CN103461401B - A kind ofly prevent and treat natural bactericidal agent of ginkgo dead leaf disease and preparation method thereof - Google Patents
A kind ofly prevent and treat natural bactericidal agent of ginkgo dead leaf disease and preparation method thereof Download PDFInfo
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Abstract
A kind of natural bactericidal agent preventing and treating ginkgo dead leaf disease provided by the invention, makes: pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly, composite polymineral by following component.The natural bactericidal agent abundant raw material source of this control ginkgo dead leaf disease, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and it is sick effectively can to prevent and treat ginkgo dead leaf.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind ofly prevent and treat natural bactericidal agent of ginkgo dead leaf disease and preparation method thereof.
Background technology
Ginkgo dead leaf disease is one of Major Diseases of ginkgo, and in Guangxi, Zhejiang, Jiangsu, the ginkgo such as Shandong concentrates producing region to have generation in various degree.The newer producing region morbidity of general old producing region is serious.Susceptible plant, the lighter's partial blade is withered in advance to come off, and severe one blade is de-light all, thus cause tree vigo(u)r weak, retarded growth.The sick initial stage common blade tip of ginkgo dead leaf turns yellow, and between June, browning is withered gradually for yellow spot, and by extending partially into whole leaf margin, in brown to leaf margin scab russet.Thereafter, scab spreads to blade base gradually, until whole blade overstrike or taupe, dried-up come off till.7-August scab and the boundary of health tissues obvious, scab edge be corrugated, and comparatively deeply, its peripheral edge portion is also shown in narrower or wider foresythia tape to color.September rises, and scab obviously increases, and uneven phenomenon appears in diffusion edge, and sick strong boundary of organization is also gradually not obvious.In addition on indefinite position, some irregular faded spots are produced, center brown, though these spot wood obviously expand, often with the leaf margin spot phase Colaesce extended at the blade of nursery stock or large tree base portion coppice shoot 9 ~ October.The producing cause of ginkgo seedling damping off it has been generally acknowledged that and caused by rod method alternariaalternata (fr.) Keissl, GLOMERFLLA CINGULATA glomereltacingulate (Stonem) Spauld.etSchrenk and Pestalotiaginkgo (Pestalotia ginkgo hori) infection, not easily prevention and control.
Be chemical pesticide bactericide for control ginkgo dead leaf is sick normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, make various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control ginkgo dead leaf disease that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating ginkgo dead leaf disease provided by the invention, makes: pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly, composite polymineral by following component.
Preferably: pig label grass 4-6 part, sealwort 4-6 part, granatum 4-6 part, elscholtiza 4-6 part, coptis 2-4 part, eupatorium 1-3 part, peppermint 1-3 part, fruit of Cherokee rose 1-3 part, Chinese holly 0.5-1.5 part, composite polymineral 1-3 part is made by the component of following weight portion.
More preferably: pig label grass 5 parts, sealwort 5 parts, granatum 5 parts, elscholtiza 5 parts, the coptis 3 parts, eupatorium 2 parts, peppermint 2 parts, the fruit of Cherokee rose 2 parts, Chinese holly 1 part, composite polymineral 2 parts is made by the component of following weight portion.
