CN104041538B - A kind ofly prevent and treat natural bactericidal agent of strawberry black spot and preparation method thereof - Google Patents
A kind ofly prevent and treat natural bactericidal agent of strawberry black spot and preparation method thereof Download PDFInfo
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- CN104041538B CN104041538B CN201410229167.5A CN201410229167A CN104041538B CN 104041538 B CN104041538 B CN 104041538B CN 201410229167 A CN201410229167 A CN 201410229167A CN 104041538 B CN104041538 B CN 104041538B
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Abstract
A kind of natural bactericidal agent preventing and treating strawberry black spot provided by the invention, is made up of following component: garlic, peppermint, lotus leaf, schizonepeta, myrobalan, tarragon, cassia bark, dogwood, lopseed, myrrh.The natural bactericidal agent abundant raw material source of this control strawberry black spot, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat strawberry black spot.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind ofly prevent and treat natural bactericidal agent of strawberry black spot and preparation method thereof.
Background technology
Strawberry black spot is a kind of important disease on strawberry, and in China, strawberry production Area distribution is commonplace.Strawberry Alternaria alternata caused occurrence is Deuteromycotina, Alternariaalternata (Fries) Keissler of Alternaria.Alternaria fragriae with mycelium etc. on plant or land tissue and survive the winter.Propagate by means of seedling etc., the germ spore in environment also can cause and infects morbidity.Hot and humid weather is conducive to infecting of black spot and spreads, and field microclimate humidity is conducive to morbidity.The morbidity of continuous cropping field increases the weight of.Between strawberry cultivars, resistance is different.
Strawberry black spot mainly causes harm blade, petiole, stem and berry.It is generally the unsetting scab of black producing diameter 5-8 millimeters on blade face that blade is fallen ill, and slightly in wheel line shape, scab central authorities are in taupe, and have the mould layer of arachnoid, scab often has yellow halo outward.On petiole and stolon, morbidity is normal in the little pit of brown, and when scab was around one week, handle or stem due to illness portion's contracting of hanging dry up frangibility.Fruit pastes ground fruit catches an illness more, and the scab on berry is black, and above have the mould layer of grey black cigarette ash shape, scab, only at cortex, does not generally go deep into pulp, but because of the pollution of black mould layer, and make berry lose commodity value.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for control strawberry black spot is normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control strawberry black spot that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating strawberry black spot provided by the invention, is made up of following component: garlic, peppermint, lotus leaf, schizonepeta, myrobalan, tarragon, cassia bark, dogwood, lopseed, myrrh.
Preferably, be made up of the component of following weight portion: garlic 10-20 part, peppermint 5-15 part, lotus leaf 8-12 part, schizonepeta 8-12 part, myrobalan 4-6 part, tarragon 4-6 part, cassia bark 2-4 part, dogwood 2-4 part, lopseed 1-3 part, myrrh 1-3 part.
More preferably, be made up of the component of following weight portion: 15 parts, garlic, peppermint 10 parts, 10 parts, lotus leaf, schizonepeta 10 parts, myrobalan 5 parts, tarragon 5 parts, 3 parts, cassia bark, dogwood 3 parts, lopseed 2 parts, myrrh 2 parts.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control strawberry black spot, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is garlic, peppermint, lotus leaf, schizonepeta, myrobalan, tarragon, cassia bark, dogwood, lopseed and myrrh;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry black spot.
Present invention also offers the application of the natural bactericidal agent of above-mentioned control strawberry black spot, be specially: the natural bactericidal agent of control strawberry black spot is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control strawberry black spot provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat strawberry black spot.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control strawberry black spot, is made up of the component of following weight portion: 15 parts, garlic, peppermint 10 parts, 10 parts, lotus leaf, schizonepeta 10 parts, myrobalan 5 parts, tarragon 5 parts, 3 parts, cassia bark, dogwood 3 parts, lopseed 2 parts, myrrh 2 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is garlic, peppermint, lotus leaf, schizonepeta, myrobalan, tarragon, cassia bark, dogwood, lopseed and myrrh;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry black spot.
Embodiment 2
The natural bactericidal agent of control strawberry black spot, is made up of the component of following weight portion: 20 parts, garlic, peppermint 5 parts, 8 parts, lotus leaf, schizonepeta 12 parts, myrobalan 4 parts, tarragon 4 parts, 4 parts, cassia bark, dogwood 4 parts, lopseed 3 parts, myrrh 1 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is garlic, peppermint, lotus leaf, schizonepeta, myrobalan, tarragon, cassia bark, dogwood, lopseed and myrrh;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 70 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry black spot.
