CN103380787A - Natural bactericide for preventing and curing nursery stock damping-off and preparation method thereof - Google Patents
Natural bactericide for preventing and curing nursery stock damping-off and preparation method thereof Download PDFInfo
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- CN103380787A CN103380787A CN2013102731651A CN201310273165A CN103380787A CN 103380787 A CN103380787 A CN 103380787A CN 2013102731651 A CN2013102731651 A CN 2013102731651A CN 201310273165 A CN201310273165 A CN 201310273165A CN 103380787 A CN103380787 A CN 103380787A
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Abstract
The invention provides a natural bactericide for preventing and curing nursery stock damping-off. The natural bactericide is composed of following components: mulberry leaves, tarragon, celandine, acalypha australis, coptis, buddhas lamp twigs and leaves, pogostemon, cirsium japonicum, aconitum kusnezoffii, and cordate houttuynia. The natural bactericide for preventing and curing nursery stock damping-off has the advantages of wide raw material resources and low cost, is composed of pure natural components, not only is non-toxic and non-harmful, but also can effectively prevent and cure the nursery stock damping-off.
Description
Technical field
The invention belongs to the prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent of preventing and treating the nursery stock damping off and preparation method thereof.
Background technology
The nursery stock damping off is commonly called as " seedling ", " mould ", " bound feet pest ", is the most serious the most general disease that occurs in Gardening Nursery, mainly betides the Seedling Stage of forest flower plant.Mainly by due to melon and fruit pythium Mastigomycotina fungal infection.Rotten mould and some kinds Phytophthora of thorn also can cause morbidity.The germ host range is very wide, can cause in flakes dead seedling when serious.Do not contaminated when seed or young shoot are unearthed and rot, in the Seedling Stage morbidity, the basal part of stem under earth's surface or earth's surface presents water stain shape scab, sick section yellowish-brown, and the contracting of hanging can to plant top and the bottom expansion, be wire.Patient's condition development is organized disintegration rapidly, the young stem lodging of namely wilting, but limb is green in a short time, if during humid environment, sick section and near native face also can to grow white wool shape mould.
At present, in the plantation of vegetables, melon and fruit, for prevent fungi normally used be the chemical pesticide bactericide, because a large amount of problem of environmental pollutions that use chemical pesticides to cause are on the rise.Simultaneously, adopt the chemical pesticide bactericide, the time various phytopathogen bacterial classifications produce drug resistances, pesticide resistance, increased the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, can threaten to useful biology yet.And a lot of chemical pesticides are non-degradables or are difficult to degrade, and easily pollute the enrichment of thing at the animals and plants cylinder accumulation.
Along with the public is more and more higher to the requirement of " pollution-free food ", " pollution-free food ", but also increase gradually to the ecological agricultural chemical demand of pollution-free natural degradation in market.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals, be the inexorable trend of society and Development of Natural Science, so the new bio wide-spectrum bactericide has wide development prospect on pesticide manufacturing uses.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the purpose of this invention is to provide a kind of environmental protection, control nursery stock damping off that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent of preventing and treating the nursery stock damping off provided by the invention, made by following component: mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis, cordate houttuynia.
Component by following weight portion is made: mulberry leaf 4-6 part, tarragon 4-6 part, greater celandine 4-6 part, acalypha copperleaf 4-6 part, coptis 3-5 part, Dong's sauce back of the body 1-3 part, Pogostemon cablin 1-3 part, setose thistle 1-3 part, radix aconiti agrestis 0.5-1.5 part, cordate houttuynia 1-3 part.
Component by following weight portion is made: 5 parts, mulberry leaf, 5 parts of tarragons, 5 parts of greater celandines, 5 parts of acalypha copperleafs, 4 parts of the coptiss, 2 parts of Dong's sauce back ofs the body, 2 parts of Pogostemon cablin, 2 parts of setose thistles, 1 part of radix aconiti agrestis, 2 parts of cordate houttuynias.
The present invention also provides the preparation method of the natural bactericidal agent of above-mentioned control nursery stock damping off, comprises the following steps:
(1) with raw material mixing after pulverizing, sieving respectively, get compound; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 60-80 ℃ and decoct 1-3h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 60-80 ℃ and decocts 1-3h;
(4) filter, merging filtrate is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control nursery stock damping off.
