide, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is rhizome of cyrtomium, tarragon, hairyvein agrimony, betel nut, the coptis, mountain waste of flax, gall nut, selfheal, cordate houttuynia and garlic;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of pear scab.
2. a kind of natural bactericidal agent preventing and treating pear scab according to claim 1, is characterized in that: be made up of the component of following weight portion: rhizome of cyrtomium 5 parts, tarragon 5 parts, hairyvein agrimony 5 parts, betel nut 5 parts, the coptis 3 parts, mountain waste of flax 2 parts, gall nut 2 parts, selfheal 2 parts, cordate houttuynia 1 part, 2 parts, garlic.
3. a preparation method for the natural bactericidal agent of the control pear scab described in claim 1 or 2, is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is rhizome of cyrtomium, tarragon, hairyvein agrimony, betel nut, the coptis, mountain waste of flax, gall nut, selfheal, cordate houttuynia and garlic;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of pear scab.
4. the application of natural bactericidal agent in control pear scab of the control pear scab described in claim 1 or 2, is characterized in that: mixed with 500-1000 times of water gaging by the natural bactericidal agent of control pear scab and be sterilization microbial inoculum.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872201A (en) * | 2015-06-03 | 2015-09-02 | 张亚鹏 | Composition for preventing and curing scab of apple trees and preparation method thereof |
| CN104938536A (en) * | 2015-06-04 | 2015-09-30 | 胡凡营 | Plant-derived biopesticide and preparation method thereof |
| CN105053075A (en) * | 2015-07-20 | 2015-11-18 | 句容市后白镇解氏家庭农场 | Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide |
| CN105394102A (en) * | 2015-12-05 | 2016-03-16 | 赵兰 | Pollution-free bactericide for preventing and treating root rot of Chinese strawberry and preparation method of pollution-free bactericide |
| CN105519599A (en) * | 2015-12-24 | 2016-04-27 | 青岛百瑞吉生物工程有限公司 | Natural bactericide for preventing and treating angular leaf spot of persimmon trees, and preparation method thereof |
| CN105851066B (en) * | 2016-05-04 | 2018-07-13 | 北京联合大学 | A kind of natural bactericidal agent and preparation method thereof of prevention peach scab |
| CN107409891A (en) * | 2017-04-01 | 2017-12-01 | 阜南县鑫荣种养专业合作社 | A kind of implantation methods for preventing and treating pear scab |
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| CN101107934A (en) * | 2006-07-17 | 2008-01-23 | 河北农业大学 | Fungicide containing nutgall extractive and composition thereof |
| CN102919287A (en) * | 2012-08-27 | 2013-02-13 | 张清明 | Mancozeb Chinese herbal medicine complex bactericide used for controlling paddy rice sheath blight |
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