Embodiment 3
The natural bactericidal agent of control strawberry black spot, is made up of the component of following weight portion: 10 parts, garlic, peppermint 15 parts, 12 parts, lotus leaf, schizonepeta 8 parts, myrobalan 6 parts, tarragon 6 parts, 2 parts, cassia bark, dogwood 2 parts, lopseed 1 part, myrrh 3 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is garlic, peppermint, lotus leaf, schizonepeta, myrobalan, tarragon, cassia bark, dogwood, lopseed and myrrh;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry black spot.
Embodiment 4 measures the natural bactericidal agent of the control strawberry black spot of embodiment 1 to 3 to the inhibit activities of strawberry pathogen
This experiment, in vitro, measures the natural bactericidal agent of control strawberry black spot of embodiment 1 to 3 to the inhibit activities of strawberry pathogen, method and result as follows:
1. for examination bacterial classification
Alternaria Alternariaalternate (Fries) Keissler is commercially available.
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of Alternaria Alternariaalternate (Fries) Keissler, by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment strawberry black spot of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry black spot of table 3 embodiment 1 to 3
Claims (4)
1. prevent and treat a natural bactericidal agent for strawberry black spot, it is characterized in that: be made up of the component of following weight portion: garlic 10-20 part, peppermint 5-15 part, lotus leaf 8-12 part, schizonepeta 8-12 part, myrobalan 4-6 part, tarragon 4-6 part, cassia bark 2-4 part, dogwood 2-4 part, lopseed 1-3 part, myrrh 1-3 part.
2. a kind of natural bactericidal agent preventing and treating strawberry black spot according to claim 1, is characterized in that: be made up of the component of following weight portion: 15 parts, garlic, peppermint 10 parts, 10 parts, lotus leaf, schizonepeta 10 parts, myrobalan 5 parts, tarragon 5 parts, 3 parts, cassia bark, dogwood 3 parts, lopseed 2 parts, myrrh 2 parts.
3. a kind of preparation method preventing and treating the natural bactericidal agent of strawberry black spot according to claim 1, is characterized in that: comprise the following steps:
By raw material mixing after pulverizing, sieving respectively, obtain compound; Described raw material is garlic, peppermint, lotus leaf, schizonepeta, myrobalan, tarragon, cassia bark, dogwood, lopseed and myrrh;
Compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
Filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
Filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
Filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry black spot.
4. the application of natural bactericidal agent in control strawberry black spot of control strawberry black spot according to claim 1, is characterized in that: mixed with 500-1000 times of water gaging by the natural bactericidal agent of control strawberry black spot and be sterilization microbial inoculum.
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| CN104814056B (en) * | 2015-04-17 | 2018-01-19 | 常州汇森电子有限公司 | A kind of method for preventing and treating apricot black spot |
| CN105053075A (en) * | 2015-07-20 | 2015-11-18 | 句容市后白镇解氏家庭农场 | Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide |
| CN105850476A (en) * | 2016-04-28 | 2016-08-17 | 郎溪县建平镇荣香瓜果种植家庭农场 | Method for preventing and treating conventional diseases of strawberries |
| CN105994451A (en) * | 2016-07-29 | 2016-10-12 | 广西素安生态农业有限公司 | Chinese herbal medicine control agent for common diseases of strawberries and preparation method thereof |
| CN106631357A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Fertilizer capable of preventing and controlling black spot of stevia rebaudiana |
| CN106577889A (en) * | 2016-11-14 | 2017-04-26 | 广西素安生态农业有限公司 | Chinese herbal medicinal preventing and treating agent for phytophthora blight of pepper and preparation method thereof |
| CN106942295A (en) * | 2017-05-03 | 2017-07-14 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating safron black spot |
| CN106962419A (en) * | 2017-05-03 | 2017-07-21 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating safron black spot and its application |
| CN106889121A (en) * | 2017-05-03 | 2017-06-27 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew and its application |
| CN107027819A (en) * | 2017-05-03 | 2017-08-11 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew |
| US11166466B2 (en) * | 2019-04-11 | 2021-11-09 | Jh Biotech Inc. | Biological fungicide composed by plant extracts |
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