The application of the natural bactericidal agent that the present invention also provides above-mentioned control nursery stock damping off in control nursery stock damping off is specially: the natural bactericidal agent that will prevent and treat the nursery stock damping off mixes with 500-1000 times of water gaging and is the sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent raw material sources of control nursery stock damping off provided by the invention are abundant, and are with low cost, adopt the component of pure natural to make, Nonpoisonous, non-environmental-pollution not only, and can effectively prevent and treat the nursery stock damping off.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described concrete material proportion of embodiment, process conditions and result thereof only are used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1
The natural bactericidal agent of control nursery stock damping off is made by the component of following weight portion: 4 parts, mulberry leaf, 5 parts of tarragons, 4 parts of greater celandines, 5 parts of acalypha copperleafs, 4 parts of the coptiss, 1 part of Dong's sauce back of the body, 3 parts of Pogostemon cablin, 3 parts of setose thistles, 1.5 parts of radix aconiti agrestis, 2 parts of cordate houttuynias.
Its preparation method comprises the following steps:
(1) with raw material mixing after pulverizing, sieving respectively, get compound; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 8 times of amounts 95%, be heated to 70 ℃ and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times of amounts 95%, is heated to 60 ℃ and decocts 1-3h;
(4) filter, merging filtrate is concentrated into ethanol and adds 1/15 of total amount, adds the water of 2 times of volumes of concentrate, standing 36h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control nursery stock damping off.
Embodiment 2
The natural bactericidal agent of control nursery stock damping off is made by the component of following weight portion: 6 parts, mulberry leaf, 6 parts of tarragons, 6 parts of greater celandines, 4 parts of acalypha copperleafs, 3 parts of the coptiss, 3 parts of Dong's sauce back ofs the body, 1 part of Pogostemon cablin, 1 part of setose thistle, 0.5 part of radix aconiti agrestis, 1 part of cordate houttuynia.
Its preparation method comprises the following steps:
(1) with raw material mixing after pulverizing, sieving respectively, get compound; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 5 times of amounts 95%, be heated to 80 ℃ and decoct 1h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times of amounts 95%, is heated to 70 ℃ and decocts 4h;
(4) filter, merging filtrate is concentrated into ethanol and adds 1/20 of total amount, adds the water of 1 times of volume of concentrate, standing 48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control nursery stock damping off.
Embodiment 3
The natural bactericidal agent of control nursery stock damping off is made by the component of following weight portion: 5 parts, mulberry leaf, 4 parts of tarragons, 5 parts of greater celandines, 6 parts of acalypha copperleafs, 5 parts of the coptiss, 2 parts of Dong's sauce back ofs the body, 2 parts of Pogostemon cablin, 2 parts of setose thistles, 1 part of radix aconiti agrestis, 3 parts of cordate houttuynias.
Its preparation method comprises the following steps:
(1) with raw material mixing after pulverizing, sieving respectively, get compound; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 10 times of amounts 95%, be heated to 60 ℃ and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times of amounts 95%, is heated to 50 ℃ and decocts 2h;
(4) filter, merging filtrate is concentrated into ethanol and adds 1/10 of total amount, adds the water of 3 times of volumes of concentrate, standing 24h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control nursery stock damping off.
The natural bactericidal agent of the control nursery stock damping off of embodiment 4 mensuration embodiment 1 to 3 is active to the inhibition of strawberry pathogen
This experiment is in vitro, and the natural bactericidal agent of the control nursery stock damping off of mensuration embodiment 1 to 3 is active to the inhibition of strawberry pathogen, and method and result are as follows:
1. for the examination bacterial classification
(Pythium aphanidermatum (Fds.) Fitz.) is commercially available for pythium spp
(Fusarium solani Mart App.et.Woll) is commercially available for sickle-like bacteria
(Rhizoctonia solani Kuhn) is commercially available for Rhizoctonia solani Kuhn
2. Toxicity Determination
Utilize mycelial growth rate method and spore germination method to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of above-mentioned germ, calculate inhibiting rate by following formula:
3. experimental result
Measure embodiment 1 natural bactericidal agent to the virulence for the examination pathogen with growth rate method, the results are shown in Table 1.
