CN104996492A - Natural bactericidal agent for preventing and controlling loquat canker, and preparation method thereof - Google Patents
Natural bactericidal agent for preventing and controlling loquat canker, and preparation method thereof Download PDFInfo
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- CN104996492A CN104996492A CN201510428256.7A CN201510428256A CN104996492A CN 104996492 A CN104996492 A CN 104996492A CN 201510428256 A CN201510428256 A CN 201510428256A CN 104996492 A CN104996492 A CN 104996492A
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- loquat
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Abstract
The present invention provides a natural bactericidal agent for preventing and controlling loquat canker, wherein the natural bactericidal agent is prepared from the following components: euphorbia esula l, garlic, lobelia chinensis lour, rhizoma arisaematis, folium hibisci mutabilis, polygonum cuspidatum, catharanthus roseus, flos magnoliae, radix isatidis and coptis chinensis. According to the present invention, the natural bactericidal agent has characteristics of rich raw material source and low cost, and is made from the pure natural ingredients so as to have the characteristics of no toxicity and no pollution and further effectively prevent and control the loquat canker.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind ofly prevent and treat natural bactericidal agent of loquat canker and preparation method thereof.
Background technology
Loquat, another name: reed tangerine, golden ball, Lu Zhi, the rose family, Eriobotrya, loquat originates in southeast China, and because leaf-shaped is like Chinese lute musical instrument and name, its flower can be used as medicine.The height of tree 3 ~ 5 meters, leaf is large and grow, thick and have fine hair, and in oblong, shape is as Chinese lute.Another name is again reed tangerine, Lu Zhi, golden ball, scorching fruit, Jiao Zi.Loquat is different from most of fruit tree, and in autumn or bloom early winter, fruit is ripe in spring to early summer, all more Zao than other fruit, is therefore " solely for seasonal pathogens,exopathogens person in fruit tree " by title.The flower of loquat is white or faint yellow, has five pieces of petals, diameter about 2 centimetres, a branch of with five to ten one-tenth, can as nectar source crop.
Loquat canker, also known as bud blight, is the disease that a kind of harmfulness is very large, should strictly quarantines, prevent the spreading and propagation of disease.Infection process young sprout often causes bud withered.Limb is injured and is often occurred cancerous swelling symptom, often becomes the nest room of insect at cancerous swelling position.After bough is susceptible, often cause withered, make tree body weak, output reduces, and even complete stool is dead.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for preventing fungi normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control loquat canker that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating loquat canker provided by the invention, is made up of following component: euphorbia lunulata, garlic, Chinese lobelia, rhizoma arisaematis, cottonrose hibiscus leaf, giant knotweed, catharanthus roseus, the flower bud of lily magnolia, Radix Isatidis, the coptis.
Be made up of the component of following weight portion: euphorbia lunulata 8-12 part, garlic 4-6 part, Chinese lobelia 4-6 part, rhizoma arisaematis 2-4 part, cottonrose hibiscus leaf 2-4 part, giant knotweed 1-3 part, catharanthus roseus 1-3 part, flower bud of lily magnolia 1-3 part, Radix Isatidis 0.5-1.5 part, coptis 0.5-1.5 part.
Be made up of the component of following weight portion: euphorbia lunulata 10 parts, 5 parts, garlic, Chinese lobelia 5 parts, rhizoma arisaematis 3 parts, cottonrose hibiscus leaf 3 parts, giant knotweed 2 parts, catharanthus roseus 2 parts, the flower bud of lily magnolia 2 parts, Radix Isatidis 1 part, the coptis 1 part.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control loquat canker, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is euphorbia lunulata, garlic, Chinese lobelia, rhizoma arisaematis, cottonrose hibiscus leaf, giant knotweed, catharanthus roseus, the flower bud of lily magnolia, Radix Isatidis and the coptis;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of loquat canker.
Present invention also offers the application of the natural bactericidal agent of above-mentioned control loquat canker, be specially: the natural bactericidal agent of control loquat canker is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control loquat canker provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat loquat canker.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control loquat canker, is made up of the component of following weight portion: euphorbia lunulata 10 parts, 5 parts, garlic, Chinese lobelia 4 parts, rhizoma arisaematis 2 parts, cottonrose hibiscus leaf 2 parts, giant knotweed 1 part, catharanthus roseus 3 parts, the flower bud of lily magnolia 3 parts, Radix Isatidis 1.5 parts, the coptis 0.5 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is euphorbia lunulata, garlic, Chinese lobelia, rhizoma arisaematis, cottonrose hibiscus leaf, giant knotweed, catharanthus roseus, the flower bud of lily magnolia, Radix Isatidis and the coptis;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of loquat canker.