Wherein, described composite polymineral comprises boron, zinc, molybdenum, manganese, copper, iron, and per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams, surplus is the elements such as carbon, oxygen, nitrogen, phosphorus.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control ginkgo dead leaf disease, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
Present invention also offers the application of natural bactericidal agent in control ginkgo dead leaf disease of above-mentioned control ginkgo dead leaf disease, be specially: the natural bactericidal agent of control ginkgo dead leaf disease is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control ginkgo dead leaf disease provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and it is sick effectively can to prevent and treat ginkgo dead leaf.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control ginkgo dead leaf disease, makes: pig label grass 4 parts, sealwort 5 parts, granatum 4 parts, elscholtiza 5 parts, the coptis 2 parts, eupatorium 1 part, peppermint 3 parts, the fruit of Cherokee rose 3 parts, Chinese holly 1.5 parts, composite polymineral 2 parts by the component of following weight portion.Per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
Embodiment 2
The natural bactericidal agent of control ginkgo dead leaf disease, makes: pig label grass 6 parts, sealwort 6 parts, granatum 6 parts, elscholtiza 4 parts, the coptis 4 parts, eupatorium 3 parts, peppermint 1 part, the fruit of Cherokee rose 1 part, Chinese holly 0.5 part, composite polymineral 1 part by the component of following weight portion.Per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 80 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 80 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
Embodiment 3
The natural bactericidal agent of control ginkgo dead leaf disease, makes: pig label grass 5 parts, sealwort 4 parts, granatum 5 parts, elscholtiza 6 parts, the coptis 3 parts, eupatorium 2 parts, peppermint 2 parts, the fruit of Cherokee rose 2 parts, Chinese holly 1 part, composite polymineral 3 parts by the component of following weight portion.Per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
Embodiment 4 measures the natural bactericidal agent of the control ginkgo dead leaf disease of embodiment 1 to 3 to the inhibit activities of strawberry pathogen
This experiment, in vitro, measures the natural bactericidal agent of control ginkgo dead leaf disease of embodiment 1 to 3 to the inhibit activities of strawberry pathogen, method and result as follows:
1. for examination bacterial classification
Rod method alternariaalternata (fr.) Keissl is commercially available
GLOMERFLLA CINGULATA glomereltacingulate (Stonem) Spauld.etSchrenk is commercially available
Pestalotiaginkgo (Pestalotia ginkgo hori) is commercially available
2.EC50 measures
Utilize mycelial growth rate method and spore germination method to measure embodiment of the present invention 1-3 natural bactericidal agent to the EC50 value of above-mentioned germ, the results are shown in Table 1.
Table 1 embodiment 1 natural bactericidal agent is to the EC50 value for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500 and 1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment ginkgo dead leaf of embodiment 1 to 3 sick, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3
Claims (4)
1. prevent and treat a natural bactericidal agent for ginkgo dead leaf disease, it is characterized in that: be made up of the component of following weight portion: pig label grass 4-6 part, sealwort 4-6 part, granatum 4-6 part, elscholtiza 4-6 part, coptis 2-4 part, eupatorium 1-3 part, peppermint 1-3 part, fruit of Cherokee rose 1-3 part, Chinese holly 0.5-1.5 part, composite polymineral 1-3 part; Described composite polymineral comprises boron, zinc, molybdenum, manganese, copper, iron, and per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams.
2. a kind of natural bactericidal agent preventing and treating ginkgo dead leaf disease according to claim 1, is characterized in that: make: pig label grass 5 parts, sealwort 5 parts, granatum 5 parts, elscholtiza 5 parts, the coptis 3 parts, eupatorium 2 parts, peppermint 2 parts, the fruit of Cherokee rose 2 parts, Chinese holly 1 part, composite polymineral 2 parts by the component of following weight portion.
3. a preparation method for the natural bactericidal agent of the control ginkgo dead leaf disease described in claim 1 or 2, is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
4. the application of natural bactericidal agent in control ginkgo dead leaf disease of the control ginkgo dead leaf disease described in claim 1 or 2, is characterized in that: mixed with 500-1000 times of water gaging by the natural bactericidal agent of control ginkgo dead leaf disease and be sterilization microbial inoculum.
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| CN104996489A (en) * | 2015-06-03 | 2015-10-28 | 广西大学 | Natural bactericide for preventing and controlling pepper anthracnose |
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| CN107624821A (en) * | 2017-09-27 | 2018-01-26 | 贵州蓝天生态农业科技发展有限公司 | A kind of composition for treating Momordica grosvenori southern blight |
| CN108551961A (en) * | 2018-05-15 | 2018-09-21 | 务川县文娟农业开发有限公司 | A method of prevention podocarpus dead leaf disease |
| CN112174728A (en) * | 2020-09-27 | 2021-01-05 | 中科美兰（合肥）生物工程有限公司 | Biological source multifunctional water-soluble pesticide fertilizer special for ginkgo leaf blight |
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