The virulence of table 1 embodiment 1 natural bactericidal agent to pythium spp
The virulence of table 2 embodiment 1 natural bactericidal agent to sickle-like bacteria
The virulence of table 3 embodiment 1 natural bactericidal agent to Rhizoctonia solani Kuhn
Adopt method of regulation in " pesticide field efficacy medicine test criterion ", the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3 tested, diluted concentration be 500-1000 doubly, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment nursery stock damping off of embodiment 1 to 3, various diseases is respectively treated 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3
Claims (5)
1. a natural bactericidal agent of preventing and treating the nursery stock damping off, is characterized in that: made by following component: mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis, cordate houttuynia.
2. a kind of natural bactericidal agent of preventing and treating the nursery stock damping off according to claim 1, it is characterized in that: the component by following weight portion is made: mulberry leaf 4-6 part, tarragon 4-6 part, greater celandine 4-6 part, acalypha copperleaf 4-6 part, coptis 3-5 part, Dong's sauce back of the body 1-3 part, Pogostemon cablin 1-3 part, setose thistle 1-3 part, radix aconiti agrestis 0.5-1.5 part, cordate houttuynia 1-3 part.
3. a kind of natural bactericidal agent of preventing and treating the nursery stock damping off according to claim 1, it is characterized in that: the component by following weight portion is made: 5 parts, mulberry leaf, 5 parts of tarragons, 5 parts of greater celandines, 5 parts of acalypha copperleafs, 4 parts of the coptiss, 2 parts of Dong's sauce back ofs the body, 2 parts of Pogostemon cablin, 2 parts of setose thistles, 1 part of radix aconiti agrestis, 2 parts of cordate houttuynias.
4. preparation method who prevents and treats the natural bactericidal agent of nursery stock damping off is characterized in that: comprise the following steps:
(1) with raw material mixing after pulverizing, sieving respectively, get compound; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 60-80 ℃ and decoct 1-3h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 60-80 ℃ and decocts 1-3h;
(4) filter, merging filtrate is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control nursery stock damping off.
5. the application of the natural bactericidal agent of control nursery stock damping off in control nursery stock damping off is characterized in that: the natural bactericidal agent that will prevent and treat the nursery stock damping off mixes with 500-1000 times of water gaging and is the sterilization microbial inoculum.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310273165.1A CN103380787B (en) | 2013-06-28 | 2013-06-28 | Natural bactericide for preventing and curing nursery stock damping-off and preparation method thereof |
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| CN201310273165.1A CN103380787B (en) | 2013-06-28 | 2013-06-28 | Natural bactericide for preventing and curing nursery stock damping-off and preparation method thereof |
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| CN103380787A true CN103380787A (en) | 2013-11-06 |
| CN103380787B CN103380787B (en) | 2015-04-22 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105309499A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for damping-off disease |
| CN105532768A (en) * | 2016-02-04 | 2016-05-04 | 史运江 | Biopesticide for controlling cabbage bacterial black spot and preparation method |
| CN106993636A (en) * | 2017-05-03 | 2017-08-01 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating safron rust |
| CN107027816A (en) * | 2017-05-03 | 2017-08-11 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating safron rust and its application |
| CN107372666A (en) * | 2017-08-28 | 2017-11-24 | 锦屏县新化乡新化所蔬菜专业合作社 | A kind of composition for preventing and treating vegetable seedling samping off and preparation method thereof |
| CN108064892A (en) * | 2017-11-29 | 2018-05-25 | 沿河土家族自治县双强牧业有限责任公司 | A kind of composition for treating tomato seedling samping off and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309499A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for damping-off disease |
| CN105532768A (en) * | 2016-02-04 | 2016-05-04 | 史运江 | Biopesticide for controlling cabbage bacterial black spot and preparation method |
| CN106993636A (en) * | 2017-05-03 | 2017-08-01 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating safron rust |
| CN107027816A (en) * | 2017-05-03 | 2017-08-11 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating safron rust and its application |
| CN107372666A (en) * | 2017-08-28 | 2017-11-24 | 锦屏县新化乡新化所蔬菜专业合作社 | A kind of composition for preventing and treating vegetable seedling samping off and preparation method thereof |
| CN108064892A (en) * | 2017-11-29 | 2018-05-25 | 沿河土家族自治县双强牧业有限责任公司 | A kind of composition for treating tomato seedling samping off and preparation method thereof |
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| CN103380787B (en) | 2015-04-22 |
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