Embodiment 2
The natural bactericidal agent of control loquat canker, is made up of the component of following weight portion: euphorbia lunulata 8 parts, 6 parts, garlic, Chinese lobelia 6 parts, rhizoma arisaematis 4 parts, cottonrose hibiscus leaf 4 parts, giant knotweed 3 parts, catharanthus roseus 1 part, the flower bud of lily magnolia 1 part, Radix Isatidis 0.5 part, the coptis 1.5 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is euphorbia lunulata, garlic, Chinese lobelia, rhizoma arisaematis, cottonrose hibiscus leaf, giant knotweed, catharanthus roseus, the flower bud of lily magnolia, Radix Isatidis and the coptis;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 70 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of loquat canker.
Embodiment 3
The natural bactericidal agent of control loquat canker, is made up of the component of following weight portion: euphorbia lunulata 12 parts, 4 parts, garlic, Chinese lobelia 5 parts, rhizoma arisaematis 3 parts, cottonrose hibiscus leaf 3 parts, giant knotweed 2 parts, catharanthus roseus 2 parts, the flower bud of lily magnolia 2 parts, Radix Isatidis 1 part, the coptis 1 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is euphorbia lunulata, garlic, Chinese lobelia, rhizoma arisaematis, cottonrose hibiscus leaf, giant knotweed, catharanthus roseus, the flower bud of lily magnolia, Radix Isatidis and the coptis;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of loquat canker.
Embodiment 4 measures the natural bactericidal agent of the control loquat canker of embodiment 1 to 3 to the inhibit activities of loquat pathogen
This experiment, in vitro, measures the natural bactericidal agent of control loquat canker of embodiment 1 to 3 to the inhibit activities of loquat pathogen, method and result as follows:
1. for examination bacterial classification
Loquat gray mold Pseudomonassyringfaepv.eriobotryae (Takimoto) YoungDyeWilkie.11215 is commercially available.
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of tail spore bacterium Cercospora insulana Sacc, by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment loquat canker of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment loquat canker of table 3 embodiment 1 to 3
Claims (5)
1. prevent and treat a natural bactericidal agent for loquat canker, it is characterized in that: be made up of following component: euphorbia lunulata, garlic, Chinese lobelia, rhizoma arisaematis, cottonrose hibiscus leaf, giant knotweed, catharanthus roseus, the flower bud of lily magnolia, Radix Isatidis, the coptis.
2. a kind of natural bactericidal agent preventing and treating loquat canker according to claim 1, is characterized in that: be made up of the component of following weight portion: euphorbia lunulata 8-12 part, garlic 4-6 part, Chinese lobelia 4-6 part, rhizoma arisaematis 2-4 part, cottonrose hibiscus leaf 2-4 part, giant knotweed 1-3 part, catharanthus roseus 1-3 part, flower bud of lily magnolia 1-3 part, Radix Isatidis 0.5-1.5 part, coptis 0.5-1.5 part.
3. a kind of natural bactericidal agent preventing and treating loquat canker according to claim 1, is characterized in that: be made up of the component of following weight portion: euphorbia lunulata 10 parts, 5 parts, garlic, Chinese lobelia 5 parts, rhizoma arisaematis 3 parts, cottonrose hibiscus leaf 3 parts, giant knotweed 2 parts, catharanthus roseus 2 parts, the flower bud of lily magnolia 2 parts, Radix Isatidis 1 part, the coptis 1 part.
4. prevent and treat a preparation method for the natural bactericidal agent of loquat canker, it is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is euphorbia lunulata, garlic, Chinese lobelia, rhizoma arisaematis, cottonrose hibiscus leaf, giant knotweed, catharanthus roseus, the flower bud of lily magnolia, Radix Isatidis and the coptis;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of loquat canker.
5. prevent and treat the application of natural bactericidal agent in control loquat canker of loquat canker, it is characterized in that: the natural bactericidal agent of control loquat canker is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
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| CN201510428256.7A CN104996492A (en) | 2015-07-20 | 2015-07-20 | Natural bactericidal agent for preventing and controlling loquat canker, and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105875293A (en) * | 2016-06-07 | 2016-08-24 | 青川县宏宇猕猴桃种植专业合作社 | Kiwi fruit canker prevention method and prevention agent |
| CN107751264A (en) * | 2017-09-15 | 2018-03-06 | 海门市利美农副产品有限公司 | A kind of Farm chemical of Chinese berbs and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105875293A (en) * | 2016-06-07 | 2016-08-24 | 青川县宏宇猕猴桃种植专业合作社 | Kiwi fruit canker prevention method and prevention agent |
| CN107751264A (en) * | 2017-09-15 | 2018-03-06 | 海门市利美农副产品有限公司 | A kind of Farm chemical of Chinese berbs and preparation method thereof |
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Application publication date: 20151028 |